Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_195 and Pf1N1B4_196 are separated by 335 nucleotides Pf1N1B4_196 and Pf1N1B4_197 overlap by 4 nucleotides Pf1N1B4_197 and Pf1N1B4_198 overlap by 4 nucleotides Pf1N1B4_198 and Pf1N1B4_199 overlap by 4 nucleotides
Pf1N1B4_195 - PhnB protein; putative DNA binding 3-demethylubiquinone-9 3-methyltransferase domain protein, at 243,777 to 244,235
_195
Pf1N1B4_196 - FIG137594: Putative iron-regulated membrane protein, at 244,571 to 245,776
_196
Pf1N1B4_197 - FIG139991: Putative thiamine pyrophosphate-requiring enzyme, at 245,773 to 246,315
_197
Pf1N1B4_198 - FIG137877: Hypothetical protein in pyoverdin gene cluster, at 246,312 to 246,632
_198
Pf1N1B4_199 - FIG049111: Hypothetical protein in pyoverdin gene cluster, at 246,629 to 247,204
_199
Position (kb)
244
245
246 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 243.617 kb on + strand at 243.713 kb on + strand at 243.713 kb on + strand at 243.713 kb on + strand at 243.713 kb on + strand at 243.713 kb on + strand at 243.713 kb on + strand at 243.713 kb on + strand at 243.715 kb on + strand at 243.715 kb on + strand at 243.715 kb on + strand at 243.715 kb on + strand at 243.715 kb on + strand at 243.719 kb on + strand at 243.719 kb on + strand at 243.784 kb on + strand at 243.804 kb on + strand at 243.822 kb on + strand at 243.826 kb on + strand, within Pf1N1B4_195 at 243.826 kb on + strand, within Pf1N1B4_195 at 243.938 kb on + strand, within Pf1N1B4_195 at 243.938 kb on + strand, within Pf1N1B4_195 at 243.939 kb on - strand, within Pf1N1B4_195 at 244.029 kb on + strand, within Pf1N1B4_195 at 244.029 kb on + strand, within Pf1N1B4_195 at 244.029 kb on + strand, within Pf1N1B4_195 at 244.029 kb on + strand, within Pf1N1B4_195 at 244.030 kb on - strand, within Pf1N1B4_195 at 244.030 kb on - strand, within Pf1N1B4_195 at 244.030 kb on - strand, within Pf1N1B4_195 at 244.030 kb on - strand, within Pf1N1B4_195 at 244.055 kb on + strand, within Pf1N1B4_195 at 244.111 kb on - strand, within Pf1N1B4_195 at 244.153 kb on + strand, within Pf1N1B4_195 at 244.153 kb on + strand at 244.153 kb on + strand, within Pf1N1B4_195 at 244.153 kb on + strand at 244.153 kb on + strand, within Pf1N1B4_195 at 244.153 kb on + strand, within Pf1N1B4_195 at 244.153 kb on + strand, within Pf1N1B4_195 at 244.154 kb on - strand, within Pf1N1B4_195 at 244.154 kb on - strand, within Pf1N1B4_195 at 244.223 kb on + strand at 244.224 kb on - strand at 244.224 kb on - strand at 244.260 kb on + strand at 244.261 kb on - strand at 244.295 kb on + strand at 244.295 kb on + strand at 244.400 kb on + strand at 244.418 kb on - strand at 244.453 kb on + strand at 244.454 kb on - strand at 244.464 kb on + strand at 244.567 kb on - strand at 244.612 kb on + strand at 244.613 kb on - strand at 244.644 kb on + strand at 244.757 kb on + strand, within Pf1N1B4_196 at 244.758 kb on - strand, within Pf1N1B4_196 at 244.889 kb on + strand, within Pf1N1B4_196 at 244.889 kb on + strand, within Pf1N1B4_196 at 244.889 kb on + strand, within Pf1N1B4_196 at 244.890 kb on - strand at 244.890 kb on - strand, within Pf1N1B4_196 at 244.890 kb on - strand, within Pf1N1B4_196 at 245.255 kb on - strand, within Pf1N1B4_196 at 245.414 kb on + strand, within Pf1N1B4_196 at 245.414 kb on + strand, within Pf1N1B4_196 at 245.414 kb on + strand, within Pf1N1B4_196 at 245.483 kb on + strand, within Pf1N1B4_196 at 245.483 kb on + strand, within Pf1N1B4_196 at 245.484 kb on - strand, within Pf1N1B4_196 at 245.887 kb on + strand, within Pf1N1B4_197 at 245.888 kb on - strand, within Pf1N1B4_197 at 245.888 kb on - strand, within Pf1N1B4_197 at 246.025 kb on + strand, within Pf1N1B4_197 at 246.025 kb on + strand at 246.337 kb on + strand at 246.338 kb on - strand at 246.338 kb on - strand at 246.338 kb on - strand at 246.341 kb on - strand at 246.564 kb on + strand, within Pf1N1B4_198 at 246.564 kb on + strand, within Pf1N1B4_198 at 246.564 kb on + strand at 246.564 kb on + strand, within Pf1N1B4_198 at 246.565 kb on - strand, within Pf1N1B4_198 at 246.565 kb on - strand, within Pf1N1B4_198 at 246.565 kb on - strand, within Pf1N1B4_198 at 246.565 kb on - strand, within Pf1N1B4_198 at 246.634 kb on + strand at 246.634 kb on + strand at 246.635 kb on - strand at 246.635 kb on - strand at 246.635 kb on - strand at 246.686 kb on + strand at 246.686 kb on + strand at 246.686 kb on + strand at 246.686 kb on + strand at 246.686 kb on + strand at 246.686 kb on + strand at 246.686 kb on + strand at 246.687 kb on - strand, within Pf1N1B4_199 at 246.687 kb on - strand, within Pf1N1B4_199 at 246.687 kb on - strand, within Pf1N1B4_199 at 246.687 kb on - strand, within Pf1N1B4_199 at 246.687 kb on - strand, within Pf1N1B4_199 at 246.687 kb on - strand, within Pf1N1B4_199 at 246.687 kb on - strand, within Pf1N1B4_199
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 243,617 + -0.0 243,713 + -0.0 243,713 + -0.0 243,713 + -1.8 243,713 + -0.0 243,713 + -0.0 243,713 + -0.0 243,713 + -2.3 243,715 + -2.6 243,715 + -0.0 243,715 + -0.0 243,715 + -1.1 243,715 + -2.5 243,719 + -0.0 243,719 + -0.0 243,784 + -0.0 243,804 + -1.4 243,822 + -0.0 243,826 + Pf1N1B4_195 0.11 -3.6 243,826 + Pf1N1B4_195 0.11 -0.0 243,938 + Pf1N1B4_195 0.35 -0.0 243,938 + Pf1N1B4_195 0.35 -0.0 243,939 - Pf1N1B4_195 0.35 -0.0 244,029 + Pf1N1B4_195 0.55 -0.0 244,029 + Pf1N1B4_195 0.55 -0.7 244,029 + Pf1N1B4_195 0.55 -3.9 244,029 + Pf1N1B4_195 0.55 -0.0 244,030 - Pf1N1B4_195 0.55 -0.0 244,030 - Pf1N1B4_195 0.55 -0.0 244,030 - Pf1N1B4_195 0.55 -2.3 244,030 - Pf1N1B4_195 0.55 -0.0 244,055 + Pf1N1B4_195 0.61 -0.0 244,111 - Pf1N1B4_195 0.73 -0.0 244,153 + Pf1N1B4_195 0.82 -2.0 244,153 + -0.0 244,153 + Pf1N1B4_195 0.82 -0.0 244,153 + -0.0 244,153 + Pf1N1B4_195 0.82 -2.0 244,153 + Pf1N1B4_195 0.82 -1.9 244,153 + Pf1N1B4_195 0.82 -0.0 244,154 - Pf1N1B4_195 0.82 -1.3 244,154 - Pf1N1B4_195 0.82 -0.0 244,223 + -3.0 244,224 - -3.9 244,224 - -1.7 244,260 + -1.6 244,261 - -0.0 244,295 + -2.7 244,295 + -0.0 244,400 + -0.0 244,418 - -0.0 244,453 + -0.0 244,454 - -0.0 244,464 + -0.0 244,567 - -0.0 244,612 + -0.0 244,613 - -1.2 244,644 + -0.0 244,757 + Pf1N1B4_196 0.15 -2.8 244,758 - Pf1N1B4_196 0.16 -0.0 244,889 + Pf1N1B4_196 0.26 -2.4 244,889 + Pf1N1B4_196 0.26 -0.0 244,889 + Pf1N1B4_196 0.26 -0.0 244,890 - -0.0 244,890 - Pf1N1B4_196 0.26 -0.0 244,890 - Pf1N1B4_196 0.26 -0.0 245,255 - Pf1N1B4_196 0.57 -2.3 245,414 + Pf1N1B4_196 0.70 -2.6 245,414 + Pf1N1B4_196 0.70 -0.0 245,414 + Pf1N1B4_196 0.70 -2.3 245,483 + Pf1N1B4_196 0.76 -0.5 245,483 + Pf1N1B4_196 0.76 -0.0 245,484 - Pf1N1B4_196 0.76 -0.0 245,887 + Pf1N1B4_197 0.21 -0.0 245,888 - Pf1N1B4_197 0.21 -1.0 245,888 - Pf1N1B4_197 0.21 -0.0 246,025 + Pf1N1B4_197 0.46 -0.0 246,025 + -0.0 246,337 + -0.0 246,338 - -1.6 246,338 - -0.8 246,338 - -2.4 246,341 - -0.0 246,564 + Pf1N1B4_198 0.79 -4.8 246,564 + Pf1N1B4_198 0.79 -0.0 246,564 + -0.0 246,564 + Pf1N1B4_198 0.79 -0.0 246,565 - Pf1N1B4_198 0.79 -0.0 246,565 - Pf1N1B4_198 0.79 -0.0 246,565 - Pf1N1B4_198 0.79 -0.0 246,565 - Pf1N1B4_198 0.79 -0.0 246,634 + -0.0 246,634 + -1.7 246,635 - -1.6 246,635 - -0.0 246,635 - -0.0 246,686 + -1.4 246,686 + -0.0 246,686 + -0.0 246,686 + -3.7 246,686 + -0.0 246,686 + -0.0 246,686 + -1.3 246,687 - Pf1N1B4_199 0.10 -0.0 246,687 - Pf1N1B4_199 0.10 -0.0 246,687 - Pf1N1B4_199 0.10 -2.9 246,687 - Pf1N1B4_199 0.10 -0.0 246,687 - Pf1N1B4_199 0.10 -0.0 246,687 - Pf1N1B4_199 0.10 -0.0 246,687 - Pf1N1B4_199 0.10 -0.0
Or see this region's nucleotide sequence