Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4931 and Pf1N1B4_4932 are separated by 40 nucleotides Pf1N1B4_4932 and Pf1N1B4_4933 are separated by 171 nucleotides Pf1N1B4_4933 and Pf1N1B4_4934 are separated by 224 nucleotides
Pf1N1B4_4931 - Glutarate-semialdehyde dehydrogenase (EC 1.2.1.20); Succinate-semialdehyde dehydrogenase [NADP+] (EC 1.2.1.79), at 5,465,202 to 5,466,659
_4931
Pf1N1B4_4932 - Tartrate dehydrogenase (EC 1.1.1.93) / Tartrate decarboxylase (EC 4.1.1.73) / D-malic enzyme (EC 1.1.1.83), at 5,466,700 to 5,467,779
_4932
Pf1N1B4_4933 - FIG00955097: hypothetical protein, at 5,467,951 to 5,468,175
_4933
Pf1N1B4_4934 - DNA damage response nuclease (from data) , at 5,468,400 to 5,470,052
_4934
Position (kb)
5466
5467
5468 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5466.114 kb on + strand, within Pf1N1B4_4931 at 5466.115 kb on - strand, within Pf1N1B4_4931 at 5466.457 kb on - strand at 5466.460 kb on - strand, within Pf1N1B4_4931 at 5466.460 kb on - strand, within Pf1N1B4_4931 at 5466.606 kb on + strand at 5466.622 kb on - strand at 5466.646 kb on + strand at 5466.647 kb on - strand at 5466.647 kb on - strand at 5466.824 kb on + strand, within Pf1N1B4_4932 at 5466.847 kb on + strand at 5467.006 kb on + strand, within Pf1N1B4_4932 at 5467.117 kb on + strand, within Pf1N1B4_4932 at 5467.567 kb on + strand, within Pf1N1B4_4932 at 5467.567 kb on + strand, within Pf1N1B4_4932 at 5467.568 kb on - strand, within Pf1N1B4_4932 at 5467.926 kb on - strand at 5468.072 kb on + strand at 5468.175 kb on + strand at 5468.176 kb on - strand at 5468.347 kb on - strand at 5468.347 kb on - strand at 5468.347 kb on - strand at 5468.353 kb on + strand at 5468.408 kb on - strand at 5468.408 kb on - strand at 5468.488 kb on + strand at 5468.610 kb on + strand, within Pf1N1B4_4934 at 5468.611 kb on - strand, within Pf1N1B4_4934
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 5,466,114 + Pf1N1B4_4931 0.63 -0.1 5,466,115 - Pf1N1B4_4931 0.63 -2.4 5,466,457 - -0.1 5,466,460 - Pf1N1B4_4931 0.86 -0.1 5,466,460 - Pf1N1B4_4931 0.86 -1.0 5,466,606 + -0.1 5,466,622 - -1.9 5,466,646 + +0.5 5,466,647 - -1.0 5,466,647 - -0.1 5,466,824 + Pf1N1B4_4932 0.11 -3.4 5,466,847 + -0.1 5,467,006 + Pf1N1B4_4932 0.28 -0.1 5,467,117 + Pf1N1B4_4932 0.39 -0.1 5,467,567 + Pf1N1B4_4932 0.80 -0.1 5,467,567 + Pf1N1B4_4932 0.80 -2.2 5,467,568 - Pf1N1B4_4932 0.80 -2.0 5,467,926 - -0.1 5,468,072 + -2.2 5,468,175 + -2.0 5,468,176 - -2.5 5,468,347 - -0.1 5,468,347 - -0.1 5,468,347 - -0.1 5,468,353 + -0.9 5,468,408 - -0.1 5,468,408 - -1.5 5,468,488 + -0.1 5,468,610 + Pf1N1B4_4934 0.13 -0.1 5,468,611 - Pf1N1B4_4934 0.13 -0.1
Or see this region's nucleotide sequence