Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2986 and Pf1N1B4_2987 are separated by 150 nucleotides Pf1N1B4_2987 and Pf1N1B4_2988 overlap by 4 nucleotides
Pf1N1B4_2986 - sensor histidine kinase, at 3,353,226 to 3,354,611
_2986
Pf1N1B4_2987 - NAD-dependent formate dehydrogenase delta subunit, at 3,354,762 to 3,354,983
_2987
Pf1N1B4_2988 - NAD-dependent formate dehydrogenase alpha subunit, at 3,354,980 to 3,357,862
_2988
Position (kb)
3354
3355 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3353.941 kb on + strand, within Pf1N1B4_2986 at 3353.941 kb on + strand, within Pf1N1B4_2986 at 3353.941 kb on + strand, within Pf1N1B4_2986 at 3353.942 kb on - strand, within Pf1N1B4_2986 at 3353.942 kb on - strand, within Pf1N1B4_2986 at 3353.942 kb on - strand, within Pf1N1B4_2986 at 3354.192 kb on + strand, within Pf1N1B4_2986 at 3354.215 kb on + strand, within Pf1N1B4_2986 at 3354.215 kb on + strand, within Pf1N1B4_2986 at 3354.215 kb on + strand, within Pf1N1B4_2986 at 3354.215 kb on + strand, within Pf1N1B4_2986 at 3354.216 kb on - strand, within Pf1N1B4_2986 at 3354.216 kb on - strand, within Pf1N1B4_2986 at 3354.243 kb on + strand, within Pf1N1B4_2986 at 3354.243 kb on + strand, within Pf1N1B4_2986 at 3354.243 kb on + strand, within Pf1N1B4_2986 at 3354.244 kb on - strand, within Pf1N1B4_2986 at 3354.244 kb on - strand, within Pf1N1B4_2986 at 3354.266 kb on + strand, within Pf1N1B4_2986 at 3354.267 kb on - strand, within Pf1N1B4_2986 at 3354.278 kb on + strand, within Pf1N1B4_2986 at 3354.306 kb on + strand, within Pf1N1B4_2986 at 3354.306 kb on + strand, within Pf1N1B4_2986 at 3354.307 kb on - strand, within Pf1N1B4_2986 at 3354.364 kb on + strand, within Pf1N1B4_2986 at 3354.385 kb on + strand, within Pf1N1B4_2986 at 3354.386 kb on - strand, within Pf1N1B4_2986 at 3354.562 kb on + strand at 3354.563 kb on - strand at 3354.604 kb on + strand at 3354.604 kb on + strand at 3354.605 kb on - strand at 3354.605 kb on - strand at 3354.697 kb on + strand at 3354.697 kb on + strand at 3354.698 kb on - strand at 3354.698 kb on - strand at 3354.747 kb on + strand at 3354.747 kb on + strand at 3354.747 kb on + strand at 3354.747 kb on + strand at 3354.748 kb on - strand at 3354.748 kb on - strand at 3354.763 kb on + strand at 3354.763 kb on + strand at 3354.763 kb on + strand at 3354.763 kb on + strand at 3354.764 kb on - strand at 3354.764 kb on - strand at 3354.874 kb on + strand, within Pf1N1B4_2987 at 3354.874 kb on + strand, within Pf1N1B4_2987 at 3354.874 kb on + strand, within Pf1N1B4_2987 at 3354.874 kb on + strand at 3354.875 kb on - strand, within Pf1N1B4_2987 at 3354.875 kb on - strand, within Pf1N1B4_2987 at 3354.875 kb on - strand, within Pf1N1B4_2987 at 3354.875 kb on - strand, within Pf1N1B4_2987 at 3354.937 kb on + strand, within Pf1N1B4_2987 at 3354.937 kb on + strand, within Pf1N1B4_2987 at 3354.937 kb on + strand, within Pf1N1B4_2987 at 3354.938 kb on - strand, within Pf1N1B4_2987 at 3354.938 kb on - strand, within Pf1N1B4_2987 at 3354.990 kb on - strand at 3355.089 kb on + strand at 3355.089 kb on + strand at 3355.090 kb on - strand at 3355.122 kb on + strand at 3355.123 kb on - strand at 3355.123 kb on - strand at 3355.123 kb on - strand at 3355.203 kb on + strand at 3355.203 kb on + strand at 3355.203 kb on + strand at 3355.203 kb on + strand at 3355.203 kb on + strand at 3355.203 kb on + strand at 3355.203 kb on + strand at 3355.204 kb on - strand at 3355.204 kb on - strand at 3355.204 kb on - strand at 3355.204 kb on - strand at 3355.204 kb on - strand at 3355.204 kb on - strand at 3355.247 kb on + strand at 3355.392 kb on + strand, within Pf1N1B4_2988 at 3355.392 kb on + strand, within Pf1N1B4_2988 at 3355.392 kb on + strand, within Pf1N1B4_2988 at 3355.392 kb on + strand, within Pf1N1B4_2988 at 3355.393 kb on - strand, within Pf1N1B4_2988 at 3355.393 kb on - strand, within Pf1N1B4_2988 at 3355.587 kb on + strand, within Pf1N1B4_2988 at 3355.588 kb on - strand, within Pf1N1B4_2988 at 3355.653 kb on + strand, within Pf1N1B4_2988 at 3355.654 kb on - strand, within Pf1N1B4_2988 at 3355.654 kb on - strand, within Pf1N1B4_2988 at 3355.718 kb on + strand, within Pf1N1B4_2988 at 3355.948 kb on + strand, within Pf1N1B4_2988
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 3,353,941 + Pf1N1B4_2986 0.52 +0.0 3,353,941 + Pf1N1B4_2986 0.52 +0.0 3,353,941 + Pf1N1B4_2986 0.52 +0.0 3,353,942 - Pf1N1B4_2986 0.52 +0.0 3,353,942 - Pf1N1B4_2986 0.52 +0.0 3,353,942 - Pf1N1B4_2986 0.52 +0.0 3,354,192 + Pf1N1B4_2986 0.70 +0.0 3,354,215 + Pf1N1B4_2986 0.71 -1.3 3,354,215 + Pf1N1B4_2986 0.71 +0.0 3,354,215 + Pf1N1B4_2986 0.71 +0.0 3,354,215 + Pf1N1B4_2986 0.71 -2.0 3,354,216 - Pf1N1B4_2986 0.71 -1.3 3,354,216 - Pf1N1B4_2986 0.71 -3.6 3,354,243 + Pf1N1B4_2986 0.73 +0.0 3,354,243 + Pf1N1B4_2986 0.73 +0.0 3,354,243 + Pf1N1B4_2986 0.73 +0.0 3,354,244 - Pf1N1B4_2986 0.73 +0.0 3,354,244 - Pf1N1B4_2986 0.73 +0.0 3,354,266 + Pf1N1B4_2986 0.75 -1.9 3,354,267 - Pf1N1B4_2986 0.75 -2.4 3,354,278 + Pf1N1B4_2986 0.76 +0.0 3,354,306 + Pf1N1B4_2986 0.78 -2.7 3,354,306 + Pf1N1B4_2986 0.78 -1.3 3,354,307 - Pf1N1B4_2986 0.78 +0.0 3,354,364 + Pf1N1B4_2986 0.82 +0.0 3,354,385 + Pf1N1B4_2986 0.84 -2.8 3,354,386 - Pf1N1B4_2986 0.84 +0.0 3,354,562 + -4.0 3,354,563 - +0.0 3,354,604 + -1.0 3,354,604 + +0.0 3,354,605 - -4.0 3,354,605 - -1.3 3,354,697 + +0.0 3,354,697 + +0.0 3,354,698 - +0.0 3,354,698 - +0.0 3,354,747 + +0.0 3,354,747 + -2.1 3,354,747 + +0.0 3,354,747 + -2.9 3,354,748 - +0.0 3,354,748 - -2.1 3,354,763 + +0.0 3,354,763 + -1.5 3,354,763 + +0.0 3,354,763 + +0.0 3,354,764 - +0.0 3,354,764 - +0.0 3,354,874 + Pf1N1B4_2987 0.50 +0.0 3,354,874 + Pf1N1B4_2987 0.50 -1.7 3,354,874 + Pf1N1B4_2987 0.50 +0.0 3,354,874 + -1.7 3,354,875 - Pf1N1B4_2987 0.51 -3.7 3,354,875 - Pf1N1B4_2987 0.51 -1.0 3,354,875 - Pf1N1B4_2987 0.51 +0.0 3,354,875 - Pf1N1B4_2987 0.51 +0.0 3,354,937 + Pf1N1B4_2987 0.79 +0.0 3,354,937 + Pf1N1B4_2987 0.79 +0.0 3,354,937 + Pf1N1B4_2987 0.79 +0.0 3,354,938 - Pf1N1B4_2987 0.79 -1.5 3,354,938 - Pf1N1B4_2987 0.79 +0.0 3,354,990 - -2.4 3,355,089 + +0.0 3,355,089 + -1.0 3,355,090 - -1.7 3,355,122 + +0.0 3,355,123 - +0.0 3,355,123 - -5.7 3,355,123 - +0.0 3,355,203 + +0.0 3,355,203 + +0.0 3,355,203 + -1.0 3,355,203 + +0.0 3,355,203 + -2.9 3,355,203 + -1.1 3,355,203 + +0.0 3,355,204 - +0.0 3,355,204 - +0.0 3,355,204 - +0.0 3,355,204 - +0.0 3,355,204 - -2.0 3,355,204 - -1.5 3,355,247 + +0.0 3,355,392 + Pf1N1B4_2988 0.14 +0.0 3,355,392 + Pf1N1B4_2988 0.14 +0.0 3,355,392 + Pf1N1B4_2988 0.14 +0.0 3,355,392 + Pf1N1B4_2988 0.14 +0.0 3,355,393 - Pf1N1B4_2988 0.14 -1.9 3,355,393 - Pf1N1B4_2988 0.14 +0.0 3,355,587 + Pf1N1B4_2988 0.21 -1.0 3,355,588 - Pf1N1B4_2988 0.21 -2.2 3,355,653 + Pf1N1B4_2988 0.23 +0.0 3,355,654 - Pf1N1B4_2988 0.23 +0.3 3,355,654 - Pf1N1B4_2988 0.23 -3.2 3,355,718 + Pf1N1B4_2988 0.26 +0.0 3,355,948 + Pf1N1B4_2988 0.34 +0.0
Or see this region's nucleotide sequence