Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2870 and Pf1N1B4_2871 are separated by 231 nucleotides Pf1N1B4_2871 and Pf1N1B4_2872 are separated by 91 nucleotides Pf1N1B4_2872 and Pf1N1B4_2873 overlap by 4 nucleotides Pf1N1B4_2873 and Pf1N1B4_2874 are separated by 24 nucleotides
Pf1N1B4_2870 - Regulator of competence-specific genes, at 3,217,605 to 3,217,877
_2870
Pf1N1B4_2871 - FIG00954216: hypothetical protein, at 3,218,109 to 3,218,453
_2871
Pf1N1B4_2872 - FIG00955330: hypothetical protein, at 3,218,545 to 3,220,101
_2872
Pf1N1B4_2873 - hypothetical protein, at 3,220,098 to 3,220,244
_2873
Pf1N1B4_2874 - Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-), at 3,220,269 to 3,222,536
_2874
Position (kb)
3218
3219
3220
3221 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3217.561 kb on + strand at 3217.561 kb on + strand at 3217.561 kb on + strand at 3217.562 kb on - strand at 3217.562 kb on - strand at 3217.562 kb on - strand at 3217.562 kb on - strand at 3217.562 kb on - strand at 3217.562 kb on - strand at 3217.562 kb on - strand at 3217.588 kb on + strand at 3217.594 kb on + strand at 3217.594 kb on + strand at 3217.594 kb on + strand at 3217.595 kb on - strand at 3217.595 kb on - strand at 3217.595 kb on - strand at 3217.628 kb on + strand at 3217.629 kb on - strand at 3217.629 kb on - strand at 3217.629 kb on - strand at 3217.629 kb on - strand at 3217.630 kb on - strand at 3217.715 kb on - strand, within Pf1N1B4_2870 at 3217.715 kb on - strand, within Pf1N1B4_2870 at 3217.715 kb on - strand, within Pf1N1B4_2870 at 3217.929 kb on + strand at 3217.940 kb on - strand at 3217.984 kb on - strand at 3217.997 kb on + strand at 3217.998 kb on - strand at 3217.998 kb on - strand at 3218.052 kb on + strand at 3218.097 kb on - strand at 3218.188 kb on + strand, within Pf1N1B4_2871 at 3218.188 kb on + strand, within Pf1N1B4_2871 at 3218.189 kb on - strand, within Pf1N1B4_2871 at 3218.189 kb on - strand, within Pf1N1B4_2871 at 3218.278 kb on + strand, within Pf1N1B4_2871 at 3218.278 kb on + strand, within Pf1N1B4_2871 at 3218.279 kb on - strand, within Pf1N1B4_2871 at 3218.279 kb on - strand, within Pf1N1B4_2871 at 3218.279 kb on - strand, within Pf1N1B4_2871 at 3218.279 kb on - strand, within Pf1N1B4_2871 at 3218.279 kb on - strand, within Pf1N1B4_2871 at 3218.358 kb on - strand, within Pf1N1B4_2871 at 3218.358 kb on - strand, within Pf1N1B4_2871 at 3218.384 kb on + strand, within Pf1N1B4_2871 at 3218.419 kb on + strand at 3218.420 kb on - strand at 3218.538 kb on + strand at 3218.538 kb on + strand at 3218.546 kb on + strand at 3218.546 kb on + strand at 3218.709 kb on + strand, within Pf1N1B4_2872 at 3218.709 kb on + strand, within Pf1N1B4_2872 at 3218.709 kb on + strand, within Pf1N1B4_2872 at 3218.709 kb on + strand, within Pf1N1B4_2872 at 3218.710 kb on - strand, within Pf1N1B4_2872 at 3218.710 kb on - strand, within Pf1N1B4_2872 at 3218.710 kb on - strand, within Pf1N1B4_2872 at 3218.710 kb on - strand, within Pf1N1B4_2872 at 3218.710 kb on - strand, within Pf1N1B4_2872 at 3218.710 kb on - strand, within Pf1N1B4_2872 at 3218.710 kb on - strand, within Pf1N1B4_2872 at 3218.710 kb on - strand, within Pf1N1B4_2872 at 3218.840 kb on + strand, within Pf1N1B4_2872 at 3218.841 kb on - strand, within Pf1N1B4_2872 at 3218.841 kb on - strand, within Pf1N1B4_2872 at 3218.841 kb on - strand, within Pf1N1B4_2872 at 3218.841 kb on - strand, within Pf1N1B4_2872 at 3218.841 kb on - strand, within Pf1N1B4_2872 at 3218.841 kb on - strand, within Pf1N1B4_2872 at 3218.909 kb on + strand, within Pf1N1B4_2872 at 3218.909 kb on + strand, within Pf1N1B4_2872 at 3218.909 kb on + strand, within Pf1N1B4_2872 at 3218.910 kb on - strand, within Pf1N1B4_2872 at 3218.910 kb on - strand, within Pf1N1B4_2872 at 3218.910 kb on - strand, within Pf1N1B4_2872 at 3218.910 kb on - strand at 3218.935 kb on - strand, within Pf1N1B4_2872 at 3218.936 kb on + strand, within Pf1N1B4_2872 at 3218.936 kb on + strand, within Pf1N1B4_2872 at 3218.936 kb on + strand, within Pf1N1B4_2872 at 3218.936 kb on + strand, within Pf1N1B4_2872 at 3218.936 kb on + strand, within Pf1N1B4_2872 at 3218.936 kb on + strand at 3218.936 kb on + strand, within Pf1N1B4_2872 at 3218.936 kb on + strand, within Pf1N1B4_2872 at 3218.936 kb on + strand, within Pf1N1B4_2872 at 3218.937 kb on - strand, within Pf1N1B4_2872 at 3218.937 kb on - strand at 3218.937 kb on - strand, within Pf1N1B4_2872 at 3218.941 kb on - strand, within Pf1N1B4_2872 at 3218.941 kb on - strand, within Pf1N1B4_2872 at 3218.982 kb on + strand, within Pf1N1B4_2872 at 3218.982 kb on + strand, within Pf1N1B4_2872 at 3218.982 kb on + strand, within Pf1N1B4_2872 at 3218.982 kb on + strand, within Pf1N1B4_2872 at 3218.982 kb on + strand, within Pf1N1B4_2872 at 3218.982 kb on + strand, within Pf1N1B4_2872 at 3218.982 kb on + strand, within Pf1N1B4_2872 at 3218.982 kb on + strand, within Pf1N1B4_2872 at 3218.982 kb on + strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3219.113 kb on + strand, within Pf1N1B4_2872 at 3219.113 kb on + strand, within Pf1N1B4_2872 at 3219.113 kb on + strand, within Pf1N1B4_2872 at 3219.113 kb on + strand, within Pf1N1B4_2872 at 3219.113 kb on + strand, within Pf1N1B4_2872 at 3219.113 kb on + strand, within Pf1N1B4_2872 at 3219.114 kb on - strand, within Pf1N1B4_2872 at 3219.114 kb on - strand, within Pf1N1B4_2872 at 3219.114 kb on - strand, within Pf1N1B4_2872 at 3219.114 kb on - strand, within Pf1N1B4_2872 at 3219.114 kb on - strand, within Pf1N1B4_2872 at 3219.114 kb on - strand, within Pf1N1B4_2872 at 3219.114 kb on - strand, within Pf1N1B4_2872 at 3219.114 kb on - strand, within Pf1N1B4_2872 at 3219.114 kb on - strand, within Pf1N1B4_2872 at 3219.114 kb on - strand, within Pf1N1B4_2872 at 3219.134 kb on + strand, within Pf1N1B4_2872 at 3219.160 kb on - strand, within Pf1N1B4_2872 at 3219.239 kb on + strand, within Pf1N1B4_2872 at 3219.266 kb on + strand, within Pf1N1B4_2872 at 3219.492 kb on + strand, within Pf1N1B4_2872 at 3219.870 kb on - strand, within Pf1N1B4_2872 at 3219.870 kb on - strand, within Pf1N1B4_2872 at 3219.981 kb on + strand at 3220.206 kb on + strand, within Pf1N1B4_2873 at 3220.206 kb on + strand, within Pf1N1B4_2873 at 3220.206 kb on + strand, within Pf1N1B4_2873 at 3220.206 kb on + strand, within Pf1N1B4_2873 at 3220.206 kb on + strand, within Pf1N1B4_2873 at 3220.208 kb on + strand, within Pf1N1B4_2873 at 3220.209 kb on - strand, within Pf1N1B4_2873 at 3220.795 kb on - strand, within Pf1N1B4_2874 at 3220.840 kb on - strand, within Pf1N1B4_2874 at 3221.010 kb on + strand, within Pf1N1B4_2874
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 3,217,561 + -2.9 3,217,561 + +0.1 3,217,561 + +0.1 3,217,562 - +0.1 3,217,562 - +0.1 3,217,562 - +0.1 3,217,562 - +0.1 3,217,562 - +0.1 3,217,562 - +0.1 3,217,562 - -2.5 3,217,588 + -1.2 3,217,594 + +0.1 3,217,594 + +0.1 3,217,594 + +0.1 3,217,595 - -2.1 3,217,595 - +0.1 3,217,595 - +0.1 3,217,628 + +0.1 3,217,629 - -2.1 3,217,629 - +0.1 3,217,629 - +0.7 3,217,629 - -1.8 3,217,630 - -3.8 3,217,715 - Pf1N1B4_2870 0.40 +0.1 3,217,715 - Pf1N1B4_2870 0.40 +0.1 3,217,715 - Pf1N1B4_2870 0.40 +0.1 3,217,929 + +0.1 3,217,940 - -0.7 3,217,984 - +0.1 3,217,997 + +0.1 3,217,998 - -2.2 3,217,998 - +0.1 3,218,052 + -0.4 3,218,097 - -2.0 3,218,188 + Pf1N1B4_2871 0.23 +0.1 3,218,188 + Pf1N1B4_2871 0.23 +0.1 3,218,189 - Pf1N1B4_2871 0.23 +0.1 3,218,189 - Pf1N1B4_2871 0.23 +0.1 3,218,278 + Pf1N1B4_2871 0.49 -1.6 3,218,278 + Pf1N1B4_2871 0.49 +0.1 3,218,279 - Pf1N1B4_2871 0.49 +0.1 3,218,279 - Pf1N1B4_2871 0.49 +0.1 3,218,279 - Pf1N1B4_2871 0.49 +0.1 3,218,279 - Pf1N1B4_2871 0.49 +0.1 3,218,279 - Pf1N1B4_2871 0.49 +0.1 3,218,358 - Pf1N1B4_2871 0.72 +0.1 3,218,358 - Pf1N1B4_2871 0.72 +0.1 3,218,384 + Pf1N1B4_2871 0.80 +0.1 3,218,419 + +0.1 3,218,420 - +0.1 3,218,538 + -1.9 3,218,538 + +0.1 3,218,546 + +0.1 3,218,546 + +0.1 3,218,709 + Pf1N1B4_2872 0.11 +0.1 3,218,709 + Pf1N1B4_2872 0.11 -4.5 3,218,709 + Pf1N1B4_2872 0.11 -3.9 3,218,709 + Pf1N1B4_2872 0.11 +0.1 3,218,710 - Pf1N1B4_2872 0.11 -2.4 3,218,710 - Pf1N1B4_2872 0.11 +0.1 3,218,710 - Pf1N1B4_2872 0.11 +0.1 3,218,710 - Pf1N1B4_2872 0.11 -3.8 3,218,710 - Pf1N1B4_2872 0.11 -5.0 3,218,710 - Pf1N1B4_2872 0.11 +0.1 3,218,710 - Pf1N1B4_2872 0.11 -3.3 3,218,710 - Pf1N1B4_2872 0.11 -2.9 3,218,840 + Pf1N1B4_2872 0.19 +0.1 3,218,841 - Pf1N1B4_2872 0.19 +0.1 3,218,841 - Pf1N1B4_2872 0.19 +0.1 3,218,841 - Pf1N1B4_2872 0.19 -1.9 3,218,841 - Pf1N1B4_2872 0.19 +0.1 3,218,841 - Pf1N1B4_2872 0.19 +0.1 3,218,841 - Pf1N1B4_2872 0.19 -2.9 3,218,909 + Pf1N1B4_2872 0.23 +0.1 3,218,909 + Pf1N1B4_2872 0.23 +0.1 3,218,909 + Pf1N1B4_2872 0.23 +0.1 3,218,910 - Pf1N1B4_2872 0.23 -4.0 3,218,910 - Pf1N1B4_2872 0.23 +0.1 3,218,910 - Pf1N1B4_2872 0.23 -5.6 3,218,910 - +0.1 3,218,935 - Pf1N1B4_2872 0.25 -3.5 3,218,936 + Pf1N1B4_2872 0.25 -2.3 3,218,936 + Pf1N1B4_2872 0.25 -1.2 3,218,936 + Pf1N1B4_2872 0.25 -4.3 3,218,936 + Pf1N1B4_2872 0.25 +0.1 3,218,936 + Pf1N1B4_2872 0.25 +0.1 3,218,936 + -1.8 3,218,936 + Pf1N1B4_2872 0.25 -1.9 3,218,936 + Pf1N1B4_2872 0.25 +0.1 3,218,936 + Pf1N1B4_2872 0.25 +0.1 3,218,937 - Pf1N1B4_2872 0.25 +0.1 3,218,937 - +0.1 3,218,937 - Pf1N1B4_2872 0.25 -4.5 3,218,941 - Pf1N1B4_2872 0.25 +0.1 3,218,941 - Pf1N1B4_2872 0.25 +0.1 3,218,982 + Pf1N1B4_2872 0.28 +0.1 3,218,982 + Pf1N1B4_2872 0.28 +0.1 3,218,982 + Pf1N1B4_2872 0.28 +0.1 3,218,982 + Pf1N1B4_2872 0.28 +0.1 3,218,982 + Pf1N1B4_2872 0.28 -3.9 3,218,982 + Pf1N1B4_2872 0.28 +0.1 3,218,982 + Pf1N1B4_2872 0.28 +0.1 3,218,982 + Pf1N1B4_2872 0.28 -4.8 3,218,982 + Pf1N1B4_2872 0.28 -2.2 3,218,983 - Pf1N1B4_2872 0.28 +0.1 3,218,983 - Pf1N1B4_2872 0.28 +0.1 3,218,983 - Pf1N1B4_2872 0.28 +0.1 3,218,983 - Pf1N1B4_2872 0.28 -5.4 3,218,983 - Pf1N1B4_2872 0.28 -2.7 3,218,983 - Pf1N1B4_2872 0.28 -5.4 3,218,983 - Pf1N1B4_2872 0.28 +0.1 3,218,983 - Pf1N1B4_2872 0.28 -2.6 3,218,983 - Pf1N1B4_2872 0.28 +0.1 3,218,983 - Pf1N1B4_2872 0.28 -3.2 3,218,983 - Pf1N1B4_2872 0.28 -3.6 3,218,983 - Pf1N1B4_2872 0.28 +0.1 3,218,983 - Pf1N1B4_2872 0.28 +0.1 3,218,983 - Pf1N1B4_2872 0.28 +0.1 3,218,983 - Pf1N1B4_2872 0.28 +0.1 3,219,113 + Pf1N1B4_2872 0.36 +0.1 3,219,113 + Pf1N1B4_2872 0.36 -3.0 3,219,113 + Pf1N1B4_2872 0.36 +0.1 3,219,113 + Pf1N1B4_2872 0.36 -1.5 3,219,113 + Pf1N1B4_2872 0.36 -3.9 3,219,113 + Pf1N1B4_2872 0.36 -5.0 3,219,114 - Pf1N1B4_2872 0.37 -4.5 3,219,114 - Pf1N1B4_2872 0.37 +0.1 3,219,114 - Pf1N1B4_2872 0.37 +0.1 3,219,114 - Pf1N1B4_2872 0.37 +0.1 3,219,114 - Pf1N1B4_2872 0.37 +0.1 3,219,114 - Pf1N1B4_2872 0.37 -4.9 3,219,114 - Pf1N1B4_2872 0.37 -3.1 3,219,114 - Pf1N1B4_2872 0.37 +0.1 3,219,114 - Pf1N1B4_2872 0.37 +0.1 3,219,114 - Pf1N1B4_2872 0.37 +0.1 3,219,134 + Pf1N1B4_2872 0.38 -3.3 3,219,160 - Pf1N1B4_2872 0.39 +0.1 3,219,239 + Pf1N1B4_2872 0.45 +0.1 3,219,266 + Pf1N1B4_2872 0.46 -0.3 3,219,492 + Pf1N1B4_2872 0.61 -1.5 3,219,870 - Pf1N1B4_2872 0.85 +0.1 3,219,870 - Pf1N1B4_2872 0.85 +0.1 3,219,981 + -3.4 3,220,206 + Pf1N1B4_2873 0.73 -2.7 3,220,206 + Pf1N1B4_2873 0.73 -2.8 3,220,206 + Pf1N1B4_2873 0.73 -1.5 3,220,206 + Pf1N1B4_2873 0.73 -4.9 3,220,206 + Pf1N1B4_2873 0.73 +0.1 3,220,208 + Pf1N1B4_2873 0.75 +0.1 3,220,209 - Pf1N1B4_2873 0.76 -1.5 3,220,795 - Pf1N1B4_2874 0.23 -2.8 3,220,840 - Pf1N1B4_2874 0.25 +0.1 3,221,010 + Pf1N1B4_2874 0.33 +0.1
Or see this region's nucleotide sequence