Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5726 and Pf1N1B4_5727 are separated by 15 nucleotides Pf1N1B4_5727 and Pf1N1B4_5728 are separated by 5 nucleotides Pf1N1B4_5728 and Pf1N1B4_5729 are separated by 32 nucleotides Pf1N1B4_5729 and Pf1N1B4_5730 are separated by 277 nucleotides
Pf1N1B4_5726 - Ferredoxin, at 165,339 to 166,667
_5726
Pf1N1B4_5727 - Ribonucleotide reductase of class III (anaerobic), activating protein (EC 1.97.1.4), at 166,683 to 167,369
_5727
Pf1N1B4_5728 - hypothetical protein, at 167,375 to 167,542
_5728
Pf1N1B4_5729 - Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2), at 167,575 to 169,590
_5729
Pf1N1B4_5730 - Molybdenum cofactor biosynthesis protein MoaA, at 169,868 to 170,860
_5730
Position (kb) 167
168
169
170 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1
2
3 at 166.906 kb on + strand, within Pf1N1B4_5727 at 167.781 kb on - strand, within Pf1N1B4_5729 at 167.781 kb on - strand, within Pf1N1B4_5729 at 167.781 kb on - strand at 167.781 kb on - strand, within Pf1N1B4_5729 at 167.891 kb on + strand, within Pf1N1B4_5729 at 167.891 kb on + strand, within Pf1N1B4_5729 at 167.891 kb on + strand, within Pf1N1B4_5729 at 167.894 kb on + strand, within Pf1N1B4_5729 at 167.999 kb on + strand at 168.094 kb on - strand, within Pf1N1B4_5729 at 168.194 kb on + strand, within Pf1N1B4_5729 at 168.194 kb on + strand, within Pf1N1B4_5729 at 168.320 kb on + strand, within Pf1N1B4_5729 at 168.320 kb on + strand, within Pf1N1B4_5729 at 168.572 kb on + strand, within Pf1N1B4_5729 at 168.573 kb on - strand, within Pf1N1B4_5729 at 168.635 kb on + strand, within Pf1N1B4_5729 at 168.635 kb on + strand, within Pf1N1B4_5729 at 168.806 kb on + strand, within Pf1N1B4_5729 at 168.806 kb on + strand, within Pf1N1B4_5729 at 168.806 kb on + strand at 168.866 kb on + strand, within Pf1N1B4_5729 at 168.866 kb on + strand, within Pf1N1B4_5729 at 169.127 kb on + strand, within Pf1N1B4_5729 at 169.127 kb on + strand, within Pf1N1B4_5729 at 169.127 kb on + strand, within Pf1N1B4_5729 at 169.128 kb on - strand at 169.146 kb on - strand, within Pf1N1B4_5729 at 169.244 kb on + strand, within Pf1N1B4_5729 at 169.325 kb on + strand, within Pf1N1B4_5729 at 169.466 kb on + strand at 169.467 kb on - strand at 169.561 kb on - strand at 169.606 kb on + strand at 169.607 kb on - strand at 169.607 kb on - strand at 169.607 kb on - strand at 169.607 kb on - strand at 169.607 kb on - strand at 169.607 kb on - strand at 169.610 kb on + strand at 169.610 kb on + strand at 169.611 kb on - strand at 169.611 kb on - strand at 169.611 kb on - strand at 169.611 kb on - strand at 169.611 kb on - strand at 169.612 kb on + strand at 169.613 kb on - strand at 169.613 kb on - strand at 169.613 kb on - strand at 169.613 kb on - strand at 169.613 kb on - strand at 169.759 kb on - strand at 169.783 kb on + strand at 169.784 kb on - strand at 169.784 kb on - strand at 169.784 kb on - strand at 169.844 kb on - strand at 170.083 kb on + strand, within Pf1N1B4_5730 at 170.160 kb on + strand, within Pf1N1B4_5730 at 170.161 kb on - strand, within Pf1N1B4_5730 at 170.161 kb on - strand, within Pf1N1B4_5730 at 170.329 kb on - strand, within Pf1N1B4_5730
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 166,906 + Pf1N1B4_5727 0.32 -0.0 167,781 - Pf1N1B4_5729 0.10 -0.0 167,781 - Pf1N1B4_5729 0.10 -0.0 167,781 - -0.0 167,781 - Pf1N1B4_5729 0.10 -5.2 167,891 + Pf1N1B4_5729 0.16 -0.0 167,891 + Pf1N1B4_5729 0.16 -0.0 167,891 + Pf1N1B4_5729 0.16 -0.0 167,894 + Pf1N1B4_5729 0.16 -0.0 167,999 + -0.0 168,094 - Pf1N1B4_5729 0.26 -0.0 168,194 + Pf1N1B4_5729 0.31 -6.8 168,194 + Pf1N1B4_5729 0.31 -3.3 168,320 + Pf1N1B4_5729 0.37 -0.0 168,320 + Pf1N1B4_5729 0.37 -3.7 168,572 + Pf1N1B4_5729 0.49 -0.0 168,573 - Pf1N1B4_5729 0.50 -4.8 168,635 + Pf1N1B4_5729 0.53 -3.3 168,635 + Pf1N1B4_5729 0.53 -0.0 168,806 + Pf1N1B4_5729 0.61 -2.3 168,806 + Pf1N1B4_5729 0.61 -0.0 168,806 + -0.0 168,866 + Pf1N1B4_5729 0.64 -0.0 168,866 + Pf1N1B4_5729 0.64 -2.4 169,127 + Pf1N1B4_5729 0.77 -0.0 169,127 + Pf1N1B4_5729 0.77 -0.0 169,127 + Pf1N1B4_5729 0.77 -0.0 169,128 - -0.0 169,146 - Pf1N1B4_5729 0.78 -0.0 169,244 + Pf1N1B4_5729 0.83 -3.4 169,325 + Pf1N1B4_5729 0.87 -5.0 169,466 + -0.0 169,467 - -3.1 169,561 - -0.0 169,606 + -0.0 169,607 - -3.4 169,607 - -4.0 169,607 - -1.4 169,607 - -0.0 169,607 - -0.0 169,607 - -0.0 169,610 + -0.0 169,610 + -0.0 169,611 - -0.0 169,611 - -4.8 169,611 - -2.6 169,611 - -0.0 169,611 - -0.0 169,612 + -0.0 169,613 - -0.0 169,613 - -0.0 169,613 - -1.5 169,613 - -0.0 169,613 - -0.0 169,759 - -0.0 169,783 + -0.0 169,784 - -3.3 169,784 - -0.0 169,784 - -3.0 169,844 - +3.0 170,083 + Pf1N1B4_5730 0.22 -0.0 170,160 + Pf1N1B4_5730 0.29 -0.0 170,161 - Pf1N1B4_5730 0.30 +0.3 170,161 - Pf1N1B4_5730 0.30 -0.7 170,329 - Pf1N1B4_5730 0.46 -0.0
Or see this region's nucleotide sequence