Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_364

Experiment: Nitrate reduction

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_362 and Pf1N1B4_363 overlap by 8 nucleotidesPf1N1B4_363 and Pf1N1B4_364 are separated by 3 nucleotidesPf1N1B4_364 and Pf1N1B4_365 are separated by 286 nucleotidesPf1N1B4_365 and Pf1N1B4_366 are separated by 12 nucleotides Pf1N1B4_362 - Lipid A export ATP-binding/permease protein MsbA, at 430,596 to 432,413 _362 Pf1N1B4_363 - Thioredoxin, at 432,406 to 433,044 _363 Pf1N1B4_364 - Rhodanese domain protein UPF0176, at 433,048 to 433,989 _364 Pf1N1B4_365 - Cell division protein BolA, at 434,276 to 434,575 _365 Pf1N1B4_366 - FIG00954136: hypothetical protein, at 434,588 to 435,109 _366 Position (kb) 433 434Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 432.154 kb on + strandat 432.471 kb on - strand, within Pf1N1B4_363at 432.501 kb on + strand, within Pf1N1B4_363at 432.502 kb on - strand, within Pf1N1B4_363at 432.687 kb on - strand, within Pf1N1B4_363at 432.687 kb on - strand, within Pf1N1B4_363at 432.782 kb on + strand, within Pf1N1B4_363at 432.865 kb on - strand, within Pf1N1B4_363at 432.867 kb on - strand, within Pf1N1B4_363at 432.867 kb on - strand, within Pf1N1B4_363at 432.867 kb on - strand, within Pf1N1B4_363at 432.937 kb on + strand, within Pf1N1B4_363at 433.022 kb on + strandat 433.022 kb on + strandat 433.022 kb on + strandat 433.023 kb on - strandat 433.023 kb on - strandat 433.023 kb on - strandat 433.023 kb on - strandat 433.023 kb on - strandat 433.023 kb on - strandat 433.073 kb on + strandat 433.074 kb on - strandat 433.074 kb on - strandat 433.074 kb on - strandat 433.074 kb on - strandat 433.074 kb on - strandat 433.079 kb on + strandat 433.079 kb on + strandat 433.080 kb on - strandat 433.205 kb on + strand, within Pf1N1B4_364at 433.205 kb on + strand, within Pf1N1B4_364at 433.206 kb on - strand, within Pf1N1B4_364at 433.206 kb on - strand, within Pf1N1B4_364at 433.206 kb on - strand, within Pf1N1B4_364at 433.392 kb on - strand, within Pf1N1B4_364at 433.421 kb on + strand, within Pf1N1B4_364at 433.509 kb on - strand, within Pf1N1B4_364at 433.652 kb on + strand, within Pf1N1B4_364at 433.653 kb on - strand, within Pf1N1B4_364at 433.653 kb on - strand, within Pf1N1B4_364at 433.934 kb on + strandat 433.956 kb on + strandat 433.956 kb on + strandat 433.958 kb on + strandat 433.958 kb on + strandat 433.959 kb on - strandat 433.994 kb on - strandat 433.994 kb on - strandat 434.017 kb on + strandat 434.018 kb on - strandat 434.076 kb on - strandat 434.204 kb on - strandat 434.204 kb on - strandat 434.204 kb on - strandat 434.277 kb on + strandat 434.277 kb on + strandat 434.277 kb on + strandat 434.278 kb on - strandat 434.349 kb on + strand, within Pf1N1B4_365at 434.349 kb on + strand, within Pf1N1B4_365at 434.349 kb on + strand, within Pf1N1B4_365at 434.350 kb on - strand, within Pf1N1B4_365at 434.350 kb on - strand, within Pf1N1B4_365at 434.350 kb on - strand, within Pf1N1B4_365at 434.350 kb on - strand, within Pf1N1B4_365at 434.350 kb on - strand, within Pf1N1B4_365at 434.350 kb on - strand, within Pf1N1B4_365at 434.350 kb on - strand, within Pf1N1B4_365at 434.350 kb on - strand, within Pf1N1B4_365at 434.350 kb on - strand, within Pf1N1B4_365at 434.350 kb on - strand, within Pf1N1B4_365at 434.350 kb on - strand, within Pf1N1B4_365at 434.350 kb on - strand, within Pf1N1B4_365at 434.350 kb on - strand, within Pf1N1B4_365at 434.403 kb on + strand, within Pf1N1B4_365at 434.404 kb on - strand, within Pf1N1B4_365at 434.404 kb on - strand, within Pf1N1B4_365at 434.590 kb on - strandat 434.620 kb on + strandat 434.761 kb on + strand, within Pf1N1B4_366at 434.763 kb on + strand, within Pf1N1B4_366at 434.763 kb on + strand, within Pf1N1B4_366at 434.763 kb on + strand, within Pf1N1B4_366at 434.763 kb on + strand, within Pf1N1B4_366at 434.764 kb on - strand, within Pf1N1B4_366at 434.764 kb on - strand, within Pf1N1B4_366at 434.811 kb on + strand, within Pf1N1B4_366at 434.811 kb on + strand, within Pf1N1B4_366at 434.811 kb on + strand, within Pf1N1B4_366at 434.811 kb on + strand, within Pf1N1B4_366at 434.812 kb on - strand, within Pf1N1B4_366at 434.812 kb on - strand, within Pf1N1B4_366at 434.812 kb on - strand, within Pf1N1B4_366at 434.812 kb on - strand, within Pf1N1B4_366at 434.812 kb on - strand, within Pf1N1B4_366at 434.812 kb on - strand, within Pf1N1B4_366at 434.886 kb on + strand, within Pf1N1B4_366at 434.886 kb on + strandat 434.886 kb on + strand, within Pf1N1B4_366at 434.886 kb on + strand, within Pf1N1B4_366at 434.886 kb on + strand, within Pf1N1B4_366at 434.886 kb on + strandat 434.887 kb on - strand, within Pf1N1B4_366at 434.887 kb on - strand, within Pf1N1B4_366at 434.887 kb on - strand, within Pf1N1B4_366at 434.887 kb on - strand, within Pf1N1B4_366at 434.887 kb on - strand, within Pf1N1B4_366at 434.887 kb on - strand, within Pf1N1B4_366at 434.887 kb on - strand, within Pf1N1B4_366at 434.887 kb on - strand, within Pf1N1B4_366at 434.967 kb on + strand, within Pf1N1B4_366at 434.967 kb on + strand, within Pf1N1B4_366at 434.967 kb on + strand, within Pf1N1B4_366at 434.967 kb on + strand, within Pf1N1B4_366at 434.967 kb on + strand, within Pf1N1B4_366at 434.967 kb on + strand, within Pf1N1B4_366at 434.967 kb on + strand, within Pf1N1B4_366at 434.967 kb on + strand, within Pf1N1B4_366at 434.967 kb on + strand, within Pf1N1B4_366at 434.967 kb on + strand, within Pf1N1B4_366at 434.967 kb on + strand, within Pf1N1B4_366at 434.967 kb on + strand, within Pf1N1B4_366at 434.968 kb on - strand, within Pf1N1B4_366at 434.968 kb on - strand, within Pf1N1B4_366at 434.968 kb on - strand, within Pf1N1B4_366at 434.968 kb on - strand, within Pf1N1B4_366

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction
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432,154 + -0.0
432,471 - Pf1N1B4_363 0.10 -0.0
432,501 + Pf1N1B4_363 0.15 -1.2
432,502 - Pf1N1B4_363 0.15 -0.0
432,687 - Pf1N1B4_363 0.44 -0.8
432,687 - Pf1N1B4_363 0.44 -0.0
432,782 + Pf1N1B4_363 0.59 -2.4
432,865 - Pf1N1B4_363 0.72 -1.0
432,867 - Pf1N1B4_363 0.72 -0.0
432,867 - Pf1N1B4_363 0.72 -0.0
432,867 - Pf1N1B4_363 0.72 -2.1
432,937 + Pf1N1B4_363 0.83 -0.0
433,022 + -0.0
433,022 + -0.0
433,022 + -0.0
433,023 - -0.7
433,023 - -0.0
433,023 - -0.0
433,023 - -0.0
433,023 - -0.0
433,023 - -0.0
433,073 + -1.9
433,074 - -0.0
433,074 - -0.0
433,074 - -0.0
433,074 - -0.0
433,074 - -0.0
433,079 + -0.0
433,079 + -1.0
433,080 - -0.0
433,205 + Pf1N1B4_364 0.17 -0.0
433,205 + Pf1N1B4_364 0.17 -1.6
433,206 - Pf1N1B4_364 0.17 -3.0
433,206 - Pf1N1B4_364 0.17 -0.0
433,206 - Pf1N1B4_364 0.17 -3.7
433,392 - Pf1N1B4_364 0.37 -2.0
433,421 + Pf1N1B4_364 0.40 -0.0
433,509 - Pf1N1B4_364 0.49 -0.0
433,652 + Pf1N1B4_364 0.64 -0.0
433,653 - Pf1N1B4_364 0.64 -0.0
433,653 - Pf1N1B4_364 0.64 -3.3
433,934 + -0.0
433,956 + -0.0
433,956 + -0.0
433,958 + -0.0
433,958 + -0.0
433,959 - -0.0
433,994 - -2.1
433,994 - -0.0
434,017 + -2.1
434,018 - -0.0
434,076 - -0.6
434,204 - -0.0
434,204 - -0.0
434,204 - -2.8
434,277 + -0.0
434,277 + -4.3
434,277 + -1.6
434,278 - -2.0
434,349 + Pf1N1B4_365 0.24 -0.0
434,349 + Pf1N1B4_365 0.24 -1.9
434,349 + Pf1N1B4_365 0.24 -0.0
434,350 - Pf1N1B4_365 0.25 -0.0
434,350 - Pf1N1B4_365 0.25 -2.3
434,350 - Pf1N1B4_365 0.25 -3.0
434,350 - Pf1N1B4_365 0.25 -0.0
434,350 - Pf1N1B4_365 0.25 -0.0
434,350 - Pf1N1B4_365 0.25 -0.0
434,350 - Pf1N1B4_365 0.25 -0.0
434,350 - Pf1N1B4_365 0.25 -0.0
434,350 - Pf1N1B4_365 0.25 -2.5
434,350 - Pf1N1B4_365 0.25 -0.0
434,350 - Pf1N1B4_365 0.25 -3.6
434,350 - Pf1N1B4_365 0.25 -0.0
434,350 - Pf1N1B4_365 0.25 -0.0
434,403 + Pf1N1B4_365 0.42 -0.0
434,404 - Pf1N1B4_365 0.43 -0.0
434,404 - Pf1N1B4_365 0.43 -2.0
434,590 - -0.0
434,620 + -0.0
434,761 + Pf1N1B4_366 0.33 -0.0
434,763 + Pf1N1B4_366 0.34 -0.0
434,763 + Pf1N1B4_366 0.34 -0.0
434,763 + Pf1N1B4_366 0.34 -0.0
434,763 + Pf1N1B4_366 0.34 -0.0
434,764 - Pf1N1B4_366 0.34 -0.0
434,764 - Pf1N1B4_366 0.34 -0.0
434,811 + Pf1N1B4_366 0.43 -1.9
434,811 + Pf1N1B4_366 0.43 -0.0
434,811 + Pf1N1B4_366 0.43 -1.5
434,811 + Pf1N1B4_366 0.43 -0.0
434,812 - Pf1N1B4_366 0.43 -0.0
434,812 - Pf1N1B4_366 0.43 -0.0
434,812 - Pf1N1B4_366 0.43 -0.3
434,812 - Pf1N1B4_366 0.43 -0.0
434,812 - Pf1N1B4_366 0.43 -3.0
434,812 - Pf1N1B4_366 0.43 -0.0
434,886 + Pf1N1B4_366 0.57 -3.0
434,886 + -0.0
434,886 + Pf1N1B4_366 0.57 -0.0
434,886 + Pf1N1B4_366 0.57 -0.0
434,886 + Pf1N1B4_366 0.57 -0.0
434,886 + -0.0
434,887 - Pf1N1B4_366 0.57 -0.0
434,887 - Pf1N1B4_366 0.57 -0.0
434,887 - Pf1N1B4_366 0.57 -0.0
434,887 - Pf1N1B4_366 0.57 -0.0
434,887 - Pf1N1B4_366 0.57 -0.0
434,887 - Pf1N1B4_366 0.57 -2.2
434,887 - Pf1N1B4_366 0.57 -0.0
434,887 - Pf1N1B4_366 0.57 -0.0
434,967 + Pf1N1B4_366 0.73 -2.7
434,967 + Pf1N1B4_366 0.73 -2.6
434,967 + Pf1N1B4_366 0.73 -0.0
434,967 + Pf1N1B4_366 0.73 -0.0
434,967 + Pf1N1B4_366 0.73 -0.3
434,967 + Pf1N1B4_366 0.73 -0.0
434,967 + Pf1N1B4_366 0.73 -0.0
434,967 + Pf1N1B4_366 0.73 -0.0
434,967 + Pf1N1B4_366 0.73 -0.6
434,967 + Pf1N1B4_366 0.73 -0.0
434,967 + Pf1N1B4_366 0.73 -0.0
434,967 + Pf1N1B4_366 0.73 -3.3
434,968 - Pf1N1B4_366 0.73 -1.4
434,968 - Pf1N1B4_366 0.73 -0.0
434,968 - Pf1N1B4_366 0.73 -0.0
434,968 - Pf1N1B4_366 0.73 -0.0

Or see this region's nucleotide sequence