Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4256 and Pf1N1B4_4257 are separated by 224 nucleotides Pf1N1B4_4257 and Pf1N1B4_4258 are separated by 65 nucleotides Pf1N1B4_4258 and Pf1N1B4_4259 are separated by 160 nucleotides
Pf1N1B4_4256 - Probable transmembrane protein, at 4,718,468 to 4,719,148
_4256
Pf1N1B4_4257 - L-serine dehydratase, (PLP)-dependent (EC 4.3.1.17) @ Threonine dehydratase biosynthetic (EC 4.3.1.19), at 4,719,373 to 4,720,305
_4257
Pf1N1B4_4258 - Ribonucleotide reductase, alpha subunit, at 4,720,371 to 4,720,610
_4258
Pf1N1B4_4259 - hypothetical protein, at 4,720,771 to 4,721,544
_4259
Position (kb)
4719
4720
4721 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4718.453 kb on + strand at 4718.453 kb on + strand at 4718.454 kb on - strand at 4718.454 kb on - strand at 4718.473 kb on - strand at 4718.497 kb on + strand at 4718.498 kb on - strand at 4718.517 kb on + strand at 4718.518 kb on - strand at 4718.518 kb on - strand at 4718.541 kb on - strand, within Pf1N1B4_4256 at 4718.664 kb on + strand at 4718.664 kb on + strand, within Pf1N1B4_4256 at 4718.861 kb on - strand, within Pf1N1B4_4256 at 4718.862 kb on + strand, within Pf1N1B4_4256 at 4718.863 kb on - strand, within Pf1N1B4_4256 at 4719.076 kb on - strand, within Pf1N1B4_4256 at 4719.150 kb on + strand at 4719.150 kb on + strand at 4719.150 kb on + strand at 4719.150 kb on + strand at 4719.150 kb on + strand at 4719.150 kb on + strand at 4719.150 kb on + strand at 4719.150 kb on + strand at 4719.151 kb on - strand at 4719.151 kb on - strand at 4719.151 kb on - strand at 4719.151 kb on - strand at 4719.151 kb on - strand at 4719.151 kb on - strand at 4719.151 kb on - strand at 4719.225 kb on - strand at 4719.288 kb on + strand at 4719.288 kb on + strand at 4719.288 kb on + strand at 4719.289 kb on - strand at 4719.289 kb on - strand at 4719.289 kb on - strand at 4719.314 kb on + strand at 4719.314 kb on + strand at 4719.314 kb on + strand at 4719.314 kb on + strand at 4719.315 kb on - strand at 4719.315 kb on - strand at 4719.315 kb on - strand at 4719.745 kb on + strand, within Pf1N1B4_4257 at 4719.745 kb on + strand, within Pf1N1B4_4257 at 4719.745 kb on + strand, within Pf1N1B4_4257 at 4719.746 kb on - strand at 4719.746 kb on - strand, within Pf1N1B4_4257 at 4720.303 kb on + strand at 4720.317 kb on + strand at 4720.462 kb on + strand, within Pf1N1B4_4258 at 4720.500 kb on - strand, within Pf1N1B4_4258 at 4720.552 kb on + strand, within Pf1N1B4_4258 at 4720.600 kb on - strand at 4720.638 kb on + strand at 4720.647 kb on + strand at 4720.647 kb on + strand at 4720.648 kb on - strand at 4720.656 kb on + strand at 4720.657 kb on - strand at 4720.698 kb on - strand at 4720.706 kb on + strand at 4720.706 kb on + strand at 4720.707 kb on - strand at 4720.707 kb on - strand at 4720.707 kb on - strand at 4720.707 kb on - strand at 4720.754 kb on + strand at 4720.754 kb on + strand at 4721.174 kb on - strand, within Pf1N1B4_4259 at 4721.234 kb on - strand, within Pf1N1B4_4259
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 4,718,453 + -0.2 4,718,453 + -0.7 4,718,454 - -1.7 4,718,454 - -2.3 4,718,473 - -0.2 4,718,497 + -1.4 4,718,498 - -2.2 4,718,517 + -0.2 4,718,518 - -0.2 4,718,518 - -0.2 4,718,541 - Pf1N1B4_4256 0.11 -0.2 4,718,664 + -0.2 4,718,664 + Pf1N1B4_4256 0.29 -4.9 4,718,861 - Pf1N1B4_4256 0.58 -0.2 4,718,862 + Pf1N1B4_4256 0.58 -1.7 4,718,863 - Pf1N1B4_4256 0.58 -0.2 4,719,076 - Pf1N1B4_4256 0.89 -3.7 4,719,150 + -0.2 4,719,150 + -1.5 4,719,150 + -1.9 4,719,150 + -0.2 4,719,150 + -2.2 4,719,150 + -2.5 4,719,150 + -0.2 4,719,150 + -0.2 4,719,151 - -1.0 4,719,151 - -0.2 4,719,151 - -0.2 4,719,151 - -2.3 4,719,151 - -0.2 4,719,151 - -0.2 4,719,151 - -0.2 4,719,225 - -0.2 4,719,288 + -1.2 4,719,288 + -2.2 4,719,288 + -1.0 4,719,289 - -0.2 4,719,289 - -0.2 4,719,289 - -1.6 4,719,314 + -1.7 4,719,314 + -2.9 4,719,314 + -1.3 4,719,314 + -0.2 4,719,315 - -0.2 4,719,315 - -0.2 4,719,315 - -0.2 4,719,745 + Pf1N1B4_4257 0.40 -0.2 4,719,745 + Pf1N1B4_4257 0.40 -0.2 4,719,745 + Pf1N1B4_4257 0.40 -0.2 4,719,746 - -2.3 4,719,746 - Pf1N1B4_4257 0.40 -2.5 4,720,303 + -0.7 4,720,317 + -3.1 4,720,462 + Pf1N1B4_4258 0.38 -0.2 4,720,500 - Pf1N1B4_4258 0.54 -0.1 4,720,552 + Pf1N1B4_4258 0.75 -0.1 4,720,600 - -0.1 4,720,638 + -0.1 4,720,647 + -1.8 4,720,647 + -0.3 4,720,648 - -0.1 4,720,656 + -1.3 4,720,657 - -0.1 4,720,698 - -1.1 4,720,706 + -3.0 4,720,706 + -1.9 4,720,707 - -0.1 4,720,707 - -3.7 4,720,707 - -0.1 4,720,707 - -0.1 4,720,754 + -1.7 4,720,754 + -0.1 4,721,174 - Pf1N1B4_4259 0.52 -1.0 4,721,234 - Pf1N1B4_4259 0.60 -2.0
Or see this region's nucleotide sequence