Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_262p01200 and PGA1_262p01210 are separated by 4 nucleotides PGA1_262p01210 and PGA1_262p01220 are separated by 3 nucleotides
PGA1_262p01200: PGA1_262p01200 - putative protein NorD, at 131,338 to 133,275
_262p01200
PGA1_262p01210: PGA1_262p01210 - putative protein NorQ, at 133,280 to 134,074
_262p01210
PGA1_262p01220: PGA1_262p01220 - nitric oxide reductase subunit B, at 134,078 to 135,436
_262p01220
Position (kb) 133
134
135 Strain fitness (log2 ratio) -2
-1
0
1 at 132.450 kb on - strand, within PGA1_262p01200 at 132.824 kb on + strand, within PGA1_262p01200 at 132.879 kb on + strand, within PGA1_262p01200 at 132.970 kb on + strand, within PGA1_262p01200 at 133.046 kb on - strand, within PGA1_262p01200 at 133.290 kb on - strand at 133.407 kb on + strand, within PGA1_262p01210 at 133.438 kb on + strand, within PGA1_262p01210 at 133.507 kb on + strand, within PGA1_262p01210 at 133.607 kb on - strand, within PGA1_262p01210 at 133.618 kb on - strand, within PGA1_262p01210 at 133.796 kb on - strand, within PGA1_262p01210 at 133.821 kb on + strand, within PGA1_262p01210 at 133.982 kb on + strand, within PGA1_262p01210 at 133.988 kb on + strand, within PGA1_262p01210 at 134.107 kb on - strand at 134.108 kb on - strand at 134.239 kb on - strand, within PGA1_262p01220 at 134.374 kb on - strand, within PGA1_262p01220 at 134.433 kb on - strand, within PGA1_262p01220 at 134.479 kb on - strand, within PGA1_262p01220 at 134.566 kb on - strand, within PGA1_262p01220 at 134.657 kb on - strand, within PGA1_262p01220 at 134.813 kb on - strand, within PGA1_262p01220 at 134.879 kb on + strand, within PGA1_262p01220 at 134.951 kb on - strand, within PGA1_262p01220 at 134.971 kb on - strand, within PGA1_262p01220 at 135.018 kb on + strand, within PGA1_262p01220
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 132,450 - PGA1_262p01200 0.57 +0.9 132,824 + PGA1_262p01200 0.77 -0.4 132,879 + PGA1_262p01200 0.80 -0.0 132,970 + PGA1_262p01200 0.84 -0.7 133,046 - PGA1_262p01200 0.88 -1.9 133,290 - +1.1 133,407 + PGA1_262p01210 0.16 +0.1 133,438 + PGA1_262p01210 0.20 +0.2 133,507 + PGA1_262p01210 0.29 +0.2 133,607 - PGA1_262p01210 0.41 -1.5 133,618 - PGA1_262p01210 0.43 -1.2 133,796 - PGA1_262p01210 0.65 -0.3 133,821 + PGA1_262p01210 0.68 -0.1 133,982 + PGA1_262p01210 0.88 +0.7 133,988 + PGA1_262p01210 0.89 -0.3 134,107 - +0.8 134,108 - -0.5 134,239 - PGA1_262p01220 0.12 -1.4 134,374 - PGA1_262p01220 0.22 +0.4 134,433 - PGA1_262p01220 0.26 -0.1 134,479 - PGA1_262p01220 0.30 -0.3 134,566 - PGA1_262p01220 0.36 -1.5 134,657 - PGA1_262p01220 0.43 -0.0 134,813 - PGA1_262p01220 0.54 -0.6 134,879 + PGA1_262p01220 0.59 -0.8 134,951 - PGA1_262p01220 0.64 -0.2 134,971 - PGA1_262p01220 0.66 -0.8 135,018 + PGA1_262p01220 0.69 +0.3
Or see this region's nucleotide sequence