Experiment: m.b. Nalidixic 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c21970 and PGA1_c21980 are separated by 3 nucleotides PGA1_c21980 and PGA1_c21990 are separated by 194 nucleotides PGA1_c21990 and PGA1_c22000 are separated by 153 nucleotides PGA1_c22000 and PGA1_c22010 are separated by 74 nucleotides
PGA1_c21970: PGA1_c21970 - glutamyl-tRNA(Gln) amidotransferase subunit A, at 2,277,995 to 2,279,482
_c21970
PGA1_c21980: PGA1_c21980 - aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C, at 2,279,486 to 2,279,773
_c21980
PGA1_c21990: PGA1_c21990 - putative metal-dependent hydrolase, at 2,279,968 to 2,280,660
_c21990
PGA1_c22000: PGA1_c22000 - putative DNA-3-methyladenine glycosylase 1, at 2,280,814 to 2,281,437
_c22000
PGA1_c22010: PGA1_c22010 - tRNA-specific adenosine deaminase TadA, at 2,281,512 to 2,281,973
_c22010
Position (kb)
2279
2280
2281 Strain fitness (log2 ratio)
-2
-1
0
1 at 2279.879 kb on - strand at 2279.906 kb on + strand at 2279.906 kb on + strand at 2279.912 kb on + strand at 2279.912 kb on + strand at 2279.912 kb on + strand at 2279.912 kb on + strand at 2279.920 kb on - strand at 2279.920 kb on - strand at 2279.938 kb on + strand at 2279.938 kb on + strand at 2279.986 kb on + strand at 2280.053 kb on + strand, within PGA1_c21990 at 2280.179 kb on + strand, within PGA1_c21990 at 2280.214 kb on - strand, within PGA1_c21990 at 2280.233 kb on + strand, within PGA1_c21990 at 2280.312 kb on - strand, within PGA1_c21990 at 2280.336 kb on - strand, within PGA1_c21990 at 2280.338 kb on - strand, within PGA1_c21990 at 2280.423 kb on + strand, within PGA1_c21990 at 2280.543 kb on + strand, within PGA1_c21990 at 2280.661 kb on - strand at 2280.669 kb on + strand at 2280.677 kb on - strand at 2280.724 kb on + strand at 2280.839 kb on - strand at 2280.849 kb on - strand at 2280.911 kb on + strand, within PGA1_c22000 at 2280.919 kb on - strand, within PGA1_c22000 at 2280.937 kb on + strand, within PGA1_c22000 at 2280.987 kb on + strand, within PGA1_c22000 at 2281.002 kb on + strand, within PGA1_c22000 at 2281.010 kb on - strand, within PGA1_c22000 at 2281.055 kb on - strand, within PGA1_c22000 at 2281.055 kb on - strand, within PGA1_c22000 at 2281.091 kb on + strand, within PGA1_c22000 at 2281.091 kb on + strand, within PGA1_c22000 at 2281.091 kb on + strand, within PGA1_c22000 at 2281.091 kb on + strand, within PGA1_c22000 at 2281.099 kb on - strand, within PGA1_c22000 at 2281.099 kb on - strand, within PGA1_c22000 at 2281.099 kb on - strand, within PGA1_c22000 at 2281.115 kb on + strand, within PGA1_c22000 at 2281.273 kb on + strand, within PGA1_c22000 at 2281.273 kb on + strand, within PGA1_c22000 at 2281.273 kb on + strand, within PGA1_c22000 at 2281.273 kb on + strand, within PGA1_c22000 at 2281.273 kb on + strand, within PGA1_c22000 at 2281.273 kb on + strand, within PGA1_c22000 at 2281.273 kb on + strand, within PGA1_c22000 at 2281.273 kb on + strand, within PGA1_c22000 at 2281.273 kb on + strand, within PGA1_c22000 at 2281.273 kb on + strand, within PGA1_c22000 at 2281.281 kb on - strand, within PGA1_c22000 at 2281.281 kb on - strand, within PGA1_c22000 at 2281.281 kb on - strand, within PGA1_c22000 at 2281.281 kb on - strand, within PGA1_c22000 at 2281.281 kb on - strand, within PGA1_c22000 at 2281.281 kb on - strand, within PGA1_c22000 at 2281.281 kb on - strand, within PGA1_c22000 at 2281.298 kb on + strand, within PGA1_c22000 at 2281.372 kb on - strand, within PGA1_c22000 at 2281.475 kb on + strand at 2281.523 kb on - strand at 2281.523 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Nalidixic 0.016 mg/ml remove 2,279,879 - +0.5 2,279,906 + +0.4 2,279,906 + +1.2 2,279,912 + -0.5 2,279,912 + +0.9 2,279,912 + +0.4 2,279,912 + +0.6 2,279,920 - +1.0 2,279,920 - -0.2 2,279,938 + -1.3 2,279,938 + -0.4 2,279,986 + -2.0 2,280,053 + PGA1_c21990 0.12 +0.5 2,280,179 + PGA1_c21990 0.30 -1.3 2,280,214 - PGA1_c21990 0.35 -1.8 2,280,233 + PGA1_c21990 0.38 -0.2 2,280,312 - PGA1_c21990 0.50 -0.8 2,280,336 - PGA1_c21990 0.53 -1.1 2,280,338 - PGA1_c21990 0.53 -0.9 2,280,423 + PGA1_c21990 0.66 -0.3 2,280,543 + PGA1_c21990 0.83 +0.7 2,280,661 - +0.7 2,280,669 + +0.7 2,280,677 - +0.4 2,280,724 + -0.5 2,280,839 - -0.3 2,280,849 - +0.0 2,280,911 + PGA1_c22000 0.16 -0.0 2,280,919 - PGA1_c22000 0.17 -0.9 2,280,937 + PGA1_c22000 0.20 -0.1 2,280,987 + PGA1_c22000 0.28 -0.5 2,281,002 + PGA1_c22000 0.30 -0.8 2,281,010 - PGA1_c22000 0.31 -0.3 2,281,055 - PGA1_c22000 0.39 +0.8 2,281,055 - PGA1_c22000 0.39 +0.4 2,281,091 + PGA1_c22000 0.44 +0.0 2,281,091 + PGA1_c22000 0.44 +0.6 2,281,091 + PGA1_c22000 0.44 -0.0 2,281,091 + PGA1_c22000 0.44 -1.4 2,281,099 - PGA1_c22000 0.46 -1.0 2,281,099 - PGA1_c22000 0.46 -1.5 2,281,099 - PGA1_c22000 0.46 +0.1 2,281,115 + PGA1_c22000 0.48 +0.3 2,281,273 + PGA1_c22000 0.74 +0.3 2,281,273 + PGA1_c22000 0.74 -0.1 2,281,273 + PGA1_c22000 0.74 -0.1 2,281,273 + PGA1_c22000 0.74 -1.1 2,281,273 + PGA1_c22000 0.74 -0.4 2,281,273 + PGA1_c22000 0.74 +0.9 2,281,273 + PGA1_c22000 0.74 -0.3 2,281,273 + PGA1_c22000 0.74 -0.1 2,281,273 + PGA1_c22000 0.74 +1.0 2,281,273 + PGA1_c22000 0.74 +0.9 2,281,281 - PGA1_c22000 0.75 -0.9 2,281,281 - PGA1_c22000 0.75 -0.0 2,281,281 - PGA1_c22000 0.75 -1.3 2,281,281 - PGA1_c22000 0.75 -0.4 2,281,281 - PGA1_c22000 0.75 +0.3 2,281,281 - PGA1_c22000 0.75 +0.3 2,281,281 - PGA1_c22000 0.75 -1.1 2,281,298 + PGA1_c22000 0.78 -1.5 2,281,372 - PGA1_c22000 0.89 -0.1 2,281,475 + +1.4 2,281,523 - +0.2 2,281,523 - -2.0
Or see this region's nucleotide sequence