Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2553 and Pf1N1B4_2554 are separated by 184 nucleotides Pf1N1B4_2554 and Pf1N1B4_2555 are separated by 82 nucleotides Pf1N1B4_2555 and Pf1N1B4_2556 are separated by 50 nucleotides
Pf1N1B4_2553 - 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (EC 1.14.13.-), at 2,896,170 to 2,896,817
_2553
Pf1N1B4_2554 - Bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) (EC 3.6.1.17), at 2,897,002 to 2,897,340
_2554
Pf1N1B4_2555 - Oxidoreductase, short chain dehydrogenase/reductase family, at 2,897,423 to 2,898,223
_2555
Pf1N1B4_2556 - FIG00954311: hypothetical protein, at 2,898,274 to 2,899,251
_2556
Position (kb)
2897
2898
2899 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2896.851 kb on + strand at 2896.852 kb on - strand at 2896.852 kb on - strand at 2896.955 kb on + strand at 2896.955 kb on + strand at 2897.046 kb on + strand, within Pf1N1B4_2554 at 2897.046 kb on + strand, within Pf1N1B4_2554 at 2897.046 kb on + strand, within Pf1N1B4_2554 at 2897.047 kb on - strand, within Pf1N1B4_2554 at 2897.047 kb on - strand, within Pf1N1B4_2554 at 2897.047 kb on - strand at 2897.057 kb on + strand, within Pf1N1B4_2554 at 2897.058 kb on - strand, within Pf1N1B4_2554 at 2897.058 kb on - strand at 2897.058 kb on - strand, within Pf1N1B4_2554 at 2897.154 kb on + strand, within Pf1N1B4_2554 at 2897.154 kb on + strand, within Pf1N1B4_2554 at 2897.155 kb on - strand, within Pf1N1B4_2554 at 2897.173 kb on - strand, within Pf1N1B4_2554 at 2897.222 kb on - strand, within Pf1N1B4_2554 at 2897.234 kb on - strand, within Pf1N1B4_2554 at 2897.269 kb on + strand, within Pf1N1B4_2554 at 2897.285 kb on + strand, within Pf1N1B4_2554 at 2897.285 kb on + strand, within Pf1N1B4_2554 at 2897.285 kb on + strand, within Pf1N1B4_2554 at 2897.285 kb on + strand, within Pf1N1B4_2554 at 2897.285 kb on + strand, within Pf1N1B4_2554 at 2897.285 kb on + strand, within Pf1N1B4_2554 at 2897.285 kb on + strand, within Pf1N1B4_2554 at 2897.286 kb on - strand, within Pf1N1B4_2554 at 2897.286 kb on - strand, within Pf1N1B4_2554 at 2897.286 kb on - strand, within Pf1N1B4_2554 at 2897.286 kb on - strand, within Pf1N1B4_2554 at 2897.317 kb on - strand at 2897.317 kb on - strand at 2897.334 kb on + strand at 2897.377 kb on - strand at 2897.387 kb on + strand at 2897.436 kb on + strand at 2897.437 kb on - strand at 2897.437 kb on - strand at 2897.437 kb on - strand at 2897.437 kb on - strand at 2897.533 kb on + strand, within Pf1N1B4_2555 at 2897.533 kb on + strand at 2897.534 kb on - strand, within Pf1N1B4_2555 at 2897.534 kb on - strand, within Pf1N1B4_2555 at 2897.568 kb on + strand, within Pf1N1B4_2555 at 2897.568 kb on + strand, within Pf1N1B4_2555 at 2897.568 kb on + strand, within Pf1N1B4_2555 at 2897.568 kb on + strand, within Pf1N1B4_2555 at 2897.568 kb on + strand, within Pf1N1B4_2555 at 2897.569 kb on - strand, within Pf1N1B4_2555 at 2897.569 kb on - strand, within Pf1N1B4_2555 at 2897.569 kb on - strand, within Pf1N1B4_2555 at 2897.569 kb on - strand, within Pf1N1B4_2555 at 2897.569 kb on - strand, within Pf1N1B4_2555 at 2897.739 kb on + strand, within Pf1N1B4_2555 at 2897.739 kb on + strand, within Pf1N1B4_2555 at 2897.739 kb on + strand, within Pf1N1B4_2555 at 2897.739 kb on + strand, within Pf1N1B4_2555 at 2897.740 kb on - strand, within Pf1N1B4_2555 at 2897.740 kb on - strand, within Pf1N1B4_2555 at 2897.755 kb on - strand at 2897.757 kb on + strand, within Pf1N1B4_2555 at 2897.757 kb on + strand, within Pf1N1B4_2555 at 2897.757 kb on + strand, within Pf1N1B4_2555 at 2897.757 kb on + strand, within Pf1N1B4_2555 at 2897.757 kb on + strand, within Pf1N1B4_2555 at 2897.757 kb on + strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2898.292 kb on + strand at 2898.292 kb on + strand at 2898.293 kb on - strand at 2898.293 kb on - strand at 2898.401 kb on + strand, within Pf1N1B4_2556 at 2898.401 kb on + strand, within Pf1N1B4_2556 at 2898.401 kb on + strand, within Pf1N1B4_2556 at 2898.401 kb on + strand, within Pf1N1B4_2556 at 2898.401 kb on + strand, within Pf1N1B4_2556 at 2898.401 kb on + strand, within Pf1N1B4_2556 at 2898.401 kb on + strand, within Pf1N1B4_2556 at 2898.402 kb on - strand, within Pf1N1B4_2556 at 2898.402 kb on - strand, within Pf1N1B4_2556 at 2898.402 kb on - strand, within Pf1N1B4_2556 at 2898.402 kb on - strand, within Pf1N1B4_2556 at 2898.525 kb on + strand, within Pf1N1B4_2556 at 2898.583 kb on + strand, within Pf1N1B4_2556 at 2898.584 kb on - strand, within Pf1N1B4_2556 at 2898.586 kb on + strand, within Pf1N1B4_2556 at 2898.586 kb on + strand, within Pf1N1B4_2556 at 2898.586 kb on + strand at 2898.586 kb on + strand, within Pf1N1B4_2556 at 2898.586 kb on + strand, within Pf1N1B4_2556 at 2898.586 kb on + strand, within Pf1N1B4_2556 at 2898.587 kb on - strand, within Pf1N1B4_2556 at 2898.587 kb on - strand, within Pf1N1B4_2556 at 2898.762 kb on + strand, within Pf1N1B4_2556 at 2898.935 kb on + strand, within Pf1N1B4_2556 at 2898.935 kb on + strand, within Pf1N1B4_2556 at 2898.935 kb on + strand, within Pf1N1B4_2556 at 2898.936 kb on - strand, within Pf1N1B4_2556 at 2898.936 kb on - strand, within Pf1N1B4_2556 at 2899.030 kb on + strand, within Pf1N1B4_2556 at 2899.030 kb on + strand, within Pf1N1B4_2556 at 2899.070 kb on + strand, within Pf1N1B4_2556 at 2899.070 kb on + strand, within Pf1N1B4_2556 at 2899.070 kb on + strand, within Pf1N1B4_2556 at 2899.070 kb on + strand, within Pf1N1B4_2556 at 2899.070 kb on + strand, within Pf1N1B4_2556 at 2899.070 kb on + strand, within Pf1N1B4_2556 at 2899.071 kb on - strand at 2899.071 kb on - strand, within Pf1N1B4_2556 at 2899.071 kb on - strand, within Pf1N1B4_2556 at 2899.071 kb on - strand, within Pf1N1B4_2556
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 2,896,851 + -1.3 2,896,852 - -1.0 2,896,852 - +0.0 2,896,955 + +0.0 2,896,955 + +0.0 2,897,046 + Pf1N1B4_2554 0.13 -3.2 2,897,046 + Pf1N1B4_2554 0.13 +0.0 2,897,046 + Pf1N1B4_2554 0.13 -1.2 2,897,047 - Pf1N1B4_2554 0.13 +0.0 2,897,047 - Pf1N1B4_2554 0.13 +0.0 2,897,047 - +0.0 2,897,057 + Pf1N1B4_2554 0.16 -2.2 2,897,058 - Pf1N1B4_2554 0.17 +0.0 2,897,058 - -1.2 2,897,058 - Pf1N1B4_2554 0.17 +0.0 2,897,154 + Pf1N1B4_2554 0.45 -2.1 2,897,154 + Pf1N1B4_2554 0.45 -2.4 2,897,155 - Pf1N1B4_2554 0.45 +0.0 2,897,173 - Pf1N1B4_2554 0.50 +0.0 2,897,222 - Pf1N1B4_2554 0.65 +0.0 2,897,234 - Pf1N1B4_2554 0.68 -2.3 2,897,269 + Pf1N1B4_2554 0.79 -2.1 2,897,285 + Pf1N1B4_2554 0.83 -0.5 2,897,285 + Pf1N1B4_2554 0.83 +0.0 2,897,285 + Pf1N1B4_2554 0.83 +0.0 2,897,285 + Pf1N1B4_2554 0.83 -2.1 2,897,285 + Pf1N1B4_2554 0.83 -1.8 2,897,285 + Pf1N1B4_2554 0.83 +0.0 2,897,285 + Pf1N1B4_2554 0.83 +0.0 2,897,286 - Pf1N1B4_2554 0.84 +0.0 2,897,286 - Pf1N1B4_2554 0.84 +0.0 2,897,286 - Pf1N1B4_2554 0.84 +0.0 2,897,286 - Pf1N1B4_2554 0.84 +0.0 2,897,317 - +0.0 2,897,317 - +0.0 2,897,334 + +0.0 2,897,377 - -3.4 2,897,387 + -2.8 2,897,436 + +0.0 2,897,437 - +0.0 2,897,437 - +0.0 2,897,437 - +0.0 2,897,437 - -2.3 2,897,533 + Pf1N1B4_2555 0.14 -2.0 2,897,533 + +0.0 2,897,534 - Pf1N1B4_2555 0.14 +0.0 2,897,534 - Pf1N1B4_2555 0.14 -0.7 2,897,568 + Pf1N1B4_2555 0.18 +0.0 2,897,568 + Pf1N1B4_2555 0.18 +0.0 2,897,568 + Pf1N1B4_2555 0.18 -1.8 2,897,568 + Pf1N1B4_2555 0.18 +0.0 2,897,568 + Pf1N1B4_2555 0.18 -1.5 2,897,569 - Pf1N1B4_2555 0.18 +0.0 2,897,569 - Pf1N1B4_2555 0.18 +0.0 2,897,569 - Pf1N1B4_2555 0.18 +0.0 2,897,569 - Pf1N1B4_2555 0.18 -2.4 2,897,569 - Pf1N1B4_2555 0.18 -0.7 2,897,739 + Pf1N1B4_2555 0.39 +0.0 2,897,739 + Pf1N1B4_2555 0.39 -1.9 2,897,739 + Pf1N1B4_2555 0.39 +0.0 2,897,739 + Pf1N1B4_2555 0.39 +0.0 2,897,740 - Pf1N1B4_2555 0.40 +0.0 2,897,740 - Pf1N1B4_2555 0.40 +0.0 2,897,755 - -2.1 2,897,757 + Pf1N1B4_2555 0.42 +0.0 2,897,757 + Pf1N1B4_2555 0.42 -2.0 2,897,757 + Pf1N1B4_2555 0.42 -2.6 2,897,757 + Pf1N1B4_2555 0.42 -1.7 2,897,757 + Pf1N1B4_2555 0.42 +0.0 2,897,757 + Pf1N1B4_2555 0.42 +0.0 2,897,758 - Pf1N1B4_2555 0.42 +0.0 2,897,758 - Pf1N1B4_2555 0.42 +0.0 2,897,758 - Pf1N1B4_2555 0.42 +0.0 2,897,758 - Pf1N1B4_2555 0.42 +0.0 2,897,758 - Pf1N1B4_2555 0.42 +0.0 2,897,758 - Pf1N1B4_2555 0.42 +0.0 2,897,758 - Pf1N1B4_2555 0.42 -2.2 2,897,758 - Pf1N1B4_2555 0.42 +0.0 2,897,758 - Pf1N1B4_2555 0.42 -3.7 2,897,758 - Pf1N1B4_2555 0.42 -1.5 2,897,758 - Pf1N1B4_2555 0.42 -2.1 2,898,292 + +0.0 2,898,292 + -1.0 2,898,293 - -0.5 2,898,293 - +0.0 2,898,401 + Pf1N1B4_2556 0.13 +0.0 2,898,401 + Pf1N1B4_2556 0.13 +0.0 2,898,401 + Pf1N1B4_2556 0.13 +0.0 2,898,401 + Pf1N1B4_2556 0.13 +0.0 2,898,401 + Pf1N1B4_2556 0.13 +0.0 2,898,401 + Pf1N1B4_2556 0.13 -1.0 2,898,401 + Pf1N1B4_2556 0.13 +0.0 2,898,402 - Pf1N1B4_2556 0.13 -1.3 2,898,402 - Pf1N1B4_2556 0.13 +0.0 2,898,402 - Pf1N1B4_2556 0.13 +0.0 2,898,402 - Pf1N1B4_2556 0.13 -0.8 2,898,525 + Pf1N1B4_2556 0.26 +0.0 2,898,583 + Pf1N1B4_2556 0.32 +0.0 2,898,584 - Pf1N1B4_2556 0.32 +0.0 2,898,586 + Pf1N1B4_2556 0.32 +0.0 2,898,586 + Pf1N1B4_2556 0.32 +0.5 2,898,586 + -2.7 2,898,586 + Pf1N1B4_2556 0.32 +0.0 2,898,586 + Pf1N1B4_2556 0.32 +0.0 2,898,586 + Pf1N1B4_2556 0.32 +0.0 2,898,587 - Pf1N1B4_2556 0.32 -0.8 2,898,587 - Pf1N1B4_2556 0.32 -1.8 2,898,762 + Pf1N1B4_2556 0.50 +0.0 2,898,935 + Pf1N1B4_2556 0.68 +0.0 2,898,935 + Pf1N1B4_2556 0.68 +0.0 2,898,935 + Pf1N1B4_2556 0.68 +0.0 2,898,936 - Pf1N1B4_2556 0.68 -1.8 2,898,936 - Pf1N1B4_2556 0.68 +0.0 2,899,030 + Pf1N1B4_2556 0.77 -2.3 2,899,030 + Pf1N1B4_2556 0.77 -0.9 2,899,070 + Pf1N1B4_2556 0.81 +0.0 2,899,070 + Pf1N1B4_2556 0.81 +0.0 2,899,070 + Pf1N1B4_2556 0.81 +0.0 2,899,070 + Pf1N1B4_2556 0.81 -3.0 2,899,070 + Pf1N1B4_2556 0.81 +0.0 2,899,070 + Pf1N1B4_2556 0.81 +0.0 2,899,071 - -1.8 2,899,071 - Pf1N1B4_2556 0.81 -1.9 2,899,071 - Pf1N1B4_2556 0.81 +0.0 2,899,071 - Pf1N1B4_2556 0.81 +0.0
Or see this region's nucleotide sequence