Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_138 and Pf1N1B4_139 overlap by 4 nucleotides Pf1N1B4_139 and Pf1N1B4_140 are separated by 43 nucleotides
Pf1N1B4_138 - Putative dipeptidase, pyoverdin biosynthesis PvdM, at 154,539 to 155,915
_138
Pf1N1B4_139 - Pyoverdin biosynthesis protein PvdN, putative aminotransferase, class V, at 155,912 to 157,198
_139
Pf1N1B4_140 - PvdO, pyoverdine responsive serine/threonine kinase (predicted by OlgaV), at 157,242 to 158,129
_140
Position (kb) 155
156
157
158 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 155.611 kb on - strand at 155.670 kb on + strand, within Pf1N1B4_138 at 155.671 kb on - strand, within Pf1N1B4_138 at 156.071 kb on + strand, within Pf1N1B4_139 at 156.072 kb on - strand, within Pf1N1B4_139 at 156.072 kb on - strand, within Pf1N1B4_139 at 156.360 kb on - strand, within Pf1N1B4_139 at 156.360 kb on - strand, within Pf1N1B4_139 at 156.360 kb on - strand, within Pf1N1B4_139 at 156.617 kb on + strand, within Pf1N1B4_139 at 156.618 kb on - strand, within Pf1N1B4_139 at 156.618 kb on - strand, within Pf1N1B4_139 at 157.242 kb on - strand at 157.242 kb on - strand at 157.542 kb on + strand, within Pf1N1B4_140 at 157.677 kb on + strand, within Pf1N1B4_140 at 157.677 kb on + strand, within Pf1N1B4_140 at 157.677 kb on + strand, within Pf1N1B4_140 at 157.677 kb on + strand, within Pf1N1B4_140 at 157.743 kb on + strand, within Pf1N1B4_140 at 157.744 kb on - strand at 157.831 kb on - strand, within Pf1N1B4_140 at 157.848 kb on + strand, within Pf1N1B4_140 at 157.921 kb on - strand, within Pf1N1B4_140 at 157.921 kb on - strand, within Pf1N1B4_140 at 157.953 kb on + strand, within Pf1N1B4_140 at 157.953 kb on + strand, within Pf1N1B4_140 at 157.954 kb on - strand, within Pf1N1B4_140 at 157.954 kb on - strand, within Pf1N1B4_140 at 157.954 kb on - strand, within Pf1N1B4_140 at 157.954 kb on - strand, within Pf1N1B4_140 at 158.113 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 155,611 - -0.7 155,670 + Pf1N1B4_138 0.82 -1.9 155,671 - Pf1N1B4_138 0.82 -0.0 156,071 + Pf1N1B4_139 0.12 +0.6 156,072 - Pf1N1B4_139 0.12 -0.0 156,072 - Pf1N1B4_139 0.12 -0.0 156,360 - Pf1N1B4_139 0.35 -0.0 156,360 - Pf1N1B4_139 0.35 -0.0 156,360 - Pf1N1B4_139 0.35 -0.0 156,617 + Pf1N1B4_139 0.55 -0.0 156,618 - Pf1N1B4_139 0.55 -0.0 156,618 - Pf1N1B4_139 0.55 -0.0 157,242 - -0.0 157,242 - -0.0 157,542 + Pf1N1B4_140 0.34 -0.0 157,677 + Pf1N1B4_140 0.49 -1.0 157,677 + Pf1N1B4_140 0.49 -0.0 157,677 + Pf1N1B4_140 0.49 -0.0 157,677 + Pf1N1B4_140 0.49 -0.0 157,743 + Pf1N1B4_140 0.56 -0.0 157,744 - -4.8 157,831 - Pf1N1B4_140 0.66 -0.0 157,848 + Pf1N1B4_140 0.68 -0.0 157,921 - Pf1N1B4_140 0.76 -0.0 157,921 - Pf1N1B4_140 0.76 -0.0 157,953 + Pf1N1B4_140 0.80 -1.4 157,953 + Pf1N1B4_140 0.80 -2.4 157,954 - Pf1N1B4_140 0.80 -0.0 157,954 - Pf1N1B4_140 0.80 -3.5 157,954 - Pf1N1B4_140 0.80 -0.0 157,954 - Pf1N1B4_140 0.80 -1.6 158,113 + -0.0
Or see this region's nucleotide sequence