Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5028 and Pf1N1B4_5029 are separated by 216 nucleotides Pf1N1B4_5029 and Pf1N1B4_5030 are separated by 0 nucleotides Pf1N1B4_5030 and Pf1N1B4_5031 are separated by 3 nucleotides
Pf1N1B4_5028 - Enoyl-[acyl-carrier-protein] reductase [FMN] (EC 1.3.1.9), at 5,574,758 to 5,575,258
_5028
Pf1N1B4_5029 - Molybdenum ABC transporter, periplasmic molybdenum-binding protein ModA (TC 3.A.1.8.1), at 5,575,475 to 5,576,233
_5029
Pf1N1B4_5030 - Molybdenum transport system permease protein ModB (TC 3.A.1.8.1), at 5,576,234 to 5,576,914
_5030
Pf1N1B4_5031 - Molybdenum transport ATP-binding protein ModC (TC 3.A.1.8.1), at 5,576,918 to 5,577,997
_5031
Position (kb)
5576
5577 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 5575.256 kb on + strand at 5575.256 kb on + strand at 5575.412 kb on + strand at 5575.429 kb on - strand at 5575.431 kb on - strand at 5575.431 kb on - strand at 5575.778 kb on + strand, within Pf1N1B4_5029 at 5575.820 kb on + strand, within Pf1N1B4_5029 at 5575.820 kb on + strand, within Pf1N1B4_5029 at 5575.820 kb on + strand at 5575.821 kb on - strand, within Pf1N1B4_5029 at 5576.049 kb on - strand, within Pf1N1B4_5029 at 5576.138 kb on - strand, within Pf1N1B4_5029 at 5576.177 kb on + strand at 5576.231 kb on + strand at 5576.232 kb on - strand at 5576.426 kb on + strand, within Pf1N1B4_5030 at 5576.427 kb on - strand, within Pf1N1B4_5030 at 5576.427 kb on - strand, within Pf1N1B4_5030 at 5576.427 kb on - strand at 5576.537 kb on + strand, within Pf1N1B4_5030 at 5576.730 kb on + strand, within Pf1N1B4_5030 at 5576.731 kb on - strand, within Pf1N1B4_5030 at 5576.733 kb on + strand, within Pf1N1B4_5030 at 5576.733 kb on + strand, within Pf1N1B4_5030 at 5576.734 kb on - strand at 5576.789 kb on + strand, within Pf1N1B4_5030 at 5576.789 kb on + strand, within Pf1N1B4_5030 at 5576.790 kb on - strand, within Pf1N1B4_5030 at 5576.790 kb on - strand, within Pf1N1B4_5030 at 5576.890 kb on + strand at 5576.946 kb on - strand at 5577.375 kb on + strand, within Pf1N1B4_5031 at 5577.376 kb on - strand, within Pf1N1B4_5031 at 5577.376 kb on - strand, within Pf1N1B4_5031 at 5577.376 kb on - strand, within Pf1N1B4_5031 at 5577.462 kb on + strand, within Pf1N1B4_5031 at 5577.462 kb on + strand at 5577.462 kb on + strand, within Pf1N1B4_5031 at 5577.462 kb on + strand, within Pf1N1B4_5031 at 5577.463 kb on - strand, within Pf1N1B4_5031 at 5577.650 kb on + strand, within Pf1N1B4_5031 at 5577.868 kb on - strand, within Pf1N1B4_5031
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 5,575,256 + -0.1 5,575,256 + -2.0 5,575,412 + -0.1 5,575,429 - -0.1 5,575,431 - -0.1 5,575,431 - -0.1 5,575,778 + Pf1N1B4_5029 0.40 -0.1 5,575,820 + Pf1N1B4_5029 0.45 -4.4 5,575,820 + Pf1N1B4_5029 0.45 -2.4 5,575,820 + -0.1 5,575,821 - Pf1N1B4_5029 0.46 -3.4 5,576,049 - Pf1N1B4_5029 0.76 -0.1 5,576,138 - Pf1N1B4_5029 0.87 -0.1 5,576,177 + -0.1 5,576,231 + -4.5 5,576,232 - -1.7 5,576,426 + Pf1N1B4_5030 0.28 -0.1 5,576,427 - Pf1N1B4_5030 0.28 -1.3 5,576,427 - Pf1N1B4_5030 0.28 -0.1 5,576,427 - -0.1 5,576,537 + Pf1N1B4_5030 0.44 -2.7 5,576,730 + Pf1N1B4_5030 0.73 -0.1 5,576,731 - Pf1N1B4_5030 0.73 -4.7 5,576,733 + Pf1N1B4_5030 0.73 -0.1 5,576,733 + Pf1N1B4_5030 0.73 -2.0 5,576,734 - -3.9 5,576,789 + Pf1N1B4_5030 0.81 -4.4 5,576,789 + Pf1N1B4_5030 0.81 -5.3 5,576,790 - Pf1N1B4_5030 0.82 -3.7 5,576,790 - Pf1N1B4_5030 0.82 -4.8 5,576,890 + -0.1 5,576,946 - -0.9 5,577,375 + Pf1N1B4_5031 0.42 -0.1 5,577,376 - Pf1N1B4_5031 0.42 -2.4 5,577,376 - Pf1N1B4_5031 0.42 -0.1 5,577,376 - Pf1N1B4_5031 0.42 -0.1 5,577,462 + Pf1N1B4_5031 0.50 -0.1 5,577,462 + -0.1 5,577,462 + Pf1N1B4_5031 0.50 -0.1 5,577,462 + Pf1N1B4_5031 0.50 -1.4 5,577,463 - Pf1N1B4_5031 0.50 -0.1 5,577,650 + Pf1N1B4_5031 0.68 -0.1 5,577,868 - Pf1N1B4_5031 0.88 -4.9
Or see this region's nucleotide sequence