Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_784 and Pf1N1B4_785 are separated by 282 nucleotides Pf1N1B4_785 and Pf1N1B4_786 are separated by 1 nucleotides Pf1N1B4_786 and Pf1N1B4_787 are separated by 23 nucleotides Pf1N1B4_787 and Pf1N1B4_788 are separated by 207 nucleotides
Pf1N1B4_784 - Transcriptional activator MetR, at 894,862 to 895,779
_784
Pf1N1B4_785 - acetyltransferase, GNAT family, at 896,062 to 896,577
_785
Pf1N1B4_786 - L-lysine permease, at 896,579 to 897,217
_786
Pf1N1B4_787 - Nudix hydrolase family protein PA3470, at 897,241 to 897,675
_787
Pf1N1B4_788 - Response regulator, at 897,883 to 899,241
_788
Position (kb)
896
897
898 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 895.606 kb on + strand, within Pf1N1B4_784 at 895.606 kb on + strand, within Pf1N1B4_784 at 895.606 kb on + strand at 895.606 kb on + strand, within Pf1N1B4_784 at 895.606 kb on + strand, within Pf1N1B4_784 at 895.606 kb on + strand, within Pf1N1B4_784 at 895.606 kb on + strand, within Pf1N1B4_784 at 895.607 kb on - strand, within Pf1N1B4_784 at 895.607 kb on - strand, within Pf1N1B4_784 at 895.607 kb on - strand, within Pf1N1B4_784 at 895.607 kb on - strand, within Pf1N1B4_784 at 895.607 kb on - strand, within Pf1N1B4_784 at 895.607 kb on - strand at 895.607 kb on - strand, within Pf1N1B4_784 at 895.607 kb on - strand, within Pf1N1B4_784 at 895.607 kb on - strand at 895.621 kb on + strand, within Pf1N1B4_784 at 895.621 kb on + strand, within Pf1N1B4_784 at 895.622 kb on - strand, within Pf1N1B4_784 at 895.670 kb on - strand, within Pf1N1B4_784 at 895.705 kb on + strand at 895.706 kb on - strand at 895.854 kb on + strand at 895.854 kb on + strand at 895.854 kb on + strand at 895.855 kb on - strand at 895.863 kb on + strand at 895.863 kb on + strand at 895.863 kb on + strand at 895.863 kb on + strand at 895.863 kb on + strand at 895.863 kb on + strand at 895.863 kb on + strand at 895.863 kb on + strand at 895.863 kb on + strand at 895.864 kb on - strand at 895.961 kb on + strand at 895.961 kb on + strand at 895.962 kb on - strand at 896.114 kb on + strand, within Pf1N1B4_785 at 896.115 kb on - strand, within Pf1N1B4_785 at 896.178 kb on - strand, within Pf1N1B4_785 at 896.178 kb on - strand, within Pf1N1B4_785 at 896.316 kb on - strand, within Pf1N1B4_785 at 896.316 kb on - strand, within Pf1N1B4_785 at 896.316 kb on - strand, within Pf1N1B4_785 at 896.416 kb on + strand, within Pf1N1B4_785 at 896.417 kb on - strand, within Pf1N1B4_785 at 896.417 kb on - strand, within Pf1N1B4_785 at 896.417 kb on - strand, within Pf1N1B4_785 at 896.646 kb on + strand, within Pf1N1B4_786 at 896.647 kb on - strand, within Pf1N1B4_786 at 896.647 kb on - strand, within Pf1N1B4_786 at 896.970 kb on + strand at 897.063 kb on - strand, within Pf1N1B4_786 at 897.063 kb on - strand, within Pf1N1B4_786 at 897.138 kb on + strand, within Pf1N1B4_786 at 897.138 kb on + strand, within Pf1N1B4_786 at 897.138 kb on + strand, within Pf1N1B4_786 at 897.139 kb on - strand, within Pf1N1B4_786 at 897.139 kb on - strand, within Pf1N1B4_786 at 897.139 kb on - strand, within Pf1N1B4_786 at 897.164 kb on + strand at 897.281 kb on - strand at 897.281 kb on - strand at 897.409 kb on + strand at 897.410 kb on - strand, within Pf1N1B4_787 at 897.410 kb on - strand, within Pf1N1B4_787 at 897.746 kb on + strand at 897.747 kb on - strand at 897.765 kb on - strand at 897.798 kb on + strand at 897.812 kb on - strand at 897.822 kb on - strand at 897.823 kb on + strand at 897.824 kb on - strand at 897.824 kb on - strand at 897.824 kb on - strand at 897.824 kb on - strand at 897.824 kb on - strand at 897.884 kb on + strand at 897.884 kb on + strand at 897.884 kb on + strand at 897.884 kb on + strand at 897.885 kb on - strand at 897.885 kb on - strand at 897.885 kb on - strand at 897.885 kb on - strand at 897.934 kb on + strand at 897.934 kb on + strand at 897.934 kb on + strand at 897.935 kb on - strand at 897.935 kb on - strand at 897.935 kb on - strand at 897.935 kb on - strand at 897.935 kb on - strand at 897.935 kb on - strand at 897.935 kb on - strand at 897.942 kb on - strand at 897.942 kb on - strand at 897.942 kb on - strand at 898.130 kb on + strand, within Pf1N1B4_788 at 898.130 kb on + strand, within Pf1N1B4_788 at 898.130 kb on + strand, within Pf1N1B4_788 at 898.131 kb on - strand, within Pf1N1B4_788 at 898.131 kb on - strand, within Pf1N1B4_788 at 898.131 kb on - strand, within Pf1N1B4_788 at 898.176 kb on - strand, within Pf1N1B4_788 at 898.191 kb on - strand, within Pf1N1B4_788 at 898.202 kb on + strand, within Pf1N1B4_788 at 898.202 kb on + strand, within Pf1N1B4_788 at 898.202 kb on + strand, within Pf1N1B4_788 at 898.202 kb on + strand, within Pf1N1B4_788 at 898.202 kb on + strand, within Pf1N1B4_788 at 898.202 kb on + strand at 898.203 kb on - strand, within Pf1N1B4_788 at 898.203 kb on - strand, within Pf1N1B4_788 at 898.203 kb on - strand, within Pf1N1B4_788
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 895,606 + Pf1N1B4_784 0.81 +0.0 895,606 + Pf1N1B4_784 0.81 -2.2 895,606 + -2.4 895,606 + Pf1N1B4_784 0.81 +0.0 895,606 + Pf1N1B4_784 0.81 -1.9 895,606 + Pf1N1B4_784 0.81 +0.0 895,606 + Pf1N1B4_784 0.81 -1.7 895,607 - Pf1N1B4_784 0.81 -2.8 895,607 - Pf1N1B4_784 0.81 +0.0 895,607 - Pf1N1B4_784 0.81 +0.0 895,607 - Pf1N1B4_784 0.81 +0.0 895,607 - Pf1N1B4_784 0.81 -3.7 895,607 - +0.0 895,607 - Pf1N1B4_784 0.81 -4.4 895,607 - Pf1N1B4_784 0.81 +0.0 895,607 - +0.0 895,621 + Pf1N1B4_784 0.83 +0.0 895,621 + Pf1N1B4_784 0.83 +0.0 895,622 - Pf1N1B4_784 0.83 +0.0 895,670 - Pf1N1B4_784 0.88 +0.0 895,705 + +0.0 895,706 - -1.8 895,854 + +0.0 895,854 + +0.0 895,854 + +0.0 895,855 - +0.0 895,863 + +0.0 895,863 + -1.6 895,863 + +0.0 895,863 + -2.0 895,863 + -2.3 895,863 + +0.0 895,863 + +0.0 895,863 + -2.1 895,863 + +0.0 895,864 - -4.4 895,961 + +0.0 895,961 + +0.0 895,962 - +0.0 896,114 + Pf1N1B4_785 0.10 -3.1 896,115 - Pf1N1B4_785 0.10 +0.0 896,178 - Pf1N1B4_785 0.22 +0.0 896,178 - Pf1N1B4_785 0.22 +0.0 896,316 - Pf1N1B4_785 0.49 +0.0 896,316 - Pf1N1B4_785 0.49 +0.0 896,316 - Pf1N1B4_785 0.49 +0.0 896,416 + Pf1N1B4_785 0.69 -1.9 896,417 - Pf1N1B4_785 0.69 +0.0 896,417 - Pf1N1B4_785 0.69 -2.0 896,417 - Pf1N1B4_785 0.69 -0.5 896,646 + Pf1N1B4_786 0.10 -3.0 896,647 - Pf1N1B4_786 0.11 -4.6 896,647 - Pf1N1B4_786 0.11 +0.0 896,970 + +0.0 897,063 - Pf1N1B4_786 0.76 +0.0 897,063 - Pf1N1B4_786 0.76 -2.6 897,138 + Pf1N1B4_786 0.87 +0.0 897,138 + Pf1N1B4_786 0.87 +0.0 897,138 + Pf1N1B4_786 0.87 +0.0 897,139 - Pf1N1B4_786 0.88 +0.0 897,139 - Pf1N1B4_786 0.88 -3.8 897,139 - Pf1N1B4_786 0.88 +0.0 897,164 + +0.0 897,281 - -1.7 897,281 - -2.6 897,409 + +0.0 897,410 - Pf1N1B4_787 0.39 -2.1 897,410 - Pf1N1B4_787 0.39 +0.0 897,746 + +0.0 897,747 - +0.0 897,765 - +0.0 897,798 + -2.6 897,812 - -2.1 897,822 - +0.0 897,823 + +0.0 897,824 - +0.0 897,824 - +0.0 897,824 - +0.0 897,824 - -3.9 897,824 - +0.0 897,884 + +0.0 897,884 + -1.4 897,884 + -0.4 897,884 + +0.0 897,885 - +0.0 897,885 - +0.0 897,885 - +0.0 897,885 - +0.0 897,934 + +0.0 897,934 + +0.0 897,934 + +0.0 897,935 - -1.6 897,935 - -1.6 897,935 - -1.6 897,935 - -3.5 897,935 - -1.8 897,935 - +0.0 897,935 - -1.7 897,942 - +0.0 897,942 - +0.0 897,942 - +0.0 898,130 + Pf1N1B4_788 0.18 +0.0 898,130 + Pf1N1B4_788 0.18 -1.3 898,130 + Pf1N1B4_788 0.18 +0.0 898,131 - Pf1N1B4_788 0.18 +0.0 898,131 - Pf1N1B4_788 0.18 +0.0 898,131 - Pf1N1B4_788 0.18 +0.0 898,176 - Pf1N1B4_788 0.22 -1.6 898,191 - Pf1N1B4_788 0.23 +0.0 898,202 + Pf1N1B4_788 0.23 +0.0 898,202 + Pf1N1B4_788 0.23 +0.0 898,202 + Pf1N1B4_788 0.23 +0.0 898,202 + Pf1N1B4_788 0.23 +0.0 898,202 + Pf1N1B4_788 0.23 +0.0 898,202 + +0.0 898,203 - Pf1N1B4_788 0.24 +0.0 898,203 - Pf1N1B4_788 0.24 +0.0 898,203 - Pf1N1B4_788 0.24 +0.0
Or see this region's nucleotide sequence