Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5136

Experiment: Nitrate reduction

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5134 and Pf1N1B4_5135 overlap by 8 nucleotidesPf1N1B4_5135 and Pf1N1B4_5136 are separated by 124 nucleotidesPf1N1B4_5136 and Pf1N1B4_5137 are separated by 27 nucleotides Pf1N1B4_5134 - 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157), at 5,713,194 to 5,714,045 _5134 Pf1N1B4_5135 - Transcriptional regulator, TetR family, at 5,714,038 to 5,714,685 _5135 Pf1N1B4_5136 - Carboxynorspermidine dehydrogenase, at 5,714,810 to 5,716,045 _5136 Pf1N1B4_5137 - Carboxynorspermidine decarboxylase, putative (EC 4.1.1.-), at 5,716,073 to 5,717,170 _5137 Position (kb) 5714 5715 5716 5717Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 5713.936 kb on - strand, within Pf1N1B4_5134at 5714.038 kb on - strandat 5714.168 kb on - strand, within Pf1N1B4_5135at 5714.222 kb on - strand, within Pf1N1B4_5135at 5714.222 kb on - strand, within Pf1N1B4_5135at 5714.436 kb on - strand, within Pf1N1B4_5135at 5714.438 kb on + strand, within Pf1N1B4_5135at 5714.439 kb on - strand, within Pf1N1B4_5135at 5714.439 kb on - strand, within Pf1N1B4_5135at 5714.533 kb on + strand, within Pf1N1B4_5135at 5714.616 kb on + strand, within Pf1N1B4_5135at 5714.684 kb on - strandat 5714.771 kb on - strandat 5714.985 kb on + strand, within Pf1N1B4_5136at 5714.986 kb on - strand, within Pf1N1B4_5136at 5714.986 kb on - strand, within Pf1N1B4_5136at 5715.042 kb on + strand, within Pf1N1B4_5136at 5715.043 kb on - strand, within Pf1N1B4_5136at 5715.043 kb on - strand, within Pf1N1B4_5136at 5715.043 kb on - strand, within Pf1N1B4_5136at 5715.043 kb on - strand, within Pf1N1B4_5136at 5715.043 kb on - strand, within Pf1N1B4_5136at 5715.234 kb on - strand, within Pf1N1B4_5136at 5715.252 kb on + strand, within Pf1N1B4_5136at 5715.252 kb on + strand, within Pf1N1B4_5136at 5715.252 kb on + strand, within Pf1N1B4_5136at 5715.253 kb on - strand, within Pf1N1B4_5136at 5715.253 kb on - strand, within Pf1N1B4_5136at 5715.253 kb on - strandat 5715.253 kb on - strand, within Pf1N1B4_5136at 5715.253 kb on - strand, within Pf1N1B4_5136at 5715.253 kb on - strand, within Pf1N1B4_5136at 5715.340 kb on - strand, within Pf1N1B4_5136at 5715.340 kb on - strand, within Pf1N1B4_5136at 5715.487 kb on - strand, within Pf1N1B4_5136at 5715.536 kb on - strand, within Pf1N1B4_5136at 5715.546 kb on + strand, within Pf1N1B4_5136at 5715.657 kb on + strandat 5715.866 kb on + strand, within Pf1N1B4_5136at 5715.867 kb on - strand, within Pf1N1B4_5136at 5715.867 kb on - strand, within Pf1N1B4_5136at 5715.867 kb on - strand, within Pf1N1B4_5136at 5715.956 kb on - strandat 5716.028 kb on - strandat 5716.074 kb on + strandat 5716.093 kb on - strandat 5716.093 kb on - strandat 5716.308 kb on + strand, within Pf1N1B4_5137at 5716.308 kb on + strandat 5716.308 kb on + strand, within Pf1N1B4_5137at 5716.308 kb on + strandat 5716.308 kb on + strand, within Pf1N1B4_5137at 5716.308 kb on + strand, within Pf1N1B4_5137at 5716.308 kb on + strand, within Pf1N1B4_5137at 5716.309 kb on - strand, within Pf1N1B4_5137at 5716.419 kb on + strand, within Pf1N1B4_5137at 5716.851 kb on + strandat 5716.942 kb on - strand, within Pf1N1B4_5137at 5717.005 kb on + strand, within Pf1N1B4_5137at 5717.006 kb on - strand, within Pf1N1B4_5137at 5717.006 kb on - strand, within Pf1N1B4_5137at 5717.006 kb on - strand, within Pf1N1B4_5137at 5717.019 kb on + strand, within Pf1N1B4_5137at 5717.019 kb on + strand, within Pf1N1B4_5137at 5717.019 kb on + strand, within Pf1N1B4_5137at 5717.019 kb on + strand, within Pf1N1B4_5137at 5717.019 kb on + strand, within Pf1N1B4_5137at 5717.019 kb on + strand, within Pf1N1B4_5137at 5717.019 kb on + strand, within Pf1N1B4_5137at 5717.020 kb on - strandat 5717.020 kb on - strandat 5717.020 kb on - strand, within Pf1N1B4_5137at 5717.020 kb on - strand, within Pf1N1B4_5137at 5717.020 kb on - strand, within Pf1N1B4_5137

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction
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5,713,936 - Pf1N1B4_5134 0.87 -0.1
5,714,038 - -0.1
5,714,168 - Pf1N1B4_5135 0.20 -0.1
5,714,222 - Pf1N1B4_5135 0.28 -0.1
5,714,222 - Pf1N1B4_5135 0.28 -0.1
5,714,436 - Pf1N1B4_5135 0.61 -2.8
5,714,438 + Pf1N1B4_5135 0.62 -0.1
5,714,439 - Pf1N1B4_5135 0.62 -2.2
5,714,439 - Pf1N1B4_5135 0.62 -2.2
5,714,533 + Pf1N1B4_5135 0.76 -0.1
5,714,616 + Pf1N1B4_5135 0.89 -0.1
5,714,684 - -1.9
5,714,771 - -0.1
5,714,985 + Pf1N1B4_5136 0.14 -0.1
5,714,986 - Pf1N1B4_5136 0.14 -0.1
5,714,986 - Pf1N1B4_5136 0.14 -0.1
5,715,042 + Pf1N1B4_5136 0.19 -0.1
5,715,043 - Pf1N1B4_5136 0.19 -0.1
5,715,043 - Pf1N1B4_5136 0.19 -2.5
5,715,043 - Pf1N1B4_5136 0.19 -2.4
5,715,043 - Pf1N1B4_5136 0.19 -3.8
5,715,043 - Pf1N1B4_5136 0.19 -0.1
5,715,234 - Pf1N1B4_5136 0.34 -0.1
5,715,252 + Pf1N1B4_5136 0.36 -4.4
5,715,252 + Pf1N1B4_5136 0.36 -0.1
5,715,252 + Pf1N1B4_5136 0.36 -0.1
5,715,253 - Pf1N1B4_5136 0.36 -2.3
5,715,253 - Pf1N1B4_5136 0.36 -0.1
5,715,253 - -3.1
5,715,253 - Pf1N1B4_5136 0.36 -3.5
5,715,253 - Pf1N1B4_5136 0.36 -0.1
5,715,253 - Pf1N1B4_5136 0.36 -0.1
5,715,340 - Pf1N1B4_5136 0.43 -0.1
5,715,340 - Pf1N1B4_5136 0.43 -0.1
5,715,487 - Pf1N1B4_5136 0.55 -0.1
5,715,536 - Pf1N1B4_5136 0.59 +1.2
5,715,546 + Pf1N1B4_5136 0.60 -0.1
5,715,657 + -0.1
5,715,866 + Pf1N1B4_5136 0.85 -4.1
5,715,867 - Pf1N1B4_5136 0.86 -0.1
5,715,867 - Pf1N1B4_5136 0.86 -2.4
5,715,867 - Pf1N1B4_5136 0.86 -4.7
5,715,956 - -0.1
5,716,028 - -3.5
5,716,074 + -2.6
5,716,093 - -0.1
5,716,093 - -3.4
5,716,308 + Pf1N1B4_5137 0.21 -2.7
5,716,308 + -1.0
5,716,308 + Pf1N1B4_5137 0.21 -2.4
5,716,308 + -0.1
5,716,308 + Pf1N1B4_5137 0.21 -0.1
5,716,308 + Pf1N1B4_5137 0.21 -0.1
5,716,308 + Pf1N1B4_5137 0.21 -0.1
5,716,309 - Pf1N1B4_5137 0.21 -0.1
5,716,419 + Pf1N1B4_5137 0.32 -2.5
5,716,851 + -0.1
5,716,942 - Pf1N1B4_5137 0.79 -0.1
5,717,005 + Pf1N1B4_5137 0.85 -4.0
5,717,006 - Pf1N1B4_5137 0.85 -0.1
5,717,006 - Pf1N1B4_5137 0.85 -0.1
5,717,006 - Pf1N1B4_5137 0.85 -2.3
5,717,019 + Pf1N1B4_5137 0.86 -0.1
5,717,019 + Pf1N1B4_5137 0.86 -0.1
5,717,019 + Pf1N1B4_5137 0.86 -0.1
5,717,019 + Pf1N1B4_5137 0.86 -3.7
5,717,019 + Pf1N1B4_5137 0.86 -0.1
5,717,019 + Pf1N1B4_5137 0.86 -5.3
5,717,019 + Pf1N1B4_5137 0.86 -4.7
5,717,020 - -3.9
5,717,020 - -6.1
5,717,020 - Pf1N1B4_5137 0.86 -0.1
5,717,020 - Pf1N1B4_5137 0.86 -4.1
5,717,020 - Pf1N1B4_5137 0.86 -0.1

Or see this region's nucleotide sequence