Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5780

Experiment: Nitrate reduction

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5778 and Pf1N1B4_5779 overlap by 4 nucleotidesPf1N1B4_5779 and Pf1N1B4_5780 overlap by 4 nucleotidesPf1N1B4_5780 and Pf1N1B4_5781 are separated by 27 nucleotides Pf1N1B4_5778 - Nitrous oxide reductase maturation transmembrane protein NosY, at 214,797 to 215,627 _5778 Pf1N1B4_5779 - Nitrous oxide reductase maturation protein NosF (ATPase), at 215,624 to 216,547 _5779 Pf1N1B4_5780 - Nitrous oxide reductase maturation protein NosD, at 216,544 to 217,836 _5780 Pf1N1B4_5781 - Nitrous-oxide reductase (EC 1.7.99.6), at 217,864 to 219,783 _5781 Position (kb) 216 217 218Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 215.668 kb on - strandat 215.668 kb on - strandat 215.676 kb on + strandat 215.735 kb on + strand, within Pf1N1B4_5779at 215.736 kb on - strand, within Pf1N1B4_5779at 215.987 kb on + strand, within Pf1N1B4_5779at 215.987 kb on + strandat 215.988 kb on - strand, within Pf1N1B4_5779at 215.988 kb on - strandat 216.291 kb on + strand, within Pf1N1B4_5779at 216.505 kb on - strandat 216.510 kb on + strandat 216.738 kb on - strandat 216.842 kb on + strandat 216.843 kb on - strand, within Pf1N1B4_5780at 216.975 kb on - strand, within Pf1N1B4_5780at 217.070 kb on + strand, within Pf1N1B4_5780at 217.079 kb on + strand, within Pf1N1B4_5780at 217.080 kb on - strand, within Pf1N1B4_5780at 217.220 kb on + strand, within Pf1N1B4_5780at 217.220 kb on + strand, within Pf1N1B4_5780at 217.220 kb on + strand, within Pf1N1B4_5780at 217.220 kb on + strand, within Pf1N1B4_5780at 217.220 kb on + strand, within Pf1N1B4_5780at 217.220 kb on + strand, within Pf1N1B4_5780at 217.221 kb on - strandat 217.289 kb on + strand, within Pf1N1B4_5780at 217.289 kb on + strand, within Pf1N1B4_5780at 217.349 kb on + strand, within Pf1N1B4_5780at 217.563 kb on + strand, within Pf1N1B4_5780at 217.934 kb on + strandat 217.934 kb on + strandat 217.972 kb on - strandat 217.972 kb on - strandat 217.972 kb on - strandat 218.118 kb on - strand, within Pf1N1B4_5781at 218.141 kb on + strand, within Pf1N1B4_5781at 218.141 kb on + strand, within Pf1N1B4_5781at 218.142 kb on - strandat 218.496 kb on - strand, within Pf1N1B4_5781at 218.522 kb on + strandat 218.522 kb on + strand, within Pf1N1B4_5781at 218.544 kb on - strand, within Pf1N1B4_5781at 218.544 kb on - strand, within Pf1N1B4_5781at 218.774 kb on + strand, within Pf1N1B4_5781at 218.774 kb on + strand, within Pf1N1B4_5781at 218.774 kb on + strandat 218.774 kb on + strand, within Pf1N1B4_5781at 218.775 kb on - strand, within Pf1N1B4_5781at 218.775 kb on - strand, within Pf1N1B4_5781at 218.775 kb on - strand, within Pf1N1B4_5781at 218.829 kb on - strand, within Pf1N1B4_5781

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction
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215,668 - -0.0
215,668 - -2.1
215,676 + -4.5
215,735 + Pf1N1B4_5779 0.12 -0.0
215,736 - Pf1N1B4_5779 0.12 -4.4
215,987 + Pf1N1B4_5779 0.39 -0.0
215,987 + -0.0
215,988 - Pf1N1B4_5779 0.39 -0.0
215,988 - -1.9
216,291 + Pf1N1B4_5779 0.72 -0.0
216,505 - -0.0
216,510 + -0.0
216,738 - -1.2
216,842 + -0.0
216,843 - Pf1N1B4_5780 0.23 -0.0
216,975 - Pf1N1B4_5780 0.33 -2.3
217,070 + Pf1N1B4_5780 0.41 -1.3
217,079 + Pf1N1B4_5780 0.41 -0.0
217,080 - Pf1N1B4_5780 0.41 -0.0
217,220 + Pf1N1B4_5780 0.52 -0.0
217,220 + Pf1N1B4_5780 0.52 -3.9
217,220 + Pf1N1B4_5780 0.52 -3.1
217,220 + Pf1N1B4_5780 0.52 -0.0
217,220 + Pf1N1B4_5780 0.52 -4.1
217,220 + Pf1N1B4_5780 0.52 -3.0
217,221 - -0.0
217,289 + Pf1N1B4_5780 0.58 -0.0
217,289 + Pf1N1B4_5780 0.58 -2.5
217,349 + Pf1N1B4_5780 0.62 -0.0
217,563 + Pf1N1B4_5780 0.79 -0.0
217,934 + -3.3
217,934 + -5.1
217,972 - -4.0
217,972 - -0.0
217,972 - -2.3
218,118 - Pf1N1B4_5781 0.13 -0.0
218,141 + Pf1N1B4_5781 0.14 -2.6
218,141 + Pf1N1B4_5781 0.14 -0.4
218,142 - -4.4
218,496 - Pf1N1B4_5781 0.33 -0.0
218,522 + -0.0
218,522 + Pf1N1B4_5781 0.34 -0.0
218,544 - Pf1N1B4_5781 0.35 -2.1
218,544 - Pf1N1B4_5781 0.35 -0.0
218,774 + Pf1N1B4_5781 0.47 -0.0
218,774 + Pf1N1B4_5781 0.47 -0.0
218,774 + -5.4
218,774 + Pf1N1B4_5781 0.47 -0.0
218,775 - Pf1N1B4_5781 0.47 -0.0
218,775 - Pf1N1B4_5781 0.47 -0.0
218,775 - Pf1N1B4_5781 0.47 -0.0
218,829 - Pf1N1B4_5781 0.50 -2.3

Or see this region's nucleotide sequence