Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3781 and Pf1N1B4_3782 are separated by 160 nucleotides Pf1N1B4_3782 and Pf1N1B4_3783 are separated by 235 nucleotides Pf1N1B4_3783 and Pf1N1B4_3784 are separated by 354 nucleotides
Pf1N1B4_3781 - Nucleoside:H+ symporter:Major facilitator superfamily, at 4,190,320 to 4,191,465
_3781
Pf1N1B4_3782 - 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (EC 1.13.11.54), at 4,191,626 to 4,192,171
_3782
Pf1N1B4_3783 - FIG00958237: hypothetical protein, at 4,192,407 to 4,192,685
_3783
Pf1N1B4_3784 - Acetoacetyl-CoA synthetase (EC 6.2.1.16) / Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 4,193,040 to 4,194,878
_3784
Position (kb)
4192
4193 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4191.444 kb on + strand at 4191.444 kb on + strand at 4191.444 kb on + strand at 4191.444 kb on + strand at 4191.444 kb on + strand at 4191.444 kb on + strand at 4191.444 kb on + strand at 4191.444 kb on + strand at 4191.444 kb on + strand at 4191.445 kb on - strand at 4191.445 kb on - strand at 4191.445 kb on - strand at 4191.445 kb on - strand at 4191.445 kb on - strand at 4191.445 kb on - strand at 4191.445 kb on - strand at 4191.445 kb on - strand at 4191.572 kb on + strand at 4191.597 kb on - strand at 4191.666 kb on + strand at 4191.666 kb on + strand at 4191.936 kb on + strand, within Pf1N1B4_3782 at 4191.937 kb on - strand, within Pf1N1B4_3782 at 4191.986 kb on + strand, within Pf1N1B4_3782 at 4191.986 kb on + strand, within Pf1N1B4_3782 at 4191.986 kb on + strand at 4191.987 kb on - strand, within Pf1N1B4_3782 at 4192.120 kb on + strand at 4192.120 kb on + strand at 4192.121 kb on - strand at 4192.174 kb on - strand at 4192.174 kb on - strand at 4192.207 kb on + strand at 4192.208 kb on - strand at 4192.208 kb on - strand at 4192.216 kb on + strand at 4192.217 kb on - strand at 4192.217 kb on - strand at 4192.217 kb on - strand at 4192.217 kb on - strand at 4192.217 kb on - strand at 4192.217 kb on - strand at 4192.224 kb on - strand at 4192.309 kb on + strand at 4192.309 kb on + strand at 4192.309 kb on + strand at 4192.309 kb on + strand at 4192.309 kb on + strand at 4192.309 kb on + strand at 4192.309 kb on + strand at 4192.378 kb on + strand at 4192.379 kb on - strand at 4192.387 kb on + strand at 4192.388 kb on - strand at 4192.388 kb on - strand at 4192.388 kb on - strand at 4192.388 kb on - strand at 4192.388 kb on - strand at 4192.391 kb on + strand at 4192.391 kb on + strand at 4192.392 kb on - strand at 4192.392 kb on - strand at 4192.394 kb on - strand at 4192.394 kb on - strand at 4192.394 kb on - strand at 4192.394 kb on - strand at 4192.420 kb on + strand at 4192.420 kb on + strand at 4192.420 kb on + strand at 4192.420 kb on + strand at 4192.420 kb on + strand at 4192.420 kb on + strand at 4192.421 kb on - strand at 4192.504 kb on - strand, within Pf1N1B4_3783 at 4192.504 kb on - strand, within Pf1N1B4_3783 at 4192.553 kb on - strand, within Pf1N1B4_3783 at 4192.653 kb on + strand, within Pf1N1B4_3783 at 4192.962 kb on + strand at 4192.962 kb on + strand at 4192.984 kb on + strand at 4192.984 kb on + strand at 4192.985 kb on - strand at 4193.245 kb on + strand, within Pf1N1B4_3784 at 4193.253 kb on + strand, within Pf1N1B4_3784 at 4193.253 kb on + strand, within Pf1N1B4_3784 at 4193.254 kb on - strand, within Pf1N1B4_3784 at 4193.415 kb on - strand, within Pf1N1B4_3784 at 4193.504 kb on + strand, within Pf1N1B4_3784 at 4193.505 kb on - strand, within Pf1N1B4_3784
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 4,191,444 + -2.7 4,191,444 + -0.0 4,191,444 + -0.0 4,191,444 + -0.0 4,191,444 + -2.0 4,191,444 + -2.3 4,191,444 + -0.0 4,191,444 + -2.1 4,191,444 + -0.0 4,191,445 - -1.4 4,191,445 - -1.9 4,191,445 - -0.0 4,191,445 - -0.0 4,191,445 - -0.0 4,191,445 - -0.0 4,191,445 - -0.0 4,191,445 - -0.0 4,191,572 + -0.0 4,191,597 - -2.1 4,191,666 + -0.0 4,191,666 + -2.1 4,191,936 + Pf1N1B4_3782 0.57 -0.0 4,191,937 - Pf1N1B4_3782 0.57 -0.0 4,191,986 + Pf1N1B4_3782 0.66 -0.0 4,191,986 + Pf1N1B4_3782 0.66 -0.0 4,191,986 + -0.0 4,191,987 - Pf1N1B4_3782 0.66 -0.0 4,192,120 + -0.0 4,192,120 + -1.0 4,192,121 - -1.3 4,192,174 - -0.0 4,192,174 - -1.6 4,192,207 + -0.0 4,192,208 - -0.0 4,192,208 - -0.0 4,192,216 + -1.9 4,192,217 - -0.0 4,192,217 - -1.7 4,192,217 - -2.0 4,192,217 - -1.7 4,192,217 - -1.3 4,192,217 - -1.7 4,192,224 - -1.9 4,192,309 + -0.0 4,192,309 + -0.0 4,192,309 + -0.0 4,192,309 + -0.0 4,192,309 + -3.0 4,192,309 + -0.0 4,192,309 + -0.0 4,192,378 + -0.0 4,192,379 - -0.0 4,192,387 + -0.0 4,192,388 - -1.1 4,192,388 - -0.0 4,192,388 - -0.0 4,192,388 - -0.0 4,192,388 - -0.0 4,192,391 + -0.0 4,192,391 + -0.0 4,192,392 - -0.0 4,192,392 - -0.0 4,192,394 - -2.6 4,192,394 - -0.0 4,192,394 - -0.0 4,192,394 - -0.0 4,192,420 + -1.5 4,192,420 + -0.0 4,192,420 + -0.0 4,192,420 + -0.0 4,192,420 + -2.0 4,192,420 + -3.0 4,192,421 - -1.5 4,192,504 - Pf1N1B4_3783 0.35 -0.0 4,192,504 - Pf1N1B4_3783 0.35 -1.9 4,192,553 - Pf1N1B4_3783 0.52 -0.0 4,192,653 + Pf1N1B4_3783 0.88 -3.8 4,192,962 + -2.7 4,192,962 + -1.4 4,192,984 + -1.4 4,192,984 + -1.3 4,192,985 - -0.0 4,193,245 + Pf1N1B4_3784 0.11 -0.0 4,193,253 + Pf1N1B4_3784 0.12 -0.9 4,193,253 + Pf1N1B4_3784 0.12 -2.4 4,193,254 - Pf1N1B4_3784 0.12 -1.0 4,193,415 - Pf1N1B4_3784 0.20 -0.0 4,193,504 + Pf1N1B4_3784 0.25 -0.1 4,193,505 - Pf1N1B4_3784 0.25 -1.8
Or see this region's nucleotide sequence