Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5139

Experiment: Nitrate reduction

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5138 and Pf1N1B4_5139 are separated by 113 nucleotidesPf1N1B4_5139 and Pf1N1B4_5140 are separated by 348 nucleotides Pf1N1B4_5138 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 5,717,408 to 5,718,499 _5138 Pf1N1B4_5139 - Gamma-aminobutyrate permease, at 5,718,613 to 5,720,118 _5139 Pf1N1B4_5140 - Gifsy-2 prophage protein, at 5,720,467 to 5,721,156 _5140 Position (kb) 5718 5719 5720 5721Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 5717.633 kb on + strand, within Pf1N1B4_5138at 5717.694 kb on + strand, within Pf1N1B4_5138at 5717.721 kb on + strand, within Pf1N1B4_5138at 5717.721 kb on + strand, within Pf1N1B4_5138at 5717.767 kb on - strandat 5717.911 kb on - strand, within Pf1N1B4_5138at 5718.492 kb on + strandat 5718.492 kb on + strandat 5718.492 kb on + strandat 5718.598 kb on - strandat 5718.607 kb on + strandat 5718.607 kb on + strandat 5718.608 kb on - strandat 5718.790 kb on - strand, within Pf1N1B4_5139at 5718.790 kb on - strand, within Pf1N1B4_5139at 5718.950 kb on + strand, within Pf1N1B4_5139at 5719.089 kb on + strand, within Pf1N1B4_5139at 5719.091 kb on + strand, within Pf1N1B4_5139at 5719.091 kb on + strandat 5719.092 kb on - strand, within Pf1N1B4_5139at 5719.141 kb on + strand, within Pf1N1B4_5139at 5719.148 kb on - strand, within Pf1N1B4_5139at 5719.568 kb on + strand, within Pf1N1B4_5139at 5719.591 kb on - strand, within Pf1N1B4_5139at 5719.663 kb on + strand, within Pf1N1B4_5139at 5719.663 kb on + strandat 5719.663 kb on + strand, within Pf1N1B4_5139at 5719.664 kb on - strand, within Pf1N1B4_5139at 5719.664 kb on - strand, within Pf1N1B4_5139at 5719.664 kb on - strand, within Pf1N1B4_5139at 5719.664 kb on - strand, within Pf1N1B4_5139at 5719.664 kb on - strand, within Pf1N1B4_5139at 5719.770 kb on - strandat 5720.228 kb on + strandat 5720.229 kb on - strandat 5720.289 kb on + strandat 5720.545 kb on + strand, within Pf1N1B4_5140at 5720.546 kb on - strandat 5720.564 kb on + strand, within Pf1N1B4_5140at 5720.572 kb on + strand, within Pf1N1B4_5140at 5720.572 kb on + strandat 5720.572 kb on + strand, within Pf1N1B4_5140at 5720.572 kb on + strand, within Pf1N1B4_5140at 5720.573 kb on - strand, within Pf1N1B4_5140at 5720.573 kb on - strandat 5720.719 kb on - strand, within Pf1N1B4_5140at 5720.815 kb on + strand, within Pf1N1B4_5140at 5720.815 kb on + strand, within Pf1N1B4_5140at 5720.816 kb on - strandat 5720.816 kb on - strandat 5720.816 kb on - strand, within Pf1N1B4_5140at 5720.945 kb on + strand, within Pf1N1B4_5140at 5720.945 kb on + strand, within Pf1N1B4_5140at 5720.946 kb on - strand, within Pf1N1B4_5140at 5720.946 kb on - strand, within Pf1N1B4_5140at 5720.950 kb on - strand, within Pf1N1B4_5140at 5720.951 kb on + strand, within Pf1N1B4_5140at 5720.952 kb on - strandat 5720.952 kb on - strand, within Pf1N1B4_5140at 5720.952 kb on - strandat 5720.952 kb on - strand, within Pf1N1B4_5140at 5721.012 kb on + strand, within Pf1N1B4_5140at 5721.038 kb on + strand, within Pf1N1B4_5140at 5721.038 kb on + strand, within Pf1N1B4_5140at 5721.039 kb on - strand, within Pf1N1B4_5140at 5721.039 kb on - strand, within Pf1N1B4_5140at 5721.039 kb on - strand, within Pf1N1B4_5140at 5721.039 kb on - strand, within Pf1N1B4_5140at 5721.045 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction
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5,717,633 + Pf1N1B4_5138 0.21 -0.1
5,717,694 + Pf1N1B4_5138 0.26 -2.0
5,717,721 + Pf1N1B4_5138 0.29 -1.8
5,717,721 + Pf1N1B4_5138 0.29 -0.1
5,717,767 - -0.1
5,717,911 - Pf1N1B4_5138 0.46 -0.1
5,718,492 + -0.1
5,718,492 + -0.5
5,718,492 + -0.1
5,718,598 - -2.1
5,718,607 + -0.1
5,718,607 + -1.7
5,718,608 - -0.1
5,718,790 - Pf1N1B4_5139 0.12 -4.7
5,718,790 - Pf1N1B4_5139 0.12 -0.1
5,718,950 + Pf1N1B4_5139 0.22 -0.1
5,719,089 + Pf1N1B4_5139 0.32 -2.5
5,719,091 + Pf1N1B4_5139 0.32 -0.1
5,719,091 + -0.1
5,719,092 - Pf1N1B4_5139 0.32 -3.3
5,719,141 + Pf1N1B4_5139 0.35 -2.6
5,719,148 - Pf1N1B4_5139 0.36 -0.1
5,719,568 + Pf1N1B4_5139 0.63 -0.1
5,719,591 - Pf1N1B4_5139 0.65 -1.4
5,719,663 + Pf1N1B4_5139 0.70 -0.1
5,719,663 + -0.1
5,719,663 + Pf1N1B4_5139 0.70 -0.1
5,719,664 - Pf1N1B4_5139 0.70 -0.1
5,719,664 - Pf1N1B4_5139 0.70 -0.1
5,719,664 - Pf1N1B4_5139 0.70 -2.1
5,719,664 - Pf1N1B4_5139 0.70 -2.4
5,719,664 - Pf1N1B4_5139 0.70 -0.1
5,719,770 - -0.1
5,720,228 + -0.1
5,720,229 - -0.1
5,720,289 + -0.1
5,720,545 + Pf1N1B4_5140 0.11 -0.1
5,720,546 - -2.1
5,720,564 + Pf1N1B4_5140 0.14 -0.1
5,720,572 + Pf1N1B4_5140 0.15 -5.0
5,720,572 + -0.1
5,720,572 + Pf1N1B4_5140 0.15 -0.1
5,720,572 + Pf1N1B4_5140 0.15 -1.1
5,720,573 - Pf1N1B4_5140 0.15 -4.1
5,720,573 - -1.1
5,720,719 - Pf1N1B4_5140 0.37 -3.2
5,720,815 + Pf1N1B4_5140 0.50 -0.1
5,720,815 + Pf1N1B4_5140 0.50 -0.1
5,720,816 - -1.6
5,720,816 - -0.1
5,720,816 - Pf1N1B4_5140 0.51 -3.4
5,720,945 + Pf1N1B4_5140 0.69 -0.1
5,720,945 + Pf1N1B4_5140 0.69 -0.1
5,720,946 - Pf1N1B4_5140 0.69 -0.1
5,720,946 - Pf1N1B4_5140 0.69 -0.1
5,720,950 - Pf1N1B4_5140 0.70 -0.1
5,720,951 + Pf1N1B4_5140 0.70 -0.1
5,720,952 - -0.1
5,720,952 - Pf1N1B4_5140 0.70 -0.1
5,720,952 - -0.1
5,720,952 - Pf1N1B4_5140 0.70 -0.1
5,721,012 + Pf1N1B4_5140 0.79 -1.7
5,721,038 + Pf1N1B4_5140 0.83 -0.1
5,721,038 + Pf1N1B4_5140 0.83 -0.1
5,721,039 - Pf1N1B4_5140 0.83 -1.6
5,721,039 - Pf1N1B4_5140 0.83 -0.1
5,721,039 - Pf1N1B4_5140 0.83 -1.8
5,721,039 - Pf1N1B4_5140 0.83 -0.1
5,721,045 - -3.2

Or see this region's nucleotide sequence