Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4192

Experiment: Nitrate reduction

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4191 and Pf1N1B4_4192 are separated by 77 nucleotidesPf1N1B4_4192 and Pf1N1B4_4193 are separated by 47 nucleotidesPf1N1B4_4193 and Pf1N1B4_4194 are separated by 52 nucleotides Pf1N1B4_4191 - Bifunctional protein: zinc-containing alcohol dehydrogenase; quinone oxidoreductase ( NADPH:quinone reductase) (EC 1.1.1.-); Similar to arginate lyase, at 4,643,834 to 4,644,832 _4191 Pf1N1B4_4192 - Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase, at 4,644,910 to 4,645,776 _4192 Pf1N1B4_4193 - Oxidoreductase, short chain dehydrogenase/reductase family, at 4,645,824 to 4,646,612 _4193 Pf1N1B4_4194 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 4,646,665 to 4,647,786 _4194 Position (kb) 4644 4645 4646Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 4643.981 kb on + strand, within Pf1N1B4_4191at 4643.981 kb on + strand, within Pf1N1B4_4191at 4643.981 kb on + strand, within Pf1N1B4_4191at 4644.011 kb on - strand, within Pf1N1B4_4191at 4644.182 kb on + strand, within Pf1N1B4_4191at 4644.183 kb on - strand, within Pf1N1B4_4191at 4644.252 kb on + strand, within Pf1N1B4_4191at 4644.573 kb on + strand, within Pf1N1B4_4191at 4644.574 kb on - strand, within Pf1N1B4_4191at 4644.650 kb on + strand, within Pf1N1B4_4191at 4644.650 kb on + strand, within Pf1N1B4_4191at 4644.664 kb on + strand, within Pf1N1B4_4191at 4644.664 kb on + strand, within Pf1N1B4_4191at 4644.664 kb on + strand, within Pf1N1B4_4191at 4644.665 kb on - strand, within Pf1N1B4_4191at 4644.665 kb on - strand, within Pf1N1B4_4191at 4644.665 kb on - strand, within Pf1N1B4_4191at 4644.665 kb on - strand, within Pf1N1B4_4191at 4644.665 kb on - strand, within Pf1N1B4_4191at 4644.665 kb on - strand, within Pf1N1B4_4191at 4644.667 kb on - strandat 4644.902 kb on - strandat 4644.902 kb on - strandat 4644.902 kb on - strandat 4644.989 kb on + strandat 4645.089 kb on + strand, within Pf1N1B4_4192at 4645.089 kb on + strand, within Pf1N1B4_4192at 4645.090 kb on - strand, within Pf1N1B4_4192at 4645.135 kb on - strand, within Pf1N1B4_4192at 4645.145 kb on - strand, within Pf1N1B4_4192at 4645.154 kb on - strand, within Pf1N1B4_4192at 4645.762 kb on - strandat 4645.841 kb on - strandat 4646.083 kb on + strand, within Pf1N1B4_4193at 4646.083 kb on + strand, within Pf1N1B4_4193at 4646.083 kb on + strandat 4646.084 kb on - strand, within Pf1N1B4_4193at 4646.084 kb on - strand, within Pf1N1B4_4193at 4646.084 kb on - strand, within Pf1N1B4_4193at 4646.084 kb on - strand, within Pf1N1B4_4193at 4646.166 kb on - strand, within Pf1N1B4_4193at 4646.598 kb on - strandat 4646.732 kb on + strandat 4646.733 kb on - strandat 4646.733 kb on - strandat 4646.753 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction
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4,643,981 + Pf1N1B4_4191 0.15 -3.5
4,643,981 + Pf1N1B4_4191 0.15 -0.6
4,643,981 + Pf1N1B4_4191 0.15 -0.1
4,644,011 - Pf1N1B4_4191 0.18 -1.8
4,644,182 + Pf1N1B4_4191 0.35 -1.8
4,644,183 - Pf1N1B4_4191 0.35 -2.3
4,644,252 + Pf1N1B4_4191 0.42 -0.1
4,644,573 + Pf1N1B4_4191 0.74 -0.1
4,644,574 - Pf1N1B4_4191 0.74 -0.1
4,644,650 + Pf1N1B4_4191 0.82 -1.5
4,644,650 + Pf1N1B4_4191 0.82 -0.1
4,644,664 + Pf1N1B4_4191 0.83 -2.8
4,644,664 + Pf1N1B4_4191 0.83 -0.1
4,644,664 + Pf1N1B4_4191 0.83 -0.5
4,644,665 - Pf1N1B4_4191 0.83 -0.1
4,644,665 - Pf1N1B4_4191 0.83 -0.8
4,644,665 - Pf1N1B4_4191 0.83 -0.1
4,644,665 - Pf1N1B4_4191 0.83 -1.4
4,644,665 - Pf1N1B4_4191 0.83 -1.5
4,644,665 - Pf1N1B4_4191 0.83 -0.9
4,644,667 - -0.1
4,644,902 - -0.1
4,644,902 - -0.1
4,644,902 - -0.8
4,644,989 + -0.1
4,645,089 + Pf1N1B4_4192 0.21 -2.1
4,645,089 + Pf1N1B4_4192 0.21 -0.1
4,645,090 - Pf1N1B4_4192 0.21 -0.1
4,645,135 - Pf1N1B4_4192 0.26 -3.1
4,645,145 - Pf1N1B4_4192 0.27 -0.1
4,645,154 - Pf1N1B4_4192 0.28 -0.1
4,645,762 - -0.1
4,645,841 - -0.1
4,646,083 + Pf1N1B4_4193 0.33 -0.1
4,646,083 + Pf1N1B4_4193 0.33 -0.1
4,646,083 + -1.1
4,646,084 - Pf1N1B4_4193 0.33 -0.1
4,646,084 - Pf1N1B4_4193 0.33 -0.1
4,646,084 - Pf1N1B4_4193 0.33 -0.1
4,646,084 - Pf1N1B4_4193 0.33 -2.2
4,646,166 - Pf1N1B4_4193 0.43 -0.1
4,646,598 - -4.8
4,646,732 + -0.1
4,646,733 - -0.1
4,646,733 - -1.5
4,646,753 + -0.1

Or see this region's nucleotide sequence