Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1724 and Pf1N1B4_1725 are separated by 3 nucleotides Pf1N1B4_1725 and Pf1N1B4_1726 are separated by 85 nucleotides Pf1N1B4_1726 and Pf1N1B4_1727 are separated by 204 nucleotides
Pf1N1B4_1724 - Sulfate and thiosulfate import ATP-binding protein CysA (EC 3.6.3.25), at 1,970,644 to 1,971,633
_1724
Pf1N1B4_1725 - Sulfate transport system permease protein CysW, at 1,971,637 to 1,972,434
_1725
Pf1N1B4_1726 - Sulfate transport system permease protein CysT, at 1,972,520 to 1,973,338
_1726
Pf1N1B4_1727 - Sulfate and thiosulfate binding protein CysP, at 1,973,543 to 1,974,556
_1727
Position (kb)
1972
1973
1974 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1971.638 kb on + strand at 1971.638 kb on + strand at 1971.638 kb on + strand at 1971.638 kb on + strand at 1971.638 kb on + strand at 1971.638 kb on + strand at 1971.639 kb on - strand at 1971.639 kb on - strand at 1971.639 kb on - strand at 1971.639 kb on - strand at 1971.639 kb on - strand at 1971.673 kb on - strand at 1971.761 kb on + strand, within Pf1N1B4_1725 at 1971.761 kb on + strand, within Pf1N1B4_1725 at 1971.761 kb on + strand, within Pf1N1B4_1725 at 1971.762 kb on - strand, within Pf1N1B4_1725 at 1971.762 kb on - strand, within Pf1N1B4_1725 at 1971.762 kb on - strand, within Pf1N1B4_1725 at 1971.770 kb on + strand, within Pf1N1B4_1725 at 1971.770 kb on + strand, within Pf1N1B4_1725 at 1971.770 kb on + strand, within Pf1N1B4_1725 at 1971.770 kb on + strand, within Pf1N1B4_1725 at 1971.771 kb on - strand, within Pf1N1B4_1725 at 1971.771 kb on - strand, within Pf1N1B4_1725 at 1971.771 kb on - strand, within Pf1N1B4_1725 at 1971.771 kb on - strand, within Pf1N1B4_1725 at 1971.771 kb on - strand, within Pf1N1B4_1725 at 1971.884 kb on + strand, within Pf1N1B4_1725 at 1971.884 kb on + strand, within Pf1N1B4_1725 at 1971.884 kb on + strand, within Pf1N1B4_1725 at 1971.884 kb on + strand, within Pf1N1B4_1725 at 1971.884 kb on + strand, within Pf1N1B4_1725 at 1971.885 kb on - strand, within Pf1N1B4_1725 at 1971.885 kb on - strand, within Pf1N1B4_1725 at 1971.885 kb on - strand, within Pf1N1B4_1725 at 1972.132 kb on + strand, within Pf1N1B4_1725 at 1972.132 kb on + strand, within Pf1N1B4_1725 at 1972.132 kb on + strand, within Pf1N1B4_1725 at 1972.132 kb on + strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.217 kb on + strand, within Pf1N1B4_1725 at 1972.218 kb on - strand, within Pf1N1B4_1725 at 1972.218 kb on - strand, within Pf1N1B4_1725 at 1972.491 kb on + strand at 1972.491 kb on + strand at 1972.492 kb on - strand at 1972.592 kb on + strand at 1972.592 kb on + strand at 1972.592 kb on + strand at 1972.593 kb on - strand at 1972.593 kb on - strand at 1972.617 kb on + strand, within Pf1N1B4_1726 at 1972.618 kb on - strand, within Pf1N1B4_1726 at 1972.623 kb on + strand, within Pf1N1B4_1726 at 1972.624 kb on - strand, within Pf1N1B4_1726 at 1972.797 kb on - strand, within Pf1N1B4_1726 at 1972.856 kb on + strand, within Pf1N1B4_1726 at 1972.870 kb on + strand, within Pf1N1B4_1726 at 1972.871 kb on - strand, within Pf1N1B4_1726 at 1972.871 kb on - strand at 1972.871 kb on - strand, within Pf1N1B4_1726 at 1972.874 kb on + strand, within Pf1N1B4_1726 at 1972.874 kb on + strand, within Pf1N1B4_1726 at 1972.875 kb on - strand, within Pf1N1B4_1726 at 1972.878 kb on - strand, within Pf1N1B4_1726 at 1972.878 kb on - strand, within Pf1N1B4_1726 at 1972.892 kb on + strand, within Pf1N1B4_1726 at 1972.892 kb on + strand, within Pf1N1B4_1726 at 1972.929 kb on + strand, within Pf1N1B4_1726 at 1972.929 kb on + strand, within Pf1N1B4_1726 at 1972.986 kb on + strand, within Pf1N1B4_1726 at 1972.986 kb on + strand, within Pf1N1B4_1726 at 1972.987 kb on - strand, within Pf1N1B4_1726 at 1973.079 kb on + strand, within Pf1N1B4_1726 at 1973.136 kb on + strand, within Pf1N1B4_1726 at 1973.137 kb on - strand, within Pf1N1B4_1726 at 1973.137 kb on - strand, within Pf1N1B4_1726 at 1973.216 kb on - strand, within Pf1N1B4_1726 at 1973.274 kb on + strand at 1973.274 kb on + strand at 1973.274 kb on + strand at 1973.275 kb on - strand at 1973.275 kb on - strand at 1973.275 kb on - strand at 1973.275 kb on - strand at 1973.275 kb on - strand at 1973.275 kb on - strand at 1973.275 kb on - strand at 1973.286 kb on + strand at 1973.326 kb on + strand at 1973.327 kb on - strand at 1973.327 kb on - strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.338 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.410 kb on + strand at 1973.411 kb on - strand at 1973.411 kb on - strand at 1973.411 kb on - strand at 1973.411 kb on - strand at 1973.411 kb on - strand at 1973.411 kb on - strand at 1973.411 kb on - strand at 1973.411 kb on - strand at 1973.418 kb on - strand at 1973.534 kb on + strand at 1973.534 kb on + strand at 1973.534 kb on + strand at 1973.535 kb on - strand at 1973.535 kb on - strand at 1973.535 kb on - strand at 1973.535 kb on - strand at 1973.535 kb on - strand at 1973.556 kb on + strand at 1973.556 kb on + strand at 1973.557 kb on - strand at 1973.557 kb on - strand at 1973.557 kb on - strand at 1973.700 kb on + strand, within Pf1N1B4_1727 at 1973.701 kb on - strand, within Pf1N1B4_1727 at 1973.740 kb on - strand, within Pf1N1B4_1727 at 1973.740 kb on - strand, within Pf1N1B4_1727 at 1973.754 kb on + strand, within Pf1N1B4_1727 at 1974.009 kb on + strand, within Pf1N1B4_1727 at 1974.009 kb on + strand, within Pf1N1B4_1727 at 1974.009 kb on + strand, within Pf1N1B4_1727 at 1974.009 kb on + strand, within Pf1N1B4_1727 at 1974.009 kb on + strand, within Pf1N1B4_1727 at 1974.009 kb on + strand, within Pf1N1B4_1727 at 1974.010 kb on - strand, within Pf1N1B4_1727 at 1974.010 kb on - strand, within Pf1N1B4_1727 at 1974.010 kb on - strand, within Pf1N1B4_1727 at 1974.054 kb on + strand, within Pf1N1B4_1727 at 1974.055 kb on - strand, within Pf1N1B4_1727 at 1974.204 kb on + strand, within Pf1N1B4_1727 at 1974.204 kb on + strand, within Pf1N1B4_1727 at 1974.204 kb on + strand, within Pf1N1B4_1727 at 1974.204 kb on + strand, within Pf1N1B4_1727 at 1974.204 kb on + strand, within Pf1N1B4_1727 at 1974.205 kb on - strand, within Pf1N1B4_1727
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 1,971,638 + +0.0 1,971,638 + +0.0 1,971,638 + +0.0 1,971,638 + +0.0 1,971,638 + +0.0 1,971,638 + +0.0 1,971,639 - +0.0 1,971,639 - +0.0 1,971,639 - +0.0 1,971,639 - +0.0 1,971,639 - +0.0 1,971,673 - +0.0 1,971,761 + Pf1N1B4_1725 0.16 -4.8 1,971,761 + Pf1N1B4_1725 0.16 +0.0 1,971,761 + Pf1N1B4_1725 0.16 -1.1 1,971,762 - Pf1N1B4_1725 0.16 +0.0 1,971,762 - Pf1N1B4_1725 0.16 +0.0 1,971,762 - Pf1N1B4_1725 0.16 +0.0 1,971,770 + Pf1N1B4_1725 0.17 -3.9 1,971,770 + Pf1N1B4_1725 0.17 +0.0 1,971,770 + Pf1N1B4_1725 0.17 -3.1 1,971,770 + Pf1N1B4_1725 0.17 -3.9 1,971,771 - Pf1N1B4_1725 0.17 +0.0 1,971,771 - Pf1N1B4_1725 0.17 -3.0 1,971,771 - Pf1N1B4_1725 0.17 +0.0 1,971,771 - Pf1N1B4_1725 0.17 +0.0 1,971,771 - Pf1N1B4_1725 0.17 +0.0 1,971,884 + Pf1N1B4_1725 0.31 +0.0 1,971,884 + Pf1N1B4_1725 0.31 -3.4 1,971,884 + Pf1N1B4_1725 0.31 -4.3 1,971,884 + Pf1N1B4_1725 0.31 -5.8 1,971,884 + Pf1N1B4_1725 0.31 +0.0 1,971,885 - Pf1N1B4_1725 0.31 +0.0 1,971,885 - Pf1N1B4_1725 0.31 +0.0 1,971,885 - Pf1N1B4_1725 0.31 +0.0 1,972,132 + Pf1N1B4_1725 0.62 +0.0 1,972,132 + Pf1N1B4_1725 0.62 +0.0 1,972,132 + Pf1N1B4_1725 0.62 +0.0 1,972,132 + Pf1N1B4_1725 0.62 -5.3 1,972,133 - Pf1N1B4_1725 0.62 +0.0 1,972,133 - Pf1N1B4_1725 0.62 -3.0 1,972,133 - Pf1N1B4_1725 0.62 +0.0 1,972,133 - Pf1N1B4_1725 0.62 -2.4 1,972,133 - Pf1N1B4_1725 0.62 +0.0 1,972,133 - Pf1N1B4_1725 0.62 -3.6 1,972,133 - Pf1N1B4_1725 0.62 +0.0 1,972,133 - Pf1N1B4_1725 0.62 -3.4 1,972,133 - Pf1N1B4_1725 0.62 -3.0 1,972,133 - +0.0 1,972,133 - Pf1N1B4_1725 0.62 -3.8 1,972,133 - Pf1N1B4_1725 0.62 -4.3 1,972,133 - Pf1N1B4_1725 0.62 +0.0 1,972,133 - Pf1N1B4_1725 0.62 +0.0 1,972,133 - Pf1N1B4_1725 0.62 +0.0 1,972,133 - Pf1N1B4_1725 0.62 -4.5 1,972,133 - Pf1N1B4_1725 0.62 -4.0 1,972,133 - Pf1N1B4_1725 0.62 +0.0 1,972,133 - Pf1N1B4_1725 0.62 -4.3 1,972,136 + Pf1N1B4_1725 0.63 +0.0 1,972,136 + Pf1N1B4_1725 0.63 -4.1 1,972,136 + Pf1N1B4_1725 0.63 +0.0 1,972,136 + Pf1N1B4_1725 0.63 +0.0 1,972,136 + Pf1N1B4_1725 0.63 +0.0 1,972,136 + Pf1N1B4_1725 0.63 +0.0 1,972,136 + Pf1N1B4_1725 0.63 +0.0 1,972,136 + Pf1N1B4_1725 0.63 -4.1 1,972,136 + Pf1N1B4_1725 0.63 +0.0 1,972,137 - Pf1N1B4_1725 0.63 +0.0 1,972,137 - Pf1N1B4_1725 0.63 +0.0 1,972,137 - Pf1N1B4_1725 0.63 +0.0 1,972,137 - Pf1N1B4_1725 0.63 -3.6 1,972,137 - Pf1N1B4_1725 0.63 +0.0 1,972,137 - Pf1N1B4_1725 0.63 -4.5 1,972,137 - Pf1N1B4_1725 0.63 +0.0 1,972,137 - Pf1N1B4_1725 0.63 +0.0 1,972,137 - Pf1N1B4_1725 0.63 -3.0 1,972,137 - Pf1N1B4_1725 0.63 -3.5 1,972,217 + Pf1N1B4_1725 0.73 +0.0 1,972,218 - Pf1N1B4_1725 0.73 +0.0 1,972,218 - Pf1N1B4_1725 0.73 -3.0 1,972,491 + +0.0 1,972,491 + +0.0 1,972,492 - +0.0 1,972,592 + +0.0 1,972,592 + +0.0 1,972,592 + +0.0 1,972,593 - -2.5 1,972,593 - +0.0 1,972,617 + Pf1N1B4_1726 0.12 +0.0 1,972,618 - Pf1N1B4_1726 0.12 +0.0 1,972,623 + Pf1N1B4_1726 0.13 +0.0 1,972,624 - Pf1N1B4_1726 0.13 -3.7 1,972,797 - Pf1N1B4_1726 0.34 +0.0 1,972,856 + Pf1N1B4_1726 0.41 +0.0 1,972,870 + Pf1N1B4_1726 0.43 +0.0 1,972,871 - Pf1N1B4_1726 0.43 +0.0 1,972,871 - +0.0 1,972,871 - Pf1N1B4_1726 0.43 -3.7 1,972,874 + Pf1N1B4_1726 0.43 +0.0 1,972,874 + Pf1N1B4_1726 0.43 +0.0 1,972,875 - Pf1N1B4_1726 0.43 +0.0 1,972,878 - Pf1N1B4_1726 0.44 +0.0 1,972,878 - Pf1N1B4_1726 0.44 +0.0 1,972,892 + Pf1N1B4_1726 0.45 +0.0 1,972,892 + Pf1N1B4_1726 0.45 +0.0 1,972,929 + Pf1N1B4_1726 0.50 +0.0 1,972,929 + Pf1N1B4_1726 0.50 +0.0 1,972,986 + Pf1N1B4_1726 0.57 +0.0 1,972,986 + Pf1N1B4_1726 0.57 -5.7 1,972,987 - Pf1N1B4_1726 0.57 -3.3 1,973,079 + Pf1N1B4_1726 0.68 -5.4 1,973,136 + Pf1N1B4_1726 0.75 -3.5 1,973,137 - Pf1N1B4_1726 0.75 +0.0 1,973,137 - Pf1N1B4_1726 0.75 +0.0 1,973,216 - Pf1N1B4_1726 0.85 -1.7 1,973,274 + +0.0 1,973,274 + +0.0 1,973,274 + -3.1 1,973,275 - +0.0 1,973,275 - +0.0 1,973,275 - -6.4 1,973,275 - +0.0 1,973,275 - +0.0 1,973,275 - +0.0 1,973,275 - +0.0 1,973,286 + +0.0 1,973,326 + +0.0 1,973,327 - +0.0 1,973,327 - +0.0 1,973,338 + +0.0 1,973,338 + +0.0 1,973,338 + +0.0 1,973,338 + -4.4 1,973,338 + -4.1 1,973,338 + +0.0 1,973,338 + +0.0 1,973,338 + +0.0 1,973,338 + -3.8 1,973,338 + +0.0 1,973,338 + +0.0 1,973,338 + +0.0 1,973,410 + +0.0 1,973,410 + +0.0 1,973,410 + +0.0 1,973,410 + -4.5 1,973,410 + +0.0 1,973,410 + +0.0 1,973,410 + -2.8 1,973,410 + +0.0 1,973,410 + +0.0 1,973,410 + +0.0 1,973,410 + +0.0 1,973,410 + +0.0 1,973,410 + -2.1 1,973,410 + +0.0 1,973,411 - -1.1 1,973,411 - +0.0 1,973,411 - +0.0 1,973,411 - -1.8 1,973,411 - +0.0 1,973,411 - -3.6 1,973,411 - +0.0 1,973,411 - +0.0 1,973,418 - +0.0 1,973,534 + +0.0 1,973,534 + -3.6 1,973,534 + -2.9 1,973,535 - -1.3 1,973,535 - -2.7 1,973,535 - -1.1 1,973,535 - -1.9 1,973,535 - +0.0 1,973,556 + +0.0 1,973,556 + +0.0 1,973,557 - +0.0 1,973,557 - +0.0 1,973,557 - +0.0 1,973,700 + Pf1N1B4_1727 0.15 +0.0 1,973,701 - Pf1N1B4_1727 0.16 +0.0 1,973,740 - Pf1N1B4_1727 0.19 -0.9 1,973,740 - Pf1N1B4_1727 0.19 +0.0 1,973,754 + Pf1N1B4_1727 0.21 +0.0 1,974,009 + Pf1N1B4_1727 0.46 +0.0 1,974,009 + Pf1N1B4_1727 0.46 +0.0 1,974,009 + Pf1N1B4_1727 0.46 -4.1 1,974,009 + Pf1N1B4_1727 0.46 -1.7 1,974,009 + Pf1N1B4_1727 0.46 +0.0 1,974,009 + Pf1N1B4_1727 0.46 +0.0 1,974,010 - Pf1N1B4_1727 0.46 +0.0 1,974,010 - Pf1N1B4_1727 0.46 +0.0 1,974,010 - Pf1N1B4_1727 0.46 -2.2 1,974,054 + Pf1N1B4_1727 0.50 +0.0 1,974,055 - Pf1N1B4_1727 0.50 +0.0 1,974,204 + Pf1N1B4_1727 0.65 +0.0 1,974,204 + Pf1N1B4_1727 0.65 +0.0 1,974,204 + Pf1N1B4_1727 0.65 +0.0 1,974,204 + Pf1N1B4_1727 0.65 -2.0 1,974,204 + Pf1N1B4_1727 0.65 +0.0 1,974,205 - Pf1N1B4_1727 0.65 -1.0
Or see this region's nucleotide sequence