Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4926

Experiment: Nitrate reduction

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4924 and Pf1N1B4_4925 overlap by 4 nucleotidesPf1N1B4_4925 and Pf1N1B4_4926 are separated by 1 nucleotidesPf1N1B4_4926 and Pf1N1B4_4927 overlap by 4 nucleotidesPf1N1B4_4927 and Pf1N1B4_4928 overlap by 19 nucleotides Pf1N1B4_4924 - Peptide ABC transporter, permease protein, at 5,457,280 to 5,458,236 _4924 Pf1N1B4_4925 - Dipeptide transport system permease protein dppC, at 5,458,233 to 5,459,108 _4925 Pf1N1B4_4926 - ATPase components of various ABC-type transport systems, contain duplicated ATPase, at 5,459,110 to 5,460,951 _4926 Pf1N1B4_4927 - Hypothetical, similar to sarcosine oxidase alpha subunit, 2Fe-2S domain, at 5,460,948 to 5,461,256 _4927 Pf1N1B4_4928 - Ferredoxin reductase, at 5,461,238 to 5,462,629 _4928 Position (kb) 5459 5460 5461Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 5458.776 kb on + strand, within Pf1N1B4_4925at 5458.777 kb on - strand, within Pf1N1B4_4925at 5459.062 kb on + strandat 5459.737 kb on + strandat 5459.857 kb on + strand, within Pf1N1B4_4926at 5459.858 kb on - strand, within Pf1N1B4_4926at 5459.858 kb on - strand, within Pf1N1B4_4926at 5459.980 kb on - strand, within Pf1N1B4_4926at 5460.280 kb on + strand, within Pf1N1B4_4926at 5460.280 kb on + strand, within Pf1N1B4_4926at 5460.280 kb on + strand, within Pf1N1B4_4926at 5460.281 kb on - strand, within Pf1N1B4_4926at 5460.281 kb on - strand, within Pf1N1B4_4926at 5460.281 kb on - strand, within Pf1N1B4_4926at 5460.326 kb on + strandat 5460.327 kb on - strand, within Pf1N1B4_4926at 5460.327 kb on - strand, within Pf1N1B4_4926at 5460.538 kb on + strand, within Pf1N1B4_4926at 5460.601 kb on + strandat 5460.665 kb on - strand, within Pf1N1B4_4926at 5460.665 kb on - strand, within Pf1N1B4_4926at 5460.665 kb on - strand, within Pf1N1B4_4926at 5461.379 kb on + strand, within Pf1N1B4_4928at 5461.402 kb on + strand, within Pf1N1B4_4928at 5461.412 kb on + strandat 5461.425 kb on - strand, within Pf1N1B4_4928at 5461.649 kb on + strand, within Pf1N1B4_4928at 5461.649 kb on + strand, within Pf1N1B4_4928at 5461.649 kb on + strand, within Pf1N1B4_4928at 5461.649 kb on + strand, within Pf1N1B4_4928at 5461.650 kb on - strand, within Pf1N1B4_4928at 5461.650 kb on - strandat 5461.739 kb on + strand, within Pf1N1B4_4928at 5461.740 kb on - strand, within Pf1N1B4_4928at 5461.757 kb on + strand, within Pf1N1B4_4928at 5461.935 kb on + strand, within Pf1N1B4_4928

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction
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5,458,776 + Pf1N1B4_4925 0.62 -0.1
5,458,777 - Pf1N1B4_4925 0.62 -0.1
5,459,062 + -2.1
5,459,737 + -0.1
5,459,857 + Pf1N1B4_4926 0.41 -1.9
5,459,858 - Pf1N1B4_4926 0.41 -0.1
5,459,858 - Pf1N1B4_4926 0.41 -0.1
5,459,980 - Pf1N1B4_4926 0.47 -0.1
5,460,280 + Pf1N1B4_4926 0.64 -3.0
5,460,280 + Pf1N1B4_4926 0.64 -2.8
5,460,280 + Pf1N1B4_4926 0.64 -0.1
5,460,281 - Pf1N1B4_4926 0.64 -0.1
5,460,281 - Pf1N1B4_4926 0.64 -0.1
5,460,281 - Pf1N1B4_4926 0.64 -2.6
5,460,326 + -2.8
5,460,327 - Pf1N1B4_4926 0.66 -0.1
5,460,327 - Pf1N1B4_4926 0.66 -2.0
5,460,538 + Pf1N1B4_4926 0.78 -3.8
5,460,601 + -1.5
5,460,665 - Pf1N1B4_4926 0.84 -0.1
5,460,665 - Pf1N1B4_4926 0.84 -0.1
5,460,665 - Pf1N1B4_4926 0.84 -0.1
5,461,379 + Pf1N1B4_4928 0.10 -2.0
5,461,402 + Pf1N1B4_4928 0.12 -0.1
5,461,412 + -1.4
5,461,425 - Pf1N1B4_4928 0.13 -1.9
5,461,649 + Pf1N1B4_4928 0.30 -0.1
5,461,649 + Pf1N1B4_4928 0.30 -0.1
5,461,649 + Pf1N1B4_4928 0.30 -0.1
5,461,649 + Pf1N1B4_4928 0.30 -1.6
5,461,650 - Pf1N1B4_4928 0.30 -3.1
5,461,650 - -1.2
5,461,739 + Pf1N1B4_4928 0.36 -0.1
5,461,740 - Pf1N1B4_4928 0.36 -0.8
5,461,757 + Pf1N1B4_4928 0.37 -2.4
5,461,935 + Pf1N1B4_4928 0.50 -0.1

Or see this region's nucleotide sequence