Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2619 and Pf1N1B4_2620 are separated by 69 nucleotides Pf1N1B4_2620 and Pf1N1B4_2621 are separated by 156 nucleotides Pf1N1B4_2621 and Pf1N1B4_2622 are separated by 24 nucleotides Pf1N1B4_2622 and Pf1N1B4_2623 are separated by 322 nucleotides
Pf1N1B4_2619 - Putative potassium channel protein, at 2,959,392 to 2,960,426
_2619
Pf1N1B4_2620 - GlpG protein (membrane protein of glp regulon), at 2,960,496 to 2,961,059
_2620
Pf1N1B4_2621 - PspA/IM30 family protein, at 2,961,216 to 2,961,914
_2621
Pf1N1B4_2622 - FIG006163: hypothetical protein, at 2,961,939 to 2,962,520
_2622
Pf1N1B4_2623 - Isochorismatase (EC 3.3.2.1), at 2,962,843 to 2,963,391
_2623
Position (kb)
2961
2962 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2960.260 kb on + strand, within Pf1N1B4_2619 at 2960.260 kb on + strand, within Pf1N1B4_2619 at 2960.261 kb on - strand at 2960.261 kb on - strand, within Pf1N1B4_2619 at 2960.261 kb on - strand, within Pf1N1B4_2619 at 2960.261 kb on - strand, within Pf1N1B4_2619 at 2960.261 kb on - strand, within Pf1N1B4_2619 at 2960.266 kb on + strand, within Pf1N1B4_2619 at 2960.266 kb on + strand, within Pf1N1B4_2619 at 2960.266 kb on + strand, within Pf1N1B4_2619 at 2960.266 kb on + strand, within Pf1N1B4_2619 at 2960.267 kb on - strand, within Pf1N1B4_2619 at 2960.267 kb on - strand, within Pf1N1B4_2619 at 2960.267 kb on - strand, within Pf1N1B4_2619 at 2960.267 kb on - strand, within Pf1N1B4_2619 at 2960.267 kb on - strand, within Pf1N1B4_2619 at 2960.267 kb on - strand, within Pf1N1B4_2619 at 2960.270 kb on + strand, within Pf1N1B4_2619 at 2960.271 kb on - strand, within Pf1N1B4_2619 at 2960.323 kb on + strand at 2960.323 kb on + strand at 2960.323 kb on + strand at 2960.324 kb on - strand at 2960.324 kb on - strand at 2960.324 kb on - strand at 2960.324 kb on - strand at 2960.334 kb on + strand at 2960.334 kb on + strand at 2960.334 kb on + strand at 2960.334 kb on + strand at 2960.334 kb on + strand at 2960.334 kb on + strand at 2960.334 kb on + strand at 2960.334 kb on + strand at 2960.334 kb on + strand at 2960.334 kb on + strand at 2960.334 kb on + strand at 2960.335 kb on - strand at 2960.335 kb on - strand at 2960.335 kb on - strand at 2960.335 kb on - strand at 2960.335 kb on - strand at 2960.335 kb on - strand at 2960.335 kb on - strand at 2960.340 kb on + strand at 2960.340 kb on + strand at 2960.340 kb on + strand at 2960.340 kb on + strand at 2960.340 kb on + strand at 2960.341 kb on - strand at 2960.341 kb on - strand at 2960.341 kb on - strand at 2960.341 kb on - strand at 2960.398 kb on + strand at 2960.398 kb on + strand at 2960.398 kb on + strand at 2960.398 kb on + strand at 2960.399 kb on - strand at 2960.399 kb on - strand at 2960.399 kb on - strand at 2960.399 kb on - strand at 2960.409 kb on + strand at 2960.409 kb on + strand at 2960.409 kb on + strand at 2960.409 kb on + strand at 2960.410 kb on - strand at 2960.427 kb on + strand at 2960.427 kb on + strand at 2960.427 kb on + strand at 2960.427 kb on + strand at 2960.427 kb on + strand at 2960.427 kb on + strand at 2960.427 kb on + strand at 2960.427 kb on + strand at 2960.427 kb on + strand at 2960.427 kb on + strand at 2960.428 kb on - strand at 2960.428 kb on - strand at 2960.428 kb on - strand at 2960.463 kb on + strand at 2960.510 kb on + strand at 2960.510 kb on + strand at 2960.528 kb on + strand at 2960.532 kb on + strand at 2960.533 kb on - strand at 2960.538 kb on + strand at 2960.552 kb on + strand at 2960.552 kb on + strand at 2960.576 kb on + strand, within Pf1N1B4_2620 at 2960.576 kb on + strand, within Pf1N1B4_2620 at 2960.576 kb on + strand, within Pf1N1B4_2620 at 2960.576 kb on + strand, within Pf1N1B4_2620 at 2960.576 kb on + strand at 2960.576 kb on + strand, within Pf1N1B4_2620 at 2960.576 kb on + strand, within Pf1N1B4_2620 at 2960.577 kb on - strand, within Pf1N1B4_2620 at 2960.577 kb on - strand, within Pf1N1B4_2620 at 2960.577 kb on - strand, within Pf1N1B4_2620 at 2960.577 kb on - strand, within Pf1N1B4_2620 at 2960.632 kb on + strand, within Pf1N1B4_2620 at 2960.632 kb on + strand, within Pf1N1B4_2620 at 2960.633 kb on - strand, within Pf1N1B4_2620 at 2960.633 kb on - strand, within Pf1N1B4_2620 at 2960.705 kb on - strand, within Pf1N1B4_2620 at 2960.705 kb on - strand, within Pf1N1B4_2620 at 2960.792 kb on + strand, within Pf1N1B4_2620 at 2960.793 kb on - strand, within Pf1N1B4_2620 at 2960.798 kb on + strand, within Pf1N1B4_2620 at 2960.799 kb on - strand, within Pf1N1B4_2620 at 2960.846 kb on + strand, within Pf1N1B4_2620 at 2960.966 kb on + strand, within Pf1N1B4_2620 at 2960.967 kb on - strand, within Pf1N1B4_2620 at 2960.975 kb on - strand, within Pf1N1B4_2620 at 2961.084 kb on + strand at 2961.469 kb on - strand, within Pf1N1B4_2621 at 2961.712 kb on + strand, within Pf1N1B4_2621 at 2961.713 kb on - strand, within Pf1N1B4_2621 at 2961.920 kb on - strand at 2961.941 kb on - strand at 2962.058 kb on - strand, within Pf1N1B4_2622 at 2962.058 kb on - strand, within Pf1N1B4_2622 at 2962.058 kb on - strand, within Pf1N1B4_2622 at 2962.150 kb on - strand, within Pf1N1B4_2622 at 2962.220 kb on - strand, within Pf1N1B4_2622 at 2962.220 kb on - strand, within Pf1N1B4_2622 at 2962.220 kb on - strand, within Pf1N1B4_2622 at 2962.220 kb on - strand, within Pf1N1B4_2622 at 2962.336 kb on + strand, within Pf1N1B4_2622 at 2962.337 kb on - strand, within Pf1N1B4_2622 at 2962.337 kb on - strand, within Pf1N1B4_2622 at 2962.337 kb on - strand, within Pf1N1B4_2622 at 2962.359 kb on - strand, within Pf1N1B4_2622 at 2962.359 kb on - strand, within Pf1N1B4_2622 at 2962.362 kb on - strand, within Pf1N1B4_2622 at 2962.362 kb on - strand, within Pf1N1B4_2622 at 2962.375 kb on + strand, within Pf1N1B4_2622 at 2962.376 kb on - strand, within Pf1N1B4_2622 at 2962.376 kb on - strand, within Pf1N1B4_2622 at 2962.483 kb on + strand at 2962.484 kb on - strand at 2962.484 kb on - strand at 2962.484 kb on - strand at 2962.520 kb on + strand at 2962.520 kb on + strand at 2962.520 kb on + strand at 2962.521 kb on - strand at 2962.522 kb on + strand at 2962.522 kb on + strand at 2962.522 kb on + strand at 2962.522 kb on + strand at 2962.522 kb on + strand at 2962.522 kb on + strand at 2962.522 kb on + strand at 2962.523 kb on - strand at 2962.523 kb on - strand at 2962.529 kb on + strand at 2962.529 kb on + strand at 2962.530 kb on - strand at 2962.530 kb on - strand at 2962.530 kb on - strand at 2962.530 kb on - strand at 2962.629 kb on - strand at 2962.629 kb on - strand at 2962.670 kb on + strand at 2962.695 kb on + strand at 2962.695 kb on + strand at 2962.774 kb on + strand at 2962.774 kb on + strand at 2962.774 kb on + strand at 2962.775 kb on - strand at 2962.793 kb on + strand at 2962.793 kb on + strand at 2962.844 kb on + strand at 2962.844 kb on + strand at 2962.844 kb on + strand at 2962.844 kb on + strand at 2962.845 kb on - strand at 2962.845 kb on - strand at 2962.845 kb on - strand at 2962.845 kb on - strand at 2962.845 kb on - strand at 2962.845 kb on - strand at 2962.883 kb on + strand at 2962.884 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 2,960,260 + Pf1N1B4_2619 0.84 +0.0 2,960,260 + Pf1N1B4_2619 0.84 -1.3 2,960,261 - +0.0 2,960,261 - Pf1N1B4_2619 0.84 +0.0 2,960,261 - Pf1N1B4_2619 0.84 +0.0 2,960,261 - Pf1N1B4_2619 0.84 +0.0 2,960,261 - Pf1N1B4_2619 0.84 +0.0 2,960,266 + Pf1N1B4_2619 0.84 +0.0 2,960,266 + Pf1N1B4_2619 0.84 +0.0 2,960,266 + Pf1N1B4_2619 0.84 -1.5 2,960,266 + Pf1N1B4_2619 0.84 +0.0 2,960,267 - Pf1N1B4_2619 0.85 +0.0 2,960,267 - Pf1N1B4_2619 0.85 +0.0 2,960,267 - Pf1N1B4_2619 0.85 +0.0 2,960,267 - Pf1N1B4_2619 0.85 -0.5 2,960,267 - Pf1N1B4_2619 0.85 -3.7 2,960,267 - Pf1N1B4_2619 0.85 -3.5 2,960,270 + Pf1N1B4_2619 0.85 +0.0 2,960,271 - Pf1N1B4_2619 0.85 +0.0 2,960,323 + +0.0 2,960,323 + +0.0 2,960,323 + +0.0 2,960,324 - +0.0 2,960,324 - +0.0 2,960,324 - +0.0 2,960,324 - -1.2 2,960,334 + +0.0 2,960,334 + +0.0 2,960,334 + +0.0 2,960,334 + +0.0 2,960,334 + -1.9 2,960,334 + -1.5 2,960,334 + +0.0 2,960,334 + +0.0 2,960,334 + +0.0 2,960,334 + +0.0 2,960,334 + +0.0 2,960,335 - +0.0 2,960,335 - +0.0 2,960,335 - +0.0 2,960,335 - -2.3 2,960,335 - +0.0 2,960,335 - +0.0 2,960,335 - +0.0 2,960,340 + +0.0 2,960,340 + +0.0 2,960,340 + -2.3 2,960,340 + -3.0 2,960,340 + +0.0 2,960,341 - +0.0 2,960,341 - -2.2 2,960,341 - -1.3 2,960,341 - +0.0 2,960,398 + +0.0 2,960,398 + +0.0 2,960,398 + -2.7 2,960,398 + -1.3 2,960,399 - -3.5 2,960,399 - +0.0 2,960,399 - -2.1 2,960,399 - -0.4 2,960,409 + +0.0 2,960,409 + +0.0 2,960,409 + +0.0 2,960,409 + +0.0 2,960,410 - +0.0 2,960,427 + +0.0 2,960,427 + +0.0 2,960,427 + -1.9 2,960,427 + +0.0 2,960,427 + +0.0 2,960,427 + -2.7 2,960,427 + +0.0 2,960,427 + +0.0 2,960,427 + +0.0 2,960,427 + -1.4 2,960,428 - -3.0 2,960,428 - +0.0 2,960,428 - +0.0 2,960,463 + +0.0 2,960,510 + +0.0 2,960,510 + +0.0 2,960,528 + +0.0 2,960,532 + +0.0 2,960,533 - -2.6 2,960,538 + -1.0 2,960,552 + +0.0 2,960,552 + +0.0 2,960,576 + Pf1N1B4_2620 0.14 +0.0 2,960,576 + Pf1N1B4_2620 0.14 -1.8 2,960,576 + Pf1N1B4_2620 0.14 -0.5 2,960,576 + Pf1N1B4_2620 0.14 -2.8 2,960,576 + +0.0 2,960,576 + Pf1N1B4_2620 0.14 +0.0 2,960,576 + Pf1N1B4_2620 0.14 +0.0 2,960,577 - Pf1N1B4_2620 0.14 +0.0 2,960,577 - Pf1N1B4_2620 0.14 +0.0 2,960,577 - Pf1N1B4_2620 0.14 -1.9 2,960,577 - Pf1N1B4_2620 0.14 +0.0 2,960,632 + Pf1N1B4_2620 0.24 +0.0 2,960,632 + Pf1N1B4_2620 0.24 +0.0 2,960,633 - Pf1N1B4_2620 0.24 -3.8 2,960,633 - Pf1N1B4_2620 0.24 +0.0 2,960,705 - Pf1N1B4_2620 0.37 +0.0 2,960,705 - Pf1N1B4_2620 0.37 +0.0 2,960,792 + Pf1N1B4_2620 0.52 +0.0 2,960,793 - Pf1N1B4_2620 0.53 -2.0 2,960,798 + Pf1N1B4_2620 0.54 -1.7 2,960,799 - Pf1N1B4_2620 0.54 +0.0 2,960,846 + Pf1N1B4_2620 0.62 +0.0 2,960,966 + Pf1N1B4_2620 0.83 -2.0 2,960,967 - Pf1N1B4_2620 0.84 +0.0 2,960,975 - Pf1N1B4_2620 0.85 -2.8 2,961,084 + -2.2 2,961,469 - Pf1N1B4_2621 0.36 +0.0 2,961,712 + Pf1N1B4_2621 0.71 -2.9 2,961,713 - Pf1N1B4_2621 0.71 +0.0 2,961,920 - -1.3 2,961,941 - +0.0 2,962,058 - Pf1N1B4_2622 0.20 +0.0 2,962,058 - Pf1N1B4_2622 0.20 -1.4 2,962,058 - Pf1N1B4_2622 0.20 +0.0 2,962,150 - Pf1N1B4_2622 0.36 +0.0 2,962,220 - Pf1N1B4_2622 0.48 -1.3 2,962,220 - Pf1N1B4_2622 0.48 +0.0 2,962,220 - Pf1N1B4_2622 0.48 +0.0 2,962,220 - Pf1N1B4_2622 0.48 +0.0 2,962,336 + Pf1N1B4_2622 0.68 -1.9 2,962,337 - Pf1N1B4_2622 0.68 +0.0 2,962,337 - Pf1N1B4_2622 0.68 +0.0 2,962,337 - Pf1N1B4_2622 0.68 +0.0 2,962,359 - Pf1N1B4_2622 0.72 +0.0 2,962,359 - Pf1N1B4_2622 0.72 +0.0 2,962,362 - Pf1N1B4_2622 0.73 +0.0 2,962,362 - Pf1N1B4_2622 0.73 +0.0 2,962,375 + Pf1N1B4_2622 0.75 +0.0 2,962,376 - Pf1N1B4_2622 0.75 +0.0 2,962,376 - Pf1N1B4_2622 0.75 +0.0 2,962,483 + +0.0 2,962,484 - +0.0 2,962,484 - -1.6 2,962,484 - +0.0 2,962,520 + +0.0 2,962,520 + +0.0 2,962,520 + +0.0 2,962,521 - -2.2 2,962,522 + +0.0 2,962,522 + +0.0 2,962,522 + -2.1 2,962,522 + +0.0 2,962,522 + +0.0 2,962,522 + +0.7 2,962,522 + -1.0 2,962,523 - -5.0 2,962,523 - +0.0 2,962,529 + +0.0 2,962,529 + +0.0 2,962,530 - +0.0 2,962,530 - +0.0 2,962,530 - +0.0 2,962,530 - +0.0 2,962,629 - -1.9 2,962,629 - +0.0 2,962,670 + -0.4 2,962,695 + +0.0 2,962,695 + +0.0 2,962,774 + +0.0 2,962,774 + -2.2 2,962,774 + +0.0 2,962,775 - +0.0 2,962,793 + +0.0 2,962,793 + +0.0 2,962,844 + +0.0 2,962,844 + -2.0 2,962,844 + -3.1 2,962,844 + +0.0 2,962,845 - -2.1 2,962,845 - +0.0 2,962,845 - +0.0 2,962,845 - +0.0 2,962,845 - +0.0 2,962,845 - +0.0 2,962,883 + -1.4 2,962,884 - +0.0
Or see this region's nucleotide sequence