Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_182

Experiment: Nitrate reduction

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_181 and Pf1N1B4_182 overlap by 4 nucleotidesPf1N1B4_182 and Pf1N1B4_183 overlap by 4 nucleotides Pf1N1B4_181 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 224,292 to 225,233 _181 Pf1N1B4_182 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 225,230 to 225,985 _182 Pf1N1B4_183 - Major facilitator family transporter, at 225,982 to 227,283 _183 Position (kb) 225 226Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 224.249 kb on - strandat 224.294 kb on - strandat 224.364 kb on + strandat 224.707 kb on - strand, within Pf1N1B4_181at 224.926 kb on + strand, within Pf1N1B4_181at 224.927 kb on - strand, within Pf1N1B4_181at 225.240 kb on + strandat 225.240 kb on + strandat 225.240 kb on + strandat 225.240 kb on + strandat 225.241 kb on - strandat 225.241 kb on - strandat 225.334 kb on + strandat 225.335 kb on - strand, within Pf1N1B4_182at 225.558 kb on + strandat 225.558 kb on + strand, within Pf1N1B4_182at 225.558 kb on + strand, within Pf1N1B4_182at 225.558 kb on + strand, within Pf1N1B4_182at 225.558 kb on + strand, within Pf1N1B4_182at 225.558 kb on + strand, within Pf1N1B4_182at 225.559 kb on - strand, within Pf1N1B4_182at 225.559 kb on - strand, within Pf1N1B4_182at 225.559 kb on - strandat 225.559 kb on - strand, within Pf1N1B4_182at 225.559 kb on - strandat 225.748 kb on - strand, within Pf1N1B4_182at 225.748 kb on - strand, within Pf1N1B4_182at 225.875 kb on - strand, within Pf1N1B4_182at 225.980 kb on - strandat 225.980 kb on - strandat 225.989 kb on + strandat 226.236 kb on - strand, within Pf1N1B4_183at 226.253 kb on + strand, within Pf1N1B4_183at 226.494 kb on - strand, within Pf1N1B4_183at 226.505 kb on + strand, within Pf1N1B4_183at 226.505 kb on + strand, within Pf1N1B4_183at 226.506 kb on - strand, within Pf1N1B4_183at 226.506 kb on - strand, within Pf1N1B4_183at 226.839 kb on - strand, within Pf1N1B4_183at 226.839 kb on - strandat 226.960 kb on - strand, within Pf1N1B4_183

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction
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224,249 - -0.0
224,294 - -0.0
224,364 + -0.0
224,707 - Pf1N1B4_181 0.44 -0.0
224,926 + Pf1N1B4_181 0.67 -2.5
224,927 - Pf1N1B4_181 0.67 -0.0
225,240 + -1.9
225,240 + -0.0
225,240 + -0.0
225,240 + -0.0
225,241 - -0.0
225,241 - -0.0
225,334 + -0.0
225,335 - Pf1N1B4_182 0.14 -0.0
225,558 + -0.0
225,558 + Pf1N1B4_182 0.43 -2.7
225,558 + Pf1N1B4_182 0.43 -0.0
225,558 + Pf1N1B4_182 0.43 -0.0
225,558 + Pf1N1B4_182 0.43 -2.0
225,558 + Pf1N1B4_182 0.43 -0.0
225,559 - Pf1N1B4_182 0.44 -0.0
225,559 - Pf1N1B4_182 0.44 -0.0
225,559 - -3.9
225,559 - Pf1N1B4_182 0.44 -4.7
225,559 - -1.9
225,748 - Pf1N1B4_182 0.69 -0.0
225,748 - Pf1N1B4_182 0.69 -0.0
225,875 - Pf1N1B4_182 0.85 -0.0
225,980 - -0.0
225,980 - -0.0
225,989 + -2.4
226,236 - Pf1N1B4_183 0.20 -0.0
226,253 + Pf1N1B4_183 0.21 -1.6
226,494 - Pf1N1B4_183 0.39 -2.2
226,505 + Pf1N1B4_183 0.40 -0.0
226,505 + Pf1N1B4_183 0.40 -1.6
226,506 - Pf1N1B4_183 0.40 -2.1
226,506 - Pf1N1B4_183 0.40 -2.4
226,839 - Pf1N1B4_183 0.66 -2.7
226,839 - -0.0
226,960 - Pf1N1B4_183 0.75 -0.0

Or see this region's nucleotide sequence