Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4462 and Pf1N1B4_4463 are separated by 168 nucleotides Pf1N1B4_4463 and Pf1N1B4_4464 are separated by 47 nucleotides
Pf1N1B4_4462 - LysR family transcriptional regulator YbhD, at 4,923,028 to 4,923,918
_4462
Pf1N1B4_4463 - FIG00958979: hypothetical protein, at 4,924,087 to 4,925,169
_4463
Pf1N1B4_4464 - C4-dicarboxylate transport protein, at 4,925,217 to 4,926,536
_4464
Position (kb)
4924
4925
4926 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4923.258 kb on - strand, within Pf1N1B4_4462 at 4923.575 kb on + strand, within Pf1N1B4_4462 at 4923.575 kb on + strand, within Pf1N1B4_4462 at 4923.778 kb on - strand at 4923.784 kb on + strand, within Pf1N1B4_4462 at 4923.785 kb on - strand, within Pf1N1B4_4462 at 4924.021 kb on + strand at 4924.021 kb on + strand at 4924.021 kb on + strand at 4924.122 kb on + strand at 4924.140 kb on - strand at 4924.287 kb on - strand, within Pf1N1B4_4463 at 4924.309 kb on + strand at 4924.309 kb on + strand, within Pf1N1B4_4463 at 4924.310 kb on - strand at 4924.513 kb on + strand, within Pf1N1B4_4463 at 4924.513 kb on + strand, within Pf1N1B4_4463 at 4924.513 kb on + strand, within Pf1N1B4_4463 at 4924.514 kb on - strand, within Pf1N1B4_4463 at 4924.514 kb on - strand, within Pf1N1B4_4463 at 4924.756 kb on - strand, within Pf1N1B4_4463 at 4925.062 kb on + strand at 4925.063 kb on - strand at 4925.167 kb on + strand at 4925.250 kb on + strand at 4925.250 kb on + strand at 4925.250 kb on + strand at 4925.645 kb on + strand, within Pf1N1B4_4464 at 4925.647 kb on + strand, within Pf1N1B4_4464 at 4925.647 kb on + strand at 4925.647 kb on + strand, within Pf1N1B4_4464 at 4925.647 kb on + strand, within Pf1N1B4_4464 at 4925.647 kb on + strand, within Pf1N1B4_4464 at 4925.648 kb on - strand, within Pf1N1B4_4464 at 4925.648 kb on - strand, within Pf1N1B4_4464 at 4925.648 kb on - strand at 4925.793 kb on + strand, within Pf1N1B4_4464 at 4925.794 kb on - strand, within Pf1N1B4_4464 at 4925.901 kb on + strand, within Pf1N1B4_4464 at 4925.902 kb on - strand, within Pf1N1B4_4464 at 4925.968 kb on + strand, within Pf1N1B4_4464
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 4,923,258 - Pf1N1B4_4462 0.26 -0.1 4,923,575 + Pf1N1B4_4462 0.61 -0.1 4,923,575 + Pf1N1B4_4462 0.61 -0.1 4,923,778 - -0.1 4,923,784 + Pf1N1B4_4462 0.85 -1.8 4,923,785 - Pf1N1B4_4462 0.85 -2.7 4,924,021 + -2.2 4,924,021 + -0.1 4,924,021 + -0.1 4,924,122 + -0.1 4,924,140 - -2.4 4,924,287 - Pf1N1B4_4463 0.18 -1.1 4,924,309 + -1.3 4,924,309 + Pf1N1B4_4463 0.20 -0.1 4,924,310 - -1.4 4,924,513 + Pf1N1B4_4463 0.39 -2.4 4,924,513 + Pf1N1B4_4463 0.39 -0.1 4,924,513 + Pf1N1B4_4463 0.39 -0.1 4,924,514 - Pf1N1B4_4463 0.39 -3.6 4,924,514 - Pf1N1B4_4463 0.39 -2.2 4,924,756 - Pf1N1B4_4463 0.62 -2.3 4,925,062 + -0.1 4,925,063 - -0.1 4,925,167 + -0.1 4,925,250 + -2.1 4,925,250 + -0.1 4,925,250 + -2.2 4,925,645 + Pf1N1B4_4464 0.32 -1.9 4,925,647 + Pf1N1B4_4464 0.33 -1.6 4,925,647 + -0.1 4,925,647 + Pf1N1B4_4464 0.33 -0.1 4,925,647 + Pf1N1B4_4464 0.33 -2.0 4,925,647 + Pf1N1B4_4464 0.33 -0.1 4,925,648 - Pf1N1B4_4464 0.33 -0.1 4,925,648 - Pf1N1B4_4464 0.33 -0.5 4,925,648 - -1.6 4,925,793 + Pf1N1B4_4464 0.44 -0.7 4,925,794 - Pf1N1B4_4464 0.44 -0.1 4,925,901 + Pf1N1B4_4464 0.52 -0.1 4,925,902 - Pf1N1B4_4464 0.52 -1.3 4,925,968 + Pf1N1B4_4464 0.57 -0.1
Or see this region's nucleotide sequence