Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_48 and Pf1N1B4_49 are separated by 30 nucleotides Pf1N1B4_49 and Pf1N1B4_50 are separated by 163 nucleotides Pf1N1B4_50 and Pf1N1B4_51 are separated by 89 nucleotides Pf1N1B4_51 and Pf1N1B4_52 are separated by 337 nucleotides Pf1N1B4_52 and Pf1N1B4_53 are separated by 6 nucleotides
Pf1N1B4_48 - hypothetical protein, at 55,996 to 56,406
_48
Pf1N1B4_49 - hypothetical protein, at 56,437 to 56,643
_49
Pf1N1B4_50 - Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4), at 56,807 to 56,989
_50
Pf1N1B4_51 - hypothetical protein, at 57,079 to 57,348
_51
Pf1N1B4_52 - FIG00956056: hypothetical protein, at 57,686 to 58,144
_52
Pf1N1B4_53 - hypothetical protein, at 58,151 to 58,342
_53
Position (kb) 57
58 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 56.516 kb on + strand, within Pf1N1B4_49 at 56.517 kb on - strand, within Pf1N1B4_49 at 56.520 kb on - strand, within Pf1N1B4_49 at 56.559 kb on + strand, within Pf1N1B4_49 at 56.559 kb on + strand, within Pf1N1B4_49 at 56.560 kb on - strand, within Pf1N1B4_49 at 56.560 kb on - strand at 56.560 kb on - strand, within Pf1N1B4_49 at 56.588 kb on + strand, within Pf1N1B4_49 at 56.588 kb on + strand, within Pf1N1B4_49 at 56.767 kb on + strand at 56.784 kb on + strand at 56.837 kb on + strand, within Pf1N1B4_50 at 56.837 kb on + strand, within Pf1N1B4_50 at 56.837 kb on + strand, within Pf1N1B4_50 at 56.837 kb on + strand, within Pf1N1B4_50 at 56.838 kb on - strand, within Pf1N1B4_50 at 56.838 kb on - strand, within Pf1N1B4_50 at 56.838 kb on - strand, within Pf1N1B4_50 at 56.838 kb on - strand at 56.895 kb on + strand, within Pf1N1B4_50 at 56.895 kb on + strand at 56.895 kb on + strand, within Pf1N1B4_50 at 56.895 kb on + strand, within Pf1N1B4_50 at 56.895 kb on + strand, within Pf1N1B4_50 at 56.895 kb on + strand, within Pf1N1B4_50 at 56.896 kb on - strand at 56.896 kb on - strand, within Pf1N1B4_50 at 56.896 kb on - strand, within Pf1N1B4_50 at 56.896 kb on - strand, within Pf1N1B4_50 at 56.896 kb on - strand, within Pf1N1B4_50 at 57.074 kb on + strand at 57.087 kb on + strand at 57.087 kb on + strand at 57.087 kb on + strand at 57.087 kb on + strand at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand, within Pf1N1B4_51 at 57.222 kb on - strand, within Pf1N1B4_51 at 57.222 kb on - strand, within Pf1N1B4_51 at 57.409 kb on + strand at 57.432 kb on - strand at 57.504 kb on - strand at 57.512 kb on + strand at 57.512 kb on + strand at 57.513 kb on - strand at 57.603 kb on - strand at 57.603 kb on - strand at 57.717 kb on - strand at 57.717 kb on - strand at 57.875 kb on - strand, within Pf1N1B4_52 at 58.055 kb on - strand, within Pf1N1B4_52 at 58.072 kb on + strand, within Pf1N1B4_52 at 58.093 kb on + strand, within Pf1N1B4_52 at 58.142 kb on + strand at 58.182 kb on + strand, within Pf1N1B4_53 at 58.186 kb on + strand, within Pf1N1B4_53 at 58.202 kb on + strand, within Pf1N1B4_53 at 58.202 kb on + strand, within Pf1N1B4_53 at 58.206 kb on + strand at 58.206 kb on + strand, within Pf1N1B4_53 at 58.206 kb on + strand, within Pf1N1B4_53 at 58.206 kb on + strand, within Pf1N1B4_53 at 58.206 kb on + strand, within Pf1N1B4_53 at 58.207 kb on - strand, within Pf1N1B4_53 at 58.272 kb on + strand, within Pf1N1B4_53
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 56,516 + Pf1N1B4_49 0.38 -0.0 56,517 - Pf1N1B4_49 0.39 -0.0 56,520 - Pf1N1B4_49 0.40 -0.0 56,559 + Pf1N1B4_49 0.59 -2.0 56,559 + Pf1N1B4_49 0.59 -0.0 56,560 - Pf1N1B4_49 0.59 -0.0 56,560 - -0.0 56,560 - Pf1N1B4_49 0.59 -0.0 56,588 + Pf1N1B4_49 0.73 -1.6 56,588 + Pf1N1B4_49 0.73 -2.3 56,767 + -0.0 56,784 + -0.0 56,837 + Pf1N1B4_50 0.16 -0.0 56,837 + Pf1N1B4_50 0.16 -0.0 56,837 + Pf1N1B4_50 0.16 -2.2 56,837 + Pf1N1B4_50 0.16 -0.0 56,838 - Pf1N1B4_50 0.17 -0.0 56,838 - Pf1N1B4_50 0.17 -0.0 56,838 - Pf1N1B4_50 0.17 -0.7 56,838 - -1.2 56,895 + Pf1N1B4_50 0.48 -0.0 56,895 + -0.0 56,895 + Pf1N1B4_50 0.48 -4.1 56,895 + Pf1N1B4_50 0.48 -0.0 56,895 + Pf1N1B4_50 0.48 -2.7 56,895 + Pf1N1B4_50 0.48 -0.0 56,896 - -2.0 56,896 - Pf1N1B4_50 0.49 -0.0 56,896 - Pf1N1B4_50 0.49 -0.0 56,896 - Pf1N1B4_50 0.49 -1.7 56,896 - Pf1N1B4_50 0.49 -0.0 57,074 + -0.0 57,087 + -0.0 57,087 + -0.0 57,087 + -0.0 57,087 + -0.0 57,221 + Pf1N1B4_51 0.53 -0.0 57,221 + Pf1N1B4_51 0.53 -0.0 57,221 + -1.4 57,221 + Pf1N1B4_51 0.53 -0.0 57,221 + Pf1N1B4_51 0.53 -0.0 57,221 + Pf1N1B4_51 0.53 -2.9 57,221 + Pf1N1B4_51 0.53 -0.0 57,222 - Pf1N1B4_51 0.53 -0.0 57,222 - Pf1N1B4_51 0.53 -0.0 57,409 + -0.0 57,432 - -0.0 57,504 - -0.0 57,512 + -1.8 57,512 + -1.8 57,513 - -0.0 57,603 - -0.5 57,603 - -0.0 57,717 - -2.8 57,717 - -1.8 57,875 - Pf1N1B4_52 0.41 -0.0 58,055 - Pf1N1B4_52 0.80 -0.0 58,072 + Pf1N1B4_52 0.84 -0.0 58,093 + Pf1N1B4_52 0.89 -0.0 58,142 + -1.6 58,182 + Pf1N1B4_53 0.16 -0.0 58,186 + Pf1N1B4_53 0.18 -0.0 58,202 + Pf1N1B4_53 0.27 -2.7 58,202 + Pf1N1B4_53 0.27 -0.0 58,206 + -0.0 58,206 + Pf1N1B4_53 0.29 -2.3 58,206 + Pf1N1B4_53 0.29 -0.0 58,206 + Pf1N1B4_53 0.29 -3.3 58,206 + Pf1N1B4_53 0.29 -0.0 58,207 - Pf1N1B4_53 0.29 -0.0 58,272 + Pf1N1B4_53 0.63 -0.0
Or see this region's nucleotide sequence