Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5770 and Pf1N1B4_5771 overlap by 26 nucleotides Pf1N1B4_5771 and Pf1N1B4_5772 are separated by 198 nucleotides Pf1N1B4_5772 and Pf1N1B4_5773 are separated by 70 nucleotides Pf1N1B4_5773 and Pf1N1B4_5774 overlap by 4 nucleotides
Pf1N1B4_5770 - Heme d1 biosynthesis protein NirG, at 207,646 to 208,089
_5770
Pf1N1B4_5771 - Heme d1 biosynthesis protein NirH, at 208,064 to 208,579
_5771
Pf1N1B4_5772 - Heme d1 biosynthesis protein NirJ, at 208,778 to 209,956
_5772
Pf1N1B4_5773 - Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 210,027 to 210,866
_5773
Pf1N1B4_5774 - Nitrite reductase associated c-type cytochorome NirN, at 210,863 to 212,356
_5774
Position (kb) 208
209
210 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 207.929 kb on - strand, within Pf1N1B4_5770 at 207.929 kb on - strand, within Pf1N1B4_5770 at 208.060 kb on + strand at 208.060 kb on + strand at 208.061 kb on - strand at 208.076 kb on - strand at 208.284 kb on - strand, within Pf1N1B4_5771 at 208.548 kb on + strand at 208.618 kb on + strand at 208.665 kb on - strand at 208.732 kb on + strand at 208.957 kb on + strand, within Pf1N1B4_5772 at 208.958 kb on - strand, within Pf1N1B4_5772 at 209.166 kb on - strand, within Pf1N1B4_5772 at 209.373 kb on - strand, within Pf1N1B4_5772 at 209.373 kb on - strand, within Pf1N1B4_5772 at 209.391 kb on - strand, within Pf1N1B4_5772 at 209.681 kb on + strand, within Pf1N1B4_5772 at 209.681 kb on + strand, within Pf1N1B4_5772 at 209.681 kb on + strand, within Pf1N1B4_5772 at 210.378 kb on + strand, within Pf1N1B4_5773 at 210.579 kb on + strand, within Pf1N1B4_5773 at 210.580 kb on - strand, within Pf1N1B4_5773
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 207,929 - Pf1N1B4_5770 0.64 -0.0 207,929 - Pf1N1B4_5770 0.64 -0.0 208,060 + -4.0 208,060 + -2.5 208,061 - -3.0 208,076 - -0.0 208,284 - Pf1N1B4_5771 0.43 -0.0 208,548 + -2.0 208,618 + -0.0 208,665 - -3.3 208,732 + -1.7 208,957 + Pf1N1B4_5772 0.15 -3.0 208,958 - Pf1N1B4_5772 0.15 -0.0 209,166 - Pf1N1B4_5772 0.33 -0.0 209,373 - Pf1N1B4_5772 0.50 -2.6 209,373 - Pf1N1B4_5772 0.50 -0.0 209,391 - Pf1N1B4_5772 0.52 -0.0 209,681 + Pf1N1B4_5772 0.77 -0.0 209,681 + Pf1N1B4_5772 0.77 -4.3 209,681 + Pf1N1B4_5772 0.77 -2.9 210,378 + Pf1N1B4_5773 0.42 -0.0 210,579 + Pf1N1B4_5773 0.66 -1.1 210,580 - Pf1N1B4_5773 0.66 -0.0
Or see this region's nucleotide sequence