Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1673

Experiment: Nitrate reduction

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1672 and Pf1N1B4_1673 are separated by 91 nucleotidesPf1N1B4_1673 and Pf1N1B4_1674 overlap by 4 nucleotides Pf1N1B4_1672 - Hydrogen peroxide-inducible genes activator, at 1,915,286 to 1,916,173 _1672 Pf1N1B4_1673 - Formate dehydrogenase chain D (EC 1.2.1.2), at 1,916,265 to 1,917,104 _1673 Pf1N1B4_1674 - Putative formate dehydrogenase oxidoreductase protein, at 1,917,101 to 1,919,449 _1674 Position (kb) 1916 1917 1918Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1915.509 kb on - strand, within Pf1N1B4_1672at 1915.558 kb on + strand, within Pf1N1B4_1672at 1915.721 kb on + strand, within Pf1N1B4_1672at 1915.722 kb on - strand, within Pf1N1B4_1672at 1915.790 kb on + strand, within Pf1N1B4_1672at 1915.791 kb on - strand, within Pf1N1B4_1672at 1915.791 kb on - strand, within Pf1N1B4_1672at 1915.827 kb on + strand, within Pf1N1B4_1672at 1915.827 kb on + strand, within Pf1N1B4_1672at 1915.827 kb on + strand, within Pf1N1B4_1672at 1915.903 kb on + strand, within Pf1N1B4_1672at 1915.903 kb on + strand, within Pf1N1B4_1672at 1915.903 kb on + strand, within Pf1N1B4_1672at 1915.968 kb on + strand, within Pf1N1B4_1672at 1915.968 kb on + strand, within Pf1N1B4_1672at 1915.968 kb on + strand, within Pf1N1B4_1672at 1915.978 kb on - strand, within Pf1N1B4_1672at 1916.022 kb on + strand, within Pf1N1B4_1672at 1916.022 kb on + strand, within Pf1N1B4_1672at 1916.023 kb on - strand, within Pf1N1B4_1672at 1916.023 kb on - strand, within Pf1N1B4_1672at 1916.043 kb on + strand, within Pf1N1B4_1672at 1916.043 kb on + strand, within Pf1N1B4_1672at 1916.043 kb on + strand, within Pf1N1B4_1672at 1916.044 kb on - strand, within Pf1N1B4_1672at 1916.044 kb on - strand, within Pf1N1B4_1672at 1916.044 kb on - strand, within Pf1N1B4_1672at 1916.104 kb on + strandat 1916.147 kb on + strandat 1916.147 kb on + strandat 1916.147 kb on + strandat 1916.147 kb on + strandat 1916.148 kb on - strandat 1916.148 kb on - strandat 1916.148 kb on - strandat 1916.148 kb on - strandat 1916.148 kb on - strandat 1916.148 kb on - strandat 1916.148 kb on - strandat 1916.186 kb on - strandat 1916.200 kb on - strandat 1916.202 kb on + strandat 1916.202 kb on + strandat 1916.202 kb on + strandat 1916.202 kb on + strandat 1916.233 kb on - strandat 1916.233 kb on - strandat 1916.233 kb on - strandat 1916.239 kb on - strandat 1916.239 kb on - strandat 1916.239 kb on - strandat 1916.334 kb on + strandat 1916.334 kb on + strandat 1916.334 kb on + strandat 1916.334 kb on + strandat 1916.334 kb on + strandat 1916.334 kb on + strandat 1916.334 kb on + strandat 1916.335 kb on - strandat 1916.335 kb on - strandat 1916.340 kb on + strandat 1916.355 kb on + strand, within Pf1N1B4_1673at 1916.355 kb on + strand, within Pf1N1B4_1673at 1916.355 kb on + strand, within Pf1N1B4_1673at 1916.355 kb on + strand, within Pf1N1B4_1673at 1916.355 kb on + strand, within Pf1N1B4_1673at 1916.355 kb on + strand, within Pf1N1B4_1673at 1916.356 kb on - strand, within Pf1N1B4_1673at 1916.356 kb on - strand, within Pf1N1B4_1673at 1916.356 kb on - strand, within Pf1N1B4_1673at 1916.356 kb on - strand, within Pf1N1B4_1673at 1916.356 kb on - strand, within Pf1N1B4_1673at 1916.356 kb on - strand, within Pf1N1B4_1673at 1916.409 kb on + strand, within Pf1N1B4_1673at 1916.409 kb on + strand, within Pf1N1B4_1673at 1916.409 kb on + strand, within Pf1N1B4_1673at 1916.409 kb on + strand, within Pf1N1B4_1673at 1916.409 kb on + strand, within Pf1N1B4_1673at 1916.410 kb on - strand, within Pf1N1B4_1673at 1916.410 kb on - strand, within Pf1N1B4_1673at 1916.410 kb on - strand, within Pf1N1B4_1673at 1916.410 kb on - strand, within Pf1N1B4_1673at 1916.410 kb on - strand, within Pf1N1B4_1673at 1916.513 kb on + strand, within Pf1N1B4_1673at 1916.517 kb on + strand, within Pf1N1B4_1673at 1916.517 kb on + strand, within Pf1N1B4_1673at 1916.517 kb on + strand, within Pf1N1B4_1673at 1916.518 kb on - strand, within Pf1N1B4_1673at 1916.518 kb on - strand, within Pf1N1B4_1673at 1916.518 kb on - strand, within Pf1N1B4_1673at 1916.518 kb on - strand, within Pf1N1B4_1673at 1916.518 kb on - strand, within Pf1N1B4_1673at 1916.554 kb on - strand, within Pf1N1B4_1673at 1916.554 kb on - strand, within Pf1N1B4_1673at 1916.554 kb on - strand, within Pf1N1B4_1673at 1916.554 kb on - strand, within Pf1N1B4_1673at 1916.780 kb on - strand, within Pf1N1B4_1673at 1916.986 kb on + strand, within Pf1N1B4_1673at 1917.075 kb on + strandat 1917.076 kb on - strandat 1917.076 kb on - strandat 1917.152 kb on + strandat 1917.152 kb on + strandat 1917.152 kb on + strandat 1917.153 kb on - strandat 1917.153 kb on - strandat 1917.240 kb on + strandat 1917.241 kb on - strandat 1917.340 kb on + strand, within Pf1N1B4_1674at 1917.400 kb on + strand, within Pf1N1B4_1674at 1917.400 kb on + strand, within Pf1N1B4_1674at 1917.400 kb on + strand, within Pf1N1B4_1674at 1917.401 kb on - strand, within Pf1N1B4_1674at 1917.401 kb on - strand, within Pf1N1B4_1674at 1917.401 kb on - strand, within Pf1N1B4_1674at 1917.401 kb on - strand, within Pf1N1B4_1674at 1917.443 kb on + strand, within Pf1N1B4_1674at 1917.443 kb on + strand, within Pf1N1B4_1674at 1917.444 kb on - strand, within Pf1N1B4_1674at 1917.444 kb on - strand, within Pf1N1B4_1674at 1917.473 kb on + strand, within Pf1N1B4_1674at 1917.473 kb on + strand, within Pf1N1B4_1674at 1917.473 kb on + strand, within Pf1N1B4_1674at 1917.473 kb on + strand, within Pf1N1B4_1674at 1917.473 kb on + strand, within Pf1N1B4_1674at 1917.473 kb on + strand, within Pf1N1B4_1674at 1917.473 kb on + strand, within Pf1N1B4_1674at 1917.473 kb on + strand, within Pf1N1B4_1674at 1917.474 kb on - strand, within Pf1N1B4_1674at 1917.474 kb on - strand, within Pf1N1B4_1674at 1917.474 kb on - strand, within Pf1N1B4_1674at 1917.474 kb on - strand, within Pf1N1B4_1674at 1917.474 kb on - strand, within Pf1N1B4_1674at 1917.474 kb on - strand, within Pf1N1B4_1674at 1917.578 kb on + strand, within Pf1N1B4_1674at 1917.578 kb on + strand, within Pf1N1B4_1674at 1917.578 kb on + strand, within Pf1N1B4_1674at 1917.579 kb on - strand, within Pf1N1B4_1674at 1917.579 kb on - strand, within Pf1N1B4_1674at 1917.618 kb on - strand, within Pf1N1B4_1674at 1917.954 kb on - strand, within Pf1N1B4_1674at 1917.954 kb on - strand, within Pf1N1B4_1674at 1918.103 kb on + strand, within Pf1N1B4_1674at 1918.103 kb on + strand, within Pf1N1B4_1674at 1918.103 kb on + strand, within Pf1N1B4_1674at 1918.104 kb on - strand, within Pf1N1B4_1674

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction
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1,915,509 - Pf1N1B4_1672 0.25 -1.9
1,915,558 + Pf1N1B4_1672 0.31 +0.1
1,915,721 + Pf1N1B4_1672 0.49 +0.1
1,915,722 - Pf1N1B4_1672 0.49 +0.1
1,915,790 + Pf1N1B4_1672 0.57 -1.8
1,915,791 - Pf1N1B4_1672 0.57 +0.1
1,915,791 - Pf1N1B4_1672 0.57 -1.0
1,915,827 + Pf1N1B4_1672 0.61 +0.1
1,915,827 + Pf1N1B4_1672 0.61 +0.1
1,915,827 + Pf1N1B4_1672 0.61 +0.1
1,915,903 + Pf1N1B4_1672 0.69 -1.5
1,915,903 + Pf1N1B4_1672 0.69 +0.1
1,915,903 + Pf1N1B4_1672 0.69 -0.6
1,915,968 + Pf1N1B4_1672 0.77 +0.1
1,915,968 + Pf1N1B4_1672 0.77 -1.7
1,915,968 + Pf1N1B4_1672 0.77 +0.1
1,915,978 - Pf1N1B4_1672 0.78 +0.1
1,916,022 + Pf1N1B4_1672 0.83 +0.1
1,916,022 + Pf1N1B4_1672 0.83 +0.1
1,916,023 - Pf1N1B4_1672 0.83 +0.1
1,916,023 - Pf1N1B4_1672 0.83 +0.1
1,916,043 + Pf1N1B4_1672 0.85 +0.1
1,916,043 + Pf1N1B4_1672 0.85 +0.1
1,916,043 + Pf1N1B4_1672 0.85 -2.4
1,916,044 - Pf1N1B4_1672 0.85 +0.1
1,916,044 - Pf1N1B4_1672 0.85 +0.1
1,916,044 - Pf1N1B4_1672 0.85 -2.6
1,916,104 + -1.8
1,916,147 + +0.1
1,916,147 + +0.1
1,916,147 + +0.1
1,916,147 + +0.1
1,916,148 - -1.2
1,916,148 - +0.1
1,916,148 - +0.1
1,916,148 - -0.6
1,916,148 - +0.1
1,916,148 - +0.1
1,916,148 - +0.1
1,916,186 - -2.7
1,916,200 - +0.1
1,916,202 + +0.1
1,916,202 + +0.5
1,916,202 + +0.1
1,916,202 + +0.1
1,916,233 - +0.1
1,916,233 - +0.1
1,916,233 - +0.1
1,916,239 - +0.1
1,916,239 - +0.1
1,916,239 - +0.1
1,916,334 + +0.1
1,916,334 + -0.2
1,916,334 + -0.3
1,916,334 + +0.1
1,916,334 + +0.1
1,916,334 + -1.4
1,916,334 + +0.1
1,916,335 - +0.1
1,916,335 - -1.8
1,916,340 + +0.1
1,916,355 + Pf1N1B4_1673 0.11 -1.1
1,916,355 + Pf1N1B4_1673 0.11 -0.9
1,916,355 + Pf1N1B4_1673 0.11 +0.1
1,916,355 + Pf1N1B4_1673 0.11 +0.1
1,916,355 + Pf1N1B4_1673 0.11 +0.1
1,916,355 + Pf1N1B4_1673 0.11 +0.1
1,916,356 - Pf1N1B4_1673 0.11 +0.1
1,916,356 - Pf1N1B4_1673 0.11 +0.1
1,916,356 - Pf1N1B4_1673 0.11 +0.1
1,916,356 - Pf1N1B4_1673 0.11 +0.1
1,916,356 - Pf1N1B4_1673 0.11 +0.1
1,916,356 - Pf1N1B4_1673 0.11 +0.1
1,916,409 + Pf1N1B4_1673 0.17 +0.1
1,916,409 + Pf1N1B4_1673 0.17 +0.1
1,916,409 + Pf1N1B4_1673 0.17 +0.1
1,916,409 + Pf1N1B4_1673 0.17 +0.1
1,916,409 + Pf1N1B4_1673 0.17 +0.1
1,916,410 - Pf1N1B4_1673 0.17 +0.8
1,916,410 - Pf1N1B4_1673 0.17 +0.1
1,916,410 - Pf1N1B4_1673 0.17 -0.6
1,916,410 - Pf1N1B4_1673 0.17 +0.1
1,916,410 - Pf1N1B4_1673 0.17 +0.1
1,916,513 + Pf1N1B4_1673 0.30 +0.1
1,916,517 + Pf1N1B4_1673 0.30 +0.1
1,916,517 + Pf1N1B4_1673 0.30 +1.5
1,916,517 + Pf1N1B4_1673 0.30 +0.1
1,916,518 - Pf1N1B4_1673 0.30 +0.1
1,916,518 - Pf1N1B4_1673 0.30 +0.4
1,916,518 - Pf1N1B4_1673 0.30 +0.1
1,916,518 - Pf1N1B4_1673 0.30 +0.1
1,916,518 - Pf1N1B4_1673 0.30 -0.1
1,916,554 - Pf1N1B4_1673 0.34 -0.9
1,916,554 - Pf1N1B4_1673 0.34 +0.1
1,916,554 - Pf1N1B4_1673 0.34 -0.4
1,916,554 - Pf1N1B4_1673 0.34 +0.1
1,916,780 - Pf1N1B4_1673 0.61 +0.2
1,916,986 + Pf1N1B4_1673 0.86 -0.6
1,917,075 + +0.1
1,917,076 - +0.1
1,917,076 - +0.1
1,917,152 + +0.1
1,917,152 + -1.7
1,917,152 + -2.1
1,917,153 - +0.1
1,917,153 - +0.1
1,917,240 + +0.1
1,917,241 - +0.1
1,917,340 + Pf1N1B4_1674 0.10 +0.1
1,917,400 + Pf1N1B4_1674 0.13 +0.1
1,917,400 + Pf1N1B4_1674 0.13 +0.1
1,917,400 + Pf1N1B4_1674 0.13 +0.1
1,917,401 - Pf1N1B4_1674 0.13 -0.9
1,917,401 - Pf1N1B4_1674 0.13 +0.1
1,917,401 - Pf1N1B4_1674 0.13 +0.1
1,917,401 - Pf1N1B4_1674 0.13 +0.1
1,917,443 + Pf1N1B4_1674 0.15 +0.1
1,917,443 + Pf1N1B4_1674 0.15 -1.2
1,917,444 - Pf1N1B4_1674 0.15 +0.1
1,917,444 - Pf1N1B4_1674 0.15 -4.1
1,917,473 + Pf1N1B4_1674 0.16 +0.1
1,917,473 + Pf1N1B4_1674 0.16 +0.1
1,917,473 + Pf1N1B4_1674 0.16 -3.0
1,917,473 + Pf1N1B4_1674 0.16 +0.1
1,917,473 + Pf1N1B4_1674 0.16 +0.1
1,917,473 + Pf1N1B4_1674 0.16 +0.1
1,917,473 + Pf1N1B4_1674 0.16 +0.1
1,917,473 + Pf1N1B4_1674 0.16 -0.3
1,917,474 - Pf1N1B4_1674 0.16 -1.2
1,917,474 - Pf1N1B4_1674 0.16 -1.3
1,917,474 - Pf1N1B4_1674 0.16 +0.1
1,917,474 - Pf1N1B4_1674 0.16 +0.1
1,917,474 - Pf1N1B4_1674 0.16 +0.1
1,917,474 - Pf1N1B4_1674 0.16 -1.4
1,917,578 + Pf1N1B4_1674 0.20 +0.1
1,917,578 + Pf1N1B4_1674 0.20 +0.1
1,917,578 + Pf1N1B4_1674 0.20 +0.1
1,917,579 - Pf1N1B4_1674 0.20 +0.1
1,917,579 - Pf1N1B4_1674 0.20 +0.1
1,917,618 - Pf1N1B4_1674 0.22 +0.1
1,917,954 - Pf1N1B4_1674 0.36 -0.4
1,917,954 - Pf1N1B4_1674 0.36 +0.1
1,918,103 + Pf1N1B4_1674 0.43 +0.1
1,918,103 + Pf1N1B4_1674 0.43 -3.0
1,918,103 + Pf1N1B4_1674 0.43 -2.9
1,918,104 - Pf1N1B4_1674 0.43 +0.1

Or see this region's nucleotide sequence