Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5249 and Pf1N1B4_5250 are separated by 353 nucleotides Pf1N1B4_5250 and Pf1N1B4_5251 are separated by 522 nucleotides
Pf1N1B4_5249 - Outer membrane protein A precursor, at 7,856 to 8,512
_5249
Pf1N1B4_5250 - hypothetical protein, at 8,866 to 8,994
_5250
Pf1N1B4_5251 - hypothetical protein, at 9,517 to 10,368
_5251
Position (kb)
8
9 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 7.926 kb on - strand, within Pf1N1B4_5249 at 7.986 kb on + strand, within Pf1N1B4_5249 at 7.993 kb on - strand, within Pf1N1B4_5249 at 8.219 kb on + strand, within Pf1N1B4_5249 at 8.219 kb on + strand, within Pf1N1B4_5249 at 8.220 kb on - strand, within Pf1N1B4_5249 at 8.220 kb on - strand, within Pf1N1B4_5249 at 8.220 kb on - strand, within Pf1N1B4_5249 at 8.228 kb on - strand, within Pf1N1B4_5249 at 8.289 kb on - strand, within Pf1N1B4_5249 at 8.343 kb on + strand, within Pf1N1B4_5249 at 8.428 kb on - strand, within Pf1N1B4_5249 at 8.594 kb on + strand at 8.595 kb on - strand at 8.595 kb on - strand at 8.642 kb on + strand at 8.643 kb on - strand at 8.654 kb on + strand at 8.655 kb on - strand at 8.659 kb on - strand at 8.659 kb on - strand at 8.659 kb on - strand at 8.659 kb on - strand at 8.659 kb on - strand at 8.676 kb on - strand at 8.761 kb on + strand at 8.761 kb on + strand at 8.814 kb on + strand at 8.818 kb on + strand at 8.819 kb on - strand at 8.819 kb on - strand at 9.008 kb on - strand at 9.074 kb on + strand at 9.074 kb on + strand at 9.075 kb on - strand at 9.406 kb on + strand at 9.406 kb on + strand at 9.406 kb on + strand at 9.406 kb on + strand at 9.406 kb on + strand at 9.407 kb on - strand at 9.407 kb on - strand at 9.407 kb on - strand at 9.407 kb on - strand at 9.410 kb on + strand at 9.410 kb on + strand at 9.411 kb on - strand at 9.411 kb on - strand at 9.417 kb on + strand at 9.417 kb on + strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.422 kb on - strand at 9.422 kb on - strand at 9.518 kb on + strand at 9.518 kb on + strand at 9.519 kb on - strand at 9.558 kb on + strand at 9.558 kb on + strand at 9.596 kb on + strand at 9.597 kb on - strand at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.712 kb on + strand, within Pf1N1B4_5251 at 9.920 kb on + strand at 9.920 kb on + strand, within Pf1N1B4_5251
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 7,926 - Pf1N1B4_5249 0.11 -1.2 7,986 + Pf1N1B4_5249 0.20 -0.0 7,993 - Pf1N1B4_5249 0.21 -0.0 8,219 + Pf1N1B4_5249 0.55 -0.0 8,219 + Pf1N1B4_5249 0.55 -0.0 8,220 - Pf1N1B4_5249 0.55 -1.3 8,220 - Pf1N1B4_5249 0.55 -0.0 8,220 - Pf1N1B4_5249 0.55 +0.3 8,228 - Pf1N1B4_5249 0.57 -0.0 8,289 - Pf1N1B4_5249 0.66 -0.0 8,343 + Pf1N1B4_5249 0.74 -0.0 8,428 - Pf1N1B4_5249 0.87 -0.0 8,594 + -0.7 8,595 - -0.0 8,595 - -0.0 8,642 + -0.0 8,643 - -1.6 8,654 + -0.0 8,655 - -0.0 8,659 - -0.0 8,659 - -0.0 8,659 - -0.0 8,659 - -0.0 8,659 - -0.0 8,676 - -0.0 8,761 + -0.0 8,761 + -0.0 8,814 + -0.0 8,818 + -0.0 8,819 - -2.7 8,819 - -2.9 9,008 - -2.2 9,074 + -1.0 9,074 + -1.9 9,075 - -0.0 9,406 + -0.0 9,406 + -0.0 9,406 + -0.0 9,406 + -0.0 9,406 + -0.0 9,407 - -3.3 9,407 - -0.0 9,407 - -0.0 9,407 - -0.0 9,410 + -0.0 9,410 + -0.0 9,411 - -4.2 9,411 - -0.0 9,417 + -1.4 9,417 + -1.1 9,418 - -1.5 9,418 - -0.0 9,418 - -1.4 9,418 - -0.0 9,418 - -0.0 9,418 - -3.0 9,418 - -0.0 9,418 - -0.0 9,418 - +1.8 9,418 - -0.0 9,418 - -2.5 9,422 - -3.3 9,422 - -1.6 9,518 + -0.0 9,518 + -0.0 9,519 - -0.0 9,558 + -0.0 9,558 + -1.4 9,596 + -0.0 9,597 - -0.0 9,640 - Pf1N1B4_5251 0.14 -0.0 9,640 - Pf1N1B4_5251 0.14 -2.1 9,640 - Pf1N1B4_5251 0.14 -1.4 9,640 - Pf1N1B4_5251 0.14 -2.8 9,640 - Pf1N1B4_5251 0.14 -0.0 9,640 - Pf1N1B4_5251 0.14 +0.4 9,712 + Pf1N1B4_5251 0.23 -2.6 9,920 + -0.0 9,920 + Pf1N1B4_5251 0.47 -0.0
Or see this region's nucleotide sequence