Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2290

Experiment: Nitrate reduction

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2289 and Pf1N1B4_2290 are separated by 127 nucleotidesPf1N1B4_2290 and Pf1N1B4_2291 are separated by 84 nucleotides Pf1N1B4_2289 - Thymidylate synthase (EC 2.1.1.45), at 2,604,895 to 2,605,866 _2289 Pf1N1B4_2290 - Cd(II)/Pb(II)-responsive transcriptional regulator, at 2,605,994 to 2,606,443 _2290 Pf1N1B4_2291 - Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4), at 2,606,528 to 2,608,786 _2291 Position (kb) 2605 2606 2607Strain fitness (log2 ratio) -3 -2 -1 0 1at 2605.882 kb on - strandat 2605.897 kb on - strandat 2605.966 kb on - strandat 2605.995 kb on + strandat 2605.995 kb on + strandat 2605.996 kb on - strandat 2605.996 kb on - strandat 2606.328 kb on + strand, within Pf1N1B4_2290at 2606.328 kb on + strand, within Pf1N1B4_2290at 2606.328 kb on + strand, within Pf1N1B4_2290at 2606.329 kb on - strand, within Pf1N1B4_2290at 2606.329 kb on - strand, within Pf1N1B4_2290at 2606.329 kb on - strand, within Pf1N1B4_2290at 2606.385 kb on + strand, within Pf1N1B4_2290at 2606.386 kb on - strand, within Pf1N1B4_2290at 2606.386 kb on - strand, within Pf1N1B4_2290at 2606.475 kb on - strandat 2606.494 kb on + strandat 2606.494 kb on + strandat 2606.495 kb on - strandat 2606.495 kb on - strandat 2606.495 kb on - strandat 2606.983 kb on + strand, within Pf1N1B4_2291at 2606.984 kb on - strand, within Pf1N1B4_2291at 2607.065 kb on + strand, within Pf1N1B4_2291at 2607.065 kb on + strand, within Pf1N1B4_2291at 2607.065 kb on + strand, within Pf1N1B4_2291at 2607.065 kb on + strand, within Pf1N1B4_2291at 2607.065 kb on + strand, within Pf1N1B4_2291at 2607.065 kb on + strand, within Pf1N1B4_2291at 2607.066 kb on - strand, within Pf1N1B4_2291at 2607.387 kb on + strand, within Pf1N1B4_2291at 2607.387 kb on + strand, within Pf1N1B4_2291at 2607.387 kb on + strand, within Pf1N1B4_2291at 2607.388 kb on - strand, within Pf1N1B4_2291at 2607.388 kb on - strand, within Pf1N1B4_2291at 2607.388 kb on - strand, within Pf1N1B4_2291at 2607.388 kb on - strand, within Pf1N1B4_2291

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction
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2,605,882 - +0.1
2,605,897 - -0.4
2,605,966 - +0.1
2,605,995 + -1.8
2,605,995 + +0.1
2,605,996 - +0.1
2,605,996 - +0.1
2,606,328 + Pf1N1B4_2290 0.74 +0.1
2,606,328 + Pf1N1B4_2290 0.74 -3.2
2,606,328 + Pf1N1B4_2290 0.74 +0.1
2,606,329 - Pf1N1B4_2290 0.74 -1.6
2,606,329 - Pf1N1B4_2290 0.74 +0.1
2,606,329 - Pf1N1B4_2290 0.74 +0.1
2,606,385 + Pf1N1B4_2290 0.87 +0.1
2,606,386 - Pf1N1B4_2290 0.87 +0.1
2,606,386 - Pf1N1B4_2290 0.87 -3.4
2,606,475 - +0.1
2,606,494 + +0.1
2,606,494 + -1.2
2,606,495 - +0.1
2,606,495 - +0.1
2,606,495 - +0.1
2,606,983 + Pf1N1B4_2291 0.20 +0.1
2,606,984 - Pf1N1B4_2291 0.20 +0.1
2,607,065 + Pf1N1B4_2291 0.24 +0.1
2,607,065 + Pf1N1B4_2291 0.24 +0.1
2,607,065 + Pf1N1B4_2291 0.24 -2.0
2,607,065 + Pf1N1B4_2291 0.24 +0.1
2,607,065 + Pf1N1B4_2291 0.24 +0.1
2,607,065 + Pf1N1B4_2291 0.24 +0.1
2,607,066 - Pf1N1B4_2291 0.24 +0.1
2,607,387 + Pf1N1B4_2291 0.38 -2.8
2,607,387 + Pf1N1B4_2291 0.38 +0.1
2,607,387 + Pf1N1B4_2291 0.38 +0.1
2,607,388 - Pf1N1B4_2291 0.38 -1.4
2,607,388 - Pf1N1B4_2291 0.38 +0.1
2,607,388 - Pf1N1B4_2291 0.38 +0.1
2,607,388 - Pf1N1B4_2291 0.38 +0.1

Or see this region's nucleotide sequence