Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1722 and Pf1N1B4_1723 are separated by 85 nucleotides Pf1N1B4_1723 and Pf1N1B4_1724 are separated by 90 nucleotides Pf1N1B4_1724 and Pf1N1B4_1725 are separated by 3 nucleotides Pf1N1B4_1725 and Pf1N1B4_1726 are separated by 85 nucleotides
Pf1N1B4_1722 - transcriptional regulator, Crp/Fnr family, at 1,969,263 to 1,969,949
_1722
Pf1N1B4_1723 - FIG028593: membrane protein, at 1,970,035 to 1,970,553
_1723
Pf1N1B4_1724 - Sulfate and thiosulfate import ATP-binding protein CysA (EC 3.6.3.25), at 1,970,644 to 1,971,633
_1724
Pf1N1B4_1725 - Sulfate transport system permease protein CysW, at 1,971,637 to 1,972,434
_1725
Pf1N1B4_1726 - Sulfate transport system permease protein CysT, at 1,972,520 to 1,973,338
_1726
Position (kb)
1970
1971
1972 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1969.654 kb on + strand, within Pf1N1B4_1722 at 1969.654 kb on + strand, within Pf1N1B4_1722 at 1969.654 kb on + strand, within Pf1N1B4_1722 at 1969.654 kb on + strand, within Pf1N1B4_1722 at 1969.654 kb on + strand, within Pf1N1B4_1722 at 1969.655 kb on - strand, within Pf1N1B4_1722 at 1969.767 kb on - strand, within Pf1N1B4_1722 at 1969.767 kb on - strand, within Pf1N1B4_1722 at 1969.792 kb on + strand, within Pf1N1B4_1722 at 1969.793 kb on - strand, within Pf1N1B4_1722 at 1969.793 kb on - strand, within Pf1N1B4_1722 at 1969.793 kb on - strand, within Pf1N1B4_1722 at 1969.793 kb on - strand, within Pf1N1B4_1722 at 1969.824 kb on + strand, within Pf1N1B4_1722 at 1969.825 kb on - strand, within Pf1N1B4_1722 at 1969.874 kb on + strand, within Pf1N1B4_1722 at 1969.874 kb on + strand, within Pf1N1B4_1722 at 1969.874 kb on + strand, within Pf1N1B4_1722 at 1969.875 kb on - strand, within Pf1N1B4_1722 at 1969.882 kb on - strand at 1969.893 kb on + strand at 1969.893 kb on + strand at 1969.894 kb on - strand at 1969.894 kb on - strand at 1970.019 kb on + strand at 1970.020 kb on - strand at 1970.020 kb on - strand at 1970.020 kb on - strand at 1970.030 kb on + strand at 1970.030 kb on + strand at 1970.031 kb on - strand at 1970.031 kb on - strand at 1970.094 kb on + strand, within Pf1N1B4_1723 at 1970.095 kb on - strand, within Pf1N1B4_1723 at 1970.129 kb on + strand, within Pf1N1B4_1723 at 1970.129 kb on + strand, within Pf1N1B4_1723 at 1970.130 kb on - strand, within Pf1N1B4_1723 at 1970.221 kb on + strand, within Pf1N1B4_1723 at 1970.222 kb on - strand, within Pf1N1B4_1723 at 1970.222 kb on - strand, within Pf1N1B4_1723 at 1970.294 kb on + strand, within Pf1N1B4_1723 at 1970.294 kb on + strand, within Pf1N1B4_1723 at 1970.310 kb on + strand, within Pf1N1B4_1723 at 1970.310 kb on + strand, within Pf1N1B4_1723 at 1970.310 kb on + strand, within Pf1N1B4_1723 at 1970.311 kb on - strand, within Pf1N1B4_1723 at 1970.311 kb on - strand, within Pf1N1B4_1723 at 1970.311 kb on - strand, within Pf1N1B4_1723 at 1970.380 kb on + strand, within Pf1N1B4_1723 at 1970.380 kb on + strand, within Pf1N1B4_1723 at 1970.381 kb on - strand, within Pf1N1B4_1723 at 1970.381 kb on - strand, within Pf1N1B4_1723 at 1970.381 kb on - strand, within Pf1N1B4_1723 at 1970.381 kb on - strand, within Pf1N1B4_1723 at 1970.381 kb on - strand, within Pf1N1B4_1723 at 1970.383 kb on + strand, within Pf1N1B4_1723 at 1970.383 kb on + strand, within Pf1N1B4_1723 at 1970.551 kb on + strand at 1970.645 kb on + strand at 1970.645 kb on + strand at 1970.645 kb on + strand at 1970.646 kb on - strand at 1970.686 kb on + strand at 1970.687 kb on - strand at 1970.809 kb on - strand, within Pf1N1B4_1724 at 1970.809 kb on - strand, within Pf1N1B4_1724 at 1970.933 kb on + strand, within Pf1N1B4_1724 at 1971.015 kb on + strand, within Pf1N1B4_1724 at 1971.016 kb on - strand, within Pf1N1B4_1724 at 1971.386 kb on + strand, within Pf1N1B4_1724 at 1971.386 kb on + strand, within Pf1N1B4_1724 at 1971.387 kb on - strand, within Pf1N1B4_1724 at 1971.387 kb on - strand, within Pf1N1B4_1724 at 1971.387 kb on - strand, within Pf1N1B4_1724 at 1971.387 kb on - strand, within Pf1N1B4_1724 at 1971.638 kb on + strand at 1971.638 kb on + strand at 1971.638 kb on + strand at 1971.638 kb on + strand at 1971.638 kb on + strand at 1971.638 kb on + strand at 1971.639 kb on - strand at 1971.639 kb on - strand at 1971.639 kb on - strand at 1971.639 kb on - strand at 1971.639 kb on - strand at 1971.673 kb on - strand at 1971.761 kb on + strand, within Pf1N1B4_1725 at 1971.761 kb on + strand, within Pf1N1B4_1725 at 1971.761 kb on + strand, within Pf1N1B4_1725 at 1971.762 kb on - strand, within Pf1N1B4_1725 at 1971.762 kb on - strand, within Pf1N1B4_1725 at 1971.762 kb on - strand, within Pf1N1B4_1725 at 1971.770 kb on + strand, within Pf1N1B4_1725 at 1971.770 kb on + strand, within Pf1N1B4_1725 at 1971.770 kb on + strand, within Pf1N1B4_1725 at 1971.770 kb on + strand, within Pf1N1B4_1725 at 1971.771 kb on - strand, within Pf1N1B4_1725 at 1971.771 kb on - strand, within Pf1N1B4_1725 at 1971.771 kb on - strand, within Pf1N1B4_1725 at 1971.771 kb on - strand, within Pf1N1B4_1725 at 1971.771 kb on - strand, within Pf1N1B4_1725 at 1971.884 kb on + strand, within Pf1N1B4_1725 at 1971.884 kb on + strand, within Pf1N1B4_1725 at 1971.884 kb on + strand, within Pf1N1B4_1725 at 1971.884 kb on + strand, within Pf1N1B4_1725 at 1971.884 kb on + strand, within Pf1N1B4_1725 at 1971.885 kb on - strand, within Pf1N1B4_1725 at 1971.885 kb on - strand, within Pf1N1B4_1725 at 1971.885 kb on - strand, within Pf1N1B4_1725 at 1972.132 kb on + strand, within Pf1N1B4_1725 at 1972.132 kb on + strand, within Pf1N1B4_1725 at 1972.132 kb on + strand, within Pf1N1B4_1725 at 1972.132 kb on + strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.133 kb on - strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.136 kb on + strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.137 kb on - strand, within Pf1N1B4_1725 at 1972.217 kb on + strand, within Pf1N1B4_1725 at 1972.218 kb on - strand, within Pf1N1B4_1725 at 1972.218 kb on - strand, within Pf1N1B4_1725 at 1972.491 kb on + strand at 1972.491 kb on + strand at 1972.492 kb on - strand at 1972.592 kb on + strand at 1972.592 kb on + strand at 1972.592 kb on + strand at 1972.593 kb on - strand at 1972.593 kb on - strand at 1972.617 kb on + strand, within Pf1N1B4_1726 at 1972.618 kb on - strand, within Pf1N1B4_1726 at 1972.623 kb on + strand, within Pf1N1B4_1726 at 1972.624 kb on - strand, within Pf1N1B4_1726
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 1,969,654 + Pf1N1B4_1722 0.57 +0.0 1,969,654 + Pf1N1B4_1722 0.57 -1.7 1,969,654 + Pf1N1B4_1722 0.57 +0.0 1,969,654 + Pf1N1B4_1722 0.57 +0.0 1,969,654 + Pf1N1B4_1722 0.57 +0.0 1,969,655 - Pf1N1B4_1722 0.57 +0.0 1,969,767 - Pf1N1B4_1722 0.73 +0.0 1,969,767 - Pf1N1B4_1722 0.73 +0.0 1,969,792 + Pf1N1B4_1722 0.77 +0.0 1,969,793 - Pf1N1B4_1722 0.77 +0.0 1,969,793 - Pf1N1B4_1722 0.77 +0.0 1,969,793 - Pf1N1B4_1722 0.77 -2.2 1,969,793 - Pf1N1B4_1722 0.77 +0.0 1,969,824 + Pf1N1B4_1722 0.82 -1.4 1,969,825 - Pf1N1B4_1722 0.82 -2.8 1,969,874 + Pf1N1B4_1722 0.89 +0.0 1,969,874 + Pf1N1B4_1722 0.89 +0.0 1,969,874 + Pf1N1B4_1722 0.89 -1.5 1,969,875 - Pf1N1B4_1722 0.89 +0.0 1,969,882 - +0.0 1,969,893 + +0.0 1,969,893 + -1.0 1,969,894 - -2.6 1,969,894 - +0.0 1,970,019 + +0.0 1,970,020 - -2.8 1,970,020 - +0.0 1,970,020 - +0.0 1,970,030 + +0.0 1,970,030 + +0.0 1,970,031 - +0.0 1,970,031 - -3.4 1,970,094 + Pf1N1B4_1723 0.11 +0.0 1,970,095 - Pf1N1B4_1723 0.12 -1.3 1,970,129 + Pf1N1B4_1723 0.18 +0.0 1,970,129 + Pf1N1B4_1723 0.18 +0.0 1,970,130 - Pf1N1B4_1723 0.18 +0.0 1,970,221 + Pf1N1B4_1723 0.36 +0.0 1,970,222 - Pf1N1B4_1723 0.36 -1.9 1,970,222 - Pf1N1B4_1723 0.36 +0.0 1,970,294 + Pf1N1B4_1723 0.50 +0.0 1,970,294 + Pf1N1B4_1723 0.50 -4.4 1,970,310 + Pf1N1B4_1723 0.53 +0.0 1,970,310 + Pf1N1B4_1723 0.53 -1.6 1,970,310 + Pf1N1B4_1723 0.53 -2.5 1,970,311 - Pf1N1B4_1723 0.53 -1.8 1,970,311 - Pf1N1B4_1723 0.53 +0.0 1,970,311 - Pf1N1B4_1723 0.53 -2.7 1,970,380 + Pf1N1B4_1723 0.66 +0.0 1,970,380 + Pf1N1B4_1723 0.66 +0.0 1,970,381 - Pf1N1B4_1723 0.67 +0.0 1,970,381 - Pf1N1B4_1723 0.67 +0.0 1,970,381 - Pf1N1B4_1723 0.67 +0.0 1,970,381 - Pf1N1B4_1723 0.67 -1.5 1,970,381 - Pf1N1B4_1723 0.67 +0.0 1,970,383 + Pf1N1B4_1723 0.67 +0.0 1,970,383 + Pf1N1B4_1723 0.67 +0.0 1,970,551 + -2.7 1,970,645 + -0.8 1,970,645 + -3.5 1,970,645 + +0.0 1,970,646 - +0.0 1,970,686 + +0.0 1,970,687 - +0.0 1,970,809 - Pf1N1B4_1724 0.17 +0.0 1,970,809 - Pf1N1B4_1724 0.17 -4.7 1,970,933 + Pf1N1B4_1724 0.29 +0.0 1,971,015 + Pf1N1B4_1724 0.37 +0.0 1,971,016 - Pf1N1B4_1724 0.38 -6.0 1,971,386 + Pf1N1B4_1724 0.75 +0.0 1,971,386 + Pf1N1B4_1724 0.75 +0.0 1,971,387 - Pf1N1B4_1724 0.75 -3.5 1,971,387 - Pf1N1B4_1724 0.75 +0.0 1,971,387 - Pf1N1B4_1724 0.75 +0.0 1,971,387 - Pf1N1B4_1724 0.75 +0.0 1,971,638 + +0.0 1,971,638 + +0.0 1,971,638 + +0.0 1,971,638 + +0.0 1,971,638 + +0.0 1,971,638 + +0.0 1,971,639 - +0.0 1,971,639 - +0.0 1,971,639 - +0.0 1,971,639 - +0.0 1,971,639 - +0.0 1,971,673 - +0.0 1,971,761 + Pf1N1B4_1725 0.16 -4.8 1,971,761 + Pf1N1B4_1725 0.16 +0.0 1,971,761 + Pf1N1B4_1725 0.16 -1.1 1,971,762 - Pf1N1B4_1725 0.16 +0.0 1,971,762 - Pf1N1B4_1725 0.16 +0.0 1,971,762 - Pf1N1B4_1725 0.16 +0.0 1,971,770 + Pf1N1B4_1725 0.17 -3.9 1,971,770 + Pf1N1B4_1725 0.17 +0.0 1,971,770 + Pf1N1B4_1725 0.17 -3.1 1,971,770 + Pf1N1B4_1725 0.17 -3.9 1,971,771 - Pf1N1B4_1725 0.17 +0.0 1,971,771 - Pf1N1B4_1725 0.17 -3.0 1,971,771 - Pf1N1B4_1725 0.17 +0.0 1,971,771 - Pf1N1B4_1725 0.17 +0.0 1,971,771 - Pf1N1B4_1725 0.17 +0.0 1,971,884 + Pf1N1B4_1725 0.31 +0.0 1,971,884 + Pf1N1B4_1725 0.31 -3.4 1,971,884 + Pf1N1B4_1725 0.31 -4.3 1,971,884 + Pf1N1B4_1725 0.31 -5.8 1,971,884 + Pf1N1B4_1725 0.31 +0.0 1,971,885 - Pf1N1B4_1725 0.31 +0.0 1,971,885 - Pf1N1B4_1725 0.31 +0.0 1,971,885 - Pf1N1B4_1725 0.31 +0.0 1,972,132 + Pf1N1B4_1725 0.62 +0.0 1,972,132 + Pf1N1B4_1725 0.62 +0.0 1,972,132 + Pf1N1B4_1725 0.62 +0.0 1,972,132 + Pf1N1B4_1725 0.62 -5.3 1,972,133 - Pf1N1B4_1725 0.62 +0.0 1,972,133 - Pf1N1B4_1725 0.62 -3.0 1,972,133 - Pf1N1B4_1725 0.62 +0.0 1,972,133 - Pf1N1B4_1725 0.62 -2.4 1,972,133 - Pf1N1B4_1725 0.62 +0.0 1,972,133 - Pf1N1B4_1725 0.62 -3.6 1,972,133 - Pf1N1B4_1725 0.62 +0.0 1,972,133 - Pf1N1B4_1725 0.62 -3.4 1,972,133 - Pf1N1B4_1725 0.62 -3.0 1,972,133 - +0.0 1,972,133 - Pf1N1B4_1725 0.62 -3.8 1,972,133 - Pf1N1B4_1725 0.62 -4.3 1,972,133 - Pf1N1B4_1725 0.62 +0.0 1,972,133 - Pf1N1B4_1725 0.62 +0.0 1,972,133 - Pf1N1B4_1725 0.62 +0.0 1,972,133 - Pf1N1B4_1725 0.62 -4.5 1,972,133 - Pf1N1B4_1725 0.62 -4.0 1,972,133 - Pf1N1B4_1725 0.62 +0.0 1,972,133 - Pf1N1B4_1725 0.62 -4.3 1,972,136 + Pf1N1B4_1725 0.63 +0.0 1,972,136 + Pf1N1B4_1725 0.63 -4.1 1,972,136 + Pf1N1B4_1725 0.63 +0.0 1,972,136 + Pf1N1B4_1725 0.63 +0.0 1,972,136 + Pf1N1B4_1725 0.63 +0.0 1,972,136 + Pf1N1B4_1725 0.63 +0.0 1,972,136 + Pf1N1B4_1725 0.63 +0.0 1,972,136 + Pf1N1B4_1725 0.63 -4.1 1,972,136 + Pf1N1B4_1725 0.63 +0.0 1,972,137 - Pf1N1B4_1725 0.63 +0.0 1,972,137 - Pf1N1B4_1725 0.63 +0.0 1,972,137 - Pf1N1B4_1725 0.63 +0.0 1,972,137 - Pf1N1B4_1725 0.63 -3.6 1,972,137 - Pf1N1B4_1725 0.63 +0.0 1,972,137 - Pf1N1B4_1725 0.63 -4.5 1,972,137 - Pf1N1B4_1725 0.63 +0.0 1,972,137 - Pf1N1B4_1725 0.63 +0.0 1,972,137 - Pf1N1B4_1725 0.63 -3.0 1,972,137 - Pf1N1B4_1725 0.63 -3.5 1,972,217 + Pf1N1B4_1725 0.73 +0.0 1,972,218 - Pf1N1B4_1725 0.73 +0.0 1,972,218 - Pf1N1B4_1725 0.73 -3.0 1,972,491 + +0.0 1,972,491 + +0.0 1,972,492 - +0.0 1,972,592 + +0.0 1,972,592 + +0.0 1,972,592 + +0.0 1,972,593 - -2.5 1,972,593 - +0.0 1,972,617 + Pf1N1B4_1726 0.12 +0.0 1,972,618 - Pf1N1B4_1726 0.12 +0.0 1,972,623 + Pf1N1B4_1726 0.13 +0.0 1,972,624 - Pf1N1B4_1726 0.13 -3.7
Or see this region's nucleotide sequence