Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4477

Experiment: Nitrate reduction

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4476 and Pf1N1B4_4477 are separated by 234 nucleotidesPf1N1B4_4477 and Pf1N1B4_4478 are separated by 18 nucleotides Pf1N1B4_4476 - Branched-chain amino acid aminotransferase (EC 2.6.1.42), at 4,936,524 to 4,937,543 _4476 Pf1N1B4_4477 - Dihydrolipoamide dehydrogenase of branched-chain alpha-keto acid dehydrogenase (EC 1.8.1.4), at 4,937,778 to 4,939,160 _4477 Pf1N1B4_4478 - Dihydrolipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.168), at 4,939,179 to 4,940,450 _4478 Position (kb) 4937 4938 4939 4940Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 4936.918 kb on + strand, within Pf1N1B4_4476at 4936.919 kb on - strand, within Pf1N1B4_4476at 4936.978 kb on + strand, within Pf1N1B4_4476at 4936.978 kb on + strand, within Pf1N1B4_4476at 4936.979 kb on - strand, within Pf1N1B4_4476at 4936.979 kb on - strand, within Pf1N1B4_4476at 4936.979 kb on - strand, within Pf1N1B4_4476at 4937.387 kb on - strand, within Pf1N1B4_4476at 4937.500 kb on + strandat 4937.501 kb on - strandat 4937.554 kb on - strandat 4937.616 kb on + strandat 4937.616 kb on + strandat 4937.616 kb on + strandat 4937.616 kb on + strandat 4937.616 kb on + strandat 4937.616 kb on + strandat 4937.617 kb on - strandat 4937.617 kb on - strandat 4937.617 kb on - strandat 4937.617 kb on - strandat 4937.617 kb on - strandat 4937.617 kb on - strandat 4937.617 kb on - strandat 4937.780 kb on - strandat 4938.254 kb on - strand, within Pf1N1B4_4477at 4938.431 kb on + strand, within Pf1N1B4_4477at 4938.431 kb on + strand, within Pf1N1B4_4477at 4938.432 kb on - strand, within Pf1N1B4_4477at 4938.533 kb on - strand, within Pf1N1B4_4477at 4938.533 kb on - strand, within Pf1N1B4_4477at 4938.619 kb on + strand, within Pf1N1B4_4477at 4939.108 kb on + strandat 4939.181 kb on - strandat 4939.787 kb on - strandat 4939.933 kb on + strand, within Pf1N1B4_4478at 4939.934 kb on - strand, within Pf1N1B4_4478at 4939.934 kb on - strand, within Pf1N1B4_4478

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction
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4,936,918 + Pf1N1B4_4476 0.39 -6.5
4,936,919 - Pf1N1B4_4476 0.39 -0.1
4,936,978 + Pf1N1B4_4476 0.45 -0.1
4,936,978 + Pf1N1B4_4476 0.45 -5.1
4,936,979 - Pf1N1B4_4476 0.45 -5.9
4,936,979 - Pf1N1B4_4476 0.45 -4.1
4,936,979 - Pf1N1B4_4476 0.45 -3.2
4,937,387 - Pf1N1B4_4476 0.85 -0.1
4,937,500 + -0.1
4,937,501 - -0.1
4,937,554 - -2.1
4,937,616 + -0.1
4,937,616 + -3.0
4,937,616 + -0.1
4,937,616 + -0.1
4,937,616 + -1.1
4,937,616 + -0.1
4,937,617 - -1.8
4,937,617 - -0.1
4,937,617 - -2.4
4,937,617 - -0.1
4,937,617 - -0.1
4,937,617 - -0.1
4,937,617 - -2.1
4,937,780 - -4.1
4,938,254 - Pf1N1B4_4477 0.34 -0.1
4,938,431 + Pf1N1B4_4477 0.47 -0.1
4,938,431 + Pf1N1B4_4477 0.47 -2.5
4,938,432 - Pf1N1B4_4477 0.47 -2.7
4,938,533 - Pf1N1B4_4477 0.55 -0.1
4,938,533 - Pf1N1B4_4477 0.55 -0.1
4,938,619 + Pf1N1B4_4477 0.61 -0.1
4,939,108 + -0.1
4,939,181 - -0.1
4,939,787 - -0.1
4,939,933 + Pf1N1B4_4478 0.59 -1.8
4,939,934 - Pf1N1B4_4478 0.59 -0.7
4,939,934 - Pf1N1B4_4478 0.59 -1.3

Or see this region's nucleotide sequence