Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_447 and Pf1N1B4_448 are separated by 3 nucleotides Pf1N1B4_448 and Pf1N1B4_449 overlap by 1 nucleotides Pf1N1B4_449 and Pf1N1B4_450 are separated by 144 nucleotides Pf1N1B4_450 and Pf1N1B4_451 are separated by 191 nucleotides
Pf1N1B4_447 - FIG004453: protein YceG like, at 538,996 to 540,210
_447
Pf1N1B4_448 - Aminodeoxychorismate lyase (EC 4.1.3.38), at 540,214 to 541,029
_448
Pf1N1B4_449 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.179), at 541,029 to 542,237
_449
Pf1N1B4_450 - Acyl carrier protein, at 542,382 to 542,618
_450
Pf1N1B4_451 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 542,810 to 543,553
_451
Position (kb) 541
542
543 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 540.052 kb on - strand, within Pf1N1B4_447 at 540.052 kb on - strand, within Pf1N1B4_447 at 540.201 kb on + strand at 540.202 kb on - strand at 540.202 kb on - strand at 540.202 kb on - strand at 540.252 kb on + strand at 540.290 kb on + strand at 540.314 kb on + strand, within Pf1N1B4_448 at 540.416 kb on - strand at 540.498 kb on + strand, within Pf1N1B4_448 at 540.821 kb on + strand, within Pf1N1B4_448 at 541.802 kb on - strand, within Pf1N1B4_449 at 542.668 kb on - strand at 542.676 kb on - strand at 542.812 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 540,052 - Pf1N1B4_447 0.87 -2.3 540,052 - Pf1N1B4_447 0.87 -2.5 540,201 + -2.6 540,202 - -0.0 540,202 - -1.3 540,202 - -2.0 540,252 + -0.0 540,290 + -1.8 540,314 + Pf1N1B4_448 0.12 -0.0 540,416 - -4.4 540,498 + Pf1N1B4_448 0.35 -3.6 540,821 + Pf1N1B4_448 0.74 -0.0 541,802 - Pf1N1B4_449 0.64 -0.0 542,668 - -0.0 542,676 - -0.0 542,812 - -0.0
Or see this region's nucleotide sequence