Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3206

Experiment: Nitrate reduction

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3204 and Pf1N1B4_3205 are separated by 95 nucleotidesPf1N1B4_3205 and Pf1N1B4_3206 are separated by 94 nucleotidesPf1N1B4_3206 and Pf1N1B4_3207 are separated by 105 nucleotides Pf1N1B4_3204 - NAD(FAD)-utilizing dehydrogenase, sll0175 homolog, at 3,587,705 to 3,589,318 _3204 Pf1N1B4_3205 - Lipoprotein, putative, at 3,589,414 to 3,590,070 _3205 Pf1N1B4_3206 - Short chain dehydrogenase, at 3,590,165 to 3,590,764 _3206 Pf1N1B4_3207 - Transcriptional regulator, LysR family, at 3,590,870 to 3,591,778 _3207 Position (kb) 3590 3591Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3589.191 kb on + strandat 3589.192 kb on - strandat 3589.192 kb on - strandat 3589.314 kb on - strandat 3589.375 kb on + strandat 3589.375 kb on + strandat 3589.376 kb on - strandat 3589.376 kb on - strandat 3589.376 kb on - strandat 3589.377 kb on + strandat 3589.377 kb on + strandat 3589.377 kb on + strandat 3589.377 kb on + strandat 3589.378 kb on - strandat 3589.378 kb on - strandat 3589.378 kb on - strandat 3589.378 kb on - strandat 3589.439 kb on + strandat 3589.440 kb on - strandat 3589.611 kb on - strand, within Pf1N1B4_3205at 3589.764 kb on - strand, within Pf1N1B4_3205at 3589.927 kb on + strand, within Pf1N1B4_3205at 3590.102 kb on - strandat 3590.195 kb on + strandat 3590.217 kb on + strandat 3590.265 kb on + strand, within Pf1N1B4_3206at 3590.275 kb on - strand, within Pf1N1B4_3206at 3590.275 kb on - strand, within Pf1N1B4_3206at 3590.398 kb on + strand, within Pf1N1B4_3206at 3590.772 kb on + strandat 3590.772 kb on + strandat 3590.772 kb on + strandat 3590.773 kb on - strandat 3590.773 kb on - strandat 3590.792 kb on - strandat 3590.792 kb on - strandat 3590.798 kb on - strandat 3590.821 kb on - strandat 3590.824 kb on + strandat 3590.824 kb on + strandat 3591.006 kb on + strand, within Pf1N1B4_3207at 3591.063 kb on + strand, within Pf1N1B4_3207at 3591.221 kb on + strand, within Pf1N1B4_3207at 3591.256 kb on + strand, within Pf1N1B4_3207at 3591.256 kb on + strand, within Pf1N1B4_3207at 3591.257 kb on - strand, within Pf1N1B4_3207at 3591.257 kb on - strand, within Pf1N1B4_3207at 3591.257 kb on - strand, within Pf1N1B4_3207at 3591.257 kb on - strand, within Pf1N1B4_3207at 3591.257 kb on - strand, within Pf1N1B4_3207at 3591.260 kb on + strandat 3591.260 kb on + strand, within Pf1N1B4_3207at 3591.260 kb on + strand, within Pf1N1B4_3207at 3591.260 kb on + strand, within Pf1N1B4_3207at 3591.380 kb on + strand, within Pf1N1B4_3207at 3591.380 kb on + strandat 3591.380 kb on + strand, within Pf1N1B4_3207at 3591.381 kb on - strand, within Pf1N1B4_3207at 3591.381 kb on - strand, within Pf1N1B4_3207at 3591.381 kb on - strand, within Pf1N1B4_3207at 3591.453 kb on - strand, within Pf1N1B4_3207at 3591.476 kb on + strand, within Pf1N1B4_3207at 3591.476 kb on + strandat 3591.477 kb on - strand, within Pf1N1B4_3207at 3591.495 kb on + strand, within Pf1N1B4_3207at 3591.495 kb on + strand, within Pf1N1B4_3207at 3591.496 kb on - strand, within Pf1N1B4_3207at 3591.536 kb on + strandat 3591.536 kb on + strand, within Pf1N1B4_3207at 3591.536 kb on + strand, within Pf1N1B4_3207at 3591.536 kb on + strand, within Pf1N1B4_3207at 3591.537 kb on - strand, within Pf1N1B4_3207at 3591.537 kb on - strand, within Pf1N1B4_3207at 3591.593 kb on + strand, within Pf1N1B4_3207at 3591.593 kb on + strand, within Pf1N1B4_3207at 3591.594 kb on - strandat 3591.594 kb on - strand, within Pf1N1B4_3207at 3591.671 kb on + strand, within Pf1N1B4_3207at 3591.671 kb on + strand, within Pf1N1B4_3207at 3591.671 kb on + strand, within Pf1N1B4_3207at 3591.671 kb on + strand, within Pf1N1B4_3207at 3591.672 kb on - strand, within Pf1N1B4_3207

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction
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3,589,191 + +0.0
3,589,192 - -3.8
3,589,192 - +0.0
3,589,314 - +0.0
3,589,375 + +0.0
3,589,375 + +0.0
3,589,376 - -0.7
3,589,376 - -1.0
3,589,376 - +0.0
3,589,377 + +0.0
3,589,377 + +0.0
3,589,377 + +0.0
3,589,377 + +0.0
3,589,378 - +0.0
3,589,378 - +0.0
3,589,378 - +0.0
3,589,378 - +0.0
3,589,439 + -2.7
3,589,440 - +0.0
3,589,611 - Pf1N1B4_3205 0.30 -0.0
3,589,764 - Pf1N1B4_3205 0.53 -0.0
3,589,927 + Pf1N1B4_3205 0.78 -0.0
3,590,102 - -0.0
3,590,195 + -0.0
3,590,217 + -0.0
3,590,265 + Pf1N1B4_3206 0.17 -0.0
3,590,275 - Pf1N1B4_3206 0.18 -2.4
3,590,275 - Pf1N1B4_3206 0.18 -0.0
3,590,398 + Pf1N1B4_3206 0.39 -2.8
3,590,772 + -0.0
3,590,772 + -0.0
3,590,772 + -0.0
3,590,773 - -0.0
3,590,773 - -0.0
3,590,792 - -0.0
3,590,792 - -0.0
3,590,798 - -2.0
3,590,821 - -0.0
3,590,824 + -1.8
3,590,824 + -0.0
3,591,006 + Pf1N1B4_3207 0.15 -0.0
3,591,063 + Pf1N1B4_3207 0.21 -0.0
3,591,221 + Pf1N1B4_3207 0.39 -0.0
3,591,256 + Pf1N1B4_3207 0.42 -0.0
3,591,256 + Pf1N1B4_3207 0.42 +1.0
3,591,257 - Pf1N1B4_3207 0.43 -0.0
3,591,257 - Pf1N1B4_3207 0.43 -0.0
3,591,257 - Pf1N1B4_3207 0.43 -1.6
3,591,257 - Pf1N1B4_3207 0.43 -2.1
3,591,257 - Pf1N1B4_3207 0.43 -0.0
3,591,260 + -0.0
3,591,260 + Pf1N1B4_3207 0.43 -0.0
3,591,260 + Pf1N1B4_3207 0.43 -1.5
3,591,260 + Pf1N1B4_3207 0.43 -0.0
3,591,380 + Pf1N1B4_3207 0.56 -0.0
3,591,380 + -0.0
3,591,380 + Pf1N1B4_3207 0.56 -0.0
3,591,381 - Pf1N1B4_3207 0.56 -1.9
3,591,381 - Pf1N1B4_3207 0.56 -0.0
3,591,381 - Pf1N1B4_3207 0.56 -1.8
3,591,453 - Pf1N1B4_3207 0.64 -0.0
3,591,476 + Pf1N1B4_3207 0.67 -0.0
3,591,476 + -2.1
3,591,477 - Pf1N1B4_3207 0.67 -0.0
3,591,495 + Pf1N1B4_3207 0.69 -0.0
3,591,495 + Pf1N1B4_3207 0.69 -0.0
3,591,496 - Pf1N1B4_3207 0.69 -1.3
3,591,536 + -0.0
3,591,536 + Pf1N1B4_3207 0.73 -0.0
3,591,536 + Pf1N1B4_3207 0.73 -0.0
3,591,536 + Pf1N1B4_3207 0.73 -0.0
3,591,537 - Pf1N1B4_3207 0.73 -0.0
3,591,537 - Pf1N1B4_3207 0.73 -2.5
3,591,593 + Pf1N1B4_3207 0.80 -1.9
3,591,593 + Pf1N1B4_3207 0.80 -0.0
3,591,594 - -1.9
3,591,594 - Pf1N1B4_3207 0.80 -1.1
3,591,671 + Pf1N1B4_3207 0.88 -0.0
3,591,671 + Pf1N1B4_3207 0.88 -0.0
3,591,671 + Pf1N1B4_3207 0.88 -2.7
3,591,671 + Pf1N1B4_3207 0.88 -2.2
3,591,672 - Pf1N1B4_3207 0.88 -0.0

Or see this region's nucleotide sequence