Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5627 and Pf1N1B4_5628 overlap by 1 nucleotides Pf1N1B4_5628 and Pf1N1B4_5629 are separated by 14 nucleotides
Pf1N1B4_5627 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 54,260 to 55,321
_5627
Pf1N1B4_5628 - Oligopeptide transport system permease protein OppC (TC 3.A.1.5.1), at 55,321 to 56,343
_5628
Pf1N1B4_5629 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 56,358 to 57,932
_5629
Position (kb) 55
56
57 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 54.662 kb on + strand, within Pf1N1B4_5627 at 54.663 kb on - strand at 54.891 kb on - strand, within Pf1N1B4_5627 at 55.400 kb on + strand at 55.486 kb on + strand, within Pf1N1B4_5628 at 55.487 kb on - strand, within Pf1N1B4_5628 at 55.564 kb on + strand, within Pf1N1B4_5628 at 55.584 kb on + strand, within Pf1N1B4_5628 at 55.643 kb on - strand, within Pf1N1B4_5628 at 55.892 kb on - strand, within Pf1N1B4_5628 at 56.023 kb on + strand, within Pf1N1B4_5628 at 56.024 kb on - strand, within Pf1N1B4_5628 at 56.024 kb on - strand, within Pf1N1B4_5628 at 56.024 kb on - strand, within Pf1N1B4_5628 at 56.765 kb on - strand, within Pf1N1B4_5629 at 57.096 kb on + strand, within Pf1N1B4_5629 at 57.096 kb on + strand, within Pf1N1B4_5629 at 57.097 kb on - strand, within Pf1N1B4_5629 at 57.098 kb on + strand, within Pf1N1B4_5629 at 57.099 kb on - strand, within Pf1N1B4_5629 at 57.099 kb on - strand, within Pf1N1B4_5629 at 57.099 kb on - strand, within Pf1N1B4_5629 at 57.106 kb on + strand, within Pf1N1B4_5629 at 57.107 kb on - strand, within Pf1N1B4_5629 at 57.110 kb on - strand, within Pf1N1B4_5629 at 57.229 kb on - strand, within Pf1N1B4_5629
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 54,662 + Pf1N1B4_5627 0.38 -0.0 54,663 - -0.5 54,891 - Pf1N1B4_5627 0.59 -0.0 55,400 + -0.5 55,486 + Pf1N1B4_5628 0.16 -3.2 55,487 - Pf1N1B4_5628 0.16 -0.0 55,564 + Pf1N1B4_5628 0.24 -0.0 55,584 + Pf1N1B4_5628 0.26 -0.0 55,643 - Pf1N1B4_5628 0.31 -2.8 55,892 - Pf1N1B4_5628 0.56 -0.0 56,023 + Pf1N1B4_5628 0.69 -4.4 56,024 - Pf1N1B4_5628 0.69 -3.3 56,024 - Pf1N1B4_5628 0.69 -2.3 56,024 - Pf1N1B4_5628 0.69 -2.3 56,765 - Pf1N1B4_5629 0.26 -3.3 57,096 + Pf1N1B4_5629 0.47 -0.0 57,096 + Pf1N1B4_5629 0.47 -0.0 57,097 - Pf1N1B4_5629 0.47 -0.0 57,098 + Pf1N1B4_5629 0.47 -0.5 57,099 - Pf1N1B4_5629 0.47 -1.4 57,099 - Pf1N1B4_5629 0.47 -0.0 57,099 - Pf1N1B4_5629 0.47 -2.4 57,106 + Pf1N1B4_5629 0.47 -3.4 57,107 - Pf1N1B4_5629 0.48 -0.0 57,110 - Pf1N1B4_5629 0.48 -2.2 57,229 - Pf1N1B4_5629 0.55 -2.1
Or see this region's nucleotide sequence