Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1745 and Pf1N1B4_1746 are separated by 418 nucleotides Pf1N1B4_1746 and Pf1N1B4_1747 are separated by 181 nucleotides Pf1N1B4_1747 and Pf1N1B4_1748 are separated by 5 nucleotides
Pf1N1B4_1745 - Biofilm PGA outer membrane secretin PgaA, at 1,995,429 to 1,997,909
_1745
Pf1N1B4_1746 - GTP-binding protein, at 1,998,328 to 1,999,803
_1746
Pf1N1B4_1747 - hypothetical protein, at 1,999,985 to 2,000,116
_1747
Pf1N1B4_1748 - NADH:ubiquinone oxidoreductase subunit 2 (chain N), at 2,000,122 to 2,000,757
_1748
Position (kb)
1998
1999
2000 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1997.339 kb on - strand, within Pf1N1B4_1745 at 1997.356 kb on + strand, within Pf1N1B4_1745 at 1997.356 kb on + strand, within Pf1N1B4_1745 at 1997.357 kb on - strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.543 kb on - strand, within Pf1N1B4_1745 at 1997.543 kb on - strand, within Pf1N1B4_1745 at 1997.543 kb on - strand, within Pf1N1B4_1745 at 1997.543 kb on - strand, within Pf1N1B4_1745 at 1997.543 kb on - strand, within Pf1N1B4_1745 at 1997.543 kb on - strand, within Pf1N1B4_1745 at 1997.544 kb on + strand, within Pf1N1B4_1745 at 1997.544 kb on + strand, within Pf1N1B4_1745 at 1997.545 kb on - strand, within Pf1N1B4_1745 at 1997.645 kb on - strand, within Pf1N1B4_1745 at 1997.645 kb on - strand, within Pf1N1B4_1745 at 1997.745 kb on + strand at 1997.745 kb on + strand at 1997.745 kb on + strand at 1997.746 kb on - strand at 1997.746 kb on - strand at 1997.764 kb on + strand at 1997.764 kb on + strand at 1997.764 kb on + strand at 1997.764 kb on + strand at 1997.764 kb on + strand at 1997.764 kb on + strand at 1997.765 kb on - strand at 1997.800 kb on + strand at 1997.871 kb on + strand at 1997.885 kb on + strand at 1997.885 kb on + strand at 1997.900 kb on + strand at 1997.900 kb on + strand at 1997.900 kb on + strand at 1997.900 kb on + strand at 1997.923 kb on + strand at 1997.923 kb on + strand at 1997.923 kb on + strand at 1997.923 kb on + strand at 1997.923 kb on + strand at 1997.923 kb on + strand at 1997.923 kb on + strand at 1997.923 kb on + strand at 1997.923 kb on + strand at 1997.924 kb on - strand at 1997.924 kb on - strand at 1997.924 kb on - strand at 1997.924 kb on - strand at 1997.924 kb on - strand at 1997.924 kb on - strand at 1997.924 kb on - strand at 1997.924 kb on - strand at 1997.924 kb on - strand at 1997.924 kb on - strand at 1997.924 kb on - strand at 1998.000 kb on - strand at 1998.033 kb on + strand at 1998.034 kb on - strand at 1998.034 kb on - strand at 1998.047 kb on + strand at 1998.047 kb on + strand at 1998.047 kb on + strand at 1998.047 kb on + strand at 1998.047 kb on + strand at 1998.048 kb on - strand at 1998.048 kb on - strand at 1998.048 kb on - strand at 1998.048 kb on - strand at 1998.048 kb on - strand at 1998.048 kb on - strand at 1998.048 kb on - strand at 1998.048 kb on - strand at 1998.048 kb on - strand at 1998.141 kb on + strand at 1998.141 kb on + strand at 1998.142 kb on - strand at 1998.142 kb on - strand at 1998.290 kb on - strand at 1998.323 kb on - strand at 1998.435 kb on - strand at 1998.435 kb on - strand at 1999.007 kb on + strand, within Pf1N1B4_1746 at 1999.145 kb on + strand, within Pf1N1B4_1746 at 1999.154 kb on + strand, within Pf1N1B4_1746 at 1999.154 kb on + strand, within Pf1N1B4_1746 at 1999.155 kb on - strand, within Pf1N1B4_1746 at 1999.254 kb on + strand, within Pf1N1B4_1746 at 1999.255 kb on - strand, within Pf1N1B4_1746 at 1999.255 kb on - strand, within Pf1N1B4_1746 at 1999.255 kb on - strand, within Pf1N1B4_1746 at 1999.255 kb on - strand, within Pf1N1B4_1746 at 1999.256 kb on + strand, within Pf1N1B4_1746 at 1999.360 kb on + strand, within Pf1N1B4_1746 at 1999.481 kb on + strand, within Pf1N1B4_1746 at 1999.481 kb on + strand, within Pf1N1B4_1746 at 1999.481 kb on + strand, within Pf1N1B4_1746 at 1999.481 kb on + strand, within Pf1N1B4_1746 at 1999.482 kb on - strand, within Pf1N1B4_1746 at 1999.639 kb on + strand, within Pf1N1B4_1746 at 1999.639 kb on + strand, within Pf1N1B4_1746 at 1999.639 kb on + strand, within Pf1N1B4_1746 at 1999.640 kb on - strand, within Pf1N1B4_1746 at 1999.640 kb on - strand, within Pf1N1B4_1746 at 1999.640 kb on - strand, within Pf1N1B4_1746 at 1999.640 kb on - strand, within Pf1N1B4_1746 at 1999.767 kb on - strand at 1999.813 kb on + strand at 1999.814 kb on - strand at 1999.816 kb on + strand at 1999.816 kb on + strand at 1999.817 kb on - strand at 1999.833 kb on + strand at 1999.833 kb on + strand at 1999.834 kb on - strand at 1999.834 kb on - strand at 1999.834 kb on - strand at 1999.844 kb on + strand at 1999.986 kb on + strand at 2000.129 kb on + strand at 2000.129 kb on + strand at 2000.130 kb on - strand at 2000.187 kb on + strand, within Pf1N1B4_1748 at 2000.187 kb on + strand, within Pf1N1B4_1748 at 2000.187 kb on + strand, within Pf1N1B4_1748 at 2000.187 kb on + strand, within Pf1N1B4_1748 at 2000.188 kb on - strand, within Pf1N1B4_1748 at 2000.188 kb on - strand, within Pf1N1B4_1748 at 2000.188 kb on - strand, within Pf1N1B4_1748 at 2000.188 kb on - strand, within Pf1N1B4_1748 at 2000.188 kb on - strand, within Pf1N1B4_1748 at 2000.188 kb on - strand at 2000.188 kb on - strand, within Pf1N1B4_1748 at 2000.188 kb on - strand, within Pf1N1B4_1748 at 2000.188 kb on - strand, within Pf1N1B4_1748 at 2000.189 kb on + strand, within Pf1N1B4_1748 at 2000.189 kb on + strand, within Pf1N1B4_1748 at 2000.189 kb on + strand, within Pf1N1B4_1748 at 2000.189 kb on + strand, within Pf1N1B4_1748 at 2000.190 kb on - strand, within Pf1N1B4_1748 at 2000.457 kb on - strand, within Pf1N1B4_1748 at 2000.472 kb on - strand, within Pf1N1B4_1748 at 2000.578 kb on + strand, within Pf1N1B4_1748 at 2000.579 kb on - strand, within Pf1N1B4_1748 at 2000.645 kb on - strand, within Pf1N1B4_1748 at 2000.690 kb on + strand, within Pf1N1B4_1748 at 2000.690 kb on + strand, within Pf1N1B4_1748 at 2000.690 kb on + strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.758 kb on + strand at 2000.758 kb on + strand at 2000.759 kb on - strand at 2000.761 kb on + strand at 2000.761 kb on + strand at 2000.761 kb on + strand at 2000.761 kb on + strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 1,997,339 - Pf1N1B4_1745 0.77 +0.0 1,997,356 + Pf1N1B4_1745 0.78 +0.0 1,997,356 + Pf1N1B4_1745 0.78 +0.0 1,997,357 - Pf1N1B4_1745 0.78 +0.0 1,997,542 + Pf1N1B4_1745 0.85 -0.4 1,997,542 + Pf1N1B4_1745 0.85 +0.0 1,997,542 + Pf1N1B4_1745 0.85 +0.0 1,997,542 + Pf1N1B4_1745 0.85 +0.0 1,997,542 + Pf1N1B4_1745 0.85 +0.0 1,997,542 + Pf1N1B4_1745 0.85 +0.0 1,997,542 + Pf1N1B4_1745 0.85 +0.0 1,997,542 + Pf1N1B4_1745 0.85 +0.0 1,997,542 + Pf1N1B4_1745 0.85 +0.0 1,997,542 + Pf1N1B4_1745 0.85 -1.7 1,997,542 + Pf1N1B4_1745 0.85 +0.0 1,997,542 + Pf1N1B4_1745 0.85 +0.0 1,997,542 + Pf1N1B4_1745 0.85 -1.7 1,997,542 + Pf1N1B4_1745 0.85 -2.3 1,997,542 + Pf1N1B4_1745 0.85 +0.0 1,997,542 + Pf1N1B4_1745 0.85 +0.0 1,997,542 + Pf1N1B4_1745 0.85 -2.4 1,997,542 + Pf1N1B4_1745 0.85 +0.0 1,997,543 - Pf1N1B4_1745 0.85 +0.0 1,997,543 - Pf1N1B4_1745 0.85 +0.0 1,997,543 - Pf1N1B4_1745 0.85 -1.6 1,997,543 - Pf1N1B4_1745 0.85 -3.4 1,997,543 - Pf1N1B4_1745 0.85 +2.8 1,997,543 - Pf1N1B4_1745 0.85 +0.0 1,997,544 + Pf1N1B4_1745 0.85 +0.0 1,997,544 + Pf1N1B4_1745 0.85 +0.0 1,997,545 - Pf1N1B4_1745 0.85 +0.0 1,997,645 - Pf1N1B4_1745 0.89 +0.0 1,997,645 - Pf1N1B4_1745 0.89 +0.0 1,997,745 + -1.9 1,997,745 + +0.0 1,997,745 + -1.3 1,997,746 - +0.0 1,997,746 - +0.0 1,997,764 + +0.0 1,997,764 + +0.0 1,997,764 + -1.2 1,997,764 + +0.0 1,997,764 + +0.0 1,997,764 + +0.0 1,997,765 - +0.0 1,997,800 + -1.0 1,997,871 + +0.0 1,997,885 + +0.0 1,997,885 + -2.3 1,997,900 + -2.1 1,997,900 + +0.0 1,997,900 + +0.0 1,997,900 + -2.0 1,997,923 + +0.0 1,997,923 + +0.0 1,997,923 + +0.0 1,997,923 + +0.0 1,997,923 + -2.4 1,997,923 + +0.0 1,997,923 + +0.0 1,997,923 + -0.3 1,997,923 + +0.0 1,997,924 - +0.0 1,997,924 - +0.0 1,997,924 - -1.2 1,997,924 - -1.5 1,997,924 - +0.0 1,997,924 - -4.9 1,997,924 - +0.0 1,997,924 - +0.0 1,997,924 - +0.0 1,997,924 - -1.9 1,997,924 - +0.0 1,998,000 - -0.8 1,998,033 + +0.0 1,998,034 - +0.0 1,998,034 - +0.0 1,998,047 + +0.0 1,998,047 + +0.0 1,998,047 + -1.4 1,998,047 + -1.1 1,998,047 + +0.0 1,998,048 - -1.3 1,998,048 - +0.0 1,998,048 - +0.0 1,998,048 - +0.0 1,998,048 - +0.0 1,998,048 - -1.0 1,998,048 - -2.5 1,998,048 - -4.9 1,998,048 - +0.0 1,998,141 + +0.0 1,998,141 + -0.8 1,998,142 - +0.0 1,998,142 - +0.0 1,998,290 - +0.0 1,998,323 - +0.0 1,998,435 - -1.9 1,998,435 - -4.1 1,999,007 + Pf1N1B4_1746 0.46 +0.0 1,999,145 + Pf1N1B4_1746 0.55 -1.5 1,999,154 + Pf1N1B4_1746 0.56 +0.0 1,999,154 + Pf1N1B4_1746 0.56 +0.0 1,999,155 - Pf1N1B4_1746 0.56 +0.0 1,999,254 + Pf1N1B4_1746 0.63 +0.0 1,999,255 - Pf1N1B4_1746 0.63 +0.0 1,999,255 - Pf1N1B4_1746 0.63 -1.9 1,999,255 - Pf1N1B4_1746 0.63 -1.3 1,999,255 - Pf1N1B4_1746 0.63 +0.0 1,999,256 + Pf1N1B4_1746 0.63 +0.0 1,999,360 + Pf1N1B4_1746 0.70 -1.0 1,999,481 + Pf1N1B4_1746 0.78 +0.0 1,999,481 + Pf1N1B4_1746 0.78 +0.0 1,999,481 + Pf1N1B4_1746 0.78 +0.0 1,999,481 + Pf1N1B4_1746 0.78 +0.0 1,999,482 - Pf1N1B4_1746 0.78 +0.9 1,999,639 + Pf1N1B4_1746 0.89 -1.7 1,999,639 + Pf1N1B4_1746 0.89 +0.0 1,999,639 + Pf1N1B4_1746 0.89 +0.0 1,999,640 - Pf1N1B4_1746 0.89 +0.0 1,999,640 - Pf1N1B4_1746 0.89 +0.0 1,999,640 - Pf1N1B4_1746 0.89 +0.0 1,999,640 - Pf1N1B4_1746 0.89 -0.7 1,999,767 - +0.0 1,999,813 + +0.0 1,999,814 - +0.0 1,999,816 + +0.0 1,999,816 + -0.7 1,999,817 - +0.0 1,999,833 + +0.0 1,999,833 + -4.2 1,999,834 - +0.0 1,999,834 - -1.7 1,999,834 - +0.0 1,999,844 + -1.8 1,999,986 + +0.0 2,000,129 + +0.0 2,000,129 + +0.0 2,000,130 - +0.0 2,000,187 + Pf1N1B4_1748 0.10 +0.1 2,000,187 + Pf1N1B4_1748 0.10 +0.0 2,000,187 + Pf1N1B4_1748 0.10 +0.0 2,000,187 + Pf1N1B4_1748 0.10 +0.0 2,000,188 - Pf1N1B4_1748 0.10 +0.0 2,000,188 - Pf1N1B4_1748 0.10 +0.0 2,000,188 - Pf1N1B4_1748 0.10 +0.0 2,000,188 - Pf1N1B4_1748 0.10 +0.0 2,000,188 - Pf1N1B4_1748 0.10 +0.0 2,000,188 - +0.0 2,000,188 - Pf1N1B4_1748 0.10 +0.0 2,000,188 - Pf1N1B4_1748 0.10 +0.0 2,000,188 - Pf1N1B4_1748 0.10 +0.0 2,000,189 + Pf1N1B4_1748 0.11 +0.0 2,000,189 + Pf1N1B4_1748 0.11 -1.8 2,000,189 + Pf1N1B4_1748 0.11 +0.0 2,000,189 + Pf1N1B4_1748 0.11 +0.0 2,000,190 - Pf1N1B4_1748 0.11 +0.0 2,000,457 - Pf1N1B4_1748 0.53 +0.0 2,000,472 - Pf1N1B4_1748 0.55 +0.0 2,000,578 + Pf1N1B4_1748 0.72 +0.0 2,000,579 - Pf1N1B4_1748 0.72 +0.0 2,000,645 - Pf1N1B4_1748 0.82 +0.0 2,000,690 + Pf1N1B4_1748 0.89 +0.0 2,000,690 + Pf1N1B4_1748 0.89 -1.5 2,000,690 + Pf1N1B4_1748 0.89 -1.0 2,000,691 - Pf1N1B4_1748 0.89 +0.0 2,000,691 - Pf1N1B4_1748 0.89 +0.0 2,000,691 - Pf1N1B4_1748 0.89 +0.0 2,000,691 - Pf1N1B4_1748 0.89 +0.0 2,000,691 - Pf1N1B4_1748 0.89 +0.0 2,000,691 - Pf1N1B4_1748 0.89 -2.1 2,000,758 + +0.0 2,000,758 + +0.0 2,000,759 - +0.0 2,000,761 + -3.2 2,000,761 + +0.0 2,000,761 + +0.0 2,000,761 + +0.0 2,000,762 - +0.0 2,000,762 - -1.9 2,000,762 - +0.0 2,000,762 - +0.0 2,000,762 - +0.0 2,000,762 - +0.0 2,000,762 - +0.0 2,000,762 - -0.8 2,000,762 - +0.0 2,000,762 - +0.0 2,000,762 - +0.0 2,000,762 - +0.0 2,000,762 - +0.0 2,000,762 - +0.0
Or see this region's nucleotide sequence