Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_152 and Pf1N1B4_153 are separated by 213 nucleotides Pf1N1B4_153 and Pf1N1B4_154 are separated by 139 nucleotides
Pf1N1B4_152 - ABC transporter, permease protein, at 194,143 to 195,264
_152
Pf1N1B4_153 - Glucoamylase (EC 3.2.1.3), at 195,478 to 197,301
_153
Pf1N1B4_154 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 197,441 to 197,815
_154
Position (kb) 195
196
197
198 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 194.552 kb on + strand, within Pf1N1B4_152 at 194.718 kb on - strand, within Pf1N1B4_152 at 194.718 kb on - strand, within Pf1N1B4_152 at 194.725 kb on + strand, within Pf1N1B4_152 at 194.725 kb on + strand, within Pf1N1B4_152 at 194.856 kb on + strand at 194.857 kb on - strand, within Pf1N1B4_152 at 194.902 kb on + strand, within Pf1N1B4_152 at 195.024 kb on + strand, within Pf1N1B4_152 at 195.025 kb on - strand, within Pf1N1B4_152 at 195.445 kb on - strand at 195.449 kb on - strand at 195.871 kb on + strand, within Pf1N1B4_153 at 195.916 kb on + strand, within Pf1N1B4_153 at 195.917 kb on - strand, within Pf1N1B4_153 at 195.969 kb on - strand, within Pf1N1B4_153 at 196.313 kb on - strand, within Pf1N1B4_153 at 196.313 kb on - strand, within Pf1N1B4_153 at 196.420 kb on + strand, within Pf1N1B4_153 at 196.516 kb on + strand at 196.564 kb on + strand, within Pf1N1B4_153 at 196.564 kb on + strand, within Pf1N1B4_153 at 196.564 kb on + strand, within Pf1N1B4_153 at 196.564 kb on + strand, within Pf1N1B4_153 at 196.565 kb on - strand at 196.813 kb on + strand, within Pf1N1B4_153 at 196.813 kb on + strand at 196.813 kb on + strand, within Pf1N1B4_153 at 196.813 kb on + strand, within Pf1N1B4_153 at 197.077 kb on + strand, within Pf1N1B4_153 at 197.077 kb on + strand, within Pf1N1B4_153 at 197.333 kb on + strand at 197.333 kb on + strand at 197.333 kb on + strand at 197.334 kb on - strand at 197.334 kb on - strand at 197.334 kb on - strand at 197.334 kb on - strand at 197.334 kb on - strand at 197.563 kb on - strand, within Pf1N1B4_154 at 197.810 kb on + strand at 197.810 kb on + strand at 197.810 kb on + strand at 197.820 kb on + strand at 197.880 kb on - strand at 197.956 kb on - strand at 198.086 kb on + strand at 198.086 kb on + strand at 198.086 kb on + strand at 198.086 kb on + strand at 198.086 kb on + strand at 198.087 kb on - strand at 198.112 kb on - strand at 198.163 kb on - strand at 198.183 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 194,552 + Pf1N1B4_152 0.36 -1.4 194,718 - Pf1N1B4_152 0.51 -1.7 194,718 - Pf1N1B4_152 0.51 -0.1 194,725 + Pf1N1B4_152 0.52 -0.1 194,725 + Pf1N1B4_152 0.52 -0.1 194,856 + -4.1 194,857 - Pf1N1B4_152 0.64 -1.2 194,902 + Pf1N1B4_152 0.68 -1.6 195,024 + Pf1N1B4_152 0.79 -0.1 195,025 - Pf1N1B4_152 0.79 -0.1 195,445 - -2.9 195,449 - -0.1 195,871 + Pf1N1B4_153 0.22 -0.1 195,916 + Pf1N1B4_153 0.24 -0.1 195,917 - Pf1N1B4_153 0.24 -0.1 195,969 - Pf1N1B4_153 0.27 -0.1 196,313 - Pf1N1B4_153 0.46 -0.1 196,313 - Pf1N1B4_153 0.46 -3.3 196,420 + Pf1N1B4_153 0.52 -0.1 196,516 + -1.8 196,564 + Pf1N1B4_153 0.60 -0.1 196,564 + Pf1N1B4_153 0.60 -0.1 196,564 + Pf1N1B4_153 0.60 -3.5 196,564 + Pf1N1B4_153 0.60 -0.1 196,565 - -0.1 196,813 + Pf1N1B4_153 0.73 -2.7 196,813 + -2.3 196,813 + Pf1N1B4_153 0.73 -3.3 196,813 + Pf1N1B4_153 0.73 -0.1 197,077 + Pf1N1B4_153 0.88 -0.1 197,077 + Pf1N1B4_153 0.88 -4.3 197,333 + -0.1 197,333 + -0.1 197,333 + -0.1 197,334 - -1.7 197,334 - -0.1 197,334 - -1.0 197,334 - -0.1 197,334 - -1.8 197,563 - Pf1N1B4_154 0.33 -0.1 197,810 + -1.4 197,810 + -0.1 197,810 + -0.1 197,820 + -0.1 197,880 - +0.2 197,956 - -1.7 198,086 + -0.1 198,086 + -0.1 198,086 + -0.1 198,086 + -1.0 198,086 + -0.1 198,087 - -1.4 198,112 - -0.1 198,163 - -0.1 198,183 + -0.8
Or see this region's nucleotide sequence