Experiment: MoLS4 with L-Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVUA0081 and xerD are separated by 304 nucleotides xerD and DVUA0084 are separated by 412 nucleotides DVUA0084 and DVUA0085 are separated by 54 nucleotides
DVUA0081: DVUA0081 - glycosyl transferase, group 1/2 family protein (TIGR), at 107,247 to 109,361
DVUA0081
DVUA0082: xerD - site-specific recombinase, phage integrase family (TIGR), at 109,666 to 110,697
xerD
DVUA0084: DVUA0084 - transcriptional regulator, AbrB family (TIGR), at 111,110 to 111,301
DVUA0084
DVUA0085: DVUA0085 - conserved hypothetical protein (TIGR), at 111,356 to 114,886
DVUA0085
Position (kb)
109
110
111 Strain fitness (log2 ratio)
-1
0
1
2 at 108.949 kb on + strand, within DVUA0081 at 109.181 kb on + strand at 109.189 kb on - strand at 109.189 kb on - strand at 109.376 kb on - strand at 109.376 kb on - strand at 109.376 kb on - strand at 109.382 kb on - strand at 109.388 kb on + strand at 109.440 kb on - strand at 109.871 kb on + strand, within xerD at 109.879 kb on - strand, within xerD at 109.913 kb on + strand, within xerD at 109.921 kb on - strand, within xerD at 109.991 kb on + strand, within xerD at 109.999 kb on - strand, within xerD at 109.999 kb on - strand, within xerD at 110.006 kb on + strand, within xerD at 110.014 kb on - strand, within xerD at 110.017 kb on - strand, within xerD at 110.311 kb on + strand, within xerD at 110.453 kb on - strand, within xerD at 110.494 kb on + strand, within xerD at 110.557 kb on + strand, within xerD at 110.721 kb on + strand at 110.729 kb on - strand at 110.743 kb on - strand at 110.743 kb on - strand at 110.763 kb on - strand at 110.765 kb on + strand at 110.803 kb on + strand at 111.038 kb on + strand at 111.075 kb on + strand at 111.254 kb on + strand, within DVUA0084 at 111.284 kb on - strand at 111.375 kb on + strand at 111.451 kb on + strand at 111.459 kb on - strand at 111.525 kb on - strand at 111.676 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glycine (N) remove 108,949 + DVUA0081 0.80 -0.5 109,181 + -0.3 109,189 - -0.3 109,189 - +2.3 109,376 - -1.1 109,376 - -0.2 109,376 - -0.9 109,382 - -0.9 109,388 + +1.2 109,440 - -0.5 109,871 + xerD DVUA0082 0.20 +0.5 109,879 - xerD DVUA0082 0.21 -0.6 109,913 + xerD DVUA0082 0.24 -0.9 109,921 - xerD DVUA0082 0.25 -1.5 109,991 + xerD DVUA0082 0.31 +0.5 109,999 - xerD DVUA0082 0.32 -0.3 109,999 - xerD DVUA0082 0.32 +0.2 110,006 + xerD DVUA0082 0.33 -0.4 110,014 - xerD DVUA0082 0.34 -0.8 110,017 - xerD DVUA0082 0.34 -0.6 110,311 + xerD DVUA0082 0.62 -0.1 110,453 - xerD DVUA0082 0.76 -0.4 110,494 + xerD DVUA0082 0.80 -0.3 110,557 + xerD DVUA0082 0.86 -0.6 110,721 + -0.2 110,729 - +1.0 110,743 - -0.1 110,743 - +0.6 110,763 - +0.2 110,765 + -0.2 110,803 + -1.6 111,038 + -0.4 111,075 + -0.3 111,254 + DVUA0084 0.75 -0.4 111,284 - -0.4 111,375 + -0.2 111,451 + -1.7 111,459 - +1.7 111,525 - -0.6 111,676 - -1.4
Or see this region's nucleotide sequence