Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4191 and Pf1N1B4_4192 are separated by 77 nucleotides Pf1N1B4_4192 and Pf1N1B4_4193 are separated by 47 nucleotides Pf1N1B4_4193 and Pf1N1B4_4194 are separated by 52 nucleotides
Pf1N1B4_4191 - Bifunctional protein: zinc-containing alcohol dehydrogenase; quinone oxidoreductase ( NADPH:quinone reductase) (EC 1.1.1.-); Similar to arginate lyase, at 4,643,834 to 4,644,832
_4191
Pf1N1B4_4192 - Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase, at 4,644,910 to 4,645,776
_4192
Pf1N1B4_4193 - Oxidoreductase, short chain dehydrogenase/reductase family, at 4,645,824 to 4,646,612
_4193
Pf1N1B4_4194 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 4,646,665 to 4,647,786
_4194
Position (kb)
4644
4645
4646 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4643.981 kb on + strand, within Pf1N1B4_4191 at 4643.981 kb on + strand, within Pf1N1B4_4191 at 4643.981 kb on + strand, within Pf1N1B4_4191 at 4644.011 kb on - strand, within Pf1N1B4_4191 at 4644.182 kb on + strand, within Pf1N1B4_4191 at 4644.183 kb on - strand, within Pf1N1B4_4191 at 4644.252 kb on + strand, within Pf1N1B4_4191 at 4644.573 kb on + strand, within Pf1N1B4_4191 at 4644.574 kb on - strand, within Pf1N1B4_4191 at 4644.650 kb on + strand, within Pf1N1B4_4191 at 4644.650 kb on + strand, within Pf1N1B4_4191 at 4644.664 kb on + strand, within Pf1N1B4_4191 at 4644.664 kb on + strand, within Pf1N1B4_4191 at 4644.664 kb on + strand, within Pf1N1B4_4191 at 4644.665 kb on - strand, within Pf1N1B4_4191 at 4644.665 kb on - strand, within Pf1N1B4_4191 at 4644.665 kb on - strand, within Pf1N1B4_4191 at 4644.665 kb on - strand, within Pf1N1B4_4191 at 4644.665 kb on - strand, within Pf1N1B4_4191 at 4644.665 kb on - strand, within Pf1N1B4_4191 at 4644.667 kb on - strand at 4644.902 kb on - strand at 4644.902 kb on - strand at 4644.902 kb on - strand at 4644.989 kb on + strand at 4645.089 kb on + strand, within Pf1N1B4_4192 at 4645.089 kb on + strand, within Pf1N1B4_4192 at 4645.090 kb on - strand, within Pf1N1B4_4192 at 4645.135 kb on - strand, within Pf1N1B4_4192 at 4645.145 kb on - strand, within Pf1N1B4_4192 at 4645.154 kb on - strand, within Pf1N1B4_4192 at 4645.762 kb on - strand at 4645.841 kb on - strand at 4646.083 kb on + strand, within Pf1N1B4_4193 at 4646.083 kb on + strand, within Pf1N1B4_4193 at 4646.083 kb on + strand at 4646.084 kb on - strand, within Pf1N1B4_4193 at 4646.084 kb on - strand, within Pf1N1B4_4193 at 4646.084 kb on - strand, within Pf1N1B4_4193 at 4646.084 kb on - strand, within Pf1N1B4_4193 at 4646.166 kb on - strand, within Pf1N1B4_4193 at 4646.598 kb on - strand at 4646.732 kb on + strand at 4646.733 kb on - strand at 4646.733 kb on - strand at 4646.753 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 4,643,981 + Pf1N1B4_4191 0.15 -3.5 4,643,981 + Pf1N1B4_4191 0.15 -0.6 4,643,981 + Pf1N1B4_4191 0.15 -0.1 4,644,011 - Pf1N1B4_4191 0.18 -1.8 4,644,182 + Pf1N1B4_4191 0.35 -1.8 4,644,183 - Pf1N1B4_4191 0.35 -2.3 4,644,252 + Pf1N1B4_4191 0.42 -0.1 4,644,573 + Pf1N1B4_4191 0.74 -0.1 4,644,574 - Pf1N1B4_4191 0.74 -0.1 4,644,650 + Pf1N1B4_4191 0.82 -1.5 4,644,650 + Pf1N1B4_4191 0.82 -0.1 4,644,664 + Pf1N1B4_4191 0.83 -2.8 4,644,664 + Pf1N1B4_4191 0.83 -0.1 4,644,664 + Pf1N1B4_4191 0.83 -0.5 4,644,665 - Pf1N1B4_4191 0.83 -0.1 4,644,665 - Pf1N1B4_4191 0.83 -0.8 4,644,665 - Pf1N1B4_4191 0.83 -0.1 4,644,665 - Pf1N1B4_4191 0.83 -1.4 4,644,665 - Pf1N1B4_4191 0.83 -1.5 4,644,665 - Pf1N1B4_4191 0.83 -0.9 4,644,667 - -0.1 4,644,902 - -0.1 4,644,902 - -0.1 4,644,902 - -0.8 4,644,989 + -0.1 4,645,089 + Pf1N1B4_4192 0.21 -2.1 4,645,089 + Pf1N1B4_4192 0.21 -0.1 4,645,090 - Pf1N1B4_4192 0.21 -0.1 4,645,135 - Pf1N1B4_4192 0.26 -3.1 4,645,145 - Pf1N1B4_4192 0.27 -0.1 4,645,154 - Pf1N1B4_4192 0.28 -0.1 4,645,762 - -0.1 4,645,841 - -0.1 4,646,083 + Pf1N1B4_4193 0.33 -0.1 4,646,083 + Pf1N1B4_4193 0.33 -0.1 4,646,083 + -1.1 4,646,084 - Pf1N1B4_4193 0.33 -0.1 4,646,084 - Pf1N1B4_4193 0.33 -0.1 4,646,084 - Pf1N1B4_4193 0.33 -0.1 4,646,084 - Pf1N1B4_4193 0.33 -2.2 4,646,166 - Pf1N1B4_4193 0.43 -0.1 4,646,598 - -4.8 4,646,732 + -0.1 4,646,733 - -0.1 4,646,733 - -1.5 4,646,753 + -0.1
Or see this region's nucleotide sequence