Experiment: MoLS4 with L-Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysD and DVU1567 are separated by 9 nucleotides DVU1567 and ftn are separated by 163 nucleotides ftn and porA are separated by 72 nucleotides porA and porB overlap by 4 nucleotides
DVU1566: cysD - phosphoadenosine phosphosulfate reductase, putative (TIGR), at 1,649,064 to 1,649,738
cysD
DVU1567: DVU1567 - hypothetical protein (TIGR), at 1,649,748 to 1,649,960
DVU1567
DVU1568: ftn - ferritin (TIGR), at 1,650,124 to 1,650,633
ftn
DVU1569: porA - pyruvate ferredoxin oxidoreductase, alpha subunit (TIGR), at 1,650,706 to 1,652,442
porA
DVU1570: porB - pyruvate ferredoxin oxidoreductase, beta subunit (TIGR), at 1,652,439 to 1,653,293
porB
Position (kb)
1650
1651
1652
1653 Strain fitness (log2 ratio)
-2
-1
0
1 at 1649.745 kb on + strand at 1649.745 kb on + strand at 1649.747 kb on + strand at 1649.753 kb on - strand at 1649.769 kb on - strand at 1649.883 kb on + strand, within DVU1567 at 1649.978 kb on - strand at 1649.978 kb on - strand at 1649.994 kb on + strand at 1650.002 kb on - strand at 1650.026 kb on - strand at 1650.060 kb on + strand at 1650.060 kb on + strand at 1650.068 kb on - strand at 1650.170 kb on - strand at 1650.328 kb on + strand, within ftn at 1650.338 kb on - strand, within ftn at 1650.338 kb on - strand, within ftn at 1650.383 kb on - strand, within ftn at 1650.386 kb on + strand, within ftn at 1650.408 kb on + strand, within ftn at 1650.512 kb on - strand, within ftn at 1650.512 kb on - strand, within ftn at 1650.517 kb on - strand, within ftn at 1650.578 kb on - strand, within ftn at 1650.595 kb on - strand at 1650.685 kb on + strand at 1650.685 kb on + strand at 1650.788 kb on + strand at 1650.788 kb on + strand at 1650.788 kb on + strand at 1650.788 kb on + strand at 1650.796 kb on - strand at 1650.796 kb on - strand at 1650.833 kb on - strand at 1650.852 kb on + strand at 1650.858 kb on + strand at 1650.898 kb on + strand, within porA at 1650.926 kb on + strand, within porA at 1650.991 kb on - strand, within porA at 1651.011 kb on + strand, within porA at 1651.186 kb on - strand, within porA at 1651.194 kb on - strand, within porA at 1651.291 kb on - strand, within porA at 1651.292 kb on - strand, within porA at 1651.298 kb on - strand, within porA at 1651.392 kb on + strand, within porA at 1651.400 kb on - strand, within porA at 1651.400 kb on - strand, within porA at 1651.490 kb on - strand, within porA at 1651.621 kb on + strand, within porA at 1651.725 kb on - strand, within porA at 1651.737 kb on + strand, within porA at 1652.199 kb on + strand, within porA at 1652.207 kb on - strand, within porA at 1652.207 kb on - strand, within porA at 1652.208 kb on + strand, within porA at 1652.249 kb on + strand, within porA at 1652.253 kb on - strand, within porA at 1652.276 kb on + strand at 1652.483 kb on - strand at 1652.484 kb on - strand at 1652.665 kb on + strand, within porB at 1652.665 kb on + strand, within porB at 1652.671 kb on - strand, within porB at 1652.825 kb on + strand, within porB at 1653.107 kb on - strand, within porB at 1653.164 kb on - strand, within porB at 1653.208 kb on - strand at 1653.433 kb on - strand at 1653.433 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glycine (N) remove 1,649,745 + +0.1 1,649,745 + +0.4 1,649,747 + -0.2 1,649,753 - -0.3 1,649,769 - +0.0 1,649,883 + DVU1567 0.63 +0.1 1,649,978 - -0.6 1,649,978 - +0.6 1,649,994 + +0.7 1,650,002 - -0.8 1,650,026 - -1.8 1,650,060 + -0.5 1,650,060 + -1.3 1,650,068 - -0.1 1,650,170 - -0.1 1,650,328 + ftn DVU1568 0.40 -0.2 1,650,338 - ftn DVU1568 0.42 -0.5 1,650,338 - ftn DVU1568 0.42 +0.2 1,650,383 - ftn DVU1568 0.51 +0.1 1,650,386 + ftn DVU1568 0.51 -0.1 1,650,408 + ftn DVU1568 0.56 -0.3 1,650,512 - ftn DVU1568 0.76 -0.4 1,650,512 - ftn DVU1568 0.76 +0.2 1,650,517 - ftn DVU1568 0.77 +0.1 1,650,578 - ftn DVU1568 0.89 -0.4 1,650,595 - +0.3 1,650,685 + -0.1 1,650,685 + -0.4 1,650,788 + -0.6 1,650,788 + -0.5 1,650,788 + -0.6 1,650,788 + -0.5 1,650,796 - -0.9 1,650,796 - -0.3 1,650,833 - +0.4 1,650,852 + -0.2 1,650,858 + -1.9 1,650,898 + porA DVU1569 0.11 +0.1 1,650,926 + porA DVU1569 0.13 +0.6 1,650,991 - porA DVU1569 0.16 -0.6 1,651,011 + porA DVU1569 0.18 +0.6 1,651,186 - porA DVU1569 0.28 -0.3 1,651,194 - porA DVU1569 0.28 +1.3 1,651,291 - porA DVU1569 0.34 -0.2 1,651,292 - porA DVU1569 0.34 +0.4 1,651,298 - porA DVU1569 0.34 -0.5 1,651,392 + porA DVU1569 0.39 -0.4 1,651,400 - porA DVU1569 0.40 -0.0 1,651,400 - porA DVU1569 0.40 +0.2 1,651,490 - porA DVU1569 0.45 -0.1 1,651,621 + porA DVU1569 0.53 +0.0 1,651,725 - porA DVU1569 0.59 -0.1 1,651,737 + porA DVU1569 0.59 -0.1 1,652,199 + porA DVU1569 0.86 -0.0 1,652,207 - porA DVU1569 0.86 +0.0 1,652,207 - porA DVU1569 0.86 -0.1 1,652,208 + porA DVU1569 0.86 +1.1 1,652,249 + porA DVU1569 0.89 +1.1 1,652,253 - porA DVU1569 0.89 -0.2 1,652,276 + -0.3 1,652,483 - +1.0 1,652,484 - +0.1 1,652,665 + porB DVU1570 0.26 -0.0 1,652,665 + porB DVU1570 0.26 -0.4 1,652,671 - porB DVU1570 0.27 -1.3 1,652,825 + porB DVU1570 0.45 -1.1 1,653,107 - porB DVU1570 0.78 -0.4 1,653,164 - porB DVU1570 0.85 +0.2 1,653,208 - -0.9 1,653,433 - +0.6 1,653,433 - -0.1
Or see this region's nucleotide sequence