Experiment: MoLS4 with L-Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rhlE and DVU1983 are separated by 517 nucleotides DVU1983 and msrA are separated by 127 nucleotides msrA and DVU1985 are separated by 109 nucleotides
DVU1982: rhlE - ATP-dependent RNA helicase RhlE (TIGR), at 2,058,879 to 2,060,267
rhlE
DVU1983: DVU1983 - hypothetical protein (TIGR), at 2,060,785 to 2,062,008
DVU1983
DVU1984: msrA - peptide methionine sulfoxide reductase MsrA (TIGR), at 2,062,136 to 2,062,762
msrA
DVU1985: DVU1985 - conserved domain protein (TIGR), at 2,062,872 to 2,064,128
DVU1985
Position (kb)
2060
2061
2062
2063 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2059.828 kb on - strand, within rhlE at 2059.927 kb on - strand, within rhlE at 2059.929 kb on - strand, within rhlE at 2060.074 kb on + strand, within rhlE at 2060.147 kb on - strand at 2060.191 kb on + strand at 2060.199 kb on - strand at 2060.199 kb on - strand at 2060.331 kb on + strand at 2060.359 kb on - strand at 2060.359 kb on - strand at 2060.378 kb on - strand at 2060.399 kb on + strand at 2060.407 kb on + strand at 2060.426 kb on - strand at 2060.488 kb on + strand at 2060.509 kb on - strand at 2060.509 kb on - strand at 2060.605 kb on - strand at 2060.843 kb on + strand at 2060.867 kb on + strand at 2060.867 kb on + strand at 2060.875 kb on - strand at 2060.875 kb on - strand at 2060.893 kb on + strand at 2061.000 kb on - strand, within DVU1983 at 2061.080 kb on - strand, within DVU1983 at 2061.142 kb on + strand, within DVU1983 at 2061.181 kb on - strand, within DVU1983 at 2061.294 kb on + strand, within DVU1983 at 2061.309 kb on + strand, within DVU1983 at 2061.332 kb on - strand, within DVU1983 at 2061.337 kb on - strand, within DVU1983 at 2061.395 kb on + strand, within DVU1983 at 2061.444 kb on + strand, within DVU1983 at 2061.463 kb on + strand, within DVU1983 at 2061.511 kb on + strand, within DVU1983 at 2061.612 kb on + strand, within DVU1983 at 2061.715 kb on + strand, within DVU1983 at 2061.717 kb on + strand, within DVU1983 at 2061.723 kb on - strand, within DVU1983 at 2061.762 kb on + strand, within DVU1983 at 2061.764 kb on + strand, within DVU1983 at 2061.764 kb on + strand, within DVU1983 at 2061.764 kb on + strand, within DVU1983 at 2061.770 kb on - strand, within DVU1983 at 2061.772 kb on - strand, within DVU1983 at 2061.772 kb on - strand, within DVU1983 at 2061.772 kb on - strand, within DVU1983 at 2061.772 kb on - strand, within DVU1983 at 2061.772 kb on - strand, within DVU1983 at 2061.796 kb on + strand, within DVU1983 at 2061.808 kb on + strand, within DVU1983 at 2061.810 kb on + strand, within DVU1983 at 2061.810 kb on + strand, within DVU1983 at 2061.816 kb on - strand, within DVU1983 at 2061.816 kb on - strand, within DVU1983 at 2061.955 kb on + strand at 2061.995 kb on - strand at 2062.008 kb on + strand at 2062.081 kb on - strand at 2062.175 kb on - strand at 2062.325 kb on + strand, within msrA at 2062.423 kb on - strand, within msrA at 2062.583 kb on - strand, within msrA at 2062.592 kb on - strand, within msrA at 2062.639 kb on + strand, within msrA at 2062.661 kb on + strand, within msrA at 2062.666 kb on + strand, within msrA at 2062.742 kb on + strand at 2062.752 kb on + strand at 2062.752 kb on + strand at 2062.908 kb on + strand at 2062.928 kb on + strand at 2062.965 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glycine (N) remove 2,059,828 - rhlE DVU1982 0.68 -0.1 2,059,927 - rhlE DVU1982 0.75 -1.3 2,059,929 - rhlE DVU1982 0.76 -0.2 2,060,074 + rhlE DVU1982 0.86 -1.2 2,060,147 - -0.3 2,060,191 + -1.4 2,060,199 - -0.3 2,060,199 - -1.4 2,060,331 + -0.1 2,060,359 - +1.4 2,060,359 - -0.4 2,060,378 - -0.1 2,060,399 + +0.2 2,060,407 + -0.2 2,060,426 - -0.5 2,060,488 + -0.4 2,060,509 - -0.2 2,060,509 - -0.5 2,060,605 - -2.5 2,060,843 + -0.3 2,060,867 + -0.4 2,060,867 + +0.8 2,060,875 - -0.6 2,060,875 - +2.8 2,060,893 + -0.1 2,061,000 - DVU1983 0.18 +0.1 2,061,080 - DVU1983 0.24 -0.2 2,061,142 + DVU1983 0.29 -0.4 2,061,181 - DVU1983 0.32 -0.8 2,061,294 + DVU1983 0.42 -0.1 2,061,309 + DVU1983 0.43 -0.7 2,061,332 - DVU1983 0.45 +0.8 2,061,337 - DVU1983 0.45 -1.0 2,061,395 + DVU1983 0.50 +0.1 2,061,444 + DVU1983 0.54 -0.2 2,061,463 + DVU1983 0.55 -0.2 2,061,511 + DVU1983 0.59 +0.5 2,061,612 + DVU1983 0.68 +0.2 2,061,715 + DVU1983 0.76 +0.3 2,061,717 + DVU1983 0.76 +0.7 2,061,723 - DVU1983 0.77 -0.5 2,061,762 + DVU1983 0.80 +0.7 2,061,764 + DVU1983 0.80 +0.3 2,061,764 + DVU1983 0.80 -0.7 2,061,764 + DVU1983 0.80 +0.1 2,061,770 - DVU1983 0.80 +0.5 2,061,772 - DVU1983 0.81 +0.7 2,061,772 - DVU1983 0.81 +0.4 2,061,772 - DVU1983 0.81 +1.5 2,061,772 - DVU1983 0.81 +2.0 2,061,772 - DVU1983 0.81 -0.2 2,061,796 + DVU1983 0.83 -0.3 2,061,808 + DVU1983 0.84 -0.2 2,061,810 + DVU1983 0.84 +0.2 2,061,810 + DVU1983 0.84 -0.2 2,061,816 - DVU1983 0.84 +0.3 2,061,816 - DVU1983 0.84 +0.2 2,061,955 + +0.5 2,061,995 - -0.1 2,062,008 + -0.8 2,062,081 - -1.2 2,062,175 - -1.3 2,062,325 + msrA DVU1984 0.30 +1.8 2,062,423 - msrA DVU1984 0.46 +0.4 2,062,583 - msrA DVU1984 0.71 +0.2 2,062,592 - msrA DVU1984 0.73 -0.4 2,062,639 + msrA DVU1984 0.80 -0.5 2,062,661 + msrA DVU1984 0.84 +0.5 2,062,666 + msrA DVU1984 0.85 -0.1 2,062,742 + -0.4 2,062,752 + -0.1 2,062,752 + +0.5 2,062,908 + +1.0 2,062,928 + +0.8 2,062,965 + +1.2
Or see this region's nucleotide sequence