Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_783 and Pf1N1B4_784 are separated by 108 nucleotides Pf1N1B4_784 and Pf1N1B4_785 are separated by 282 nucleotides Pf1N1B4_785 and Pf1N1B4_786 are separated by 1 nucleotides
Pf1N1B4_783 - 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase (EC 2.1.1.14), at 892,441 to 894,753
_783
Pf1N1B4_784 - Transcriptional activator MetR, at 894,862 to 895,779
_784
Pf1N1B4_785 - acetyltransferase, GNAT family, at 896,062 to 896,577
_785
Pf1N1B4_786 - L-lysine permease, at 896,579 to 897,217
_786
Position (kb) 894
895
896 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 893.879 kb on + strand, within Pf1N1B4_783 at 893.999 kb on + strand, within Pf1N1B4_783 at 894.000 kb on - strand, within Pf1N1B4_783 at 894.029 kb on + strand, within Pf1N1B4_783 at 894.044 kb on + strand, within Pf1N1B4_783 at 894.045 kb on - strand, within Pf1N1B4_783 at 894.230 kb on + strand, within Pf1N1B4_783 at 894.230 kb on + strand, within Pf1N1B4_783 at 894.230 kb on + strand, within Pf1N1B4_783 at 894.231 kb on - strand, within Pf1N1B4_783 at 894.231 kb on - strand, within Pf1N1B4_783 at 894.231 kb on - strand, within Pf1N1B4_783 at 894.231 kb on - strand, within Pf1N1B4_783 at 894.357 kb on - strand, within Pf1N1B4_783 at 894.357 kb on - strand, within Pf1N1B4_783 at 894.362 kb on + strand, within Pf1N1B4_783 at 894.362 kb on + strand, within Pf1N1B4_783 at 894.362 kb on + strand, within Pf1N1B4_783 at 894.362 kb on + strand, within Pf1N1B4_783 at 894.363 kb on - strand, within Pf1N1B4_783 at 894.363 kb on - strand at 894.363 kb on - strand, within Pf1N1B4_783 at 894.366 kb on + strand, within Pf1N1B4_783 at 894.366 kb on + strand, within Pf1N1B4_783 at 894.366 kb on + strand, within Pf1N1B4_783 at 894.411 kb on + strand, within Pf1N1B4_783 at 894.411 kb on + strand, within Pf1N1B4_783 at 894.412 kb on - strand, within Pf1N1B4_783 at 894.412 kb on - strand, within Pf1N1B4_783 at 894.547 kb on + strand at 894.547 kb on + strand at 894.770 kb on - strand at 894.771 kb on + strand at 894.772 kb on - strand at 894.784 kb on + strand at 894.785 kb on - strand at 894.785 kb on - strand at 894.785 kb on - strand at 894.785 kb on - strand at 894.785 kb on - strand at 895.333 kb on + strand, within Pf1N1B4_784 at 895.333 kb on + strand, within Pf1N1B4_784 at 895.333 kb on + strand, within Pf1N1B4_784 at 895.333 kb on + strand, within Pf1N1B4_784 at 895.333 kb on + strand, within Pf1N1B4_784 at 895.333 kb on + strand, within Pf1N1B4_784 at 895.333 kb on + strand, within Pf1N1B4_784 at 895.333 kb on + strand, within Pf1N1B4_784 at 895.333 kb on + strand, within Pf1N1B4_784 at 895.333 kb on + strand, within Pf1N1B4_784 at 895.333 kb on + strand, within Pf1N1B4_784 at 895.333 kb on + strand, within Pf1N1B4_784 at 895.333 kb on + strand, within Pf1N1B4_784 at 895.334 kb on - strand, within Pf1N1B4_784 at 895.334 kb on - strand, within Pf1N1B4_784 at 895.334 kb on - strand, within Pf1N1B4_784 at 895.334 kb on - strand, within Pf1N1B4_784 at 895.334 kb on - strand, within Pf1N1B4_784 at 895.334 kb on - strand, within Pf1N1B4_784 at 895.334 kb on - strand, within Pf1N1B4_784 at 895.334 kb on - strand, within Pf1N1B4_784 at 895.334 kb on - strand, within Pf1N1B4_784 at 895.334 kb on - strand, within Pf1N1B4_784 at 895.334 kb on - strand, within Pf1N1B4_784 at 895.334 kb on - strand, within Pf1N1B4_784 at 895.334 kb on - strand, within Pf1N1B4_784 at 895.351 kb on + strand, within Pf1N1B4_784 at 895.351 kb on + strand, within Pf1N1B4_784 at 895.351 kb on + strand, within Pf1N1B4_784 at 895.352 kb on - strand, within Pf1N1B4_784 at 895.352 kb on - strand, within Pf1N1B4_784 at 895.352 kb on - strand, within Pf1N1B4_784 at 895.402 kb on + strand, within Pf1N1B4_784 at 895.402 kb on + strand, within Pf1N1B4_784 at 895.402 kb on + strand, within Pf1N1B4_784 at 895.403 kb on - strand, within Pf1N1B4_784 at 895.403 kb on - strand, within Pf1N1B4_784 at 895.403 kb on - strand, within Pf1N1B4_784 at 895.606 kb on + strand, within Pf1N1B4_784 at 895.606 kb on + strand, within Pf1N1B4_784 at 895.606 kb on + strand at 895.606 kb on + strand, within Pf1N1B4_784 at 895.606 kb on + strand, within Pf1N1B4_784 at 895.606 kb on + strand, within Pf1N1B4_784 at 895.606 kb on + strand, within Pf1N1B4_784 at 895.607 kb on - strand, within Pf1N1B4_784 at 895.607 kb on - strand, within Pf1N1B4_784 at 895.607 kb on - strand, within Pf1N1B4_784 at 895.607 kb on - strand, within Pf1N1B4_784 at 895.607 kb on - strand, within Pf1N1B4_784 at 895.607 kb on - strand at 895.607 kb on - strand, within Pf1N1B4_784 at 895.607 kb on - strand, within Pf1N1B4_784 at 895.607 kb on - strand at 895.621 kb on + strand, within Pf1N1B4_784 at 895.621 kb on + strand, within Pf1N1B4_784 at 895.622 kb on - strand, within Pf1N1B4_784 at 895.670 kb on - strand, within Pf1N1B4_784 at 895.705 kb on + strand at 895.706 kb on - strand at 895.854 kb on + strand at 895.854 kb on + strand at 895.854 kb on + strand at 895.855 kb on - strand at 895.863 kb on + strand at 895.863 kb on + strand at 895.863 kb on + strand at 895.863 kb on + strand at 895.863 kb on + strand at 895.863 kb on + strand at 895.863 kb on + strand at 895.863 kb on + strand at 895.863 kb on + strand at 895.864 kb on - strand at 895.961 kb on + strand at 895.961 kb on + strand at 895.962 kb on - strand at 896.114 kb on + strand, within Pf1N1B4_785 at 896.115 kb on - strand, within Pf1N1B4_785 at 896.178 kb on - strand, within Pf1N1B4_785 at 896.178 kb on - strand, within Pf1N1B4_785 at 896.316 kb on - strand, within Pf1N1B4_785 at 896.316 kb on - strand, within Pf1N1B4_785 at 896.316 kb on - strand, within Pf1N1B4_785 at 896.416 kb on + strand, within Pf1N1B4_785 at 896.417 kb on - strand, within Pf1N1B4_785 at 896.417 kb on - strand, within Pf1N1B4_785 at 896.417 kb on - strand, within Pf1N1B4_785 at 896.646 kb on + strand, within Pf1N1B4_786 at 896.647 kb on - strand, within Pf1N1B4_786 at 896.647 kb on - strand, within Pf1N1B4_786
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 893,879 + Pf1N1B4_783 0.62 -0.0 893,999 + Pf1N1B4_783 0.67 -1.5 894,000 - Pf1N1B4_783 0.67 -0.0 894,029 + Pf1N1B4_783 0.69 -0.0 894,044 + Pf1N1B4_783 0.69 -1.6 894,045 - Pf1N1B4_783 0.69 -0.0 894,230 + Pf1N1B4_783 0.77 -0.5 894,230 + Pf1N1B4_783 0.77 -0.0 894,230 + Pf1N1B4_783 0.77 -1.5 894,231 - Pf1N1B4_783 0.77 -1.8 894,231 - Pf1N1B4_783 0.77 -0.0 894,231 - Pf1N1B4_783 0.77 -1.5 894,231 - Pf1N1B4_783 0.77 -4.1 894,357 - Pf1N1B4_783 0.83 -0.0 894,357 - Pf1N1B4_783 0.83 -1.8 894,362 + Pf1N1B4_783 0.83 -1.0 894,362 + Pf1N1B4_783 0.83 -0.0 894,362 + Pf1N1B4_783 0.83 -1.1 894,362 + Pf1N1B4_783 0.83 -1.4 894,363 - Pf1N1B4_783 0.83 -0.0 894,363 - -0.0 894,363 - Pf1N1B4_783 0.83 -0.0 894,366 + Pf1N1B4_783 0.83 -0.0 894,366 + Pf1N1B4_783 0.83 -0.0 894,366 + Pf1N1B4_783 0.83 -0.0 894,411 + Pf1N1B4_783 0.85 -0.0 894,411 + Pf1N1B4_783 0.85 -0.0 894,412 - Pf1N1B4_783 0.85 -0.0 894,412 - Pf1N1B4_783 0.85 -0.7 894,547 + -0.0 894,547 + -0.5 894,770 - -0.0 894,771 + -0.0 894,772 - -1.3 894,784 + -2.6 894,785 - -0.0 894,785 - -1.7 894,785 - -2.1 894,785 - -0.0 894,785 - -0.0 895,333 + Pf1N1B4_784 0.51 -0.0 895,333 + Pf1N1B4_784 0.51 -0.0 895,333 + Pf1N1B4_784 0.51 -0.0 895,333 + Pf1N1B4_784 0.51 -0.0 895,333 + Pf1N1B4_784 0.51 -0.0 895,333 + Pf1N1B4_784 0.51 -0.0 895,333 + Pf1N1B4_784 0.51 -1.4 895,333 + Pf1N1B4_784 0.51 -0.0 895,333 + Pf1N1B4_784 0.51 -0.0 895,333 + Pf1N1B4_784 0.51 -0.0 895,333 + Pf1N1B4_784 0.51 -0.0 895,333 + Pf1N1B4_784 0.51 -4.2 895,333 + Pf1N1B4_784 0.51 -0.0 895,334 - Pf1N1B4_784 0.51 -0.0 895,334 - Pf1N1B4_784 0.51 -0.0 895,334 - Pf1N1B4_784 0.51 -2.2 895,334 - Pf1N1B4_784 0.51 -3.9 895,334 - Pf1N1B4_784 0.51 -0.0 895,334 - Pf1N1B4_784 0.51 -0.0 895,334 - Pf1N1B4_784 0.51 -0.0 895,334 - Pf1N1B4_784 0.51 -0.0 895,334 - Pf1N1B4_784 0.51 -0.0 895,334 - Pf1N1B4_784 0.51 -1.9 895,334 - Pf1N1B4_784 0.51 -0.0 895,334 - Pf1N1B4_784 0.51 -2.4 895,334 - Pf1N1B4_784 0.51 -2.8 895,351 + Pf1N1B4_784 0.53 -0.0 895,351 + Pf1N1B4_784 0.53 -0.0 895,351 + Pf1N1B4_784 0.53 -2.6 895,352 - Pf1N1B4_784 0.53 -2.9 895,352 - Pf1N1B4_784 0.53 -0.0 895,352 - Pf1N1B4_784 0.53 -0.0 895,402 + Pf1N1B4_784 0.59 -0.0 895,402 + Pf1N1B4_784 0.59 -2.7 895,402 + Pf1N1B4_784 0.59 -0.0 895,403 - Pf1N1B4_784 0.59 -0.0 895,403 - Pf1N1B4_784 0.59 -0.0 895,403 - Pf1N1B4_784 0.59 -0.0 895,606 + Pf1N1B4_784 0.81 -0.0 895,606 + Pf1N1B4_784 0.81 -3.1 895,606 + -1.9 895,606 + Pf1N1B4_784 0.81 -0.0 895,606 + Pf1N1B4_784 0.81 -1.3 895,606 + Pf1N1B4_784 0.81 -0.0 895,606 + Pf1N1B4_784 0.81 -1.7 895,607 - Pf1N1B4_784 0.81 -2.6 895,607 - Pf1N1B4_784 0.81 -0.0 895,607 - Pf1N1B4_784 0.81 -0.0 895,607 - Pf1N1B4_784 0.81 -0.0 895,607 - Pf1N1B4_784 0.81 -2.7 895,607 - -0.0 895,607 - Pf1N1B4_784 0.81 -2.4 895,607 - Pf1N1B4_784 0.81 -0.0 895,607 - -0.0 895,621 + Pf1N1B4_784 0.83 -0.0 895,621 + Pf1N1B4_784 0.83 -0.0 895,622 - Pf1N1B4_784 0.83 -0.0 895,670 - Pf1N1B4_784 0.88 -0.0 895,705 + -0.0 895,706 - -2.7 895,854 + -0.0 895,854 + -0.0 895,854 + -0.0 895,855 - -0.0 895,863 + -0.0 895,863 + -1.8 895,863 + -0.0 895,863 + -1.6 895,863 + -1.7 895,863 + -0.0 895,863 + -0.0 895,863 + -1.5 895,863 + -0.0 895,864 - -1.4 895,961 + -0.0 895,961 + -0.0 895,962 - -0.0 896,114 + Pf1N1B4_785 0.10 -0.7 896,115 - Pf1N1B4_785 0.10 -0.0 896,178 - Pf1N1B4_785 0.22 -0.0 896,178 - Pf1N1B4_785 0.22 -0.0 896,316 - Pf1N1B4_785 0.49 -0.0 896,316 - Pf1N1B4_785 0.49 -0.0 896,316 - Pf1N1B4_785 0.49 -0.0 896,416 + Pf1N1B4_785 0.69 -2.3 896,417 - Pf1N1B4_785 0.69 -0.0 896,417 - Pf1N1B4_785 0.69 -2.4 896,417 - Pf1N1B4_785 0.69 -2.2 896,646 + Pf1N1B4_786 0.10 -2.0 896,647 - Pf1N1B4_786 0.11 -1.1 896,647 - Pf1N1B4_786 0.11 -0.0
Or see this region's nucleotide sequence