Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1353 and Pf1N1B4_1354 are separated by 67 nucleotides Pf1N1B4_1354 and Pf1N1B4_1355 are separated by 41 nucleotides
Pf1N1B4_1353 - Urease alpha subunit (EC 3.5.1.5), at 1,547,911 to 1,549,611
_1353
Pf1N1B4_1354 - hypothetical protein, at 1,549,679 to 1,552,135
_1354
Pf1N1B4_1355 - hypothetical protein, at 1,552,177 to 1,554,810
_1355
Position (kb)
1549
1550
1551
1552
1553 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1548.718 kb on + strand, within Pf1N1B4_1353 at 1548.719 kb on - strand, within Pf1N1B4_1353 at 1548.719 kb on - strand, within Pf1N1B4_1353 at 1548.785 kb on + strand, within Pf1N1B4_1353 at 1548.786 kb on - strand, within Pf1N1B4_1353 at 1548.786 kb on - strand, within Pf1N1B4_1353 at 1548.786 kb on - strand, within Pf1N1B4_1353 at 1549.114 kb on + strand, within Pf1N1B4_1353 at 1549.115 kb on - strand, within Pf1N1B4_1353 at 1549.115 kb on - strand, within Pf1N1B4_1353 at 1549.126 kb on + strand, within Pf1N1B4_1353 at 1549.126 kb on + strand, within Pf1N1B4_1353 at 1549.126 kb on + strand, within Pf1N1B4_1353 at 1549.126 kb on + strand, within Pf1N1B4_1353 at 1549.233 kb on - strand, within Pf1N1B4_1353 at 1549.495 kb on + strand at 1549.496 kb on - strand at 1549.496 kb on - strand at 1549.528 kb on + strand at 1549.528 kb on + strand at 1549.528 kb on + strand at 1549.528 kb on + strand at 1549.528 kb on + strand at 1549.529 kb on - strand at 1549.529 kb on - strand at 1549.556 kb on + strand at 1549.597 kb on + strand at 1549.597 kb on + strand at 1549.597 kb on + strand at 1549.598 kb on - strand at 1549.620 kb on + strand at 1549.620 kb on + strand at 1549.620 kb on + strand at 1549.621 kb on - strand at 1549.736 kb on - strand at 1549.739 kb on + strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.791 kb on + strand at 1549.919 kb on + strand at 1549.919 kb on + strand at 1549.935 kb on - strand, within Pf1N1B4_1354 at 1549.935 kb on - strand, within Pf1N1B4_1354 at 1550.179 kb on - strand, within Pf1N1B4_1354 at 1550.179 kb on - strand, within Pf1N1B4_1354 at 1550.374 kb on - strand, within Pf1N1B4_1354 at 1550.374 kb on - strand, within Pf1N1B4_1354 at 1550.374 kb on - strand, within Pf1N1B4_1354 at 1550.374 kb on - strand, within Pf1N1B4_1354 at 1550.374 kb on - strand, within Pf1N1B4_1354 at 1550.632 kb on - strand, within Pf1N1B4_1354 at 1550.751 kb on + strand, within Pf1N1B4_1354 at 1550.751 kb on + strand, within Pf1N1B4_1354 at 1550.752 kb on - strand, within Pf1N1B4_1354 at 1550.752 kb on - strand, within Pf1N1B4_1354 at 1550.752 kb on - strand, within Pf1N1B4_1354 at 1550.752 kb on - strand, within Pf1N1B4_1354 at 1550.752 kb on - strand, within Pf1N1B4_1354 at 1550.752 kb on - strand, within Pf1N1B4_1354 at 1550.754 kb on - strand, within Pf1N1B4_1354 at 1550.754 kb on - strand, within Pf1N1B4_1354 at 1550.896 kb on + strand, within Pf1N1B4_1354 at 1550.896 kb on + strand, within Pf1N1B4_1354 at 1550.897 kb on - strand, within Pf1N1B4_1354 at 1550.897 kb on - strand, within Pf1N1B4_1354 at 1550.897 kb on - strand, within Pf1N1B4_1354 at 1550.897 kb on - strand, within Pf1N1B4_1354 at 1550.897 kb on - strand, within Pf1N1B4_1354 at 1550.897 kb on - strand, within Pf1N1B4_1354 at 1550.958 kb on - strand, within Pf1N1B4_1354 at 1550.958 kb on - strand, within Pf1N1B4_1354 at 1551.030 kb on - strand, within Pf1N1B4_1354 at 1551.410 kb on - strand, within Pf1N1B4_1354 at 1551.616 kb on - strand, within Pf1N1B4_1354 at 1551.618 kb on - strand, within Pf1N1B4_1354 at 1551.669 kb on + strand, within Pf1N1B4_1354 at 1551.670 kb on - strand, within Pf1N1B4_1354 at 1551.712 kb on - strand, within Pf1N1B4_1354 at 1551.743 kb on + strand, within Pf1N1B4_1354 at 1551.743 kb on + strand, within Pf1N1B4_1354 at 1551.870 kb on + strand, within Pf1N1B4_1354 at 1551.870 kb on + strand, within Pf1N1B4_1354 at 1551.870 kb on + strand, within Pf1N1B4_1354 at 1551.870 kb on + strand, within Pf1N1B4_1354 at 1551.870 kb on + strand, within Pf1N1B4_1354 at 1551.870 kb on + strand, within Pf1N1B4_1354 at 1551.870 kb on + strand, within Pf1N1B4_1354 at 1551.871 kb on - strand, within Pf1N1B4_1354 at 1551.871 kb on - strand, within Pf1N1B4_1354 at 1551.871 kb on - strand, within Pf1N1B4_1354 at 1551.871 kb on - strand, within Pf1N1B4_1354 at 1551.872 kb on + strand, within Pf1N1B4_1354 at 1551.872 kb on + strand, within Pf1N1B4_1354 at 1551.872 kb on + strand, within Pf1N1B4_1354 at 1551.872 kb on + strand, within Pf1N1B4_1354 at 1551.872 kb on + strand, within Pf1N1B4_1354 at 1551.872 kb on + strand, within Pf1N1B4_1354 at 1551.872 kb on + strand, within Pf1N1B4_1354 at 1551.873 kb on - strand, within Pf1N1B4_1354 at 1551.873 kb on - strand, within Pf1N1B4_1354 at 1551.873 kb on - strand, within Pf1N1B4_1354 at 1551.873 kb on - strand, within Pf1N1B4_1354 at 1551.874 kb on - strand, within Pf1N1B4_1354 at 1552.095 kb on + strand at 1552.110 kb on + strand at 1552.111 kb on - strand at 1552.111 kb on - strand at 1552.111 kb on - strand at 1552.111 kb on - strand at 1552.111 kb on - strand at 1552.112 kb on + strand at 1552.122 kb on + strand at 1552.123 kb on - strand at 1552.139 kb on + strand at 1552.139 kb on + strand at 1552.139 kb on + strand at 1552.139 kb on + strand at 1552.139 kb on + strand at 1552.140 kb on - strand at 1552.331 kb on + strand at 1552.480 kb on + strand, within Pf1N1B4_1355 at 1552.480 kb on + strand, within Pf1N1B4_1355 at 1552.481 kb on - strand, within Pf1N1B4_1355 at 1552.481 kb on - strand, within Pf1N1B4_1355 at 1552.521 kb on + strand, within Pf1N1B4_1355 at 1552.521 kb on + strand, within Pf1N1B4_1355 at 1552.521 kb on + strand, within Pf1N1B4_1355 at 1552.521 kb on + strand, within Pf1N1B4_1355 at 1552.521 kb on + strand, within Pf1N1B4_1355 at 1552.521 kb on + strand, within Pf1N1B4_1355 at 1552.521 kb on + strand, within Pf1N1B4_1355 at 1552.521 kb on + strand, within Pf1N1B4_1355 at 1552.522 kb on - strand, within Pf1N1B4_1355 at 1552.522 kb on - strand, within Pf1N1B4_1355 at 1552.522 kb on - strand, within Pf1N1B4_1355 at 1552.642 kb on + strand, within Pf1N1B4_1355 at 1552.729 kb on - strand, within Pf1N1B4_1355 at 1552.729 kb on - strand, within Pf1N1B4_1355 at 1552.742 kb on - strand, within Pf1N1B4_1355 at 1552.788 kb on + strand, within Pf1N1B4_1355 at 1552.816 kb on + strand, within Pf1N1B4_1355 at 1552.817 kb on - strand, within Pf1N1B4_1355 at 1552.817 kb on - strand, within Pf1N1B4_1355 at 1552.955 kb on + strand, within Pf1N1B4_1355 at 1552.956 kb on - strand, within Pf1N1B4_1355 at 1552.956 kb on - strand, within Pf1N1B4_1355 at 1553.021 kb on + strand, within Pf1N1B4_1355 at 1553.120 kb on + strand, within Pf1N1B4_1355 at 1553.121 kb on - strand, within Pf1N1B4_1355
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 1,548,718 + Pf1N1B4_1353 0.47 -0.0 1,548,719 - Pf1N1B4_1353 0.48 -5.5 1,548,719 - Pf1N1B4_1353 0.48 -0.0 1,548,785 + Pf1N1B4_1353 0.51 -0.0 1,548,786 - Pf1N1B4_1353 0.51 -0.0 1,548,786 - Pf1N1B4_1353 0.51 -4.5 1,548,786 - Pf1N1B4_1353 0.51 -0.0 1,549,114 + Pf1N1B4_1353 0.71 -0.5 1,549,115 - Pf1N1B4_1353 0.71 -0.0 1,549,115 - Pf1N1B4_1353 0.71 -0.0 1,549,126 + Pf1N1B4_1353 0.71 -2.9 1,549,126 + Pf1N1B4_1353 0.71 -0.0 1,549,126 + Pf1N1B4_1353 0.71 -0.0 1,549,126 + Pf1N1B4_1353 0.71 -0.0 1,549,233 - Pf1N1B4_1353 0.78 -1.3 1,549,495 + -0.0 1,549,496 - -0.0 1,549,496 - -0.0 1,549,528 + -0.8 1,549,528 + -0.0 1,549,528 + -0.0 1,549,528 + -0.0 1,549,528 + -0.0 1,549,529 - -0.0 1,549,529 - -2.7 1,549,556 + -0.5 1,549,597 + -2.5 1,549,597 + -2.3 1,549,597 + -0.0 1,549,598 - -0.0 1,549,620 + -0.0 1,549,620 + -0.0 1,549,620 + -0.0 1,549,621 - -0.0 1,549,736 - -0.0 1,549,739 + -0.0 1,549,740 - -2.0 1,549,740 - -0.0 1,549,740 - -2.3 1,549,740 - -0.0 1,549,740 - -0.0 1,549,740 - -1.3 1,549,740 - -5.0 1,549,740 - -5.1 1,549,740 - -0.0 1,549,791 + -0.0 1,549,919 + -4.6 1,549,919 + -0.0 1,549,935 - Pf1N1B4_1354 0.10 -0.0 1,549,935 - Pf1N1B4_1354 0.10 -0.0 1,550,179 - Pf1N1B4_1354 0.20 -3.2 1,550,179 - Pf1N1B4_1354 0.20 -0.0 1,550,374 - Pf1N1B4_1354 0.28 -0.0 1,550,374 - Pf1N1B4_1354 0.28 -1.1 1,550,374 - Pf1N1B4_1354 0.28 -2.3 1,550,374 - Pf1N1B4_1354 0.28 -0.0 1,550,374 - Pf1N1B4_1354 0.28 -0.0 1,550,632 - Pf1N1B4_1354 0.39 -0.0 1,550,751 + Pf1N1B4_1354 0.44 -0.0 1,550,751 + Pf1N1B4_1354 0.44 +0.6 1,550,752 - Pf1N1B4_1354 0.44 -0.0 1,550,752 - Pf1N1B4_1354 0.44 -0.0 1,550,752 - Pf1N1B4_1354 0.44 -0.0 1,550,752 - Pf1N1B4_1354 0.44 -0.4 1,550,752 - Pf1N1B4_1354 0.44 -0.0 1,550,752 - Pf1N1B4_1354 0.44 -0.0 1,550,754 - Pf1N1B4_1354 0.44 -0.0 1,550,754 - Pf1N1B4_1354 0.44 -0.0 1,550,896 + Pf1N1B4_1354 0.50 -0.0 1,550,896 + Pf1N1B4_1354 0.50 -3.7 1,550,897 - Pf1N1B4_1354 0.50 -0.0 1,550,897 - Pf1N1B4_1354 0.50 -0.0 1,550,897 - Pf1N1B4_1354 0.50 -0.5 1,550,897 - Pf1N1B4_1354 0.50 -2.3 1,550,897 - Pf1N1B4_1354 0.50 -1.9 1,550,897 - Pf1N1B4_1354 0.50 -0.0 1,550,958 - Pf1N1B4_1354 0.52 -2.8 1,550,958 - Pf1N1B4_1354 0.52 -1.5 1,551,030 - Pf1N1B4_1354 0.55 -1.1 1,551,410 - Pf1N1B4_1354 0.70 -0.0 1,551,616 - Pf1N1B4_1354 0.79 -0.0 1,551,618 - Pf1N1B4_1354 0.79 -0.0 1,551,669 + Pf1N1B4_1354 0.81 -0.0 1,551,670 - Pf1N1B4_1354 0.81 -0.0 1,551,712 - Pf1N1B4_1354 0.83 -0.0 1,551,743 + Pf1N1B4_1354 0.84 -2.3 1,551,743 + Pf1N1B4_1354 0.84 -0.7 1,551,870 + Pf1N1B4_1354 0.89 -0.0 1,551,870 + Pf1N1B4_1354 0.89 -1.1 1,551,870 + Pf1N1B4_1354 0.89 -0.0 1,551,870 + Pf1N1B4_1354 0.89 -1.9 1,551,870 + Pf1N1B4_1354 0.89 -1.1 1,551,870 + Pf1N1B4_1354 0.89 -0.0 1,551,870 + Pf1N1B4_1354 0.89 -0.0 1,551,871 - Pf1N1B4_1354 0.89 -2.8 1,551,871 - Pf1N1B4_1354 0.89 -1.5 1,551,871 - Pf1N1B4_1354 0.89 -0.0 1,551,871 - Pf1N1B4_1354 0.89 -1.1 1,551,872 + Pf1N1B4_1354 0.89 -0.3 1,551,872 + Pf1N1B4_1354 0.89 -0.9 1,551,872 + Pf1N1B4_1354 0.89 -2.0 1,551,872 + Pf1N1B4_1354 0.89 -0.0 1,551,872 + Pf1N1B4_1354 0.89 -0.0 1,551,872 + Pf1N1B4_1354 0.89 -0.0 1,551,872 + Pf1N1B4_1354 0.89 -1.4 1,551,873 - Pf1N1B4_1354 0.89 -0.0 1,551,873 - Pf1N1B4_1354 0.89 -2.0 1,551,873 - Pf1N1B4_1354 0.89 -1.6 1,551,873 - Pf1N1B4_1354 0.89 -0.7 1,551,874 - Pf1N1B4_1354 0.89 -0.0 1,552,095 + -0.0 1,552,110 + -1.8 1,552,111 - -0.0 1,552,111 - -0.0 1,552,111 - -0.0 1,552,111 - -0.0 1,552,111 - -0.6 1,552,112 + -0.0 1,552,122 + -0.0 1,552,123 - -0.0 1,552,139 + -0.9 1,552,139 + -2.6 1,552,139 + -2.4 1,552,139 + -0.0 1,552,139 + -0.0 1,552,140 - -0.0 1,552,331 + -0.0 1,552,480 + Pf1N1B4_1355 0.12 -0.0 1,552,480 + Pf1N1B4_1355 0.12 -0.0 1,552,481 - Pf1N1B4_1355 0.12 -0.0 1,552,481 - Pf1N1B4_1355 0.12 -0.0 1,552,521 + Pf1N1B4_1355 0.13 -1.4 1,552,521 + Pf1N1B4_1355 0.13 -0.0 1,552,521 + Pf1N1B4_1355 0.13 -0.2 1,552,521 + Pf1N1B4_1355 0.13 -0.7 1,552,521 + Pf1N1B4_1355 0.13 -0.0 1,552,521 + Pf1N1B4_1355 0.13 -0.0 1,552,521 + Pf1N1B4_1355 0.13 -0.0 1,552,521 + Pf1N1B4_1355 0.13 -2.6 1,552,522 - Pf1N1B4_1355 0.13 -0.0 1,552,522 - Pf1N1B4_1355 0.13 -0.0 1,552,522 - Pf1N1B4_1355 0.13 -3.3 1,552,642 + Pf1N1B4_1355 0.18 -0.0 1,552,729 - Pf1N1B4_1355 0.21 -0.0 1,552,729 - Pf1N1B4_1355 0.21 -0.0 1,552,742 - Pf1N1B4_1355 0.21 -0.0 1,552,788 + Pf1N1B4_1355 0.23 -2.3 1,552,816 + Pf1N1B4_1355 0.24 -4.5 1,552,817 - Pf1N1B4_1355 0.24 -0.0 1,552,817 - Pf1N1B4_1355 0.24 -0.0 1,552,955 + Pf1N1B4_1355 0.30 -0.0 1,552,956 - Pf1N1B4_1355 0.30 -0.0 1,552,956 - Pf1N1B4_1355 0.30 -0.0 1,553,021 + Pf1N1B4_1355 0.32 -0.0 1,553,120 + Pf1N1B4_1355 0.36 -0.0 1,553,121 - Pf1N1B4_1355 0.36 -0.0
Or see this region's nucleotide sequence