Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5610 and Pf1N1B4_5611 are separated by 19 nucleotides Pf1N1B4_5611 and Pf1N1B4_5612 are separated by 37 nucleotides Pf1N1B4_5612 and Pf1N1B4_5613 are separated by 0 nucleotides
Pf1N1B4_5610 - Probable acyl-CoA dehydrogenase (EC 1.3.99.3), at 35,870 to 37,033
_5610
Pf1N1B4_5611 - L-carnitine dehydratase/bile acid-inducible protein F (EC 2.8.3.16), at 37,053 to 38,255
_5611
Pf1N1B4_5612 - Electron transfer flavoprotein, beta subunit, at 38,293 to 39,042
_5612
Pf1N1B4_5613 - Electron transfer flavoprotein, alpha subunit, at 39,043 to 39,999
_5613
Position (kb) 37
38
39 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 36.112 kb on + strand, within Pf1N1B4_5610 at 36.141 kb on - strand, within Pf1N1B4_5610 at 36.189 kb on - strand at 36.192 kb on - strand, within Pf1N1B4_5610 at 36.192 kb on - strand, within Pf1N1B4_5610 at 36.308 kb on + strand, within Pf1N1B4_5610 at 36.308 kb on + strand, within Pf1N1B4_5610 at 36.313 kb on - strand, within Pf1N1B4_5610 at 36.330 kb on + strand, within Pf1N1B4_5610 at 36.330 kb on + strand at 36.579 kb on - strand, within Pf1N1B4_5610 at 36.839 kb on + strand, within Pf1N1B4_5610 at 36.840 kb on - strand, within Pf1N1B4_5610 at 36.992 kb on + strand at 36.993 kb on - strand at 36.993 kb on - strand at 36.993 kb on - strand at 37.031 kb on + strand at 37.032 kb on - strand at 37.032 kb on - strand at 37.590 kb on + strand at 37.591 kb on - strand, within Pf1N1B4_5611 at 37.705 kb on - strand, within Pf1N1B4_5611 at 37.708 kb on - strand, within Pf1N1B4_5611 at 38.270 kb on + strand at 38.306 kb on + strand at 38.432 kb on + strand, within Pf1N1B4_5612 at 38.432 kb on + strand, within Pf1N1B4_5612
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 36,112 + Pf1N1B4_5610 0.21 -0.0 36,141 - Pf1N1B4_5610 0.23 -1.3 36,189 - -0.0 36,192 - Pf1N1B4_5610 0.28 -0.0 36,192 - Pf1N1B4_5610 0.28 -5.6 36,308 + Pf1N1B4_5610 0.38 -0.0 36,308 + Pf1N1B4_5610 0.38 -0.0 36,313 - Pf1N1B4_5610 0.38 -2.8 36,330 + Pf1N1B4_5610 0.40 -0.0 36,330 + -0.0 36,579 - Pf1N1B4_5610 0.61 -1.7 36,839 + Pf1N1B4_5610 0.83 -2.0 36,840 - Pf1N1B4_5610 0.83 -0.0 36,992 + -0.0 36,993 - -1.3 36,993 - -2.2 36,993 - -0.0 37,031 + -3.5 37,032 - -0.0 37,032 - -5.1 37,590 + -0.0 37,591 - Pf1N1B4_5611 0.45 -0.0 37,705 - Pf1N1B4_5611 0.54 -3.4 37,708 - Pf1N1B4_5611 0.54 -0.0 38,270 + -1.4 38,306 + -1.6 38,432 + Pf1N1B4_5612 0.19 -0.9 38,432 + Pf1N1B4_5612 0.19 -2.2
Or see this region's nucleotide sequence