Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_51 and Pf1N1B4_52 are separated by 337 nucleotides Pf1N1B4_52 and Pf1N1B4_53 are separated by 6 nucleotides Pf1N1B4_53 and Pf1N1B4_54 are separated by 194 nucleotides
Pf1N1B4_51 - hypothetical protein, at 57,079 to 57,348
_51
Pf1N1B4_52 - FIG00956056: hypothetical protein, at 57,686 to 58,144
_52
Pf1N1B4_53 - hypothetical protein, at 58,151 to 58,342
_53
Pf1N1B4_54 - Cyclohexadienyl dehydratase (EC 4.2.1.51)(EC 4.2.1.91), at 58,537 to 59,328
_54
Position (kb) 58
59 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand, within Pf1N1B4_51 at 57.222 kb on - strand, within Pf1N1B4_51 at 57.222 kb on - strand, within Pf1N1B4_51 at 57.409 kb on + strand at 57.432 kb on - strand at 57.504 kb on - strand at 57.512 kb on + strand at 57.512 kb on + strand at 57.513 kb on - strand at 57.603 kb on - strand at 57.603 kb on - strand at 57.717 kb on - strand at 57.717 kb on - strand at 57.875 kb on - strand, within Pf1N1B4_52 at 58.055 kb on - strand, within Pf1N1B4_52 at 58.072 kb on + strand, within Pf1N1B4_52 at 58.093 kb on + strand, within Pf1N1B4_52 at 58.142 kb on + strand at 58.182 kb on + strand, within Pf1N1B4_53 at 58.186 kb on + strand, within Pf1N1B4_53 at 58.202 kb on + strand, within Pf1N1B4_53 at 58.202 kb on + strand, within Pf1N1B4_53 at 58.206 kb on + strand at 58.206 kb on + strand, within Pf1N1B4_53 at 58.206 kb on + strand, within Pf1N1B4_53 at 58.206 kb on + strand, within Pf1N1B4_53 at 58.206 kb on + strand, within Pf1N1B4_53 at 58.207 kb on - strand, within Pf1N1B4_53 at 58.272 kb on + strand, within Pf1N1B4_53 at 58.352 kb on + strand at 58.388 kb on + strand at 58.389 kb on - strand at 58.389 kb on - strand at 58.426 kb on + strand at 58.427 kb on - strand at 58.427 kb on - strand at 58.548 kb on - strand at 58.684 kb on - strand, within Pf1N1B4_54 at 58.684 kb on - strand, within Pf1N1B4_54 at 58.684 kb on - strand, within Pf1N1B4_54 at 58.709 kb on - strand, within Pf1N1B4_54 at 59.132 kb on - strand at 59.145 kb on + strand, within Pf1N1B4_54 at 59.181 kb on + strand, within Pf1N1B4_54 at 59.182 kb on - strand, within Pf1N1B4_54 at 59.189 kb on - strand, within Pf1N1B4_54 at 59.203 kb on + strand, within Pf1N1B4_54 at 59.203 kb on + strand, within Pf1N1B4_54 at 59.239 kb on + strand, within Pf1N1B4_54 at 59.239 kb on + strand, within Pf1N1B4_54 at 59.239 kb on + strand, within Pf1N1B4_54 at 59.239 kb on + strand, within Pf1N1B4_54 at 59.239 kb on + strand at 59.239 kb on + strand, within Pf1N1B4_54 at 59.239 kb on + strand, within Pf1N1B4_54 at 59.239 kb on + strand, within Pf1N1B4_54 at 59.282 kb on - strand at 59.282 kb on - strand at 59.322 kb on + strand at 59.322 kb on + strand at 59.326 kb on + strand at 59.326 kb on + strand at 59.326 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 57,221 + Pf1N1B4_51 0.53 -0.0 57,221 + Pf1N1B4_51 0.53 -0.0 57,221 + -1.4 57,221 + Pf1N1B4_51 0.53 -0.0 57,221 + Pf1N1B4_51 0.53 -0.0 57,221 + Pf1N1B4_51 0.53 -2.9 57,221 + Pf1N1B4_51 0.53 -0.0 57,222 - Pf1N1B4_51 0.53 -0.0 57,222 - Pf1N1B4_51 0.53 -0.0 57,409 + -0.0 57,432 - -0.0 57,504 - -0.0 57,512 + -1.8 57,512 + -1.8 57,513 - -0.0 57,603 - -0.5 57,603 - -0.0 57,717 - -2.8 57,717 - -1.8 57,875 - Pf1N1B4_52 0.41 -0.0 58,055 - Pf1N1B4_52 0.80 -0.0 58,072 + Pf1N1B4_52 0.84 -0.0 58,093 + Pf1N1B4_52 0.89 -0.0 58,142 + -1.6 58,182 + Pf1N1B4_53 0.16 -0.0 58,186 + Pf1N1B4_53 0.18 -0.0 58,202 + Pf1N1B4_53 0.27 -2.7 58,202 + Pf1N1B4_53 0.27 -0.0 58,206 + -0.0 58,206 + Pf1N1B4_53 0.29 -2.3 58,206 + Pf1N1B4_53 0.29 -0.0 58,206 + Pf1N1B4_53 0.29 -3.3 58,206 + Pf1N1B4_53 0.29 -0.0 58,207 - Pf1N1B4_53 0.29 -0.0 58,272 + Pf1N1B4_53 0.63 -0.0 58,352 + -1.0 58,388 + -0.0 58,389 - -0.0 58,389 - -0.0 58,426 + -2.4 58,427 - -2.2 58,427 - -0.0 58,548 - -1.7 58,684 - Pf1N1B4_54 0.19 -2.0 58,684 - Pf1N1B4_54 0.19 -0.0 58,684 - Pf1N1B4_54 0.19 -3.2 58,709 - Pf1N1B4_54 0.22 -0.0 59,132 - -5.8 59,145 + Pf1N1B4_54 0.77 -2.7 59,181 + Pf1N1B4_54 0.81 -0.0 59,182 - Pf1N1B4_54 0.81 -0.0 59,189 - Pf1N1B4_54 0.82 -3.0 59,203 + Pf1N1B4_54 0.84 -0.0 59,203 + Pf1N1B4_54 0.84 -0.0 59,239 + Pf1N1B4_54 0.89 -0.0 59,239 + Pf1N1B4_54 0.89 -0.0 59,239 + Pf1N1B4_54 0.89 -0.5 59,239 + Pf1N1B4_54 0.89 -3.6 59,239 + -0.0 59,239 + Pf1N1B4_54 0.89 -0.0 59,239 + Pf1N1B4_54 0.89 -2.4 59,239 + Pf1N1B4_54 0.89 -0.0 59,282 - -0.0 59,282 - -0.0 59,322 + -0.0 59,322 + -0.0 59,326 + -0.5 59,326 + -0.0 59,326 + -0.0
Or see this region's nucleotide sequence