Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_758 and Pf1N1B4_759 are separated by 197 nucleotides Pf1N1B4_759 and Pf1N1B4_760 are separated by 97 nucleotides Pf1N1B4_760 and Pf1N1B4_761 are separated by 134 nucleotides Pf1N1B4_761 and Pf1N1B4_762 are separated by 403 nucleotides
Pf1N1B4_758 - Bacterioferritin, at 862,129 to 862,602
_758
Pf1N1B4_759 - Glutaredoxin-related protein, at 862,800 to 863,141
_759
Pf1N1B4_760 - Formate dehydrogenase-O, major subunit (EC 1.2.1.2), at 863,239 to 865,344
_760
Pf1N1B4_761 - FIG00954879: hypothetical protein, at 865,479 to 865,769
_761
Pf1N1B4_762 - Ornithine carbamoyltransferase (EC 2.1.3.3), at 866,173 to 867,096
_762
Position (kb)
863
864
865
866 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 862.261 kb on + strand, within Pf1N1B4_758 at 862.573 kb on + strand at 862.573 kb on + strand at 862.574 kb on - strand at 862.574 kb on - strand at 862.574 kb on - strand at 862.600 kb on + strand at 862.600 kb on + strand at 862.601 kb on - strand at 862.607 kb on + strand at 862.629 kb on + strand at 862.630 kb on - strand at 862.649 kb on + strand at 862.650 kb on - strand at 862.651 kb on + strand at 862.667 kb on + strand at 862.730 kb on + strand at 862.744 kb on + strand at 862.744 kb on + strand at 862.744 kb on + strand at 862.744 kb on + strand at 862.744 kb on + strand at 862.744 kb on + strand at 862.745 kb on - strand at 862.745 kb on - strand at 862.745 kb on - strand at 862.952 kb on - strand, within Pf1N1B4_759 at 863.000 kb on - strand, within Pf1N1B4_759 at 863.086 kb on + strand, within Pf1N1B4_759 at 863.087 kb on - strand, within Pf1N1B4_759 at 863.194 kb on + strand at 863.194 kb on + strand at 863.567 kb on + strand, within Pf1N1B4_760 at 863.567 kb on + strand, within Pf1N1B4_760 at 863.568 kb on - strand at 863.568 kb on - strand, within Pf1N1B4_760 at 863.568 kb on - strand, within Pf1N1B4_760 at 863.568 kb on - strand, within Pf1N1B4_760 at 863.568 kb on - strand, within Pf1N1B4_760 at 863.644 kb on + strand, within Pf1N1B4_760 at 863.645 kb on - strand, within Pf1N1B4_760 at 863.645 kb on - strand, within Pf1N1B4_760 at 863.816 kb on + strand, within Pf1N1B4_760 at 863.871 kb on + strand, within Pf1N1B4_760 at 864.018 kb on - strand, within Pf1N1B4_760 at 864.018 kb on - strand, within Pf1N1B4_760 at 864.018 kb on - strand, within Pf1N1B4_760 at 864.018 kb on - strand, within Pf1N1B4_760 at 864.065 kb on + strand at 864.086 kb on + strand, within Pf1N1B4_760 at 864.086 kb on + strand, within Pf1N1B4_760 at 864.086 kb on + strand, within Pf1N1B4_760 at 864.087 kb on - strand at 864.087 kb on - strand, within Pf1N1B4_760 at 864.087 kb on - strand, within Pf1N1B4_760 at 864.087 kb on - strand, within Pf1N1B4_760 at 864.087 kb on - strand, within Pf1N1B4_760 at 864.087 kb on - strand, within Pf1N1B4_760 at 864.087 kb on - strand, within Pf1N1B4_760 at 864.087 kb on - strand, within Pf1N1B4_760 at 864.453 kb on - strand, within Pf1N1B4_760 at 864.630 kb on + strand, within Pf1N1B4_760 at 864.630 kb on + strand, within Pf1N1B4_760 at 864.630 kb on + strand, within Pf1N1B4_760 at 864.631 kb on - strand, within Pf1N1B4_760 at 864.887 kb on + strand, within Pf1N1B4_760 at 864.887 kb on + strand, within Pf1N1B4_760 at 864.887 kb on + strand, within Pf1N1B4_760 at 864.887 kb on + strand, within Pf1N1B4_760 at 864.887 kb on + strand, within Pf1N1B4_760 at 864.887 kb on + strand, within Pf1N1B4_760 at 864.887 kb on + strand, within Pf1N1B4_760 at 864.887 kb on + strand, within Pf1N1B4_760 at 864.887 kb on + strand, within Pf1N1B4_760 at 864.888 kb on - strand, within Pf1N1B4_760 at 864.888 kb on - strand at 864.888 kb on - strand, within Pf1N1B4_760 at 864.888 kb on - strand, within Pf1N1B4_760 at 864.888 kb on - strand, within Pf1N1B4_760 at 864.974 kb on + strand, within Pf1N1B4_760 at 864.974 kb on + strand, within Pf1N1B4_760 at 864.975 kb on - strand, within Pf1N1B4_760 at 864.975 kb on - strand at 864.998 kb on + strand, within Pf1N1B4_760 at 864.998 kb on + strand, within Pf1N1B4_760 at 864.999 kb on - strand, within Pf1N1B4_760 at 865.026 kb on - strand, within Pf1N1B4_760 at 865.110 kb on + strand, within Pf1N1B4_760 at 865.111 kb on - strand, within Pf1N1B4_760 at 865.255 kb on + strand at 865.398 kb on - strand at 865.398 kb on - strand at 865.398 kb on - strand at 865.398 kb on - strand at 865.727 kb on + strand at 865.728 kb on - strand, within Pf1N1B4_761 at 865.738 kb on - strand, within Pf1N1B4_761 at 865.738 kb on - strand, within Pf1N1B4_761 at 865.738 kb on - strand, within Pf1N1B4_761 at 865.738 kb on - strand, within Pf1N1B4_761 at 865.747 kb on - strand at 865.801 kb on - strand at 865.801 kb on - strand at 865.801 kb on - strand at 865.801 kb on - strand at 865.801 kb on - strand at 865.801 kb on - strand at 865.804 kb on + strand at 865.804 kb on + strand at 865.805 kb on - strand at 865.805 kb on - strand at 865.805 kb on - strand at 865.805 kb on - strand at 865.941 kb on + strand at 865.941 kb on + strand at 865.941 kb on + strand at 865.942 kb on - strand at 865.951 kb on + strand at 866.052 kb on + strand at 866.098 kb on + strand at 866.128 kb on - strand at 866.143 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 862,261 + Pf1N1B4_758 0.28 +0.0 862,573 + +0.0 862,573 + +0.0 862,574 - -1.5 862,574 - -2.6 862,574 - +0.0 862,600 + -1.2 862,600 + +0.0 862,601 - +0.0 862,607 + -1.1 862,629 + +0.0 862,630 - +0.0 862,649 + +0.0 862,650 - +0.0 862,651 + +0.0 862,667 + +0.0 862,730 + -3.3 862,744 + -2.2 862,744 + +0.0 862,744 + +0.0 862,744 + -2.1 862,744 + -2.3 862,744 + +0.0 862,745 - -2.7 862,745 - -3.1 862,745 - -2.6 862,952 - Pf1N1B4_759 0.44 -2.0 863,000 - Pf1N1B4_759 0.58 -2.3 863,086 + Pf1N1B4_759 0.84 +0.0 863,087 - Pf1N1B4_759 0.84 +0.0 863,194 + +0.0 863,194 + +0.0 863,567 + Pf1N1B4_760 0.16 +0.0 863,567 + Pf1N1B4_760 0.16 +0.0 863,568 - -4.1 863,568 - Pf1N1B4_760 0.16 +0.0 863,568 - Pf1N1B4_760 0.16 -2.5 863,568 - Pf1N1B4_760 0.16 +0.0 863,568 - Pf1N1B4_760 0.16 -1.4 863,644 + Pf1N1B4_760 0.19 +0.0 863,645 - Pf1N1B4_760 0.19 -1.8 863,645 - Pf1N1B4_760 0.19 +0.0 863,816 + Pf1N1B4_760 0.27 +0.0 863,871 + Pf1N1B4_760 0.30 -2.6 864,018 - Pf1N1B4_760 0.37 +0.0 864,018 - Pf1N1B4_760 0.37 +0.0 864,018 - Pf1N1B4_760 0.37 +0.0 864,018 - Pf1N1B4_760 0.37 +0.0 864,065 + +0.0 864,086 + Pf1N1B4_760 0.40 -1.7 864,086 + Pf1N1B4_760 0.40 +0.0 864,086 + Pf1N1B4_760 0.40 -1.4 864,087 - -1.0 864,087 - Pf1N1B4_760 0.40 +0.0 864,087 - Pf1N1B4_760 0.40 +0.0 864,087 - Pf1N1B4_760 0.40 -2.4 864,087 - Pf1N1B4_760 0.40 +0.0 864,087 - Pf1N1B4_760 0.40 +0.0 864,087 - Pf1N1B4_760 0.40 -1.7 864,087 - Pf1N1B4_760 0.40 +0.0 864,453 - Pf1N1B4_760 0.58 -1.9 864,630 + Pf1N1B4_760 0.66 +0.0 864,630 + Pf1N1B4_760 0.66 +0.0 864,630 + Pf1N1B4_760 0.66 +0.0 864,631 - Pf1N1B4_760 0.66 -1.0 864,887 + Pf1N1B4_760 0.78 -1.6 864,887 + Pf1N1B4_760 0.78 +0.0 864,887 + Pf1N1B4_760 0.78 -2.0 864,887 + Pf1N1B4_760 0.78 -1.9 864,887 + Pf1N1B4_760 0.78 +0.0 864,887 + Pf1N1B4_760 0.78 -1.6 864,887 + Pf1N1B4_760 0.78 +0.0 864,887 + Pf1N1B4_760 0.78 +0.0 864,887 + Pf1N1B4_760 0.78 +0.0 864,888 - Pf1N1B4_760 0.78 +0.0 864,888 - +0.0 864,888 - Pf1N1B4_760 0.78 -2.0 864,888 - Pf1N1B4_760 0.78 +1.0 864,888 - Pf1N1B4_760 0.78 +0.0 864,974 + Pf1N1B4_760 0.82 -3.3 864,974 + Pf1N1B4_760 0.82 +0.0 864,975 - Pf1N1B4_760 0.82 +0.0 864,975 - +0.0 864,998 + Pf1N1B4_760 0.84 +0.0 864,998 + Pf1N1B4_760 0.84 +0.0 864,999 - Pf1N1B4_760 0.84 +0.0 865,026 - Pf1N1B4_760 0.85 +0.0 865,110 + Pf1N1B4_760 0.89 +0.0 865,111 - Pf1N1B4_760 0.89 +0.0 865,255 + +0.0 865,398 - +0.0 865,398 - -4.1 865,398 - -1.4 865,398 - +0.0 865,727 + +0.0 865,728 - Pf1N1B4_761 0.86 +0.0 865,738 - Pf1N1B4_761 0.89 +0.0 865,738 - Pf1N1B4_761 0.89 -0.4 865,738 - Pf1N1B4_761 0.89 -0.9 865,738 - Pf1N1B4_761 0.89 -1.7 865,747 - +0.0 865,801 - +0.0 865,801 - -1.3 865,801 - -3.6 865,801 - +0.0 865,801 - +0.0 865,801 - +0.0 865,804 + +0.0 865,804 + +0.0 865,805 - +0.0 865,805 - -1.4 865,805 - +0.0 865,805 - -0.9 865,941 + -1.5 865,941 + -2.3 865,941 + +0.0 865,942 - +0.0 865,951 + +0.0 866,052 + +0.0 866,098 + +0.0 866,128 - +0.0 866,143 + +0.0
Or see this region's nucleotide sequence