Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1689 and Pf1N1B4_1690 are separated by 424 nucleotides Pf1N1B4_1690 and Pf1N1B4_1691 overlap by 21 nucleotides Pf1N1B4_1691 and Pf1N1B4_1692 are separated by 97 nucleotides
Pf1N1B4_1689 - High-affinity choline uptake protein BetT, at 1,935,154 to 1,937,109
_1689
Pf1N1B4_1690 - Serine acetyltransferase (EC 2.3.1.30), at 1,937,534 to 1,938,460
_1690
Pf1N1B4_1691 - hypothetical protein, at 1,938,440 to 1,938,553
_1691
Pf1N1B4_1692 - D-cysteine desulfhydrase (EC 4.4.1.15), at 1,938,651 to 1,939,643
_1692
Position (kb)
1937
1938
1939 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1936.641 kb on + strand, within Pf1N1B4_1689 at 1936.641 kb on + strand, within Pf1N1B4_1689 at 1936.642 kb on - strand, within Pf1N1B4_1689 at 1936.642 kb on - strand, within Pf1N1B4_1689 at 1936.658 kb on + strand, within Pf1N1B4_1689 at 1936.659 kb on - strand, within Pf1N1B4_1689 at 1936.869 kb on - strand, within Pf1N1B4_1689 at 1936.869 kb on - strand, within Pf1N1B4_1689 at 1936.952 kb on + strand at 1936.952 kb on + strand at 1936.952 kb on + strand at 1936.953 kb on - strand at 1936.953 kb on - strand at 1936.973 kb on + strand at 1936.973 kb on + strand at 1936.973 kb on + strand at 1936.973 kb on + strand at 1936.973 kb on + strand at 1936.973 kb on + strand at 1936.973 kb on + strand at 1936.973 kb on + strand at 1936.974 kb on - strand at 1936.974 kb on - strand at 1936.974 kb on - strand at 1936.974 kb on - strand at 1936.974 kb on - strand at 1936.974 kb on - strand at 1936.974 kb on - strand at 1936.976 kb on + strand at 1936.977 kb on - strand at 1936.977 kb on - strand at 1937.090 kb on + strand at 1937.090 kb on + strand at 1937.244 kb on - strand at 1937.265 kb on + strand at 1937.291 kb on - strand at 1937.309 kb on + strand at 1937.323 kb on + strand at 1937.323 kb on + strand at 1937.376 kb on + strand at 1937.380 kb on + strand at 1937.381 kb on - strand at 1937.381 kb on - strand at 1937.381 kb on - strand at 1937.458 kb on + strand at 1937.459 kb on - strand at 1937.459 kb on - strand at 1937.459 kb on - strand at 1937.535 kb on + strand at 1937.535 kb on + strand at 1937.536 kb on - strand at 1937.536 kb on - strand at 1937.536 kb on - strand at 1937.676 kb on + strand, within Pf1N1B4_1690 at 1937.676 kb on + strand, within Pf1N1B4_1690 at 1937.677 kb on - strand, within Pf1N1B4_1690 at 1937.677 kb on - strand, within Pf1N1B4_1690 at 1937.782 kb on - strand, within Pf1N1B4_1690 at 1937.782 kb on - strand, within Pf1N1B4_1690 at 1937.782 kb on - strand, within Pf1N1B4_1690 at 1938.056 kb on + strand, within Pf1N1B4_1690 at 1938.057 kb on - strand, within Pf1N1B4_1690 at 1938.237 kb on + strand, within Pf1N1B4_1690 at 1938.238 kb on - strand, within Pf1N1B4_1690 at 1938.238 kb on - strand, within Pf1N1B4_1690 at 1938.238 kb on - strand, within Pf1N1B4_1690 at 1938.512 kb on + strand, within Pf1N1B4_1691 at 1938.512 kb on + strand, within Pf1N1B4_1691 at 1938.512 kb on + strand, within Pf1N1B4_1691 at 1938.541 kb on + strand, within Pf1N1B4_1691 at 1938.572 kb on + strand at 1938.576 kb on + strand at 1938.601 kb on + strand at 1938.601 kb on + strand at 1938.602 kb on - strand at 1938.615 kb on + strand at 1938.615 kb on + strand at 1938.615 kb on + strand at 1938.615 kb on + strand at 1938.615 kb on + strand at 1938.615 kb on + strand at 1938.615 kb on + strand at 1938.616 kb on - strand at 1938.616 kb on - strand at 1938.616 kb on - strand at 1938.616 kb on - strand at 1938.617 kb on + strand at 1938.617 kb on + strand at 1938.756 kb on + strand, within Pf1N1B4_1692 at 1938.756 kb on + strand, within Pf1N1B4_1692 at 1938.756 kb on + strand, within Pf1N1B4_1692 at 1938.757 kb on - strand, within Pf1N1B4_1692 at 1938.757 kb on - strand at 1938.757 kb on - strand, within Pf1N1B4_1692 at 1938.757 kb on - strand, within Pf1N1B4_1692 at 1938.757 kb on - strand, within Pf1N1B4_1692 at 1938.757 kb on - strand at 1938.757 kb on - strand, within Pf1N1B4_1692 at 1938.757 kb on - strand, within Pf1N1B4_1692 at 1938.819 kb on + strand, within Pf1N1B4_1692 at 1938.854 kb on + strand, within Pf1N1B4_1692 at 1938.854 kb on + strand, within Pf1N1B4_1692 at 1938.854 kb on + strand, within Pf1N1B4_1692 at 1938.854 kb on + strand, within Pf1N1B4_1692 at 1938.854 kb on + strand, within Pf1N1B4_1692 at 1938.854 kb on + strand, within Pf1N1B4_1692 at 1938.855 kb on - strand, within Pf1N1B4_1692 at 1938.855 kb on - strand, within Pf1N1B4_1692 at 1938.978 kb on + strand, within Pf1N1B4_1692 at 1938.978 kb on + strand, within Pf1N1B4_1692 at 1938.978 kb on + strand, within Pf1N1B4_1692 at 1938.978 kb on + strand, within Pf1N1B4_1692 at 1938.978 kb on + strand, within Pf1N1B4_1692 at 1938.978 kb on + strand, within Pf1N1B4_1692 at 1938.978 kb on + strand, within Pf1N1B4_1692 at 1938.978 kb on + strand, within Pf1N1B4_1692 at 1938.978 kb on + strand, within Pf1N1B4_1692 at 1938.978 kb on + strand, within Pf1N1B4_1692 at 1938.978 kb on + strand, within Pf1N1B4_1692 at 1938.978 kb on + strand, within Pf1N1B4_1692 at 1938.979 kb on - strand, within Pf1N1B4_1692 at 1938.979 kb on - strand, within Pf1N1B4_1692 at 1938.979 kb on - strand, within Pf1N1B4_1692 at 1938.979 kb on - strand, within Pf1N1B4_1692 at 1938.979 kb on - strand, within Pf1N1B4_1692 at 1938.979 kb on - strand, within Pf1N1B4_1692 at 1938.979 kb on - strand, within Pf1N1B4_1692 at 1938.979 kb on - strand, within Pf1N1B4_1692 at 1938.979 kb on - strand, within Pf1N1B4_1692 at 1938.979 kb on - strand, within Pf1N1B4_1692 at 1938.979 kb on - strand, within Pf1N1B4_1692 at 1938.981 kb on - strand, within Pf1N1B4_1692 at 1939.110 kb on + strand, within Pf1N1B4_1692 at 1939.111 kb on - strand, within Pf1N1B4_1692 at 1939.111 kb on - strand, within Pf1N1B4_1692 at 1939.111 kb on - strand at 1939.155 kb on + strand, within Pf1N1B4_1692 at 1939.156 kb on - strand, within Pf1N1B4_1692 at 1939.156 kb on - strand, within Pf1N1B4_1692 at 1939.425 kb on + strand, within Pf1N1B4_1692 at 1939.425 kb on + strand, within Pf1N1B4_1692 at 1939.425 kb on + strand, within Pf1N1B4_1692 at 1939.425 kb on + strand, within Pf1N1B4_1692 at 1939.425 kb on + strand, within Pf1N1B4_1692 at 1939.425 kb on + strand, within Pf1N1B4_1692 at 1939.426 kb on - strand, within Pf1N1B4_1692 at 1939.426 kb on - strand, within Pf1N1B4_1692
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 1,936,641 + Pf1N1B4_1689 0.76 -2.5 1,936,641 + Pf1N1B4_1689 0.76 +0.0 1,936,642 - Pf1N1B4_1689 0.76 -2.8 1,936,642 - Pf1N1B4_1689 0.76 -2.8 1,936,658 + Pf1N1B4_1689 0.77 -1.8 1,936,659 - Pf1N1B4_1689 0.77 +0.0 1,936,869 - Pf1N1B4_1689 0.88 +0.0 1,936,869 - Pf1N1B4_1689 0.88 +0.0 1,936,952 + +0.2 1,936,952 + -2.1 1,936,952 + +0.0 1,936,953 - +0.0 1,936,953 - +0.0 1,936,973 + -0.7 1,936,973 + +0.0 1,936,973 + +0.0 1,936,973 + +0.0 1,936,973 + -2.2 1,936,973 + +0.0 1,936,973 + +0.0 1,936,973 + +0.0 1,936,974 - +0.0 1,936,974 - +0.0 1,936,974 - +0.0 1,936,974 - -0.8 1,936,974 - +0.0 1,936,974 - -3.2 1,936,974 - +0.0 1,936,976 + +0.0 1,936,977 - +0.0 1,936,977 - +0.0 1,937,090 + +0.0 1,937,090 + +0.0 1,937,244 - -1.2 1,937,265 + +0.0 1,937,291 - +0.0 1,937,309 + +0.0 1,937,323 + +0.0 1,937,323 + -1.9 1,937,376 + -0.2 1,937,380 + -1.6 1,937,381 - +0.0 1,937,381 - +0.0 1,937,381 - +0.0 1,937,458 + +0.0 1,937,459 - +0.0 1,937,459 - +0.0 1,937,459 - +0.0 1,937,535 + -1.0 1,937,535 + -1.0 1,937,536 - -2.9 1,937,536 - +0.0 1,937,536 - -1.4 1,937,676 + Pf1N1B4_1690 0.15 +0.0 1,937,676 + Pf1N1B4_1690 0.15 -0.1 1,937,677 - Pf1N1B4_1690 0.15 -1.7 1,937,677 - Pf1N1B4_1690 0.15 -0.6 1,937,782 - Pf1N1B4_1690 0.27 +0.0 1,937,782 - Pf1N1B4_1690 0.27 -3.0 1,937,782 - Pf1N1B4_1690 0.27 -0.8 1,938,056 + Pf1N1B4_1690 0.56 +0.0 1,938,057 - Pf1N1B4_1690 0.56 +0.0 1,938,237 + Pf1N1B4_1690 0.76 +0.0 1,938,238 - Pf1N1B4_1690 0.76 +0.0 1,938,238 - Pf1N1B4_1690 0.76 +0.0 1,938,238 - Pf1N1B4_1690 0.76 -1.1 1,938,512 + Pf1N1B4_1691 0.63 +0.0 1,938,512 + Pf1N1B4_1691 0.63 +0.0 1,938,512 + Pf1N1B4_1691 0.63 -3.8 1,938,541 + Pf1N1B4_1691 0.89 +0.0 1,938,572 + +0.0 1,938,576 + -1.6 1,938,601 + +0.0 1,938,601 + +0.0 1,938,602 - -2.4 1,938,615 + +0.0 1,938,615 + +0.0 1,938,615 + +0.0 1,938,615 + -1.6 1,938,615 + +0.0 1,938,615 + +0.0 1,938,615 + -2.8 1,938,616 - -1.6 1,938,616 - -4.5 1,938,616 - +0.0 1,938,616 - +0.0 1,938,617 + +0.0 1,938,617 + +0.0 1,938,756 + Pf1N1B4_1692 0.11 +0.0 1,938,756 + Pf1N1B4_1692 0.11 +0.0 1,938,756 + Pf1N1B4_1692 0.11 +0.0 1,938,757 - Pf1N1B4_1692 0.11 +0.0 1,938,757 - +0.0 1,938,757 - Pf1N1B4_1692 0.11 -1.0 1,938,757 - Pf1N1B4_1692 0.11 +0.0 1,938,757 - Pf1N1B4_1692 0.11 +0.0 1,938,757 - +0.0 1,938,757 - Pf1N1B4_1692 0.11 +0.0 1,938,757 - Pf1N1B4_1692 0.11 +0.0 1,938,819 + Pf1N1B4_1692 0.17 +0.0 1,938,854 + Pf1N1B4_1692 0.20 +0.0 1,938,854 + Pf1N1B4_1692 0.20 -2.5 1,938,854 + Pf1N1B4_1692 0.20 +0.0 1,938,854 + Pf1N1B4_1692 0.20 +0.0 1,938,854 + Pf1N1B4_1692 0.20 +0.0 1,938,854 + Pf1N1B4_1692 0.20 +0.0 1,938,855 - Pf1N1B4_1692 0.21 +0.5 1,938,855 - Pf1N1B4_1692 0.21 +0.0 1,938,978 + Pf1N1B4_1692 0.33 +0.0 1,938,978 + Pf1N1B4_1692 0.33 +0.0 1,938,978 + Pf1N1B4_1692 0.33 +0.0 1,938,978 + Pf1N1B4_1692 0.33 +0.0 1,938,978 + Pf1N1B4_1692 0.33 -2.9 1,938,978 + Pf1N1B4_1692 0.33 +0.0 1,938,978 + Pf1N1B4_1692 0.33 -2.2 1,938,978 + Pf1N1B4_1692 0.33 +0.0 1,938,978 + Pf1N1B4_1692 0.33 +0.0 1,938,978 + Pf1N1B4_1692 0.33 -0.8 1,938,978 + Pf1N1B4_1692 0.33 +0.0 1,938,978 + Pf1N1B4_1692 0.33 +0.0 1,938,979 - Pf1N1B4_1692 0.33 +0.0 1,938,979 - Pf1N1B4_1692 0.33 +0.0 1,938,979 - Pf1N1B4_1692 0.33 +0.0 1,938,979 - Pf1N1B4_1692 0.33 +0.0 1,938,979 - Pf1N1B4_1692 0.33 +0.0 1,938,979 - Pf1N1B4_1692 0.33 -2.8 1,938,979 - Pf1N1B4_1692 0.33 -3.7 1,938,979 - Pf1N1B4_1692 0.33 -1.8 1,938,979 - Pf1N1B4_1692 0.33 +0.0 1,938,979 - Pf1N1B4_1692 0.33 +0.0 1,938,979 - Pf1N1B4_1692 0.33 +0.0 1,938,981 - Pf1N1B4_1692 0.33 +0.0 1,939,110 + Pf1N1B4_1692 0.46 -2.5 1,939,111 - Pf1N1B4_1692 0.46 +0.0 1,939,111 - Pf1N1B4_1692 0.46 -3.5 1,939,111 - -3.8 1,939,155 + Pf1N1B4_1692 0.51 -1.8 1,939,156 - Pf1N1B4_1692 0.51 +0.0 1,939,156 - Pf1N1B4_1692 0.51 -0.6 1,939,425 + Pf1N1B4_1692 0.78 +0.0 1,939,425 + Pf1N1B4_1692 0.78 +0.0 1,939,425 + Pf1N1B4_1692 0.78 +0.0 1,939,425 + Pf1N1B4_1692 0.78 -1.6 1,939,425 + Pf1N1B4_1692 0.78 +0.0 1,939,425 + Pf1N1B4_1692 0.78 +0.0 1,939,426 - Pf1N1B4_1692 0.78 +0.0 1,939,426 - Pf1N1B4_1692 0.78 -0.2
Or see this region's nucleotide sequence