Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1437 and Pf1N1B4_1438 are separated by 10 nucleotides Pf1N1B4_1438 and Pf1N1B4_1439 overlap by 4 nucleotides Pf1N1B4_1439 and Pf1N1B4_1440 overlap by 1 nucleotides
Pf1N1B4_1437 - Topoisomerase IV subunit A (EC 5.99.1.-), at 1,647,746 to 1,650,013
_1437
Pf1N1B4_1438 - Transporter, at 1,650,024 to 1,650,545
_1438
Pf1N1B4_1439 - FIG00953138: hypothetical protein, at 1,650,542 to 1,651,531
_1439
Pf1N1B4_1440 - Topoisomerase IV subunit B (EC 5.99.1.-), at 1,651,531 to 1,653,435
_1440
Position (kb) 1650
1651
1652 Strain fitness (log2 ratio) -1
0
1 at 1649.979 kb on - strand at 1650.069 kb on - strand at 1650.096 kb on - strand, within Pf1N1B4_1438 at 1650.626 kb on - strand at 1650.699 kb on + strand, within Pf1N1B4_1439 at 1650.919 kb on + strand, within Pf1N1B4_1439 at 1650.920 kb on - strand at 1650.920 kb on - strand, within Pf1N1B4_1439 at 1650.920 kb on - strand, within Pf1N1B4_1439 at 1650.920 kb on - strand, within Pf1N1B4_1439 at 1651.051 kb on + strand, within Pf1N1B4_1439 at 1651.052 kb on + strand, within Pf1N1B4_1439 at 1651.052 kb on + strand, within Pf1N1B4_1439 at 1651.053 kb on - strand, within Pf1N1B4_1439 at 1651.053 kb on - strand, within Pf1N1B4_1439 at 1651.058 kb on - strand, within Pf1N1B4_1439 at 1651.058 kb on - strand, within Pf1N1B4_1439 at 1651.058 kb on - strand, within Pf1N1B4_1439 at 1651.164 kb on + strand, within Pf1N1B4_1439 at 1651.165 kb on - strand, within Pf1N1B4_1439 at 1651.263 kb on - strand at 1651.318 kb on + strand, within Pf1N1B4_1439 at 1651.319 kb on - strand, within Pf1N1B4_1439 at 1651.342 kb on - strand, within Pf1N1B4_1439
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 1,649,979 - -0.0 1,650,069 - -0.0 1,650,096 - Pf1N1B4_1438 0.14 -0.0 1,650,626 - -0.0 1,650,699 + Pf1N1B4_1439 0.16 -0.0 1,650,919 + Pf1N1B4_1439 0.38 -1.5 1,650,920 - -0.3 1,650,920 - Pf1N1B4_1439 0.38 -0.0 1,650,920 - Pf1N1B4_1439 0.38 -0.0 1,650,920 - Pf1N1B4_1439 0.38 -0.0 1,651,051 + Pf1N1B4_1439 0.51 -0.0 1,651,052 + Pf1N1B4_1439 0.52 -1.6 1,651,052 + Pf1N1B4_1439 0.52 -0.0 1,651,053 - Pf1N1B4_1439 0.52 -0.0 1,651,053 - Pf1N1B4_1439 0.52 -0.0 1,651,058 - Pf1N1B4_1439 0.52 -0.2 1,651,058 - Pf1N1B4_1439 0.52 -0.0 1,651,058 - Pf1N1B4_1439 0.52 -0.0 1,651,164 + Pf1N1B4_1439 0.63 -0.0 1,651,165 - Pf1N1B4_1439 0.63 -0.9 1,651,263 - -0.4 1,651,318 + Pf1N1B4_1439 0.78 -0.0 1,651,319 - Pf1N1B4_1439 0.78 -0.0 1,651,342 - Pf1N1B4_1439 0.81 -0.0
Or see this region's nucleotide sequence