Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3204 and Pf1N1B4_3205 are separated by 95 nucleotides Pf1N1B4_3205 and Pf1N1B4_3206 are separated by 94 nucleotides Pf1N1B4_3206 and Pf1N1B4_3207 are separated by 105 nucleotides
Pf1N1B4_3204 - NAD(FAD)-utilizing dehydrogenase, sll0175 homolog, at 3,587,705 to 3,589,318
_3204
Pf1N1B4_3205 - Lipoprotein, putative, at 3,589,414 to 3,590,070
_3205
Pf1N1B4_3206 - Short chain dehydrogenase, at 3,590,165 to 3,590,764
_3206
Pf1N1B4_3207 - Transcriptional regulator, LysR family, at 3,590,870 to 3,591,778
_3207
Position (kb)
3590
3591 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3589.191 kb on + strand at 3589.192 kb on - strand at 3589.192 kb on - strand at 3589.314 kb on - strand at 3589.375 kb on + strand at 3589.375 kb on + strand at 3589.376 kb on - strand at 3589.376 kb on - strand at 3589.376 kb on - strand at 3589.377 kb on + strand at 3589.377 kb on + strand at 3589.377 kb on + strand at 3589.377 kb on + strand at 3589.378 kb on - strand at 3589.378 kb on - strand at 3589.378 kb on - strand at 3589.378 kb on - strand at 3589.439 kb on + strand at 3589.440 kb on - strand at 3589.611 kb on - strand, within Pf1N1B4_3205 at 3589.764 kb on - strand, within Pf1N1B4_3205 at 3589.927 kb on + strand, within Pf1N1B4_3205 at 3590.102 kb on - strand at 3590.195 kb on + strand at 3590.217 kb on + strand at 3590.265 kb on + strand, within Pf1N1B4_3206 at 3590.275 kb on - strand, within Pf1N1B4_3206 at 3590.275 kb on - strand, within Pf1N1B4_3206 at 3590.398 kb on + strand, within Pf1N1B4_3206 at 3590.772 kb on + strand at 3590.772 kb on + strand at 3590.772 kb on + strand at 3590.773 kb on - strand at 3590.773 kb on - strand at 3590.792 kb on - strand at 3590.792 kb on - strand at 3590.798 kb on - strand at 3590.821 kb on - strand at 3590.824 kb on + strand at 3590.824 kb on + strand at 3591.006 kb on + strand, within Pf1N1B4_3207 at 3591.063 kb on + strand, within Pf1N1B4_3207 at 3591.221 kb on + strand, within Pf1N1B4_3207 at 3591.256 kb on + strand, within Pf1N1B4_3207 at 3591.256 kb on + strand, within Pf1N1B4_3207 at 3591.257 kb on - strand, within Pf1N1B4_3207 at 3591.257 kb on - strand, within Pf1N1B4_3207 at 3591.257 kb on - strand, within Pf1N1B4_3207 at 3591.257 kb on - strand, within Pf1N1B4_3207 at 3591.257 kb on - strand, within Pf1N1B4_3207 at 3591.260 kb on + strand at 3591.260 kb on + strand, within Pf1N1B4_3207 at 3591.260 kb on + strand, within Pf1N1B4_3207 at 3591.260 kb on + strand, within Pf1N1B4_3207 at 3591.380 kb on + strand, within Pf1N1B4_3207 at 3591.380 kb on + strand at 3591.380 kb on + strand, within Pf1N1B4_3207 at 3591.381 kb on - strand, within Pf1N1B4_3207 at 3591.381 kb on - strand, within Pf1N1B4_3207 at 3591.381 kb on - strand, within Pf1N1B4_3207 at 3591.453 kb on - strand, within Pf1N1B4_3207 at 3591.476 kb on + strand, within Pf1N1B4_3207 at 3591.476 kb on + strand at 3591.477 kb on - strand, within Pf1N1B4_3207 at 3591.495 kb on + strand, within Pf1N1B4_3207 at 3591.495 kb on + strand, within Pf1N1B4_3207 at 3591.496 kb on - strand, within Pf1N1B4_3207 at 3591.536 kb on + strand at 3591.536 kb on + strand, within Pf1N1B4_3207 at 3591.536 kb on + strand, within Pf1N1B4_3207 at 3591.536 kb on + strand, within Pf1N1B4_3207 at 3591.537 kb on - strand, within Pf1N1B4_3207 at 3591.537 kb on - strand, within Pf1N1B4_3207 at 3591.593 kb on + strand, within Pf1N1B4_3207 at 3591.593 kb on + strand, within Pf1N1B4_3207 at 3591.594 kb on - strand at 3591.594 kb on - strand, within Pf1N1B4_3207 at 3591.671 kb on + strand, within Pf1N1B4_3207 at 3591.671 kb on + strand, within Pf1N1B4_3207 at 3591.671 kb on + strand, within Pf1N1B4_3207 at 3591.671 kb on + strand, within Pf1N1B4_3207 at 3591.672 kb on - strand, within Pf1N1B4_3207
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 3,589,191 + +0.0 3,589,192 - -3.8 3,589,192 - +0.0 3,589,314 - +0.0 3,589,375 + +0.0 3,589,375 + +0.0 3,589,376 - -0.7 3,589,376 - -1.0 3,589,376 - +0.0 3,589,377 + +0.0 3,589,377 + +0.0 3,589,377 + +0.0 3,589,377 + +0.0 3,589,378 - +0.0 3,589,378 - +0.0 3,589,378 - +0.0 3,589,378 - +0.0 3,589,439 + -2.7 3,589,440 - +0.0 3,589,611 - Pf1N1B4_3205 0.30 -0.0 3,589,764 - Pf1N1B4_3205 0.53 -0.0 3,589,927 + Pf1N1B4_3205 0.78 -0.0 3,590,102 - -0.0 3,590,195 + -0.0 3,590,217 + -0.0 3,590,265 + Pf1N1B4_3206 0.17 -0.0 3,590,275 - Pf1N1B4_3206 0.18 -2.4 3,590,275 - Pf1N1B4_3206 0.18 -0.0 3,590,398 + Pf1N1B4_3206 0.39 -2.8 3,590,772 + -0.0 3,590,772 + -0.0 3,590,772 + -0.0 3,590,773 - -0.0 3,590,773 - -0.0 3,590,792 - -0.0 3,590,792 - -0.0 3,590,798 - -2.0 3,590,821 - -0.0 3,590,824 + -1.8 3,590,824 + -0.0 3,591,006 + Pf1N1B4_3207 0.15 -0.0 3,591,063 + Pf1N1B4_3207 0.21 -0.0 3,591,221 + Pf1N1B4_3207 0.39 -0.0 3,591,256 + Pf1N1B4_3207 0.42 -0.0 3,591,256 + Pf1N1B4_3207 0.42 +1.0 3,591,257 - Pf1N1B4_3207 0.43 -0.0 3,591,257 - Pf1N1B4_3207 0.43 -0.0 3,591,257 - Pf1N1B4_3207 0.43 -1.6 3,591,257 - Pf1N1B4_3207 0.43 -2.1 3,591,257 - Pf1N1B4_3207 0.43 -0.0 3,591,260 + -0.0 3,591,260 + Pf1N1B4_3207 0.43 -0.0 3,591,260 + Pf1N1B4_3207 0.43 -1.5 3,591,260 + Pf1N1B4_3207 0.43 -0.0 3,591,380 + Pf1N1B4_3207 0.56 -0.0 3,591,380 + -0.0 3,591,380 + Pf1N1B4_3207 0.56 -0.0 3,591,381 - Pf1N1B4_3207 0.56 -1.9 3,591,381 - Pf1N1B4_3207 0.56 -0.0 3,591,381 - Pf1N1B4_3207 0.56 -1.8 3,591,453 - Pf1N1B4_3207 0.64 -0.0 3,591,476 + Pf1N1B4_3207 0.67 -0.0 3,591,476 + -2.1 3,591,477 - Pf1N1B4_3207 0.67 -0.0 3,591,495 + Pf1N1B4_3207 0.69 -0.0 3,591,495 + Pf1N1B4_3207 0.69 -0.0 3,591,496 - Pf1N1B4_3207 0.69 -1.3 3,591,536 + -0.0 3,591,536 + Pf1N1B4_3207 0.73 -0.0 3,591,536 + Pf1N1B4_3207 0.73 -0.0 3,591,536 + Pf1N1B4_3207 0.73 -0.0 3,591,537 - Pf1N1B4_3207 0.73 -0.0 3,591,537 - Pf1N1B4_3207 0.73 -2.5 3,591,593 + Pf1N1B4_3207 0.80 -1.9 3,591,593 + Pf1N1B4_3207 0.80 -0.0 3,591,594 - -1.9 3,591,594 - Pf1N1B4_3207 0.80 -1.1 3,591,671 + Pf1N1B4_3207 0.88 -0.0 3,591,671 + Pf1N1B4_3207 0.88 -0.0 3,591,671 + Pf1N1B4_3207 0.88 -2.7 3,591,671 + Pf1N1B4_3207 0.88 -2.2 3,591,672 - Pf1N1B4_3207 0.88 -0.0
Or see this region's nucleotide sequence