Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4257

Experiment: Nitrate reduction

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4256 and Pf1N1B4_4257 are separated by 224 nucleotidesPf1N1B4_4257 and Pf1N1B4_4258 are separated by 65 nucleotidesPf1N1B4_4258 and Pf1N1B4_4259 are separated by 160 nucleotides Pf1N1B4_4256 - Probable transmembrane protein, at 4,718,468 to 4,719,148 _4256 Pf1N1B4_4257 - L-serine dehydratase, (PLP)-dependent (EC 4.3.1.17) @ Threonine dehydratase biosynthetic (EC 4.3.1.19), at 4,719,373 to 4,720,305 _4257 Pf1N1B4_4258 - Ribonucleotide reductase, alpha subunit, at 4,720,371 to 4,720,610 _4258 Pf1N1B4_4259 - hypothetical protein, at 4,720,771 to 4,721,544 _4259 Position (kb) 4719 4720 4721Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 4718.453 kb on + strandat 4718.453 kb on + strandat 4718.454 kb on - strandat 4718.454 kb on - strandat 4718.473 kb on - strandat 4718.497 kb on + strandat 4718.498 kb on - strandat 4718.517 kb on + strandat 4718.518 kb on - strandat 4718.518 kb on - strandat 4718.541 kb on - strand, within Pf1N1B4_4256at 4718.664 kb on + strandat 4718.664 kb on + strand, within Pf1N1B4_4256at 4718.861 kb on - strand, within Pf1N1B4_4256at 4718.862 kb on + strand, within Pf1N1B4_4256at 4718.863 kb on - strand, within Pf1N1B4_4256at 4719.076 kb on - strand, within Pf1N1B4_4256at 4719.150 kb on + strandat 4719.150 kb on + strandat 4719.150 kb on + strandat 4719.150 kb on + strandat 4719.150 kb on + strandat 4719.150 kb on + strandat 4719.150 kb on + strandat 4719.150 kb on + strandat 4719.151 kb on - strandat 4719.151 kb on - strandat 4719.151 kb on - strandat 4719.151 kb on - strandat 4719.151 kb on - strandat 4719.151 kb on - strandat 4719.151 kb on - strandat 4719.225 kb on - strandat 4719.288 kb on + strandat 4719.288 kb on + strandat 4719.288 kb on + strandat 4719.289 kb on - strandat 4719.289 kb on - strandat 4719.289 kb on - strandat 4719.314 kb on + strandat 4719.314 kb on + strandat 4719.314 kb on + strandat 4719.314 kb on + strandat 4719.315 kb on - strandat 4719.315 kb on - strandat 4719.315 kb on - strandat 4719.745 kb on + strand, within Pf1N1B4_4257at 4719.745 kb on + strand, within Pf1N1B4_4257at 4719.745 kb on + strand, within Pf1N1B4_4257at 4719.746 kb on - strandat 4719.746 kb on - strand, within Pf1N1B4_4257at 4720.303 kb on + strandat 4720.317 kb on + strandat 4720.462 kb on + strand, within Pf1N1B4_4258at 4720.500 kb on - strand, within Pf1N1B4_4258at 4720.552 kb on + strand, within Pf1N1B4_4258at 4720.600 kb on - strandat 4720.638 kb on + strandat 4720.647 kb on + strandat 4720.647 kb on + strandat 4720.648 kb on - strandat 4720.656 kb on + strandat 4720.657 kb on - strandat 4720.698 kb on - strandat 4720.706 kb on + strandat 4720.706 kb on + strandat 4720.707 kb on - strandat 4720.707 kb on - strandat 4720.707 kb on - strandat 4720.707 kb on - strandat 4720.754 kb on + strandat 4720.754 kb on + strandat 4721.174 kb on - strand, within Pf1N1B4_4259at 4721.234 kb on - strand, within Pf1N1B4_4259

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction
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4,718,453 + -0.2
4,718,453 + -0.7
4,718,454 - -1.7
4,718,454 - -2.3
4,718,473 - -0.2
4,718,497 + -1.4
4,718,498 - -2.2
4,718,517 + -0.2
4,718,518 - -0.2
4,718,518 - -0.2
4,718,541 - Pf1N1B4_4256 0.11 -0.2
4,718,664 + -0.2
4,718,664 + Pf1N1B4_4256 0.29 -4.9
4,718,861 - Pf1N1B4_4256 0.58 -0.2
4,718,862 + Pf1N1B4_4256 0.58 -1.7
4,718,863 - Pf1N1B4_4256 0.58 -0.2
4,719,076 - Pf1N1B4_4256 0.89 -3.7
4,719,150 + -0.2
4,719,150 + -1.5
4,719,150 + -1.9
4,719,150 + -0.2
4,719,150 + -2.2
4,719,150 + -2.5
4,719,150 + -0.2
4,719,150 + -0.2
4,719,151 - -1.0
4,719,151 - -0.2
4,719,151 - -0.2
4,719,151 - -2.3
4,719,151 - -0.2
4,719,151 - -0.2
4,719,151 - -0.2
4,719,225 - -0.2
4,719,288 + -1.2
4,719,288 + -2.2
4,719,288 + -1.0
4,719,289 - -0.2
4,719,289 - -0.2
4,719,289 - -1.6
4,719,314 + -1.7
4,719,314 + -2.9
4,719,314 + -1.3
4,719,314 + -0.2
4,719,315 - -0.2
4,719,315 - -0.2
4,719,315 - -0.2
4,719,745 + Pf1N1B4_4257 0.40 -0.2
4,719,745 + Pf1N1B4_4257 0.40 -0.2
4,719,745 + Pf1N1B4_4257 0.40 -0.2
4,719,746 - -2.3
4,719,746 - Pf1N1B4_4257 0.40 -2.5
4,720,303 + -0.7
4,720,317 + -3.1
4,720,462 + Pf1N1B4_4258 0.38 -0.2
4,720,500 - Pf1N1B4_4258 0.54 -0.1
4,720,552 + Pf1N1B4_4258 0.75 -0.1
4,720,600 - -0.1
4,720,638 + -0.1
4,720,647 + -1.8
4,720,647 + -0.3
4,720,648 - -0.1
4,720,656 + -1.3
4,720,657 - -0.1
4,720,698 - -1.1
4,720,706 + -3.0
4,720,706 + -1.9
4,720,707 - -0.1
4,720,707 - -3.7
4,720,707 - -0.1
4,720,707 - -0.1
4,720,754 + -1.7
4,720,754 + -0.1
4,721,174 - Pf1N1B4_4259 0.52 -1.0
4,721,234 - Pf1N1B4_4259 0.60 -2.0

Or see this region's nucleotide sequence