Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2771 and Pf1N1B4_2772 are separated by 14 nucleotides Pf1N1B4_2772 and Pf1N1B4_2773 are separated by 161 nucleotides Pf1N1B4_2773 and Pf1N1B4_2774 are separated by 305 nucleotides Pf1N1B4_2774 and Pf1N1B4_2775 are separated by 25 nucleotides
Pf1N1B4_2771 - Carbon starvation protein A, at 3,109,227 to 3,111,293
_2771
Pf1N1B4_2772 - COG2879, Hypothetical small protein yjiX, at 3,111,308 to 3,111,505
_2772
Pf1N1B4_2773 - COG0523: Putative GTPases (G3E family), at 3,111,667 to 3,112,638
_2773
Pf1N1B4_2774 - hypothetical protein, at 3,112,944 to 3,113,093
_2774
Pf1N1B4_2775 - Lanthionine biosynthesis protein LanM, at 3,113,119 to 3,116,289
_2775
Position (kb)
3111
3112
3113 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3110.952 kb on + strand, within Pf1N1B4_2771 at 3110.953 kb on - strand, within Pf1N1B4_2771 at 3110.953 kb on - strand, within Pf1N1B4_2771 at 3110.953 kb on - strand, within Pf1N1B4_2771 at 3111.136 kb on + strand at 3111.136 kb on + strand at 3111.136 kb on + strand at 3111.136 kb on + strand at 3111.136 kb on + strand at 3111.136 kb on + strand at 3111.136 kb on + strand at 3111.136 kb on + strand at 3111.136 kb on + strand at 3111.136 kb on + strand at 3111.136 kb on + strand at 3111.136 kb on + strand at 3111.136 kb on + strand at 3111.137 kb on - strand at 3111.137 kb on - strand at 3111.137 kb on - strand at 3111.137 kb on - strand at 3111.137 kb on - strand at 3111.137 kb on - strand at 3111.137 kb on - strand at 3111.137 kb on - strand at 3111.137 kb on - strand at 3111.137 kb on - strand at 3111.137 kb on - strand at 3111.137 kb on - strand at 3111.137 kb on - strand at 3111.141 kb on + strand at 3111.141 kb on + strand at 3111.141 kb on + strand at 3111.141 kb on + strand at 3111.141 kb on + strand at 3111.141 kb on + strand at 3111.141 kb on + strand at 3111.141 kb on + strand at 3111.141 kb on + strand at 3111.141 kb on + strand at 3111.141 kb on + strand at 3111.141 kb on + strand at 3111.142 kb on - strand at 3111.142 kb on - strand at 3111.142 kb on - strand at 3111.142 kb on - strand at 3111.142 kb on - strand at 3111.142 kb on - strand at 3111.142 kb on - strand at 3111.142 kb on - strand at 3111.142 kb on - strand at 3111.142 kb on - strand at 3111.142 kb on - strand at 3111.151 kb on - strand at 3111.151 kb on - strand at 3111.151 kb on - strand at 3111.239 kb on + strand at 3111.335 kb on - strand, within Pf1N1B4_2772 at 3111.335 kb on - strand, within Pf1N1B4_2772 at 3111.338 kb on + strand, within Pf1N1B4_2772 at 3111.338 kb on + strand, within Pf1N1B4_2772 at 3111.380 kb on + strand, within Pf1N1B4_2772 at 3111.380 kb on + strand, within Pf1N1B4_2772 at 3111.389 kb on + strand, within Pf1N1B4_2772 at 3111.389 kb on + strand, within Pf1N1B4_2772 at 3111.389 kb on + strand, within Pf1N1B4_2772 at 3111.389 kb on + strand at 3111.390 kb on - strand, within Pf1N1B4_2772 at 3111.390 kb on - strand, within Pf1N1B4_2772 at 3111.390 kb on - strand, within Pf1N1B4_2772 at 3111.390 kb on - strand, within Pf1N1B4_2772 at 3111.390 kb on - strand, within Pf1N1B4_2772 at 3111.390 kb on - strand, within Pf1N1B4_2772 at 3111.390 kb on - strand, within Pf1N1B4_2772 at 3111.390 kb on - strand, within Pf1N1B4_2772 at 3111.390 kb on - strand, within Pf1N1B4_2772 at 3111.400 kb on + strand, within Pf1N1B4_2772 at 3111.400 kb on + strand, within Pf1N1B4_2772 at 3111.400 kb on + strand, within Pf1N1B4_2772 at 3111.400 kb on + strand, within Pf1N1B4_2772 at 3111.400 kb on + strand, within Pf1N1B4_2772 at 3111.400 kb on + strand, within Pf1N1B4_2772 at 3111.400 kb on + strand, within Pf1N1B4_2772 at 3111.401 kb on - strand, within Pf1N1B4_2772 at 3111.401 kb on - strand, within Pf1N1B4_2772 at 3111.401 kb on - strand at 3111.437 kb on + strand, within Pf1N1B4_2772 at 3111.641 kb on + strand at 3111.642 kb on - strand at 3111.642 kb on - strand at 3111.687 kb on - strand at 3111.901 kb on + strand, within Pf1N1B4_2773 at 3111.902 kb on - strand, within Pf1N1B4_2773 at 3111.902 kb on - strand, within Pf1N1B4_2773 at 3111.902 kb on - strand, within Pf1N1B4_2773 at 3112.030 kb on + strand, within Pf1N1B4_2773 at 3112.030 kb on + strand, within Pf1N1B4_2773 at 3112.031 kb on - strand, within Pf1N1B4_2773 at 3112.049 kb on + strand, within Pf1N1B4_2773 at 3112.049 kb on + strand, within Pf1N1B4_2773 at 3112.049 kb on + strand, within Pf1N1B4_2773 at 3112.049 kb on + strand, within Pf1N1B4_2773 at 3112.050 kb on - strand, within Pf1N1B4_2773 at 3112.050 kb on - strand, within Pf1N1B4_2773 at 3112.447 kb on + strand, within Pf1N1B4_2773 at 3112.448 kb on - strand, within Pf1N1B4_2773 at 3112.748 kb on - strand at 3112.748 kb on - strand at 3112.748 kb on - strand at 3112.891 kb on + strand at 3112.920 kb on - strand at 3112.998 kb on + strand, within Pf1N1B4_2774 at 3113.002 kb on + strand, within Pf1N1B4_2774 at 3113.002 kb on + strand, within Pf1N1B4_2774 at 3113.053 kb on + strand, within Pf1N1B4_2774 at 3113.053 kb on + strand, within Pf1N1B4_2774 at 3113.054 kb on - strand, within Pf1N1B4_2774 at 3113.054 kb on - strand at 3113.107 kb on + strand at 3113.107 kb on + strand at 3113.107 kb on + strand at 3113.108 kb on - strand at 3113.139 kb on + strand at 3113.139 kb on + strand at 3113.139 kb on + strand at 3113.139 kb on + strand at 3113.139 kb on + strand at 3113.140 kb on - strand at 3113.140 kb on - strand at 3113.140 kb on - strand at 3113.140 kb on - strand at 3113.140 kb on - strand at 3113.166 kb on - strand at 3113.211 kb on - strand at 3113.211 kb on - strand at 3113.229 kb on + strand at 3113.229 kb on + strand at 3113.271 kb on - strand at 3113.323 kb on - strand at 3113.391 kb on - strand at 3113.453 kb on - strand, within Pf1N1B4_2775 at 3113.506 kb on - strand, within Pf1N1B4_2775 at 3113.561 kb on - strand, within Pf1N1B4_2775
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 3,110,952 + Pf1N1B4_2771 0.83 -1.7 3,110,953 - Pf1N1B4_2771 0.84 +0.0 3,110,953 - Pf1N1B4_2771 0.84 -1.8 3,110,953 - Pf1N1B4_2771 0.84 -2.9 3,111,136 + -1.3 3,111,136 + +0.0 3,111,136 + +0.0 3,111,136 + +0.0 3,111,136 + +0.0 3,111,136 + +0.0 3,111,136 + -2.1 3,111,136 + -2.8 3,111,136 + -3.1 3,111,136 + +0.0 3,111,136 + -2.8 3,111,136 + +0.0 3,111,136 + +0.0 3,111,137 - +0.0 3,111,137 - -2.8 3,111,137 - -5.1 3,111,137 - +0.0 3,111,137 - +0.0 3,111,137 - +0.0 3,111,137 - +0.0 3,111,137 - +0.0 3,111,137 - +0.0 3,111,137 - +0.0 3,111,137 - +0.0 3,111,137 - +0.0 3,111,137 - +0.0 3,111,141 + +0.0 3,111,141 + +0.0 3,111,141 + -2.3 3,111,141 + +0.0 3,111,141 + +0.0 3,111,141 + -2.3 3,111,141 + +0.0 3,111,141 + +0.0 3,111,141 + +0.0 3,111,141 + +0.0 3,111,141 + -2.4 3,111,141 + +0.0 3,111,142 - +0.0 3,111,142 - -2.1 3,111,142 - -2.9 3,111,142 - +0.0 3,111,142 - -1.9 3,111,142 - +0.0 3,111,142 - -1.5 3,111,142 - -2.9 3,111,142 - +0.0 3,111,142 - +0.0 3,111,142 - +0.0 3,111,151 - +0.0 3,111,151 - +0.0 3,111,151 - +0.0 3,111,239 + +0.0 3,111,335 - Pf1N1B4_2772 0.14 -4.0 3,111,335 - Pf1N1B4_2772 0.14 +0.0 3,111,338 + Pf1N1B4_2772 0.15 +0.0 3,111,338 + Pf1N1B4_2772 0.15 -3.9 3,111,380 + Pf1N1B4_2772 0.36 -1.9 3,111,380 + Pf1N1B4_2772 0.36 +0.0 3,111,389 + Pf1N1B4_2772 0.41 -0.6 3,111,389 + Pf1N1B4_2772 0.41 -0.3 3,111,389 + Pf1N1B4_2772 0.41 +0.0 3,111,389 + +0.0 3,111,390 - Pf1N1B4_2772 0.41 -3.2 3,111,390 - Pf1N1B4_2772 0.41 +0.0 3,111,390 - Pf1N1B4_2772 0.41 +0.0 3,111,390 - Pf1N1B4_2772 0.41 +0.0 3,111,390 - Pf1N1B4_2772 0.41 +0.0 3,111,390 - Pf1N1B4_2772 0.41 +0.0 3,111,390 - Pf1N1B4_2772 0.41 +0.0 3,111,390 - Pf1N1B4_2772 0.41 -3.1 3,111,390 - Pf1N1B4_2772 0.41 -2.8 3,111,400 + Pf1N1B4_2772 0.46 +0.0 3,111,400 + Pf1N1B4_2772 0.46 +0.0 3,111,400 + Pf1N1B4_2772 0.46 -1.3 3,111,400 + Pf1N1B4_2772 0.46 +0.0 3,111,400 + Pf1N1B4_2772 0.46 +0.0 3,111,400 + Pf1N1B4_2772 0.46 -0.3 3,111,400 + Pf1N1B4_2772 0.46 +0.0 3,111,401 - Pf1N1B4_2772 0.47 +0.0 3,111,401 - Pf1N1B4_2772 0.47 +0.0 3,111,401 - +0.0 3,111,437 + Pf1N1B4_2772 0.65 +0.0 3,111,641 + -1.2 3,111,642 - -3.3 3,111,642 - +0.0 3,111,687 - -3.4 3,111,901 + Pf1N1B4_2773 0.24 +0.0 3,111,902 - Pf1N1B4_2773 0.24 +0.0 3,111,902 - Pf1N1B4_2773 0.24 +0.0 3,111,902 - Pf1N1B4_2773 0.24 +0.0 3,112,030 + Pf1N1B4_2773 0.37 +0.0 3,112,030 + Pf1N1B4_2773 0.37 +0.0 3,112,031 - Pf1N1B4_2773 0.37 +0.0 3,112,049 + Pf1N1B4_2773 0.39 -3.3 3,112,049 + Pf1N1B4_2773 0.39 +0.0 3,112,049 + Pf1N1B4_2773 0.39 -5.4 3,112,049 + Pf1N1B4_2773 0.39 -3.7 3,112,050 - Pf1N1B4_2773 0.39 +0.0 3,112,050 - Pf1N1B4_2773 0.39 -2.0 3,112,447 + Pf1N1B4_2773 0.80 -2.8 3,112,448 - Pf1N1B4_2773 0.80 +0.0 3,112,748 - +0.0 3,112,748 - +0.0 3,112,748 - +0.0 3,112,891 + -4.2 3,112,920 - -3.9 3,112,998 + Pf1N1B4_2774 0.36 -1.9 3,113,002 + Pf1N1B4_2774 0.39 +0.0 3,113,002 + Pf1N1B4_2774 0.39 -2.0 3,113,053 + Pf1N1B4_2774 0.73 +0.0 3,113,053 + Pf1N1B4_2774 0.73 +0.0 3,113,054 - Pf1N1B4_2774 0.73 +0.0 3,113,054 - -1.0 3,113,107 + -1.3 3,113,107 + -1.1 3,113,107 + +0.0 3,113,108 - -1.7 3,113,139 + +0.0 3,113,139 + +0.0 3,113,139 + -1.9 3,113,139 + +0.0 3,113,139 + -1.5 3,113,140 - -1.4 3,113,140 - +0.0 3,113,140 - +0.0 3,113,140 - +0.0 3,113,140 - +0.0 3,113,166 - -2.5 3,113,211 - +1.4 3,113,211 - +0.0 3,113,229 + +0.0 3,113,229 + -0.7 3,113,271 - -0.7 3,113,323 - -1.5 3,113,391 - -1.9 3,113,453 - Pf1N1B4_2775 0.11 +0.0 3,113,506 - Pf1N1B4_2775 0.12 -2.1 3,113,561 - Pf1N1B4_2775 0.14 -3.8
Or see this region's nucleotide sequence