Experiment: marine broth
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_rRNA7 and HP15_tRNA20 are separated by 92 nucleotides HP15_tRNA20 and HP15_tRNA21 are separated by 122 nucleotides HP15_tRNA21 and HP15_rRNA8 are separated by 340 nucleotides HP15_rRNA8 and HP15_rRNA9 are separated by 101 nucleotides HP15_rRNA9 and HP15_1247 are separated by 66 nucleotides HP15_1247 and HP15_1248 overlap by 4 nucleotides
HP15_rRNA7: HP15_rRNA7 - 16S ribosomal RNA, at 1,298,821 to 1,300,352
_rRNA7
HP15_tRNA20: HP15_tRNA20 - tRNA-Ile, at 1,300,445 to 1,300,521
_tRNA20
HP15_tRNA21: HP15_tRNA21 - tRNA-Ala, at 1,300,644 to 1,300,719
_tRNA21
HP15_rRNA8: HP15_rRNA8 - 23S ribosomal RNA, at 1,301,060 to 1,303,951
_rRNA8
HP15_rRNA9: HP15_rRNA9 - 5S ribosomal RNA, at 1,304,053 to 1,304,166
_rRNA9
HP15_1247: HP15_1247 - signal transduction histidine kinase, at 1,304,233 to 1,304,913
_1247
HP15_1248: HP15_1248 - response regulator/TPR domain protein, at 1,304,910 to 1,306,547
_1248
Position (kb)
1301
1302
1303
1304 Strain fitness (log2 ratio)
-1
0
1 at 1304.231 kb on + strand at 1304.442 kb on + strand, within HP15_1247 at 1304.519 kb on - strand, within HP15_1247 at 1304.528 kb on + strand, within HP15_1247 at 1304.627 kb on - strand, within HP15_1247 at 1304.661 kb on + strand, within HP15_1247 at 1304.828 kb on + strand, within HP15_1247
Per-strain Table
Position Strand Gene LocusTag Fraction marine broth remove 1,304,231 + +0.6 1,304,442 + HP15_1247 0.31 +0.0 1,304,519 - HP15_1247 0.42 +0.3 1,304,528 + HP15_1247 0.43 +0.1 1,304,627 - HP15_1247 0.58 +0.2 1,304,661 + HP15_1247 0.63 -0.1 1,304,828 + HP15_1247 0.87 +1.1
Or see this region's nucleotide sequence