Experiment: SMN_rich_noKan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A3631 and Rru_A3632 are separated by 94 nucleotides Rru_A3632 and Rru_A3633 are separated by 51 nucleotides Rru_A3633 and Rru_A3634 are separated by 71 nucleotides Rru_A3634 and Rru_A3635 overlap by 4 nucleotides
Rru_A3631: Rru_A3631 - Leucyl-tRNA synthetase, class Ia (NCBI), at 4,171,032 to 4,173,620
_A3631
Rru_A3632: Rru_A3632 - hypothetical protein (NCBI), at 4,173,715 to 4,174,320
_A3632
Rru_A3633: Rru_A3633 - Thiamine monophosphate synthase (NCBI), at 4,174,372 to 4,175,190
_A3633
Rru_A3634: Rru_A3634 - Protein of unknown function UPF0001 (NCBI), at 4,175,262 to 4,175,990
_A3634
Rru_A3635: Rru_A3635 - hypothetical protein (NCBI), at 4,175,987 to 4,176,490
_A3635
Position (kb) 4174
4175
4176 Strain fitness (log2 ratio) -1
0
1 at 4173.602 kb on - strand at 4174.397 kb on - strand at 4175.098 kb on + strand, within Rru_A3633 at 4175.099 kb on - strand, within Rru_A3633 at 4175.099 kb on - strand, within Rru_A3633 at 4175.137 kb on + strand at 4175.137 kb on + strand at 4175.154 kb on + strand at 4175.154 kb on + strand at 4175.166 kb on + strand at 4175.684 kb on + strand, within Rru_A3634 at 4176.149 kb on - strand, within Rru_A3635
Per-strain Table
Position Strand Gene LocusTag Fraction SMN_rich_noKan remove 4,173,602 - +0.2 4,174,397 - +0.2 4,175,098 + Rru_A3633 0.89 +0.5 4,175,099 - Rru_A3633 0.89 +0.0 4,175,099 - Rru_A3633 0.89 +0.9 4,175,137 + -0.1 4,175,137 + +0.6 4,175,154 + +0.6 4,175,154 + -0.1 4,175,166 + +0.4 4,175,684 + Rru_A3634 0.58 +0.2 4,176,149 - Rru_A3635 0.32 +0.4
Or see this region's nucleotide sequence