Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1078 and Pf1N1B4_1079 overlap by 1 nucleotides Pf1N1B4_1079 and Pf1N1B4_1080 are separated by 209 nucleotides Pf1N1B4_1080 and Pf1N1B4_1081 are separated by 79 nucleotides Pf1N1B4_1081 and Pf1N1B4_1082 are separated by 54 nucleotides Pf1N1B4_1082 and Pf1N1B4_1083 are separated by 2 nucleotides
Pf1N1B4_1078 - LptA, protein essential for LPS transport across the periplasm, at 1,210,749 to 1,211,240
_1078
Pf1N1B4_1079 - Lipopolysaccharide ABC transporter, ATP-binding protein LptB, at 1,211,240 to 1,211,965
_1079
Pf1N1B4_1080 - RNA polymerase sigma-54 factor RpoN, at 1,212,175 to 1,213,644
_1080
Pf1N1B4_1081 - Ribosome hibernation protein YhbH, at 1,213,724 to 1,214,032
_1081
Pf1N1B4_1082 - PTS IIA-like nitrogen-regulatory protein PtsN, at 1,214,087 to 1,214,509
_1082
Pf1N1B4_1083 - Hypothetical ATP-binding protein UPF0042, contains P-loop, at 1,214,512 to 1,215,369
_1083
Position (kb) 1212
1213
1214 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 1211.964 kb on - strand at 1211.964 kb on - strand at 1211.964 kb on - strand at 1212.009 kb on - strand at 1212.027 kb on + strand at 1212.027 kb on + strand at 1212.028 kb on - strand at 1212.033 kb on + strand at 1212.033 kb on + strand at 1212.034 kb on - strand at 1212.044 kb on - strand at 1212.044 kb on - strand at 1212.044 kb on - strand at 1212.071 kb on - strand at 1212.071 kb on - strand at 1212.103 kb on + strand at 1212.131 kb on + strand at 1212.139 kb on + strand at 1213.696 kb on + strand at 1213.696 kb on + strand at 1213.723 kb on + strand at 1213.723 kb on + strand at 1213.723 kb on + strand at 1213.778 kb on + strand at 1213.778 kb on + strand, within Pf1N1B4_1081 at 1213.778 kb on + strand, within Pf1N1B4_1081 at 1213.888 kb on + strand, within Pf1N1B4_1081 at 1213.888 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand at 1213.934 kb on + strand at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand, within Pf1N1B4_1081 at 1213.934 kb on + strand at 1213.934 kb on + strand, within Pf1N1B4_1081
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 1,211,964 - -0.0 1,211,964 - -1.3 1,211,964 - -2.6 1,212,009 - -0.7 1,212,027 + -0.0 1,212,027 + -1.3 1,212,028 - -0.0 1,212,033 + -0.0 1,212,033 + -0.0 1,212,034 - -0.0 1,212,044 - -0.0 1,212,044 - -0.0 1,212,044 - -2.7 1,212,071 - -0.0 1,212,071 - -1.8 1,212,103 + -0.0 1,212,131 + -0.0 1,212,139 + -0.0 1,213,696 + -0.0 1,213,696 + -0.0 1,213,723 + -3.2 1,213,723 + -0.0 1,213,723 + -4.9 1,213,778 + -0.0 1,213,778 + Pf1N1B4_1081 0.17 -0.0 1,213,778 + Pf1N1B4_1081 0.17 -2.3 1,213,888 + Pf1N1B4_1081 0.53 -0.0 1,213,888 + Pf1N1B4_1081 0.53 -0.0 1,213,934 + -0.0 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -4.7 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -3.1 1,213,934 + -5.4 1,213,934 + -0.0 1,213,934 + Pf1N1B4_1081 0.68 -3.9 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + Pf1N1B4_1081 0.68 -0.0 1,213,934 + -0.0 1,213,934 + Pf1N1B4_1081 0.68 -3.2
Or see this region's nucleotide sequence