Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c32880 and PGA1_c32890 are separated by 90 nucleotides PGA1_c32890 and PGA1_c32900 are separated by 22 nucleotides PGA1_c32900 and PGA1_c32910 overlap by 1 nucleotides PGA1_c32910 and PGA1_c32920 are separated by 3 nucleotides
PGA1_c32880: PGA1_c32880 - putative ribosomal RNA small subunit methyltransferase B, at 3,449,245 to 3,450,510
_c32880
PGA1_c32890: PGA1_c32890 - Protein of unknown function (DUF1674)., at 3,450,601 to 3,450,795
_c32890
PGA1_c32900: PGA1_c32900 - Antibiotic biosynthesis monooxygenase., at 3,450,818 to 3,450,973
_c32900
PGA1_c32910: PGA1_c32910 - hypothetical protein, at 3,450,973 to 3,451,152
_c32910
PGA1_c32920: PGA1_c32920 - dihydrodipicolinate reductase DapB, at 3,451,156 to 3,451,977
_c32920
Position (kb)
3450
3451 Strain fitness (log2 ratio)
-1
0
1 at 3449.620 kb on + strand, within PGA1_c32880 at 3449.657 kb on - strand, within PGA1_c32880 at 3449.680 kb on + strand, within PGA1_c32880 at 3449.687 kb on - strand, within PGA1_c32880 at 3449.702 kb on - strand, within PGA1_c32880 at 3449.702 kb on - strand, within PGA1_c32880 at 3449.770 kb on + strand, within PGA1_c32880 at 3449.780 kb on + strand, within PGA1_c32880 at 3449.780 kb on + strand, within PGA1_c32880 at 3449.788 kb on - strand, within PGA1_c32880 at 3449.853 kb on + strand, within PGA1_c32880 at 3449.884 kb on - strand, within PGA1_c32880 at 3449.894 kb on + strand, within PGA1_c32880 at 3449.894 kb on + strand, within PGA1_c32880 at 3449.906 kb on + strand, within PGA1_c32880 at 3449.906 kb on + strand, within PGA1_c32880 at 3449.906 kb on + strand, within PGA1_c32880 at 3449.913 kb on - strand, within PGA1_c32880 at 3449.914 kb on - strand, within PGA1_c32880 at 3449.921 kb on - strand, within PGA1_c32880 at 3450.029 kb on + strand, within PGA1_c32880 at 3450.029 kb on + strand, within PGA1_c32880 at 3450.239 kb on + strand, within PGA1_c32880 at 3450.239 kb on + strand, within PGA1_c32880 at 3450.245 kb on + strand, within PGA1_c32880 at 3450.245 kb on + strand, within PGA1_c32880 at 3450.245 kb on + strand, within PGA1_c32880 at 3450.245 kb on + strand, within PGA1_c32880 at 3450.247 kb on - strand, within PGA1_c32880 at 3450.253 kb on - strand, within PGA1_c32880 at 3450.253 kb on - strand, within PGA1_c32880 at 3450.263 kb on - strand, within PGA1_c32880 at 3450.263 kb on - strand, within PGA1_c32880 at 3450.286 kb on - strand, within PGA1_c32880 at 3450.286 kb on - strand, within PGA1_c32880 at 3450.305 kb on + strand, within PGA1_c32880 at 3450.333 kb on - strand, within PGA1_c32880 at 3450.369 kb on + strand, within PGA1_c32880 at 3450.390 kb on + strand at 3450.390 kb on + strand at 3450.390 kb on + strand at 3450.457 kb on - strand at 3450.471 kb on + strand at 3450.471 kb on + strand at 3450.490 kb on + strand at 3450.508 kb on - strand at 3450.611 kb on - strand at 3450.801 kb on + strand at 3450.806 kb on + strand at 3450.806 kb on + strand at 3450.814 kb on - strand at 3450.814 kb on - strand at 3450.851 kb on - strand, within PGA1_c32900 at 3450.874 kb on - strand, within PGA1_c32900 at 3450.897 kb on + strand, within PGA1_c32900 at 3450.897 kb on + strand, within PGA1_c32900 at 3450.897 kb on + strand, within PGA1_c32900 at 3450.897 kb on - strand, within PGA1_c32900 at 3450.905 kb on - strand, within PGA1_c32900 at 3450.905 kb on - strand, within PGA1_c32900 at 3450.910 kb on - strand, within PGA1_c32900 at 3450.910 kb on - strand, within PGA1_c32900 at 3450.910 kb on - strand, within PGA1_c32900 at 3451.057 kb on - strand, within PGA1_c32910 at 3451.106 kb on + strand, within PGA1_c32910 at 3451.108 kb on + strand, within PGA1_c32910 at 3451.116 kb on - strand, within PGA1_c32910 at 3451.136 kb on - strand at 3451.159 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 3,449,620 + PGA1_c32880 0.30 -0.1 3,449,657 - PGA1_c32880 0.33 -0.2 3,449,680 + PGA1_c32880 0.34 +1.4 3,449,687 - PGA1_c32880 0.35 +1.6 3,449,702 - PGA1_c32880 0.36 +0.2 3,449,702 - PGA1_c32880 0.36 +0.1 3,449,770 + PGA1_c32880 0.41 +0.0 3,449,780 + PGA1_c32880 0.42 -0.2 3,449,780 + PGA1_c32880 0.42 +0.5 3,449,788 - PGA1_c32880 0.43 +0.8 3,449,853 + PGA1_c32880 0.48 +0.2 3,449,884 - PGA1_c32880 0.50 -1.7 3,449,894 + PGA1_c32880 0.51 +0.6 3,449,894 + PGA1_c32880 0.51 +1.0 3,449,906 + PGA1_c32880 0.52 -0.0 3,449,906 + PGA1_c32880 0.52 +0.5 3,449,906 + PGA1_c32880 0.52 +0.1 3,449,913 - PGA1_c32880 0.53 +1.0 3,449,914 - PGA1_c32880 0.53 +0.1 3,449,921 - PGA1_c32880 0.53 -0.8 3,450,029 + PGA1_c32880 0.62 -0.4 3,450,029 + PGA1_c32880 0.62 +0.1 3,450,239 + PGA1_c32880 0.79 -0.1 3,450,239 + PGA1_c32880 0.79 +0.2 3,450,245 + PGA1_c32880 0.79 -0.5 3,450,245 + PGA1_c32880 0.79 -0.2 3,450,245 + PGA1_c32880 0.79 -0.5 3,450,245 + PGA1_c32880 0.79 +0.6 3,450,247 - PGA1_c32880 0.79 +1.2 3,450,253 - PGA1_c32880 0.80 -0.2 3,450,253 - PGA1_c32880 0.80 +0.1 3,450,263 - PGA1_c32880 0.80 -0.2 3,450,263 - PGA1_c32880 0.80 +0.9 3,450,286 - PGA1_c32880 0.82 -0.5 3,450,286 - PGA1_c32880 0.82 +0.6 3,450,305 + PGA1_c32880 0.84 -0.3 3,450,333 - PGA1_c32880 0.86 +0.5 3,450,369 + PGA1_c32880 0.89 -0.3 3,450,390 + -0.6 3,450,390 + +0.9 3,450,390 + +0.5 3,450,457 - +0.5 3,450,471 + -0.4 3,450,471 + +0.3 3,450,490 + +0.4 3,450,508 - +1.2 3,450,611 - +1.3 3,450,801 + +0.0 3,450,806 + -0.6 3,450,806 + -0.3 3,450,814 - +0.2 3,450,814 - +0.6 3,450,851 - PGA1_c32900 0.21 -1.3 3,450,874 - PGA1_c32900 0.36 +0.7 3,450,897 + PGA1_c32900 0.51 +0.0 3,450,897 + PGA1_c32900 0.51 +0.2 3,450,897 + PGA1_c32900 0.51 -0.1 3,450,897 - PGA1_c32900 0.51 +0.5 3,450,905 - PGA1_c32900 0.56 +0.3 3,450,905 - PGA1_c32900 0.56 +0.1 3,450,910 - PGA1_c32900 0.59 -0.3 3,450,910 - PGA1_c32900 0.59 -0.1 3,450,910 - PGA1_c32900 0.59 +0.6 3,451,057 - PGA1_c32910 0.47 -0.8 3,451,106 + PGA1_c32910 0.74 -1.1 3,451,108 + PGA1_c32910 0.75 -1.5 3,451,116 - PGA1_c32910 0.79 -0.4 3,451,136 - +0.3 3,451,159 - -0.2
Or see this region's nucleotide sequence